##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936019_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2561906 Sequences flagged as poor quality 0 Sequence length 77 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.24482748391237 39.0 36.0 41.0 29.0 41.0 2 36.09880924592862 39.0 36.0 41.0 28.0 41.0 3 36.00390724718237 39.0 35.0 41.0 28.0 41.0 4 35.873914967996484 39.0 35.0 41.0 27.0 41.0 5 35.79916163981036 39.0 35.0 40.0 27.0 41.0 6 35.69741044363064 39.0 35.0 40.0 27.0 41.0 7 35.517713764673644 39.0 35.0 40.0 27.0 41.0 8 35.334676994394016 38.0 35.0 40.0 26.0 41.0 9 35.23416198720796 38.0 34.0 40.0 26.0 41.0 10-11 35.438839871564376 39.0 35.0 40.0 27.0 41.0 12-13 35.618549041221655 39.0 35.0 41.0 27.0 41.0 14-15 35.55022569134074 39.0 35.0 41.0 27.0 41.0 16-17 35.36816787969582 39.0 35.0 41.0 26.0 41.0 18-19 35.19689032306415 39.0 35.0 41.0 25.5 41.0 20-21 34.9753544821707 39.0 34.0 41.0 24.5 41.0 22-23 34.68658510499605 38.5 34.0 41.0 24.0 41.0 24-25 34.4250335492403 38.0 34.0 40.0 23.0 41.0 26-27 33.46230775055759 37.5 32.0 39.5 21.0 40.5 28-29 33.47287624916761 37.5 33.0 39.5 20.5 40.5 30-31 33.81127527707886 38.0 33.0 40.0 20.5 41.0 32-33 33.62622769922081 38.0 33.0 40.0 19.5 41.0 34-35 33.32137381309072 37.0 33.0 40.0 18.0 41.0 36-37 32.997717910024804 37.0 32.0 40.0 15.0 41.0 38-39 32.68558526347181 36.0 32.0 40.0 13.0 41.0 40-41 32.281533943868354 36.0 31.5 39.0 9.0 41.0 42-43 31.89659359086555 35.0 31.0 39.0 7.5 41.0 44-45 31.51716456419556 35.0 31.0 38.5 6.5 40.0 46-47 31.11179547571222 35.0 31.0 37.5 2.0 40.0 48-49 30.648189668161127 35.0 30.0 37.0 2.0 39.0 50-51 30.2097668688859 35.0 30.0 36.5 2.0 39.0 52-53 29.77118988752905 34.0 29.5 36.0 2.0 38.5 54-55 29.322352966892616 34.0 29.0 35.5 2.0 37.5 56-57 28.887106318498805 34.0 29.0 35.0 2.0 37.0 58-59 28.479078467359848 34.0 28.5 35.0 2.0 36.5 60-61 28.14149113979982 34.0 27.5 35.0 2.0 36.0 62-63 27.801740188750095 34.0 27.0 35.0 2.0 36.0 64-65 27.431190098309617 33.5 26.5 35.0 2.0 35.0 66-67 27.043019142778853 33.0 25.5 35.0 2.0 35.0 68-69 26.669778672597666 33.0 25.0 35.0 2.0 35.0 70-71 26.23568546230814 33.0 24.5 35.0 2.0 35.0 72-73 25.730713968428194 33.0 23.5 35.0 2.0 35.0 74-75 25.121334467384827 32.5 19.0 35.0 2.0 35.0 76-77 23.416330068316324 30.5 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9390661242240128 1101 2 0.8034865861023448 1101 3 0.9626791261400882 1101 4 0.9310495226950337 1101 5 1.0750154430816465 1101 6 0.900775434531262 1101 7 0.965972585277612 1101 8 1.041673338636393 1101 9 1.045542399263482 1101 10-11 0.9049007518285492 1101 12-13 0.9891201918340897 1101 14-15 1.0541232339694275 1101 16-17 1.1839545157986535 1101 18-19 1.2135119880313638 1101 20-21 1.128287084604267 1101 22-23 1.1828442323775121 1101 24-25 1.251016123694626 1101 26-27 1.0852126930164232 1101 28-29 1.185825736975687 1101 30-31 1.1729973836228567 1101 32-33 1.340934111471448 1101 34-35 1.2482817951503336 1101 36-37 1.1868635062879278 1101 38-39 1.2202470012623863 1101 40-41 1.1452110495352343 1101 42-43 1.1830369933243077 1101 