##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936022_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 180201 Sequences flagged as poor quality 0 Sequence length 125 %GC 29 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.888735356629542 31.0 31.0 34.0 30.0 34.0 2 31.769629469314822 31.0 31.0 34.0 30.0 34.0 3 31.77425763453033 31.0 31.0 34.0 30.0 34.0 4 35.52466412506035 37.0 35.0 37.0 33.0 37.0 5 35.502638720095895 37.0 35.0 37.0 33.0 37.0 6 35.661383677116106 37.0 35.0 37.0 33.0 37.0 7 35.57908668653337 37.0 35.0 37.0 33.0 37.0 8 35.61878679918535 37.0 35.0 37.0 33.0 37.0 9 37.060465813175284 39.0 37.0 39.0 33.0 39.0 10-11 37.03108750783847 39.0 37.0 39.0 32.5 39.0 12-13 36.86272828674647 39.0 37.0 39.0 32.0 39.0 14-15 37.48245015288484 39.5 36.0 41.0 32.0 41.0 16-17 37.575596139866036 39.0 36.5 41.0 32.0 41.0 18-19 37.856085704296866 39.5 36.5 41.0 33.0 41.0 20-21 37.94188434026448 40.0 36.0 41.0 33.0 41.0 22-23 37.72470741005877 40.0 35.5 41.0 33.0 41.0 24-25 37.48436745634042 40.0 35.0 41.0 33.0 41.0 26-27 36.98309387850234 39.0 35.0 41.0 32.5 41.0 28-29 36.55089594397367 38.0 35.0 40.5 32.0 41.0 30-31 35.59912819573698 36.5 35.0 39.5 31.0 41.0 32-33 34.61708592072186 35.0 34.5 39.0 30.0 41.0 34-35 34.001503876227105 35.0 33.5 38.5 28.0 41.0 36-37 33.3748536356624 35.0 33.0 38.0 23.5 41.0 38-39 32.69470202718076 35.0 33.0 38.0 20.5 41.0 40-41 31.955297140415425 35.0 33.0 38.0 16.5 41.0 42-43 31.118781249826583 35.0 33.0 38.0 2.0 41.0 44-45 30.011359537405454 35.0 31.0 37.5 2.0 41.0 46-47 28.684072230453772 35.0 24.5 37.0 2.0 41.0 48-49 27.626508731916026 35.0 21.5 37.0 2.0 41.0 50-51 26.993399037741188 35.0 18.5 36.0 2.0 40.0 52-53 26.550626800073253 35.0 10.0 35.5 2.0 40.0 54-55 26.182840272806477 35.0 2.0 35.0 2.0 40.0 56-57 25.733872176070054 35.0 2.0 35.0 2.0 40.0 58-59 25.246732815023226 34.5 2.0 35.0 2.0 40.0 60-61 24.77600845722277 34.0 2.0 35.0 2.0 40.0 62-63 24.253267184976774 33.0 2.0 35.0 2.0 39.5 64-65 23.7560945832709 33.0 2.0 35.0 2.0 39.0 66-67 23.19759879245953 33.0 2.0 35.0 2.0 39.0 68-69 22.6600573803697 33.0 2.0 35.0 2.0 38.5 70-71 22.24359465263789 33.0 2.0 35.0 2.0 37.5 72-73 21.794387933474287 32.5 2.0 35.0 2.0 37.0 74-75 21.33697926204627 31.0 2.0 35.0 2.0 36.5 76-77 20.248189521700766 28.5 2.0 34.5 2.0 35.0 78-79 20.50646500296891 30.0 2.0 35.0 2.0 35.5 80-81 20.1966276546745 30.0 2.0 35.0 2.0 35.0 82-83 19.80822248489187 29.0 2.0 35.0 2.0 35.0 84-85 19.38768652782171 28.5 2.0 35.0 2.0 35.0 86-87 18.981168251008597 27.0 2.0 35.0 2.0 35.0 88-89 18.542846599075475 25.5 2.0 35.0 2.0 35.0 90-91 18.1252046326047 23.5 2.0 35.0 2.0 35.0 92-93 17.684715956071276 18.5 2.0 35.0 2.0 35.0 94-95 17.283805306296856 4.5 2.0 35.0 2.0 35.0 96-97 16.86558343183445 2.0 2.0 34.0 2.0 35.0 98-99 16.43986714835101 2.0 2.0 34.0 2.0 35.0 100-101 15.770656100687564 2.0 2.0 33.5 2.0 35.0 102-103 29.93815794584936 31.0 30.0 33.5 26.0 34.0 104-105 31.88485358016881 33.0 32.5 35.5 27.5 35.5 106-107 34.12442494769729 36.0 35.0 37.0 30.5 37.0 108-109 34.22761527405508 37.0 35.0 37.0 30.0 37.0 110-111 35.53522732948208 39.0 35.0 39.0 30.0 39.0 112-113 35.41353821565919 39.0 35.0 39.0 30.0 39.0 114-115 35.587008396179826 39.0 35.5 40.0 28.5 40.0 116-117 35.69936071386951 39.0 35.0 41.0 27.0 41.0 118-119 36.047929811710254 39.0 36.0 41.0 29.0 41.0 120-121 36.20895000582683 39.0 35.0 41.0 30.0 41.0 122-123 36.16176935755074 39.0 35.0 41.0 30.0 41.0 124-125 35.86924323394432 39.0 35.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.37764387047322145 1101 2 0.14527777475332826 1101 3 0.012465838774740945 1101 4 0.10176360668720008 1101 5 0.1472107169207888 1101 6 0.3695742057231399 1101 7 0.14477760353365454 1101 8 -0.008219894936530636 1101 9 0.11108565060546738 1101 10-11 0.3425668842018297 1101 12-13 0.025277341794712527 1101 14-15 0.2272346482788663 1101 16-17 0.24816976591601758 1101 18-19 0.27572277811106005 1101 20-21 0.3551452483481441 1101 22-23 0.5859660261560151 1101 24-25 0.3482033961465376 1101 26-27 0.7909472488598794 1101 28-29 0.5871772761948577 1101 30-31 0.40582659183336034 1101 32-33 0.21159145881188124 1101 34-35 0.6774457642114982 1101 36-37 0.868548646541953 1101 38-39 0.9709854041713797 1101 40-41 1.5784954374753468 1101 42-43 1.0161624772675744 1101 44-45 