##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936021_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2518262 Sequences flagged as poor quality 0 Sequence length 77 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.443949041044974 39.0 36.0 41.0 30.0 41.0 2 36.2936386285462 39.0 36.0 41.0 29.0 41.0 3 36.20490242873855 39.0 36.0 41.0 29.0 41.0 4 36.07436279465759 39.0 35.0 41.0 29.0 41.0 5 35.98956145150902 39.0 35.0 41.0 28.0 41.0 6 35.86808044595836 39.0 35.0 40.0 28.0 41.0 7 35.6853194782751 39.0 35.0 40.0 27.0 41.0 8 35.49176813214828 38.0 35.0 40.0 27.0 41.0 9 35.375784171781966 38.0 35.0 40.0 27.0 41.0 10-11 35.56176104789732 39.0 35.0 40.0 27.0 41.0 12-13 35.7197189966731 39.0 35.0 41.0 27.5 41.0 14-15 35.65016944225819 39.0 35.0 41.0 27.0 41.0 16-17 35.44904620726517 39.0 35.0 41.0 26.5 41.0 18-19 35.24713334037523 39.0 35.0 41.0 26.0 41.0 20-21 34.97868788076856 39.0 34.5 41.0 24.5 41.0 22-23 34.63194258579925 38.5 34.0 41.0 23.5 41.0 24-25 34.334809880782856 38.0 34.0 40.0 22.5 41.0 26-27 33.356633860972366 37.5 32.0 39.5 20.0 40.5 28-29 33.352851291883056 37.5 33.0 39.5 20.0 40.5 30-31 33.67417548293228 38.0 33.0 40.0 19.5 41.0 32-33 33.491482419224056 38.0 33.0 40.0 18.0 41.0 34-35 33.19507342762588 37.0 33.0 40.0 15.5 41.0 36-37 32.88065757256393 37.0 32.0 40.0 13.0 41.0 38-39 32.57714626198545 36.0 32.0 40.0 10.5 41.0 40-41 32.17888190347152 36.0 31.5 39.0 8.0 41.0 42-43 31.803652677918343 35.0 31.0 39.0 7.0 41.0 44-45 31.432271939933177 35.0 31.0 38.5 2.0 40.0 46-47 31.03560491322984 35.0 31.0 37.5 2.0 40.0 48-49 30.58732987274557 35.0 30.0 37.0 2.0 39.0 50-51 30.160321880725675 35.0 30.0 36.5 2.0 39.0 52-53 29.724307677279015 34.5 29.5 36.0 2.0 38.5 54-55 29.284125122802948 34.0 29.0 35.5 2.0 37.5 56-57 28.8601321069849 34.0 29.0 35.0 2.0 37.0 58-59 28.459075147859913 34.0 28.5 35.0 2.0 36.5 60-61 28.126599813681022 34.0 28.0 35.0 2.0 36.0 62-63 27.79814729364935 34.0 27.0 35.0 2.0 36.0 64-65 27.435486259968187 34.0 26.5 35.0 2.0 35.0 66-67 27.071178654167042 33.0 26.0 35.0 2.0 35.0 68-69 26.723322473991985 33.0 25.0 35.0 2.0 35.0 70-71 26.299984076319305 33.0 24.5 35.0 2.0 35.0 72-73 25.81000289088268 33.0 23.5 35.0 2.0 35.0 74-75 25.223632608521275 33.0 19.0 35.0 2.0 35.0 76-77 23.554116886964103 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.844677921445907 1101 2 1.0860158031949396 1101 3 0.9474287397752974 1101 4 1.0057552076940084 1101 5 1.137153649039739 1101 6 0.8132464208835088 1101 7 0.8987581901389916 1101 8 1.242697600084746 1101 9 0.9623503668495914 1101 10-11 0.9406176376417577 1101 12-13 0.9377994621355299 1101 14-15 0.943300977392667 1101 16-17 1.078549603634599 1101 18-19 1.025823569801851 1101 20-21 1.072550536863048 1101 22-23 1.0333556014333425 1101 24-25 0.9896040000265245 1101 26-27 0.7333634683600891 1101 28-29 0.8168646595982096 1101 30-31 0.9088284498399091 1101 32-33 0.9860727607999777 1101 34-35 0.7904536465013976 1101 36-37 0.8080896988232027 1101 38-39 0.7821049174024708 1101 40-41 0.7428832505375951 1101 42-43 0.7077194700385618 1101 44-45 0.7391562532990186 1101 46-47 0.7201606203054816 1101 48-49 0.792522334695601 1101 50-51 0.8724957990162814 1101 52-53 0.8956393694385945 1101 54-55 0.7274172965020362 1101 56-57 0.6444285592473094 1101 58-59 0.6734886092355978 1101 60-61 0.5114958427130745 1101 62-63 0.3740634971999839 1101 64-65 0.1941186816871756 1101 66-67 0.274204628257543 1101 68-69 0.4628222150116983 1101 70-71 0.31105297959642186 1101 72-73 0.22750709004018077 1101 74-75 0.29646436641078466 1101 76-77 0.4417682376707859 1102 1 0.8821153631923337 1102 2 1.0307322744041016 1102 3 0.8837879523554477 1102 4 0.8200385156359573 1102 5 0.9340378081856926 1102 6 