44-45 1.1341358938632986 1101 46-47 1.126891992345584 1101 48-49 1.2071987068294838 1101 50-51 1.2075941108553785 1101 52-53 1.1427827575208873 1101 54-55 1.189339862407266 1101 56-57 1.1788508013388856 1101 58-59 1.1400531521989095 1101 60-61 1.03685150325175 1101 62-63 1.0224290725887961 1101 64-65 1.0708114932128439 1101 66-67 0.9302161083864284 1101 68-69 0.928528771332882 1101 70-71 1.0869518497359714 1101 72-73 1.0963360019240085 1101 74-75 1.0288302307296284 1101 76-77 1.3091333021909968 1102 1 0.6625370381250306 1102 2 0.8909203808131423 1102 3 0.7352372217346996 1102 4 0.7856943927293898 1102 5 0.8493899050448022 1102 6 0.9190832823591393 1102 7 0.6899324868714913 1102 8 0.852099497425769 1102 9 0.7466062128650535 1102 10-11 0.8059849458144299 1102 12-13 0.9644706300654278 1102 14-15 0.7986408752088465 1102 16-17 0.7988021388124977 1102 18-19 0.8247551843974321 1102 20-21 0.8512935132709742 1102 22-23 0.8335647815722567 1102 24-25 0.8318827287719657 1102 26-27 0.7581436151898941 1102 28-29 0.8097798712038653 1102 30-31 0.7998408619175663 1102 32-33 0.7055600624233662 1102 34-35 0.7671539956338762 1102 36-37 0.7168151524108666 1102 38-39 0.7992840599483415 1102 40-41 0.8203430355223134 1102 42-43 0.660436615508786 1102 44-45 0.7046111566411177 1102 46-47 0.5415510791133045 1102 48-49 0.7253885536475444 1102 50-51 0.7940002176110887 1102 52-53 0.7571857980194707 1102 54-55 0.8038264058693194 1102 56-57 0.7128321731015106 1102 58-59 0.7535682660583838 1102 60-61 0.6803172017558055 1102 62-63 0.7808705676173595 1102 64-65 0.7682952057121355 1102 66-67 0.7684972281894744 1102 68-69 0.704016724084358 1102 70-71 0.7345900427176879 1102 72-73 0.9316742614604188 1102 74-75 0.8577350469045797 1102 76-77 1.0453860076339403 1103 1 0.9134551865050895 1103 2 0.6483578814475237 1103 3 0.6809190280641104 1103 4 0.6634906196146773 1103 5 0.8333696902052594 1103 6 0.786285933710559 1103 7 0.8348252788510777 1103 8 0.7861837413240593 1103 9 0.8038982167753019 1103 10-11 0.8893496336859812 1103 12-13 0.9404508079305387 1103 14-15 0.8808128364465944 1103 16-17 0.8964980090085604 1103 18-19 0.9266477793246821 1103 20-21 0.9966265229671336 1103 22-23 1.1031021005894885 1103 24-25 0.9674734605221289 1103 26-27 0.8803941138583014 1103 28-29 0.839666025354795 1103 30-31 1.063537661724645 1103 32-33 1.1122456316473617 1103 34-35 1.1107740675317217 1103 36-37 1.1494882670872713 1103 38-39 1.1424985645402117 1103 40-41 1.1135862229540834 1103 42-43 1.0321666580097855 1103 44-45 1.0228524278001778 1103 46-47 0.685238631810801 1103 48-49 0.7284017393537958 1103 50-51 0.9734359689247398 1103 52-53 0.693265678066048 1103 54-55 0.7028084713067955 1103 56-57 0.724552849491527 1103 58-59 0.6827793147093111 1103 60-61 0.6349417890571658 1103 62-63 0.698193637895745 1103 64-65 0.5896265759888735 1103 66-67 0.5844351220535167 1103 68-69 0.4265572361894243 1103 70-71 0.46740055152222126 1103 72-73 0.41015766898879846 1103 74-75 0.5056050616642551 1103 76-77 0.6807896621672427 1104 1 0.6189772265762059 1104 2 0.5206806425493511 1104 