0.8063737866737846 1101 46-47 0.44189734103423106 1101 48-49 0.08105709640256364 1101 50-51 0.3797791449159327 1101 52-53 0.5118373733947976 1101 54-55 0.47746372579221585 1101 56-57 0.11318592910486913 1101 58-59 -0.15211254987030287 1101 60-61 0.06177968580892923 1101 62-63 -0.08192242664742011 1101 64-65 -0.04342351369335873 1101 66-67 0.578998352786595 1101 68-69 -0.17379218322091106 1101 70-71 -0.20460160394150506 1101 72-73 0.17568835579194442 1101 74-75 -0.0042359273632754935 1101 76-77 -0.26584038517472663 1101 78-79 -0.5920628179229688 1101 80-81 -0.6063830639464207 1101 82-83 -0.3718787319655519 1101 84-85 -0.6006611325817097 1101 86-87 -0.27746003508421424 1101 88-89 -0.4564375256635671 1101 90-91 -0.24145989126836653 1101 92-93 0.34407412270082105 1101 94-95 0.391120929090345 1101 96-97 0.3209530898717823 1101 98-99 0.49743508934608016 1101 100-101 0.12175601116253354 1101 102-103 0.9621424253055153 1101 104-105 0.7703982862553325 1101 106-107 0.356255737279362 1101 108-109 0.34533460077373235 1101 110-111 0.17062227089058268 1101 112-113 -0.006919206582658433 1101 114-115 0.4818267159830043 1101 116-117 0.7545753612534796 1101 118-119 0.4163066514464262 1101 120-121 0.63463165096816 1101 122-123 0.5827978273032457 1101 124-125 0.6653640547105155 1102 1 0.14894858469066463 1102 2 0.3088522954707038 1102 3 0.3974380094612009 1102 4 0.16979081757155257 1102 5 0.10453910282060264 1102 6 0.15961749576642603 1102 7 0.2607330766319791 1102 8 -0.1408729561610187 1102 9 0.17385621337603396 1102 10-11 -0.06281344974993885 1102 12-13 0.019093049896135028 1102 14-15 -0.08058848097283544 1102 16-17 0.20565275911329195 1102 18-19 0.017219376750517768 1102 20-21 0.1903338692510559 1102 22-23 0.42656590247017334 1102 24-25 0.561716073944929 1102 26-27 0.5308977745246892 1102 28-29 0.4433924895529273 1102 30-31 0.25121929436891577 1102 32-33 0.7202494674698912 1102 34-35 0.7952565248794059 1102 36-37 0.8824633033137488 1102 38-39 1.711554359026053 1102 40-41 1.2476358209014435 1102 42-43 1.7536392861729517 1102 44-45 2.4577343308914728 1102 46-47 1.9567396415908185 1102 48-49 1.765348994980176 1102 50-51 0.9700698066351663 1102 52-53 1.0551274166848437 1102 54-55 0.6110444308014955 1102 56-57 0.4120418351036328 1102 58-59 0.5825813276807175 1102 60-61 0.639083334541148 1102 62-63 0.6677683587576482 1102 64-65 0.32052206453793275 1102 66-67 -0.47001216050963635 1102 68-69 -0.5688138282425577 1102 70-71 -0.43929548149252184 1102 72-73 -0.514169405494389 1102 74-75 -0.4372909675611716 1102 76-77 0.06038101247524352 1102 78-79 -0.6047406163150519 1102 80-81 -0.12617279802186587 1102 82-83 -0.6778465736476775 1102 84-85 -0.46135995756624837 1102 86-87 -0.5320982540081474 1102 88-89 -0.1543348664180506 1102 90-91 -0.5905631689430741 1102 92-93 -0.47024251304438636 1102 94-95 -0.4609198872361837 1102 96-97 -0.11859545681962302 1102 98-99 0.008721422060986583 1102 100-101 0.004409072387021595 1102 102-103 0.8182030313661208 1102 104-105 0.3277575936146384 1102 106-107 0.23349754309259652 1102 108-109 0.4028485154304988 1102 110-111 0.3111603042857638 1102 112-113 0.46262315581684277 1102 114-115 0.5466071736206075 1102 116-117 0.482311910418602 1102 118-119 0.4257376965917601 1102 120-121 0.9222012242520137 1102 122-123 0.6248510122135684 1102 124-125 0.5577573756752656 1103 1 0.3145330002750768 1103 2 0.36985708015970076 1103 3 0.4461393081624969 1103 4 0.27787352433846735 1103 5 0.3954652819046771 1103 6 0.5653762107356712 1103 7 0.021925831245788174 1103 8 0.14860072804950875 1103 9 0.10481998644506518 1103 10-11 0.22025216884199494 1103 12-13 0.22850863431171575 1103 14-15 0.2873626468467094 1103 16-17 0.39080313505314734 1103 18-19 0.381411106073827 1103 20-21 0.03803550971585423 1103 22-23 0.23188886897735017 1103 24-25 0.1319484731247016 1103 26-27 0.5148776104782087 1103 28-29 0.3054909174408351 1103 30-31 0.23989837844273154 1103 32-33 0.5444548673331866 1103 34-35 0.3660870101835769 1103 36-37 0.5449718474012357 1103 38-39 0.8852129372898929 1103 40-41 0.7911252262329569 1103 42-43 1.148547010028043 1103 44-45 1.0587681654779395 1103 46-47 1.3064918630535658 1103 48-49 1.0588811207491453 1103 50-51 1.3694631764232739 1103 52-53 1.3223010325426685 1103 54-55 1.2917091608083275 1103 56-57 1.0672451843859285 1103 