FastQCFastQC Report
Thu 26 May 2016
SRR936021_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936021_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518262
Sequences flagged as poor quality0
Sequence length77
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99530.39523290269241246No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35780.14208211854048544No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA5950.028.63588559
ATTCGCG6100.027.35198456
CGATCGT6500.027.302881
TCGCGGA6750.026.29375358
TCGCGCT6700.025.96069137
CTCGCGC6800.025.57840536
AATTCGC6750.024.71710855
ATCGTTC7500.024.1352673
GATCGTT7400.023.9817832
CGGTTAC5750.023.4558034
CGCGCTT7450.023.3462738
CAATTCG7200.023.1755154
CCTCGCG8000.023.0718535
CGCTCCG7650.022.73545825
AACTCGA8100.022.34924313
ACTCGAG8400.021.97362714
GCCTAGT7500.019.4063317
GACGCTC9400.019.2599223
GCCCTCG9700.019.02795233
GCGCCCT9200.018.90465431
Produced by FastQC (version 0.11.5)