FastQCFastQC Report
Thu 26 May 2016
SRR936019_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936019_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561906
Sequences flagged as poor quality0
Sequence length77
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61020.23818204102726642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGT4550.026.5226571
CGCGGAA3900.026.39625259
GCGTTCG1259.611722E-825.55685810
CTCGCGC4600.025.46227336
TCGCGCT4900.025.35452337
ATTCGCG4100.025.1096156
AATTCGC4400.024.2034655
CCTCGCG5300.024.1083635
CGCGCTT5250.022.988138
CGTTCGG1402.873112E-722.81862411
CAATTCG4700.022.66165754
CGCTCCG5600.022.1856425
CGGTTAC2800.021.5483994
TCGCGGA4850.020.49352658
GATCGTT6050.019.9467932
ATCGTTC6250.019.8752293
GCGCCCT6350.018.4454731
GACGCTC7150.017.87367423
ACGCTCC7450.017.62973624
AAACTCG7000.017.24208512
Produced by FastQC (version 0.11.5)