58-59 1.4115286961017723 1103 60-61 1.2422531910004757 1103 62-63 1.0442635740686548 1103 64-65 0.8604656735604905 1103 66-67 0.6534926241793606 1103 68-69 0.45716019773146854 1103 70-71 0.2209488794097325 1103 72-73 0.050509336815931505 1103 74-75 0.22419228602734265 1103 76-77 0.42136870898925594 1103 78-79 0.9781797527895257 1103 80-81 1.3567390269952178 1103 82-83 1.5692911570426809 1103 84-85 1.447517349337371 1103 86-87 1.3775480207697086 1103 88-89 1.5474337596926802 1103 90-91 1.1619146164294527 1103 92-93 0.6095592641258101 1103 94-95 0.8840049247938886 1103 96-97 0.6100016040142826 1103 98-99 1.0718622286228445 1103 100-101 1.1247098242667217 1103 102-103 0.5722614729245628 1103 104-105 0.40072410079166687 1103 106-107 0.1504062922381877 1103 108-109 0.19522718938812034 1103 110-111 0.6595454717498797 1103 112-113 0.47902780380044163 1103 114-115 0.5601922727320172 1103 116-117 0.3150613977733556 1103 118-119 0.0749174641925805 1103 120-121 0.2840006090777152 1103 122-123 -0.14945691669962002 1103 124-125 -0.07322348556875369 1104 1 0.027947634421419565 1104 2 0.05053743959487633 1104 3 -0.041665569886284004 1104 4 -0.26400709183611326 1104 5 -0.237557824642181 1104 6 -0.02560581750566371 1104 7 -0.035038225236881715 1104 8 0.023761190180444203 1104 9 -0.06719541791949979 1104 10-11 -0.20384924322475229 1104 12-13 -0.006247463249259511 1104 14-15 0.15024775596096163 1104 16-17 0.4055656510993586 1104 18-19 0.4026723384229882 1104 20-21 0.03635065734101062 1104 22-23 0.07567898451009114 1104 24-25 0.4130781265264929 1104 26-27 -0.06254536769894514 1104 28-29 0.18000953104802164 1104 30-31 0.5384381730512047 1104 32-33 0.525761029079014 1104 34-35 0.8542128124942252 1104 36-37 1.0257163916887961 1104 38-39 0.4616731426814198 1104 40-41 0.1771971132676171 1104 42-43 -0.15568919022869565 1104 44-45 -0.3031541708506893 1104 46-47 -0.4689413846999635 1104 48-49 -0.11016573419308884 1104 50-51 -0.3289482484804509 1104 52-53 -0.8567747055831347 1104 54-55 -1.1225238302057896 1104 56-57 -0.42914441766798817 1104 58-59 -0.2192479406119645 1104 60-61 -0.30853307344111514 1104 62-63 -0.6074533707323759 1104 64-65 -0.5932410016641576 1104 66-67 -0.25881482126351685 1104 68-69 0.44496507578024946 1104 70-71 0.01323065091165887 1104 72-73 0.38122178867729417 1104 74-75 0.17107932068716636 1104 76-77 0.30214534570298923 1104 78-79 0.2427015756393338 1104 80-81 0.42862606165504147 1104 82-83 0.3476127231530768 1104 84-85 -0.21896211150986744 1104 86-87 0.36832144824082036 1104 88-89 0.6392983296541708 1104 90-91 0.09917392323304242 1104 92-93 0.23616388543518596 1104 94-95 0.23028220335615046 1104 96-97 0.6360767002915608 1104 98-99 0.938678659945051 1104 100-101 0.7121616856274411 1104 102-103 0.6682980582904143 1104 104-105 0.8585225603552153 1104 106-107 0.49660333973498183 1104 108-109 0.7095083190415252 1104 110-111 0.3857168453057156 1104 112-113 0.5268455188196981 1104 114-115 0.5202372021286763 1104 116-117 1.0622533104819567 1104 118-119 0.6795387735635714 1104 120-121 0.8492284653036464 1104 122-123 0.8259121462015315 1104 124-125 1.054985757049991 1105 1 0.4303866588116634 1105 2 0.3066350005704841 1105 3 0.4705295520649351 1105 4 0.19940754231022595 1105 5 0.17097876072367768 1105 6 0.09634540200652708 1105 7 -0.08381871304175803 1105 8 -0.037214419575654745 1105 9 0.2681704357390302 1105 10-11 0.31139465921427245 1105 12-13 0.2498500977263447 1105 14-15 0.4429306827902266 1105 16-17 0.4878827242700936 1105 18-19 0.32645282622786453 1105 20-21 0.4753750475849188 1105 22-23 0.41104483816862114 1105 24-25 0.7423464192094187 1105 26-27 0.7148936566913022 1105 28-29 0.8263509944626577 1105 30-31 0.9012430510999891 1105 32-33 0.42515127298145217 1105 34-35 0.16213964176252205 1105 36-37 0.6354724892497714 1105 38-39 0.19033167926678374 1105 40-41 -0.47829069161043236 1105 42-43 -0.6252013853506462 1105 44-45 -1.409861487923859 1105 46-47 -0.8561365066511861 1105 48-49 -0.6254096366321136 1105 50-51 -0.9478506875048396 1105 52-53 -0.7012869007050853 1105 54-55 -0.5950848058155493 1105 56-57 -1.0236566127899387 1105 58-59 -0.990589404026597 1105 60-61 -0.8725574636850162 1105 62-63 -0.9245265414640791 1105 64-65 -0.9682410016641576 1105 66-67 -0.6795465285805875 1105 68-69 -0.6800349242197505 1105 