1.0378010734957002 1102 7 0.8579501826825364 1102 8 0.9858606427085803 1102 9 1.072581659264742 1102 10-11 0.9056826196182399 1102 12-13 1.0121113681362601 1102 14-15 0.9810956141336931 1102 16-17 1.05907363311605 1102 18-19 1.0980774636671171 1102 20-21 1.2012072703209071 1102 22-23 1.148917586977099 1102 24-25 1.0597339078314718 1102 26-27 0.7556197953062807 1102 28-29 0.8129862350368171 1102 30-31 0.9619971634138835 1102 32-33 0.7915952930008743 1102 34-35 0.8043464449562023 1102 36-37 0.9021665504913656 1102 38-39 0.9376414866086478 1102 40-41 0.9651890813224355 1102 42-43 0.9321345012381457 1102 44-45 1.0237763745038855 1102 46-47 0.9116684192656201 1102 48-49 0.8893030879025865 1102 50-51 0.9646789650909007 1102 52-53 0.8769956997536603 1102 54-55 0.8484198381357935 1102 56-57 0.9813830344317545 1102 58-59 0.8993020069020901 1102 60-61 0.7941133511633502 1102 62-63 0.9312878759221306 1102 64-65 0.9583874847236586 1102 66-67 0.8773287977347337 1102 68-69 0.7567066661273607 1102 70-71 0.8362511821111056 1102 72-73 0.6965204944444316 1102 74-75 0.6988013406313556 1102 76-77 0.7943311140964759 1103 1 0.42391351718298154 1103 2 0.31282378473642325 1103 3 0.35989735626922936 1103 4 0.49396293472815955 1103 5 0.4716087386631074 1103 6 0.4752974629131117 1103 7 0.48055867253656714 1103 8 0.4640229074150639 1103 9 0.5520739228344738 1103 10-11 0.5361590115327743 1103 12-13 0.5579042707092299 1103 14-15 0.581594200648837 1103 16-17 0.5857375293399869 1103 18-19 0.6517127379875376 1103 20-21 0.8307721182478218 1103 22-23 0.7151477846786207 1103 24-25 0.7353828343470283 1103 26-27 0.6035553806492686 1103 28-29 0.6438222875659037 1103 30-31 0.7907066526771729 1103 32-33 0.8772256401572136 1103 34-35 0.8169924289634167 1103 36-37 0.7695021921128529 1103 38-39 0.7147236513555768 1103 40-41 0.6830028106675741 1103 42-43 0.8161385488177046 1103 44-45 0.8639224190757098 1103 46-47 0.6515303692224812 1103 48-49 0.694930472051805 1103 50-51 0.6790741482476044 1103 52-53 0.7443635803836095 1103 54-55 0.7612647535969153 1103 56-57 0.8643839456962148 1103 58-59 0.727296088341923 1103 60-61 0.7566329880738465 1103 62-63 0.758153498889719 1103 64-65 0.6252059977413218 1103 66-67 0.7174946888637876 1103 68-69 0.7046825036427578 1103 70-71 0.7548985600361426 1103 72-73 0.847632833412451 1103 74-75 0.7216832098229737 1103 76-77 0.576603106970353 1104 1 0.36429373704558543 1104 2 0.4622990919519907 1104 3 0.44386239397498173 1104 4 0.3623949044340051 1104 5 0.5458763212480449 1104 6 0.5317602071235115 1104 7 0.5321471250376462 1104 8 0.4437950244068034 1104 9 0.5088155619021748 1104 10-11 0.3428229938121774 1104 12-13 0.44024086303183196 1104 14-15 0.48630024204857136 1104 16-17 0.518962152873776 1104 18-19 0.5685945013424814 1104 20-21 0.5390469694208093 1104 22-23 0.5220666806421832 1104 24-25 0.567907091176302 1104 26-27 0.2583362949833514 1104 28-29 0.27561329910197685 1104 30-31 0.4034594693733098 1104 32-33 0.29166616375465537 1104 34-35 0.40473304582905456 1104 36-37 0.22314292721041085 1104 38-39 0.36692331445466664 1104 40-41 0.4608258841951596 1104 42-43 0.4646520617902823 1104 44-45 0.47494715389087716 1104 46-47 0.35367601099906665 1104 48-49 0.4234489450151244 1104 50-51 0.33143138111862314 1104 52-53 0.31742728260366704 1104 54-55 0.36347295020905435 1104 56-57 0.4910951515998754 1104 58-59 0.4123423166929072 1104 60-61 0.2627973140591422 1104 62-63 0.324409313596405 1104 64-65 0.29746769529542405 1104 66-67 0.26475379361274776 1104 68-69 0.24248587549031342 1104 70-71 0.32117720962316554 1104 72-73 0.14732753743055227 1104 74-75 0.12024794206899614 1104 76-77 0.12339941874598281 1105 1 0.385396628780974 1105 2 0.49947338185707935 1105 3 0.31154501268949986 