FastQCFastQC Report
Thu 26 May 2016
SRR936020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525969
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103560.40998127847174687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74350.2943424879719427No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51770.20495105046815695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13950.050.995525100-101
CGTACAT17150.044.95148100-101
GGTATCA98050.039.877031
CGGTATC12200.038.304356100-101
CCGTACA7600.037.206463100-101
ACGTATC4200.036.852116100-101
TACGCAG10350.034.797928100-101
GGGTATC13700.034.76224100-101
TCGTACA8650.033.72239100-101
AGTATCA30850.033.672752100-101
AGGTATC22450.033.278656100-101
GCGTATC4350.032.844326100-101
GTATCAA194750.031.7499581
TCGAGTA4700.031.665075100-101
CCCGTAC5050.030.05570898-99
GGTACAT21700.029.902338100-101
GCGTACA5850.029.510767100-101
CGAGTAC14250.029.242975100-101
CCGTATC7600.028.981874100-101
AACGTAT3300.028.85919698-99
Produced by FastQC (version 0.11.5)