70-71 -0.483720568600539 1105 72-73 -0.6248757722983171 1105 74-75 -1.1947743378494202 1105 76-77 -1.106391239662866 1105 78-79 -1.6414447658240796 1105 80-81 -1.748203206637644 1105 82-83 -1.847509228066432 1105 84-85 -1.597010891997673 1105 86-87 -1.3847273322469817 1105 88-89 -1.5375309386385112 1105 90-91 -1.8825333938401236 1105 92-93 -1.6296897731014006 1105 94-95 -1.4861812112779962 1105 96-97 -1.5742891533669763 1105 98-99 -2.064370120542751 1105 100-101 -1.986009046079877 1105 102-103 1.1774443997538313 1105 104-105 0.6969371945015546 1105 106-107 0.7862374860764376 1105 108-109 0.8070692946512779 1105 110-111 0.9101070892081609 1105 112-113 1.0634308846733518 1105 114-115 1.0964567143238 1105 116-117 1.0195703836526846 1105 118-119 0.6277095052708859 1105 120-121 0.30654553847437427 1105 122-123 0.5362779998600686 1105 124-125 0.8568150253426694 1106 1 0.1669426388292905 1106 2 0.2748982976612844 1106 3 -0.03277635448810656 1106 4 0.1783966695508994 1106 5 0.21704105869605428 1106 6 0.0990635918390268 1106 7 -0.240518683655921 1106 8 -0.008342835679087557 1106 9 -0.3324025880634949 1106 10-11 -0.12700528201405348 1106 12-13 0.17278574274545377 1106 14-15 0.1509089372715735 1106 16-17 -0.04951075207431188 1106 18-19 0.2784069843236665 1106 20-21 0.05717786862517471 1106 22-23 0.05936984551507862 1106 24-25 -0.36630770950053915 1106 26-27 -0.194340842280198 1106 28-29 -0.4797024877589138 1106 30-31 -0.40646338439809426 1106 32-33 0.029434258582391237 1106 34-35 0.6410553044131291 1106 36-37 0.6673561213390968 1106 38-39 0.5496469892873534 1106 40-41 0.484609690405442 1106 42-43 1.3581221527040093 1106 44-45 0.7098857938863077 1106 46-47 1.0593057501810215 1106 48-49 0.5924496052133144 1106 50-51 0.5197881605703856 1106 52-53 1.108366346429797 1106 54-55 1.3955851912458677 1106 56-57 1.6346595788057137 1106 58-59 1.7482439782831278 1106 60-61 1.436728460499527 1106 62-63 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118-119 -0.24948224475721048 2314 120-121 -0.4005888888606748 2314 122-123 -0.37221643646677904 2314 124-125 -0.7987570776133879 2315 1 -0.2363519554771365 2315 2 -0.7036175355831951 2315 3 -0.3790819307755555 2315 4 -0.22148604715663822 2315 5 -0.17399210048936453 2315 6 -0.3683103286215754 2315 7 -0.8908012297695791 2315 8 -0.5037048145680956 2315 9 -0.1369126637645266 2315 10-11 -0.5780128521174817 2315 12-13 -0.23941171967058494 2315 14-15 -0.49620162888687247 2315 16-17 -0.471970502580767 2315 18-19 -0.8383432022633883 2315 20-21 -1.0181766467815834 2315 22-23 -1.1009884016328044 2315 24-25 -0.963999361062541 2315 26-27 -1.727233474757 2315 28-29 -0.978742897184226 2315 30-31 -0.34193091825032695 2315 32-33 -0.23794499293695992 2315 34-35 0.18868831432889976 2315 36-37 -0.4195134809639214 2315 38-39 -0.7718958191143983 2315 40-41 -1.3141961524349526 2315 42-43 -1.55580790380521 2315 44-45 -1.2097751507773005 2315 46-47 -1.1974380391355375 2315 48-49 -1.0298182271781968 2315 50-51 -0.8550652353140364 2315 52-53 -0.8186514593064231 2315 54-55 -0.9070856691870155 2315 56-57 -0.7661560731827741 2315 58-59 -0.9284806192219364 2315 60-61 -0.6841266413236937 2315 62-63 -0.10516004027694592 2315 64-65 0.09232990271745223 2315 66-67 0.1745976544541641 2315 68-69 0.7735318791473986 2315 70-71 0.7914251253342783 2315 72-73 0.6122815771512826 2315 74-75 0.7988313172336774 2315 76-77 0.34655501546340517 2315 78-79 0.1641077919138887 2315 80-81 0.2322630139537658 2315 82-83 0.09278323901752827 2315 84-85 -0.4121414769318399 2315 86-87 -0.2979584999568914 2315 88-89 -0.1812283269398307 2315 90-91 -0.5156005209715708 2315 92-93 -0.4400448346166179 2315 94-95 -0.2415772829378291 2315 96-97 -0.30613137223164344 2315 98-99 -0.4382099651358864 2315 100-101 -0.7357932032134844 2315 102-103 0.33719510124314667 2315 104-105 0.1207986233813294 2315 106-107 0.01524676731969521 2315 108-109 -0.13208999738412075 2315 110-111 -0.15505047608430544 2315 112-113 -0.02085575465321199 2315 114-115 -0.556656819023921 2315 116-117 -0.6539942612858027 2315 118-119 -0.6862609242564162 2315 120-121 -0.38871131453662855 2315 122-123 -0.06314462047232183 2315 124-125 -0.1934493821686658 2316 1 -0.7254213376244678 2316 2 -1.1151825737696406 2316 3 -1.0641346644402425 2316 4 -0.6769149880383623 2316 5 -0.7843893203543928 