1105 4 0.45491141119757117 1105 5 0.4952624832453978 1105 6 0.21691932830498217 1105 7 0.05328520756593491 1105 8 0.4575559407926022 1105 9 0.3309739317031699 1105 10-11 0.3575617052464466 1105 12-13 0.4359592580578777 1105 14-15 0.3285923087801379 1105 16-17 0.298081568660983 1105 18-19 0.3644441992720928 1105 20-21 0.5944179707682409 1105 22-23 0.5321053589216334 1105 24-25 0.6523362973931128 1105 26-27 0.4115765771906936 1105 28-29 0.5850435715734932 1105 30-31 0.484396450122631 1105 32-33 0.5616356223587857 1105 34-35 0.7829257765871986 1105 36-37 0.7584702709128308 1105 38-39 0.7233022292548625 1105 40-41 0.7736251704802442 1105 42-43 0.5922373784012294 1105 44-45 0.47915864055181245 1105 46-47 0.45619609300677766 1105 48-49 0.48870610195359987 1105 50-51 0.5120762519596376 1105 52-53 0.2904607798506227 1105 54-55 0.29144345242642444 1105 56-57 0.47105675941016045 1105 58-59 0.3587339265058205 1105 60-61 0.30536395673288297 1105 62-63 0.27264690427851335 1105 64-65 0.36951351598947113 1105 66-67 0.5552867858147863 1105 68-69 0.32820108140559867 1105 70-71 0.018366141004278802 1105 72-73 -0.19153619135778044 1105 74-75 -0.11406657375571783 1105 76-77 0.19861328837646752 1106 1 0.14222585540559152 1106 2 0.2702111876699931 1106 3 -0.23939858851075257 1106 4 0.04704317631573218 1106 5 -0.04836710257934129 1106 6 0.13267796334451276 1106 7 0.1868468520016222 1106 8 0.30527226030548604 1106 9 0.15353380204069111 1106 10-11 0.07068705294778255 1106 12-13 0.0634625105711848 1106 14-15 0.24956486501466912 1106 16-17 0.2201844663546595 1106 18-19 0.26651170261221324 1106 20-21 0.015997312380704898 1106 22-23 0.13709946716352306 1106 24-25 0.21282200604677115 1106 26-27 0.1441645264646283 1106 28-29 0.1340638751414005 1106 30-31 0.07040639383333769 1106 32-33 0.1363628273875932 1106 34-35 0.1973013159349435 1106 36-37 0.2828740644165677 1106 38-39 0.03723271088966129 1106 40-41 0.21564992024479324 1106 42-43 0.18389350999112963 1106 44-45 -0.0056254670330062595 1106 46-47 0.09903684031825222 1106 48-49 0.051570679126871966 1106 50-51 -0.07643232284817003 1106 52-53 -0.00398327034978152 1106 54-55 0.0980999563579168 1106 56-57 0.06724503284030448 1106 58-59 0.0886938450287218 1106 60-61 0.017865084708880374 1106 62-63 -0.0832425321715391 1106 64-65 -0.12489408853995698 1106 66-67 -0.1727382705840128 1106 68-69 -0.2802786697826072 1106 70-71 -0.42501515868689665 1106 72-73 -0.33650349100514987 1106 74-75 -0.5122287832745194 1106 76-77 -0.5550880060581207 1107 1 -0.06802473719788082 1107 2 0.005661922917830964 1107 3 0.04701235315189223 1107 4 0.10666265146834064 1107 5 0.1930183938440635 1107 6 -0.18278795933384373 1107 7 -0.20780549428857853 1107 8 0.0716511489379883 1107 9 -0.08859344513264489 1107 10-11 -0.02162047154299529 1107 12-13 0.10075251917179884 1107 14-15 0.07667291812872179 1107 16-17 0.016432583658676947 1107 18-19 0.1820224324060078 1107 20-21 0.05065267937530393 1107 22-23 -0.05545466427906831 1107 24-25 -0.3898586173930525 1107 26-27 -0.22580052566350162 1107 28-29 -0.07530576258383803 1107 30-31 0.08010898031149338 1107 32-33 0.13904873706014342 1107 34-35 0.10643198237785612 1107 36-37 0.13564593830215443 1107 38-39 0.12637973948146453 1107 40-41 0.17830075618277874 1107 42-43 0.1386640832956516 1107 44-45 0.041440128402374654 1107 46-47 0.003338407308664415 1107 48-49 -0.049159827889976526 1107 50-51 -0.057202434656186796 1107 52-53 -0.056059039685440126 1107 54-55 -0.07425111662146477 1107 56-57 -0.11142342387342197 1107 58-59 -0.03781577297803551 1107 60-61 0.003676332637386537 1107 62-63 0.04711831614294937 1107 64-65 0.015575043636840036 1107 66-67 0.09625797451258578 1107 68-69 -0.028646021002426636 1107 70-71 