3 0.46947530686946237 1104 4 0.3181446519983524 1104 5 0.4015826853217064 1104 6 0.10900341107099365 1104 7 0.3884613955506282 1104 8 0.4058918458987293 1104 9 0.49921911736160496 1104 10-11 0.4599569476693546 1104 12-13 0.5816157619390978 1104 14-15 0.5534998789221675 1104 16-17 0.5735038741141949 1104 18-19 0.6004388106105836 1104 20-21 0.5577501155241151 1104 22-23 0.5883462084741709 1104 24-25 0.5377143971122607 1104 26-27 0.3971975443776401 1104 28-29 0.5249077986486483 1104 30-31 0.7523536594998035 1104 32-33 0.617392893206393 1104 34-35 0.6485953762454102 1104 36-37 0.5239960007176165 1104 38-39 0.5067443229976547 1104 40-41 0.4606036383200518 1104 42-43 0.5382894206793694 1104 44-45 0.4958450460625379 1104 46-47 0.37567095031852205 1104 48-49 0.5021762240141676 1104 50-51 0.3152759604997648 1104 52-53 0.48048399775366946 1104 54-55 0.5669567563368183 1104 56-57 0.6552781267710479 1104 58-59 0.528683283615969 1104 60-61 0.35353430003059216 1104 62-63 0.344544024875475 1104 64-65 0.2805500958156877 1104 66-67 0.21173933764292485 1104 68-69 0.20715417913645595 1104 70-71 0.16496632308063042 1104 72-73 0.19903712673662355 1104 74-75 0.25555342801301606 1104 76-77 0.29367897222919126 1105 1 0.07806645367016074 1105 2 0.2603331456885698 1105 3 0.19392524748631246 1105 4 0.22406453304159157 1105 5 0.37717641478758424 1105 6 -0.08751808318046983 1105 7 0.07364227267988355 1105 8 0.2505987869930948 1105 9 -0.07854491998515556 1105 10-11 0.30276913774441283 1105 12-13 0.2406340421540989 1105 14-15 0.023500674778816233 1105 16-17 0.0890661026216506 1105 18-19 0.27043974251110114 1105 20-21 0.2681866871097256 1105 22-23 0.33211999064344155 1105 24-25 0.3264545900496785 1105 26-27 0.09541890680259968 1105 28-29 0.36239642197989497 1105 30-31 0.2691583086630587 1105 32-33 0.47130711346414245 1105 34-35 0.5104897599462674 1105 36-37 0.5993391373723256 1105 38-39 0.5299986420504652 1105 40-41 0.7043105889374885 1105 42-43 0.5249732764891917 1105 44-45 0.28865754223630447 1105 46-47 0.14040059033283114 1105 48-49 0.24352519423008445 1105 50-51 0.3732852373313591 1105 52-53 0.3593263868473038 1105 54-55 0.28374742699229216 1105 56-57 0.5318876958248779 1105 58-59 0.5130375581824325 1105 60-61 0.2994838998575453 1105 62-63 0.18173709289605355 1105 64-65 0.2729486444996674 1105 66-67 0.3336773302067684 1105 68-69 0.1271333440175475 1105 70-71 -0.24204513997597488 1105 72-73 -0.18247138212731429 1105 74-75 -0.31812079044895114 1105 76-77 -0.2740982545709052 1106 1 0.11537308988798145 1106 2 0.2668646955893337 1106 3 -0.04611023085966082 1106 4 0.1183628501989702 1106 5 -0.10311883029846314 1106 6 0.022244578918865443 1106 7 0.11842179039187073 1106 8 0.28311652616608285 1106 9 0.062099535333878464 1106 10-11 -0.09818816590129131 1106 12-13 -0.03537831409742864 1106 14-15 0.003999818404949451 1106 16-17 0.16081358107636134 1106 18-19 0.18623508274979628 1106 20-21 -0.018528983172743096 1106 22-23 0.18153718064969127 1106 24-25 0.11292116428234067 1106 26-27 0.1325345580545374 1106 28-29 0.31218640648361173 1106 30-31 