2316 6 -0.5182274902977397 2316 7 -0.47576102287581534 2316 8 -0.37603277351261966 2316 9 -0.8967421380442389 2316 10-11 -1.1260287732392342 2316 12-13 -0.8028840597522091 2316 14-15 -1.2572029623818395 2316 16-17 -1.4445429356029464 2316 18-19 -0.941032417117718 2316 20-21 -0.8594200298179047 2316 22-23 -0.814509232440578 2316 24-25 -0.6264028847219762 2316 26-27 -0.3645834565729871 2316 28-29 -0.3043985322106977 2316 30-31 -1.5059710039172742 2316 32-33 -1.1602285020491792 2316 34-35 -0.745394604812823 2316 36-37 -1.8289166409429072 2316 38-39 -1.2547624292085473 2316 40-41 -1.08495624456733 2316 42-43 -1.2319504665400878 2316 44-45 -1.156910157040688 2316 46-47 -1.2945205137789237 2316 48-49 -2.0396679059271356 2316 50-51 -2.4625934205486075 2316 52-53 -1.9441313154506012 2316 54-55 -1.4836303008595415 2316 56-57 -1.5980245824970325 2316 58-59 -1.9174186723192825 2316 60-61 -2.246346304313146 2316 62-63 -1.8134479135545156 2316 64-65 -1.7848269243726982 2316 66-67 -2.308430591393819 2316 68-69 -2.4983192543233272 2316 70-71 -1.8769993301552788 2316 72-73 -1.5964204877454726 2316 74-75 -1.2910684685989473 2316 76-77 -1.733131411174167 2316 78-79 -1.3621681203179001 2316 80-81 -1.1265582573114372 2316 82-83 -0.8415926449279993 2316 84-85 -0.7552133644333558 2316 86-87 -0.5396443608693247 2316 88-89 -1.4008509074546396 2316 90-91 -1.02088398967485 2316 92-93 -1.3217072583447482 2316 94-95 -1.4731508070013497 2316 96-97 -1.5193693404702167 2316 98-99 -1.6221716574622302 2316 100-101 -1.4729229563148696 2316 102-103 -1.0541654677147072 2316 104-105 -1.0384732075476713 2316 106-107 -1.4138961590956285 2316 108-109 -1.80194897018891 2316 110-111 -1.6729617380554558 2316 112-113 -2.2989702735848 2316 114-115 -2.514399171632313 2316 116-117 -1.9961468454374156 2316 118-119 -2.132244275773779 2316 120-121 -1.6282049905377605 2316 122-123 -1.9147326917536915 2316 124-125 -1.7753684026559924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 3.0 4 5.0 5 20.0 6 48.0 7 84.0 8 168.0 9 249.0 10 365.0 11 492.0 12 705.0 13 1013.0 14 1439.0 15 2068.0 16 2917.0 17 4304.0 18 5510.0 19 6911.0 20 8914.0 21 10217.0 22 7226.0 23 4780.0 24 4466.0 25 4427.0 26 4392.0 27 4438.0 28 4868.0 29 4995.0 30 5522.0 31 5774.0 32 6272.0 33 7026.0 34 8533.0 35 16436.0 36 20612.0 37 10734.0 38 12465.0 39 1803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 52.388485534758765 20.934079353449235 16.43964119984902 10.237793911942983 2 15.871430315541794 27.94703603813583 39.69244941675269 16.489084229569695 3 19.699113767404178 39.738403227507064 30.372750428687965 10.189732576400797 4 10.691809938177741 24.259964260741203 33.42397638101158 31.62424942006948 5 8.164216624769008 46.679541179016766 27.87775872497933 17.2784834712349 6 19.697448959772696 41.157929201280794 18.736300020532628 20.40832181841388 7 18.936631872187167 46.722826177435195 18.858940849384854 15.481601100992782 8 28.16743525285653 32.01758036858841 19.26237923207973 20.552605146475326 9 31.524797309670866 17.78347511944995 19.393344099089347 31.298383471789837 10-11 23.588381862475792 34.88965100082685 20.9233023124178 20.598664824279556 12-13 29.272312584280886 35.651855428105286 25.004578220986566 10.071253766627267 14-15 23.793985604963346 33.11690834124117 32.33528115826216 10.75382489553332 16-17 12.794359616094294 37.55594462834454 32.11700300498611 17.532692750575055 18-19 12.680007325153579 29.174366401962253 45.920111431124134 12.225514841760035 20-21 15.5338512763596 27.68507214206437 47.340732519422865 9.440344062153164 22-23 13.574286491195942 30.439065266008512 47.38042519186908 8.606223050926465 24-25 12.297933973729336 32.02534947086864 47.23003756915888 8.446678986243139 26-27 11.797104344592983 32.88050565757127 46.94951748325481 8.372872514580939 28-29 10.3700867364776 33.74620562593992 47.45617393910134 8.42753369848114 30-31 11.107843082848216 34.40401338535048 46.93946070134353 7.5486828304577775 32-33 9.660681633411858 35.40131909356019 46.76788781322922 8.170111459798724 34-35 9.376474048423706 35.757570712704144 46.541084677665495 8.324870561206653 36-37 9.644668642271279 35.764825358772015 45.931974827689544 8.658531171267162 38-39 9.801277462389221 35.26173550646223 45.73087829701278 9.206108734135771 40-41 9.318483249260549 