0.00316717438771974 1107 72-73 0.0018891586141158712 1107 74-75 -0.006221441284633755 1107 76-77 0.037782292446213006 1108 1 -0.03207613458136649 1108 2 -0.03253995878127114 1108 3 -0.20477601288704506 1108 4 -0.24160253869121107 1108 5 -0.06733163621559157 1108 6 -0.1647289111558834 1108 7 -0.23709222831307386 1108 8 0.10737466259418227 1108 9 -0.09440561484685617 1108 10-11 -0.20781595517203044 1108 12-13 -0.1549994356613098 1108 14-15 0.037679720498701386 1108 16-17 -0.12739153600134756 1108 18-19 -0.12300329591527515 1108 20-21 -0.23131919231028775 1108 22-23 -0.2011971376578856 1108 24-25 -0.22978946267298994 1108 26-27 -0.3308115347916285 1108 28-29 -0.21789834455652368 1108 30-31 -0.24058910450483495 1108 32-33 -0.10284328140958365 1108 34-35 -0.06441133499694018 1108 36-37 -0.0733848442459859 1108 38-39 -0.18823146513793887 1108 40-41 -0.13328600469946394 1108 42-43 -0.012017045774783242 1108 44-45 -0.18728872589959522 1108 46-47 -0.23061775179752786 1108 48-49 -0.137464871380093 1108 50-51 -0.10852614252926784 1108 52-53 -0.11241467259640459 1108 54-55 -0.14568699302994403 1108 56-57 -0.23206314167271813 1108 58-59 -0.24214716637268197 1108 60-61 -0.30340371078104766 1108 62-63 -0.18180376739388748 1108 64-65 -0.28199973034230297 1108 66-67 -0.3503759499142376 1108 68-69 -0.4212450545695141 1108 70-71 -0.4514647358672548 1108 72-73 -0.45908020857845955 1108 74-75 -0.6635071710905827 1108 76-77 -0.4540286430222338 1109 1 0.1734494812309464 1109 2 0.06389777606514002 1109 3 -0.09389900251470351 1109 4 0.2627863664815493 1109 5 0.05944648948877074 1109 6 0.12427780198913041 1109 7 0.04792767831104072 1109 8 0.35151093888980967 1109 9 0.27588177766753574 1109 10-11 0.09263452752369261 1109 12-13 0.1264401968198854 1109 14-15 0.32931918020968 1109 16-17 0.356146845072729 1109 18-19 0.1187923760586358 1109 20-21 0.2655256875744314 1109 22-23 0.3116904949548669 1109 24-25 0.3283624828943985 1109 26-27 0.24027874909260305 1109 28-29 0.34747420613224733 1109 30-31 0.42119707768294745 1109 32-33 0.2872989009316811 1109 34-35 0.4228614526623957 1109 36-37 0.47334832186332676 1109 38-39 0.46256270388344234 1109 40-41 0.5338760300938361 1109 42-43 0.5329690710195578 1109 44-45 0.3978114875964067 1109 46-47 0.22389108969724703 1109 48-49 0.31102708132639734 1109 50-51 0.4529481149344683 1109 52-53 0.5179308291385496 1109 54-55 0.4429230386703935 1109 56-57 0.4554401030086659 1109 58-59 0.41155238306400577 1109 60-61 0.2766015871185914 1109 62-63 0.34753371461727767 1109 64-65 0.4786095313407266 1109 66-67 0.3986067114758143 1109 68-69 0.42813473969914284 1109 70-71 0.442159211284018 1109 72-73 0.4929650128482699 1109 74-75 0.37193410074067756 1109 76-77 0.46426020310941496 1110 1 -0.25221755040623606 1110 2 -0.27811801371183975 1110 3 -0.3913807059665544 1110 4 -0.2941029321987969 1110 5 -0.09600213610911368 1110 6 -0.3140556273890738 1110 7 -0.4518591020727527 1110 8 -0.17301507966606522 1110 9 -0.3130884292131242 1110 10-11 -0.31906950059823913 1110 12-13 -0.2749947135702513 1110 14-15 -0.44029126178882905 1110 16-17 -0.3348258973925482 1110 18-19 -0.2509823026712681 1110 20-21 -0.46782589428461563 1110 22-23 -0.7243205694144024 1110 24-25 -0.49021355583089843 1110 26-27 -0.31734817420596784 1110 28-29 -0.3258243589573624 1110 30-31 -0.32701648769480585 1110 32-33 -0.27113768063923516 1110 34-35 -0.06519248613781059 1110 36-37 -0.13321250335421553 1110 38-39 -0.1808879962836798 1110 40-41 -0.21134093527237496 1110 42-43 -0.15784231606048493 1110 44-45 -0.23464111425917977 1110 46-47 -0.24354393594673596 1110 48-49 -0.1522443991092608 1110 50-51 -0.2363880229091322 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-0.5594214161439339 2314 24-25 -0.5736812393320889 2314 26-27 -0.5961646678347918 2314 28-29 -0.6302821104660481 2314 30-31 -0.7749207544663648 2314 32-33 -0.9356080347543596 2314 34-35 -0.9554101981048504 2314 36-37 -1.0082101743304364 2314 38-39 -1.0168993619197124 2314 40-41 -0.8739643735325444 2314 42-43 -0.7017233236479257 2314 44-45 -0.6495639459380449 2314 46-47 -0.7193637523161485 2314 48-49 -0.7844322840451348 2314 50-51 -0.7287286400486792 2314 52-53 -0.5479035469125044 2314 54-55 -0.6045571623100692 2314 56-57 -0.3714879289206685 2314 58-59 -0.551357304964224 2314 60-61 -0.6119269993887677 2314 62-63 -0.54729234168191 2314 64-65 -0.5778642451647613 2314 66-67 -0.5169368031083579 2314 68-69 -0.5003368749469956 2314 70-71 -0.3781621179080048 2314 72-73 -0.369470261147665 2314 74-75 -0.4441415108037994 2314 76-77 -0.5778723294671799 2315 1 -1.3766800968258224 2315 2 -1.3651242152765164 2315 3 -1.407023761227748 2315 4 -1.2964628441052497 2315 5 -1.3044825223937906 2315 6 -1.4309933629425657 2315 7 -1.4684955134585707 2315 8 -1.360096842642811 2315 9 -1.427114285959739 2315 10-11 -1.2639317853173324 2315 12-13 -1.3835663264378084 2315 14-15 -1.6655531926593454 2315 16-17 -1.4948903088889338 2315 18-19 -1.3109670060927243 2315 20-21 -1.5022528715516899 2315 22-23 -1.5553274713043663 2315 24-25 -1.572462613616949 2315 26-27 -1.4866149520646346 2315 28-29 -1.4156664171765065 2315 30-31 -1.5041089398469154 2315 32-33 -1.4766707111125967 2315 34-35 -1.4225343830978403 2315 36-37 -1.5819086082555671 2315 38-39 -1.4254848945798635 2315 40-41 -1.4409485688179586 2315 42-43 -1.5789446833442362 2315 44-45 -1.4614069015261606 2315 46-47 -1.4596237730289587 2315 48-49 -1.459990826068097 2315 50-51 -1.3855034014994523 2315 52-53 -1.4143094677047436 2315 54-55 -1.519036727834667 2315 56-57 -1.577771709492854 2315 58-59 -1.4739343244403003 2315 60-61 -1.5439954855712372 2315 62-63 -1.588845130552933 2315 64-65 -1.7094698238208785 2315 66-67 -1.6489465717665652 2315 68-69 -1.510410203868421 2315 70-71 -1.593864011128261 2315 72-73 -2.8664352655489083 2315 74-75 -3.4466894217442032 2315 76-77 -3.325636573179981 2316 1 -2.9481574125259584 2316 2 -2.8183721692619272 2316 3 -2.2569501075851477 2316 4 -2.344834326819509 2316 5 -2.8966485055487183 2316 6 -2.1477647730121348 2316 7 -2.6956932183895788 2316 8 -2.5829201422997983 2316 9 -2.1695525687671946 2316 10-11 -2.1634299420762844 2316 12-13 -2.225979699874223 2316 14-15 -2.362128488276319 2316 16-17 -3.7556735376099013 2316 18-19 -3.3407228942565936 2316 20-21 -2.6490888966083332 2316 22-23 -2.7425506804961692 2316 24-25 -2.7041965626655546 2316 26-27 -2.447849956321896 2316 28-29 -2.6793302751073966 2316 30-31 -2.7888679295616186 2316 32-33 -2.9697474521544613 2316 34-35 -2.993444588025575 2316 36-37 -2.8788463935307647 2316 38-39 -2.967195380861625 2316 40-41 -2.936864663678506 2316 42-43 -3.2360263087717627 2316 44-45 -3.036741369779879 2316 46-47 -2.841982618397715 2316 48-49 -3.0616311494260664 2316 50-51 -3.304687430255427 2316 52-53 -4.293532038503837 2316 54-55 -3.5440749698122396 2316 56-57 -3.477368665124825 2316 58-59 -3.425536504095433 2316 60-61 -3.3246300809531633 2316 62-63 -3.266433680680784 2316 64-65 -3.1773818105351843 2316 66-67 -3.2527888389727053 2316 68-69 -3.369274179309695 2316 70-71 -3.2383239494955838 2316 72-73 -3.2662771873520917 2316 74-75 -3.1579076537789916 2316 76-77 -3.1463607112646415 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 68434.0 3 6631.0 4 8550.0 5 10138.0 6 11596.0 7 11883.0 8 14021.0 9 15443.0 10 19402.0 11 21892.0 12 16518.0 13 13138.0 14 13026.0 15 13520.0 16 13267.0 17 13867.0 18 14506.0 19 15573.0 20 16505.0 21 17792.0 22 19711.0 23 22486.0 24 25990.0 25 30327.0 26 35828.0 27 42483.0 28 49566.0 29 58611.0 30 68764.0 31 83528.0 32 101707.0 33 128654.0 34 172402.0 35 231730.0 36 316893.0 37 399760.0 38 319631.0 39 74042.0 40 447.