0.22870823661891393 1106 32-33 0.22842249285136518 1106 34-35 0.3209191702716865 1106 36-37 0.2733267811207938 1106 38-39 0.20942120138493436 1106 40-41 0.21713512744712204 1106 42-43 0.373349904279209 1106 44-45 0.10441176293608478 1106 46-47 0.2574166031648488 1106 48-49 0.20776784708602136 1106 50-51 0.061132884342207205 1106 52-53 0.1357876597179235 1106 54-55 0.3256295464496475 1106 56-57 0.16853845840541837 1106 58-59 -0.05474239451310581 1106 60-61 -0.0360505182160189 1106 62-63 -0.23859940766127252 1106 64-65 -0.08461904981564317 1106 66-67 -0.13809087793286068 1106 68-69 -0.09933982293457788 1106 70-71 -0.37555348622288065 1106 72-73 -0.15729331073564268 1106 74-75 -0.28249189938514263 1106 76-77 -0.21164956903682608 1107 1 0.08102678084917159 1107 2 0.09114687332466076 1107 3 0.17311372838739914 1107 4 -0.006814859137428186 1107 5 -0.05305352942713171 1107 6 -0.20367629331884274 1107 7 -0.22057826158892624 1107 8 -0.002669650841511384 1107 9 -0.2129491966596504 1107 10-11 0.05006860947020897 1107 12-13 0.2184426802951407 1107 14-15 0.2515156238148535 1107 16-17 0.2270974579338727 1107 18-19 0.15219204915071316 1107 20-21 0.09356361736136165 1107 22-23 0.0314313510037465 1107 24-25 -0.16811526775602204 1107 26-27 -0.11907343529193071 1107 28-29 0.09359255191287019 1107 30-31 0.21280536273081196 1107 32-33 0.22639784096023874 1107 34-35 0.29018373951335263 1107 36-37 0.24906612668261374 1107 38-39 0.140748400984684 1107 40-41 0.12967435670191207 1107 42-43 0.35993910332015844 1107 44-45 0.22270534720669488 1107 46-47 0.19702418066496108 1107 48-49 0.314443886539447 1107 50-51 0.21256609227187795 1107 52-53 0.174792260018112 1107 54-55 0.16638931071675245 1107 56-57 0.1643333746838529 1107 58-59 0.13510705611488305 1107 60-61 0.016556237337805868 1107 62-63 0.1369641778438826 1107 64-65 0.00932916756803337 1107 66-67 0.2664783962203394 1107 68-69 0.12751564771901513 1107 70-71 0.16480610715736432 1107 72-73 0.2667278259543515 1107 74-75 0.2887280410968458 1107 76-77 0.44912101373283875 1108 1 0.12317463489983282 1108 2 0.05573857061164489 1108 3 0.009162823215753235 1108 4 0.13294676779833736 1108 5 0.0650337842389277 1108 6 0.11237282000190163 1108 7 -0.055968873249362616 1108 8 0.1635476507613447 1108 9 0.16861675737197146 1108 10-11 -0.1780924949939191 1108 12-13 -0.0632876402581033 1108 14-15 0.1340714600437849 1108 16-17 0.07443345971899618 1108 18-19 0.12336826991038663 1108 20-21 -0.011887153124050087 1108 22-23 0.0849870060072675 1108 24-25 0.13736536793835796 1108 26-27 0.0011613841514659384 1108 28-29 0.06274092806533105 1108 30-31 0.05734832024331382 1108 32-33 0.18353556426354345 1108 34-35 0.09552144444754873 1108 36-37 0.16361341141424646 1108 38-39 -0.018677108280307664 1108 40-41 0.11272954908749 1108 42-43 0.17502653533750845 1108 44-45 0.10606544827377107 1108 46-47 0.02721812268145385 1108 48-49 0.1359703685657685 1108 50-51 0.17888012448192114 1108 52-53 0.10219576210682746 1108 54-55 0.20685867221769172 1108 56-57 0.1176805434065642 1108 58-59 0.15234974530281775 1108 60-61 -0.035006169237103535 1108 62-63 0.04641480593789282 1108 64-65 0.05587982839357153 1108 66-67 0.03765549536851864 1108 68-69 0.1474187753368419 1108 70-71 0.03562012364887934 1108 72-73 0.10556904043586712 1108 74-75 0.1254406274485973 1108 76-77 0.07960046283222866 1109 1 0.11835193811012346 1109 2 0.21035457597913165 1109 3 0.04418488369888962 1109 4 0.24736832554362564 1109 5 0.25122070625272386 1109 6 0.25090214173867054 1109 7 0.127220636658528 1109 8 0.2657274181909841 1109 9 0.31810041529998045 1109 10-11 0.053335886505578856 1109 12-13 0.23174558187101724 1109 14-15 0.2678992089469432 1109 16-17 0.383394081679306 1109 18-19 0.22596636717439367 1109 20-21 0.22567912881109464 1109 22-23 0.2827239127163139 1109 24-25 0.3811234261211496 1109 26-27 0.1691892202453218 1109 28-29 0.3531194964682882 1109 30-31 0.22829758576990145 1109 32-33 0.2575004839213406 1109 34-35 0.402808978960536 1109 36-37 0.38756797331190995 1109 38-39 0.3856572278491015 1109 40-41 0.46808597971059385 1109 42-43 0.46784482003059225 1109 44-45 0.4478360532597172 1109 46-47 0.2064608622007924 1109 48-49 0.38419494026465273 1109 50-51 0.41797950950923735 1109 52-53 0.47212455637079387 1109 54-55 0.5916970757003739 1109 56-57 0.7134990228873797 1109 58-59 0.6512446341346774 1109 60-61 0.7750659795188817 1109 62-63 0.6253614990297294 1109 64-65 0.622403161180678 1109 66-67 0.6397510866729448 1109 68-69 0.500773839353986 1109 70-71 0.7482940026145819 1109 72-73 0.6457841262816011 1109 74-75 0.6080472428108585 1109 76-77 0.8357015700304089 1110 1 -0.39119170500854494 1110 2 -0.4669767602868333 1110 3 -0.5512909790731939 1110 4 -0.3666339354005146 1110 5 -0.2675242898341068 1110 6 -0.5995169125629687 1110 7 -0.8030695678159816 1110 8 -0.6484748493500732 1110 9 -0.3971536110697045 1110 10-11 -0.4152414878605981 1110 12-13 -0.318954891008147 1110 14-15 -0.5375543442437163 1110 16-17 -0.6209462413898237 1110 18-19 -0.48979847064634185 1110 20-21 -0.5346130397461906 1110 22-23 -0.671156425165897 1110 24-25 -0.6596902888739464 1110 26-27 -0.4543154530722333 1110 28-29 -0.24783016004212044 1110 30-31 -0.3050924210968873 1110 32-33 -0.2913145444604126 1110 34-35 -0.14880426765329702 1110 36-37 -0.10504617588041043 1110 38-39 -0.1716571063568253 1110 40-41 -0.24330240191518726 1110 42-43 -0.2767214925014514 1110 44-45 -0.2595854059188447 1110 46-47 -0.20628416087343027 1110 48-49 -0.1025142165591113 1110 50-51 -0.23173547359365543 1110 52-53 -0.07657480102929881 1110 54-55 -0.12656057297769152 1110 56-57 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30-31 -0.9336212945439399 2314 32-33 -1.1501446393351173 2314 34-35 -0.916075711562101 2314 36-37 -1.1425186185445568 2314 38-39 -1.180265912428645 2314 40-41 -0.995762815443765 2314 42-43 -0.8325169851224743 2314 44-45 -0.9478570993655602 2314 46-47 -0.9141455858354703 2314 48-49 -0.8498261500049331 2314 50-51 -0.8668620084278373 2314 52-53 -0.7615233891780626 2314 54-55 -0.6998043493716075 2314 56-57 -0.6716202813008358 2314 58-59 -0.838202406105534 2314 60-61 -0.9129259488309636 2314 