35.54614014350641 45.71395275275942 9.421423854473614 42-43 9.621531631520533 35.54661487236404 45.015538290788015 9.816315205327413 44-45 9.228028701283566 36.04530496501129 43.74920227967659 10.977464054028557 46-47 9.582910194726999 35.71678292573293 42.0474914123673 12.652815467172768 48-49 9.760212207479425 35.793641544719506 40.72646655678936 13.719679691011704 50-51 9.614264071786504 36.33581389670423 39.54750528576423 14.502416745745029 52-53 9.538317253932448 36.29116617970638 39.29089703356558 14.879619532795596 54-55 9.322645268339242 35.904073784274225 38.46621272911915 16.30706821826738 56-57 9.681688780861371 35.60496334648531 37.49202279676583 17.22132507588748 58-59 9.153995560488347 35.088512763596 38.37319644839068 17.384295227524973 60-61 10.026303960577355 34.83748702837387 38.14407245242813 16.99213655862065 62-63 9.931465038845728 34.82241953385128 38.82019977802442 16.42591564927858 64-65 9.916426656899795 34.43793319681911 39.68929140237846 15.956348743902643 66-67 9.671422467133922 34.48538021431624 39.785017841188456 16.05817947736139 68-69 9.787959001337395 34.146036925433265 39.90349665096198 16.162507422267357 70-71 9.844007524930493 33.51174521784008 39.92569408604836 16.718553171181068 72-73 9.735267105252206 32.82871024220243 39.95688136270432 17.47914128984104 74-75 9.926415502688664 32.061420302884 39.928746233372735 18.0834179610546 76-77 9.523809523809524 31.822242939828303 40.82996209787959 17.823985438482584 78-79 9.515208017713553 31.83500646500297 40.55859845394865 18.091187063334832 80-81 9.422533726227934 32.162418632527014 39.721477683253696 18.693569957991354 82-83 9.5460069588959 31.854429220703544 39.1543332167968 19.445230603603754 84-85 9.75549525252357 31.44044705634264 38.716211341779456 20.087846349354333 86-87 10.002441717859503 31.42629619147507 38.33191824684658 20.239343843818848 88-89 10.290453438105226 31.188506168112273 37.87215387262002 20.64888652116248 90-91 10.374947627200005 31.207806946228743 37.37433996398416 21.042905462587093 92-93 10.539591102823655 31.019135144734506 36.89148241875333 21.54979133368851 94-95 10.497999456162841 31.148550784956797 36.41988668209388 21.933563076786477 96-97 10.657325194228635 31.298002219755826 35.696170921198664 22.34850166481687 98-99 10.762020771538054 31.480561713887738 35.34352394720267 22.413893567371538 100-101 10.638982688727278 31.584540553439084 35.33314280482019 22.44333395301345 102-103 35.92952480869949 24.120378465543705 26.905025576121922 13.045071149634884 104-105 15.760462289130544 32.28391258250207 30.67328348515379 21.2823416432136 106-107 14.520475708080655 43.81510109490571 23.02743508971566 18.636988107297984 108-109 25.001873184052126 39.294406256157224 18.59863632201005 17.105084237780595 110-111 28.27545634667362 28.05761983782974 19.714065301002883 23.95285851449376 112-113 27.164200641573522 37.270093572054925 20.889156519519155 14.676549266852405 114-115 28.411623176420637 33.64821325992302 26.62085922258699 11.319304341069353 116-117 19.008367678281505 37.4983000985249 30.72089699290898 12.772435230284612 118-119 13.266053823607779 35.24763522515321 34.35695887734257 17.129352073896438 120-121 15.259270772330758 32.08335299293087 44.123353103949775 8.534023130788595 122-123 16.412517725674547 32.29610964803956 42.52746612349557 8.76390650279032 124-125 13.653051540269564 34.64545078657672 42.76429893522848 8.937198737925236 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 6846.0 1 4274.0 2 2275.0 3 2722.5 4 2090.0 5 1808.5 6 3184.0 7 5965.0 8 7848.5 9 7212.0 10 4861.0 11 3068.0 12 2634.0 13 2408.5 14 2095.0 15 1660.0 16 1343.0 17 1217.0 18 1122.0 19 1081.0 20 1109.0 21 1150.5 22 1235.5 23 1370.5 24 1514.0 25 1721.0 26 2077.5 27 2463.0 28 2818.5 29 3271.0 30 3744.0 31 4177.0 32 4613.0 33 4998.0 34 5298.5 35 5401.0 36 5284.0 37 5082.0 38 4668.0 39 4329.5 40 4089.5 41 3803.0 42 3613.5 43 3431.0 44 3260.5 45 3066.5 46 2936.0 47 2913.0 48 3331.0 49 3325.0 50 3138.0 51 3115.0 52 2478.5 53 1843.5 54 1376.5 55 1072.5 56 874.5 57 688.0 58 545.0 59 492.5 60 517.0 61 522.0 62 376.0 63 182.0 64 118.5 65 90.5 66 65.0 67 58.0 68 45.0 69 30.5 70 25.0 71 24.5 72 17.0 73 14.0 74 12.0 75 17.0 76 16.5 77 5.5 78 13.5 79 20.0 80 12.0 81 5.0 82 1.0 83 1.5 84 1.