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.3256116241046 25.643236382395774 28.353234638954827 22.677917354544803 2 23.26259054664977 25.96210097219867 28.01762261502736 22.757685866124206 3 23.23646969860646 26.046187622721124 27.842403840654768 22.874938838017652 4 22.877847448714547 25.93209810839965 28.392791043851386 22.79726339903442 5 23.177750630279135 25.764913428956778 28.112276264807782 22.9450596759563 6 23.09064582259398 25.666620863082496 28.382429215591117 22.860304098732406 7 23.010978431564123 25.598745912180494 28.158632418344887 23.2316432379105 8 23.13429871873287 25.965200054014982 27.780222573496122 23.120278653756028 9 23.07574378916339 25.758424857723973 28.09830897162105 23.067522381491585 10-11 23.614117262028525 25.826684055176948 27.60694102621999 22.95225765657454 12-13 23.697150311388366 25.507514089973675 27.528356189927344 23.266979408710615 14-15 23.241516252655824 25.660987669029605 27.758861023410976 23.338635054903595 16-17 23.427125097692862 25.53746958019075 27.92024211944263 23.115163202673767 18-19 23.607618741984542 25.72580488213676 27.305602411002344 23.36097396487635 20-21 23.347581405759062 25.935093056005986 27.28452579101031 23.432799747224646 22-23 23.58187898390857 25.99316083904141 26.87894716878238 23.546013008267643 24-25 23.787398982186073 26.05194163516697 26.60499517624672 23.55566420640024 26-27 23.64816396242528 26.19976962643735 26.457192222906283 23.69487418823109 28-29 23.89157749009348 26.115014944174792 26.499361804706677 23.49404576102505 30-31 23.580212348120657 26.05190158198754 26.507097796496076 23.860788273395723 32-33 23.528750639151305 26.382279499004664 26.53098953032928 23.557980331514752 34-35 23.727080579772768 25.750510732531833 26.698344527616097 23.824064160079303 36-37 23.708665213647787 25.96116881177581 26.90526041550833 23.424905559068076 38-39 23.49025327679145 26.0381330058114 26.862848864975643 23.608764852421515 40-41 23.39045362497672 26.045558291014036 26.73434973774415 23.829638346265092 42-43 23.419630532730363 26.345895347592617 26.48174438021684 23.752729739460182 44-45 23.35067646555954 25.998454666749538 26.903016735947187 23.74785213174373 46-47 23.287416047469797 26.148762950108495 26.74803377603576 23.815787226385947 48-49 23.519486637269164 26.06115803714063 26.613155634923345 23.806199690666862 50-51 23.30595918944568 26.444491584168837 26.390267659619944 23.859281566765542 52-53 23.472855049022655 26.195742226302503 26.56972109891948 23.76168162575536 54-55 23.46330713440499 26.13729393020866 26.63671141550081 23.76268751988554 56-57 23.2789698752579 26.67445018090812 26.456335696521588 23.590244247312395 58-59 23.456427770429098 26.415535718860223 26.32601441753664 23.802022093174042 60-61 23.30699610641177 26.218508822832003 26.287400272229455 24.18709479852677 62-63 23.494662276505853 26.066993578605334 26.390674926651357 24.047669218237452 64-65 23.360977407830795 26.119956402888782 26.378516347725096 24.140549841555323 66-67 23.294113721647342 26.341422336867083 26.543088015132277 23.8213759263533 68-69 23.330631210256495 25.992682616277534 26.63032671985234 24.04635945361363 70-71 23.23739152063025 25.92504960589438 26.595374831435315 24.24218404204005 72-73 23.461284937194673 25.811876743419347 26.504266477641607 24.222571841744372 74-75 23.2607006937623 25.899962381712545 26.431030059455974 24.408306865069186 76-77 23.09345915489375 25.75736979837389 26.78813598394877 24.361035062783593 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 10170.0 1 6157.0 2 2003.0 3 