FastQCFastQC Report
Thu 26 May 2016
SRR936021_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936021_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2518262
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100780.40019664355813656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74370.2953227265471186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53130.2109788417567354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA15500.051.64802100-101
CGTACAT17800.041.463387100-101
CGGTATC14100.039.680016100-101
GGTATCA100950.038.8453181
ACGTATC4900.035.833485100-101
AGGTATC21800.035.630108100-101
TACGCAG10700.035.32263100-101
CCGTACA8050.035.12051100-101
AGTATCA27600.034.612186100-101
TCGTATC6950.033.827927100-101
GCGTATC5000.033.33121100-101
GGGTATC12700.033.274975100-101
TACCGTA2300.032.34687498-99
GTATCAA195350.030.7048321
GGTACAT24850.030.658201100-101
CGAGTAC13900.029.33181100-101
ACGTACA7650.029.176481100-101
CGGGTAT2450.029.152664100-101
CCGGTAT5350.028.925621100-101
CACGTAT3300.028.85733298-99
Produced by FastQC (version 0.11.5)