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02497211447217274 2 0.0016648076314781826 3 0.0 4 0.0038845511401157597 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.7746793857969714E-4 18-19 0.0 20-21 5.549358771593943E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.002219743508637577 32-33 8.324038157390913E-4 34-35 0.0 36-37 0.0016648076314781826 38-39 0.0 40-41 0.0 42-43 5.549358771593943E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.324038157390913E-4 54-55 0.0 56-57 0.0 58-59 5.549358771593943E-4 60-61 0.0 62-63 5.549358771593943E-4 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.7746793857969714E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0013873396928984856 92-93 0.004994422894434547 94-95 0.0 96-97 5.549358771593943E-4 98-99 0.0013873396928984856 100-101 2.7746793857969714E-4 102-103 0.029134133550868194 104-105 0.029966537366607288 106-107 0.02691439004223062 108-109 0.014705800744723947 110-111 0.011098717543187885 112-113 0.01054378166602849 114-115 0.014705800744723947 116-117 0.02413971065643365 118-119 0.03163134499808547 120-121 0.028579197673708805 122-123 0.013595928990405159 124-125 0.011931121358926976 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 180201.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.740068035138535 #Duplication Level Percentage of deduplicated Percentage of total 1 80.73507233424566 32.08417267384754 2 10.217561302574987 8.120931626350576 3 3.6404513210076526 4.340153495263623 4 1.6701111545551026 2.654813236330542 5 0.8937049656482154 1.7757948069100618 6 0.5850974696978161 1.395108795178717 7 0.38680668044461824 1.0760206658120655 8 0.27788638775624197 0.8834579164377555 9 0.19968720326202313 0.7142024739041404 >10 1.157627213316204 9.00494447866549 >50 0.1256772607942803 3.4788930139122423 >100 0.09076691057364687 7.334032552538554 >500 0.00418924202647601 1.1431679069483522 >1k 0.009774898061777356 7.787415164177778 >5k 0.00418924202647601 12.380064483548926 >10k+ 0.0013964140088253366 5.8268267101736395 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10500 5.8268267101736395 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 8035 4.4589097729757325 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7706 4.276335869390293 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6568 3.6448188411829014 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3631 2.0149721699657603 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2612 1.4494925111403376 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2475 1.3734662959695008 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1621 0.8995510568753781 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1385 0.768586189865761 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1199 0.6653681167141137 No Hit CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT 1110 0.6159788236469276 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 778 0.4317401124300087 No Hit ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT 669 0.37125210181963475 RNA PCR Primer, Index 17 (95% over 21bp) GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 613 0.3401756926987087 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 478 0.2652593492821904 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 444 0.24639152945877102 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.23029838902114858 No Hit ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCT 410 0.22752370963535162 RNA PCR Primer, Index 17 (95% over 23bp) GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 397 0.22030954323227953 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 392 0.21753486384648255 No Hit GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 365 0.2025515951631789 No Hit TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG 356 0.19755717226874436 RNA PCR Primer, Index 17 (96% over 28bp) TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 345 0.191452877619991 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 344 0.19089794174283162 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 326 0.1809090959539625 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 305 0.16925544253361524 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 282 0.15649191735894916 