1879.5 4 1897.0 5 1954.5 6 2095.0 7 2310.0 8 2460.0 9 2478.0 10 2583.0 11 2730.5 12 2952.5 13 3132.0 14 3256.0 15 3648.0 16 4109.5 17 4303.0 18 4596.0 19 5240.0 20 6139.5 21 7249.0 22 7810.0 23 8846.0 24 10991.5 25 13745.5 26 15390.0 27 16805.0 28 21362.5 29 26380.0 30 28255.0 31 32028.0 32 39437.5 33 45541.5 34 51735.5 35 55462.0 36 60345.0 37 69027.0 38 75091.0 39 77356.0 40 81079.0 41 88874.0 42 99592.0 43 106238.0 44 107113.5 45 108316.5 46 113675.5 47 119182.5 48 119658.0 49 121872.0 50 125721.0 51 125557.5 52 123759.0 53 124170.0 54 118332.0 55 106103.0 56 93220.5 57 86318.0 58 85085.0 59 76778.5 60 65367.5 61 61030.0 62 54833.5 63 41293.0 64 30173.5 65 26398.0 66 23310.5 67 18380.0 68 14541.0 69 10663.5 70 8782.0 71 7571.5 72 5510.5 73 4310.5 74 3961.0 75 3399.0 76 2235.0 77 1270.5 78 908.0 79 765.0 80 527.5 81 350.0 82 231.5 83 196.0 84 177.0 85 136.0 86 105.0 87 96.0 88 94.0 89 83.0 90 68.0 91 62.0 92 49.5 93 39.5 94 37.5 95 37.5 96 42.0 97 35.0 98 36.0 99 98.0 100 152.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010046611512225495 2 0.014335283620211083 3 0.014216153839433705 4 0.015645711208762233 5 0.013104275885511517 6 0.010483420708409212 7 0.016519329601129666 8 0.01755178770120027 9 0.01767091748197765 10-11 0.020152787914839678 12-13 0.016896573906924697 14-15 0.008418504508268004 16-17 0.00659184786968155 18-19 0.018703375582048255 20-21 0.024282620315122096 22-23 0.022058864407277716 24-25 0.020371192512931537 26-27 0.024302475278584992 28-29 0.008895023631377515 30-31 0.00901415341215489 32-33 0.010900374941130034 34-35 0.012310077346995666 36-37 0.011357039100776647 38-39 0.014474268364451356 40-41 0.011952688004663535 42-43 0.015625856245299337 44-45 0.014236008802896601 46-47 0.02458044476706554 48-49 0.02334943703236597 50-51 0.03665226255250645 52-53 0.04000775137773591 54-55 0.028948536728902713 56-57 0.02174118499187138 58-59 0.016896573906924697 60-61 0.021165391051447387 62-63 0.026307826588337514 64-65 0.0263673914787262 66-67 0.028948536728902713 68-69 0.04389932421646358 70-71 0.056546935942328486 72-73 0.08710372471172578 74-75 0.08730227434635475 76-77 0.09204761061398695 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2518262.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.85941971682302 #Duplication Level Percentage of deduplicated Percentage of total 1 74.19019625270984 35.506997413319105 2 12.705516570652891 12.161573005478532 3 5.043125103804446 7.24083123082271 4 2.4864915862347514 4.76008177791832 5 1.4501543707471931 3.4701773341887647 6 0.8966921620511732 2.5749099924235543 7 0.6157084008994135 2.0627212747273274 8 0.4269512312372005 1.634691053951643 9 0.32677189677783336 1.4075202023597424 >10 1.628176336338635 14.005339117047031 >50 0.13527095155628494 4.5021400760264685 >100 0.08918425736915596 8.010794956488137 >500 0.004925969531109443 1.6061643261952068 >1k 7.514190810162546E-4 0.6583531554975746 >5k 8.349100900180607E-5 0.3977050835559213 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9953 0.39523290269241246 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3578 0.14208211854048544 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.9854963462896235E-4 0.0 3 0.0 0.0 0.0 4.368091961837172E-4 0.0 4 0.0 0.0 0.0 0.0012310077346995667 0.0 5 0.0 0.0 0.0 0.0030576643732860205 0.0 6 0.0 0.0 0.0 0.005877069185017286 0.0 7 0.0 0.0 0.0 0.010523130635335005 0.0 8 0.0 0.0 0.0 0.015804550916465404 0.0 9 0.0 0.0 0.0 0.022396398786146954 0.0 10 0.0 0.0 0.0 0.028472017605793203 0.0 11 0.0 0.0 0.0 0.03629487321017432 0.0 12 0.0 0.0 0.0 0.04796959172635731 0.0 13 0.0 0.0 0.0 0.062463715054271556 0.0 14 0.0 0.0 0.0 0.07890362480154964 3.970992692579247E-5 15 0.0 0.0 0.0 0.09899684782600063 