FastQCFastQC Report
Thu 26 May 2016
SRR936020_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936020_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525969
Sequences flagged as poor quality0
Sequence length77
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100060.3961252097709829No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34030.13472057653914202No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC5050.030.922574
CGCGGAA5350.029.8620259
ATTCGCG5850.027.91438356
TCGCGCT6100.027.34906437
AATTCGC6350.026.27337355
CTCGCGC6300.025.91433736
CAATTCG6800.024.53420454
CGATCGT6950.024.511061
TCGCGGA6850.023.84164858
CGCGCTT7000.023.83511538
GCCTAGT6600.022.58917
ACTCGAG7800.021.84257914
CGCTCCG7400.021.1054925
CCTCGCG8400.020.70207435
GATCGTT8500.020.0413972
ATCGTTC8600.019.8083573
GACGCTC8500.019.6289223
AACTCGA8900.019.14293313
GACGTAT7800.018.66015839
CGTCGTT2559.385985E-1018.0929263
Produced by FastQC (version 0.11.5)