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 279 0.154827109727471 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 269 0.14927775095587703 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 263 0.14594813569292067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 248 0.1376240975355298 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 237 0.13151980288677645 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 231 0.12819018762382006 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCT 224 0.12430563648370431 No Hit TATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA 222 0.12319576472938551 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 213 0.11820134183495097 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 210 0.11653653420347278 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 207 0.11487172657199461 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 201 0.11154211130903824 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 194 0.10765756016892249 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 193 0.10710262429176308 No Hit GTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 191 0.10599275253744429 No Hit GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA 190 0.1054378166602849 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 185 0.10266313727448793 No Hit CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCT 184 0.10210820139732854 RNA PCR Primer, Index 17 (96% over 26bp) >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0013873396928984856 0.0 20-21 0.0 0.0 0.0 0.002774679385796971 0.0 22-23 0.0 0.0 0.0 0.010821249604608188 0.0 24-25 0.0 0.0 0.0 0.027191857980810315 0.0 26-27 0.0 0.0 0.0 0.05910067091747549 0.0 28-29 0.0 0.0 0.0 0.10294060521306764 0.0 30-31 0.0 0.0 0.0 0.20005438371596163 0.0 32-33 0.0 0.0 0.0 0.30022030954323226 0.0 34-35 0.0 0.0 0.0 0.42813302922847263 0.0 36-37 0.0 0.0 0.0 0.6154238877697682 0.0 38-39 0.0 0.0 0.0 0.8387855783264244 0.0 40-41 0.0 0.0 0.0 1.0829573642765578 0.0 42-43 0.0 0.0 0.0 1.252212806810173 0.0 44-45 0.0 0.0 0.0 1.394276391362978 0.0 46-47 0.0 0.0 0.0 1.5593698148178978 0.0 48-49 0.0 0.0 0.0 1.735839423754585 0.0 50-51 0.0 0.0 0.0 1.9006553792709253 0.0 52-53 0.0 0.0 0.0 2.056592360752715 0.0 54-55 0.0 0.0 0.0 2.1983784773669406 0.0 56-57 0.0 0.0 0.0 2.329343344376557 0.0 58-59 0.0 0.0 0.0 2.482228178533971 0.0 60-61 0.0 0.0 0.0 2.6270664424725725 0.0 62-63 0.0 0.0 0.0 2.762748264438044 0.0 64-65 0.0 0.0 0.0 2.913968290963979 0.0 66-67 0.0 0.0 0.0 3.0729574197701455 0.0 68-69 0.0 0.0 0.0 3.2424903302423407 0.0 70-71 0.0 0.0 0.0 3.455030771194388 0.0 72-73 0.0 0.0 0.0 3.672843102979451 0.0 74-75 0.0 0.0 0.0 3.872897486695412 0.0 76-77 0.0 0.0 0.0 4.08543792764746 0.0 78-79 0.0 0.0 0.0 4.307134810572638 0.0 80-81 0.0 0.0 0.0 4.559075698803003 0.0 82-83 0.0 0.0 0.0 4.821837836637977 0.0 84-85 0.0 0.0 0.0 5.084599974472949 0.0 86-87 0.0 0.0 0.0 5.348194516123662 0.0 88-89 0.0 0.0 0.0 5.6031875516784035 0.0 90-91 0.0 0.0 0.0 5.830988729252335 0.0 92-93 0.0 0.0 0.0 5.850411484952914 0.0 94-95 0.0 0.0 0.0 5.851243888768653 0.0 96-97 0.0 0.0 0.0 5.851798824645813 0.0 98-99 0.0 0.0 0.0 5.851798824645813 0.0 100-101 0.0 0.0 0.0 5.851798824645813 0.0 102-103 0.0 0.0 0.0 5.851798824645813 0.0 104-105 0.0 0.0 0.0 5.851798824645813 0.0 106-107 0.0 0.0 0.0 5.851798824645813 0.0 108-109 0.0 0.0 0.0 5.851798824645813 0.0 110-111 0.0 0.0 0.0 5.851798824645813 0.0 112-113 0.0 0.0 0.0 5.851798824645813 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCCAT 25 0.0020544247 71.39651 5 AACGTAT 45 2.3646862E-11 59.5136 98-99 TCGCAGA 35 1.3358658E-8 59.5136 100-101 CGCGGTA 30 3.142104E-7 59.5136 98-99 TAGACGT 15 0.004230364 59.5136 96-97 TCCGACG 15 0.004230364 59.5136 96-97 TGCGAGT 15 0.004230364 59.5136 98-99 TTCGGTA 15 0.004230364 59.5136 98-99 CCGGTAC 15 0.004230364 59.5136 98-99 CCCGGTA 115 0.0 59.5136 98-99 GCGTCAC 15 0.004230364 59.5136 100-101 GCCGTAC 30 3.142104E-7 59.5136 98-99 GCGTACA 35 1.3358658E-8 59.5136 100-101 TACCGAG 15 0.004230364 59.5136 98-99 AACGTAC 20 1.7683425E-4 59.513596 98-99 ACCGGTA 50 1.8189894E-12 59.513596 98-99 TTTAACG 25 7.431574E-6 59.513596 98-99 CCGTAAT 20 1.7683425E-4 59.513596 100-101 GTAGACG 20 1.7683425E-4 59.513596 96-97 TTACGAG 20 1.7683425E-4 59.513596 98-99 >>END_MODULE