3.970992692579247E-5 16 0.0 0.0 0.0 0.11845471201963895 3.970992692579247E-5 17 0.0 0.0 0.0 0.1375948967978709 3.970992692579247E-5 18 0.0 0.0 0.0 0.15733073047998977 3.970992692579247E-5 19 0.0 0.0 0.0 0.17869467116606613 1.1912978077737741E-4 20 0.0 0.0 0.0 0.20271917695617056 1.1912978077737741E-4 21 0.0 0.0 0.0 0.23242220229666333 1.5883970770316989E-4 22 0.0 0.0 0.0 0.2623237772717851 1.9854963462896235E-4 23 0.0 0.0 0.0 0.29524330669326704 2.3825956155475481E-4 24 0.0 0.0 0.0 0.3241918434221697 3.5738934233213226E-4 25 0.0 0.0 0.0 0.35786586145524174 3.5738934233213226E-4 26 0.0 0.0 0.0 0.39372392546923235 3.5738934233213226E-4 27 0.0 0.0 0.0 0.4266434548907143 3.5738934233213226E-4 28 0.0 0.0 0.0 0.46277948839318545 3.5738934233213226E-4 29 0.0 0.0 0.0 0.49835958291869553 3.5738934233213226E-4 30 0.0 0.0 0.0 0.5349721355442761 3.5738934233213226E-4 31 0.0 0.0 0.0 0.5796060934088669 3.5738934233213226E-4 32 0.0 0.0 0.0 0.6270197461582632 3.5738934233213226E-4 33 0.0 0.0 0.0 0.6772130937924647 3.5738934233213226E-4 34 0.0 0.0 0.0 0.7322113425846873 3.5738934233213226E-4 35 0.0 0.0 0.0 0.7874478509384647 3.5738934233213226E-4 36 0.0 0.0 0.0 0.8445507258577543 3.5738934233213226E-4 37 0.0 0.0 0.0 0.905267204127291 3.5738934233213226E-4 38 0.0 0.0 0.0 0.9630054378773932 3.5738934233213226E-4 39 0.0 0.0 0.0 1.0228085878276367 3.5738934233213226E-4 40 0.0 0.0 0.0 1.0848354936857245 3.5738934233213226E-4 41 0.0 0.0 0.0 1.1551220643443771 3.5738934233213226E-4 42 0.0 0.0 0.0 1.2337080097305204 3.5738934233213226E-4 43 0.0 0.0 0.0 1.3149942301476176 3.5738934233213226E-4 44 0.0 0.0 0.0 1.402395779311287 3.5738934233213226E-4 45 0.0 0.0 0.0 1.4924181836520585 3.5738934233213226E-4 46 0.0 0.0 0.0 1.5904222833049142 4.368091961837172E-4 47 0.0 0.0 0.0 1.6898956502540243 4.368091961837172E-4 48 0.0 0.0 0.0 1.793141460261085 4.7651912310950963E-4 49 0.0 0.0 0.0 1.9000008736183924 4.7651912310950963E-4 50 3.970992692579247E-5 0.0 0.0 2.0110298293029083 4.7651912310950963E-4 51 3.970992692579247E-5 0.0 0.0 2.127975564099367 4.7651912310950963E-4 52 3.970992692579247E-5 0.0 0.0 2.2539354523079806 4.7651912310950963E-4 53 3.970992692579247E-5 0.0 0.0 2.383628073647619 5.162290500353022E-4 54 3.970992692579247E-5 0.0 0.0 2.5248762837226626 5.162290500353022E-4 55 3.970992692579247E-5 0.0 0.0 2.6663230434323353 5.162290500353022E-4 56 3.970992692579247E-5 0.0 0.0 2.829292583535788 5.162290500353022E-4 57 3.970992692579247E-5 0.0 0.0 2.989204459265954 5.162290500353022E-4 58 3.970992692579247E-5 0.0 0.0 3.158329038042904 6.353588308126795E-4 59 3.970992692579247E-5 0.0 0.0 3.325666670108194 6.353588308126795E-4 60 3.970992692579247E-5 0.0 0.0 3.501422806681751 6.353588308126795E-4 61 3.970992692579247E-5 0.0 0.0 3.686351936375167 6.353588308126795E-4 62 3.970992692579247E-5 0.0 0.0 3.8777140742305605 6.353588308126795E-4 63 3.970992692579247E-5 0.0 0.0 4.075509220247933 6.353588308126795E-4 64 3.970992692579247E-5 0.0 0.0 4.283390687704457 6.353588308126795E-4 65 3.970992692579247E-5 0.0 0.0 4.5044955608272685 6.353588308126795E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 595 0.0 28.635885 59 ATTCGCG 610 0.0 27.351984 56 CGATCGT 650 0.0 27.30288 1 TCGCGGA 675 0.0 26.293753 58 TCGCGCT 670 0.0 25.960691 37 CTCGCGC 680 0.0 25.578405 36 AATTCGC 675 0.0 24.717108 55 ATCGTTC 750 0.0 24.135267 3 GATCGTT 740 0.0 23.981783 2 CGGTTAC 575 0.0 23.455803 4 CGCGCTT 745 0.0 23.34627 38 CAATTCG 720 0.0 23.17551 54 CCTCGCG 800 0.0 23.07185 35 CGCTCCG 765 0.0 22.735458 25 AACTCGA 810 0.0 22.349243 13 ACTCGAG 840 0.0 21.973627 14 GCCTAGT 750 0.0 19.40633 17 GACGCTC 940 0.0 19.25992 23 GCCCTCG 970 0.0 19.027952 33 GCGCCCT 920 0.0 18.904654 31 >>END_MODULE