62-63 -0.8314376659536862 2314 64-65 -0.8934193155665788 2314 66-67 -0.9368608229235136 2314 68-69 -0.802919941921008 2314 70-71 -0.8052250432171633 2314 72-73 -0.6603540944275004 2314 74-75 -0.5700195134229205 2314 76-77 -0.5865609853909 2315 1 -1.3220218328924815 2315 2 -1.219612808809245 2315 3 -1.2690715233443726 2315 4 -1.0826041394957784 2315 5 -1.0639086200043835 2315 6 -1.1885605709121734 2315 7 -1.2831959100577635 2315 8 -1.3183287219697561 2315 9 -1.1698055390929127 2315 10-11 -1.255105458609421 2315 12-13 -1.3429798305606866 2315 14-15 -1.474478551746131 2315 16-17 -1.5013282487334436 2315 18-19 -1.298978004076801 2315 20-21 -1.3814555757384426 2315 22-23 -1.35308000314118 2315 24-25 -1.4540671340950695 2315 26-27 -1.4994822263730931 2315 28-29 -1.3422093820493615 2315 30-31 -1.4225750320157076 2315 32-33 -1.459289407056481 2315 34-35 -1.3387779559885402 2315 36-37 -1.6782270351721067 2315 38-39 -1.534619070070363 2315 40-41 -1.4082027953054137 2315 42-43 -1.523780894168027 2315 44-45 -1.427717778773637 2315 46-47 -1.2839852177081674 2315 48-49 -1.2748511240648703 2315 50-51 -1.170111704704631 2315 52-53 -1.3866122900125752 2315 54-55 -1.501813411631172 2315 56-57 -1.5280201753812719 2315 58-59 -1.3730460781503844 2315 60-61 -1.4390069932069416 2315 62-63 -1.541806531966575 2315 64-65 -1.5356031451042753 2315 66-67 -1.4419413820242397 2315 68-69 -1.348577751272611 2315 70-71 -1.5337666149407276 2315 72-73 -2.8303146473458014 2315 74-75 -3.4319776842875385 2315 76-77 -3.0096115597090396 2316 1 -3.4592905531555758 2316 2 -3.5146046332170116 2316 3 -3.0487447102981804 2316 4 -2.9639533886841463 2316 5 -3.551422476382534 2316 6 -3.1185337928854224 2316 7 -3.5760856462007276 2316 8 -3.484325894083746 2316 9 -3.242235098664146 2316 10-11 -3.1698945915226986 2316 12-13 -3.1579189809836876 2316 14-15 -3.319186007728277 2316 16-17 -4.589488253367598 2316 18-19 -4.3318572475514685 2316 20-21 -3.7415447305059573 2316 22-23 -3.9062643370739742 2316 24-25 -3.939550520923021 2316 26-27 -3.680208366414007 2316 28-29 -3.8699974433149578 2316 30-31 -3.9870638007794206 2316 32-33 -4.118149326643405 2316 34-35 -4.003161073983009 2316 36-37 -3.901223159335789 2316 38-39 -3.673754616386873 2316 40-41 -3.807262470546135 2316 42-43 -4.0675573726863625 2316 44-45 -3.8473894253984717 2316 46-47 -3.716502747178481 2316 48-49 -3.8435474923114477 2316 50-51 -4.078921464860926 2316 52-53 -5.084403892718289 2316 54-55 -4.317270217910448 2316 56-57 -4.2729905405050665 2316 58-59 -4.478619304336505 2316 60-61 -4.34720825759322 2316 62-63 -4.1657914288435265 2316 64-65 -4.1471754260286815 2316 66-67 -4.2845626138134705 2316 68-69 -4.268851376891046 2316 70-71 -4.06518248006584 2316 72-73 -4.1892820608306565 2316 74-75 -4.191237496973002 2316 76-77 -4.120974448049676 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76600.0 3 6492.0 4 8270.0 5 9554.0 6 10936.0 7 11568.0 8 13342.0 9 13999.0 10 16822.0 11 18446.0 12 15166.0 13 13277.0 14 13196.0 15 13824.0 16 13696.0 17 14477.0 18 15273.0 19 16082.0 20 17283.0 21 18332.0 22 20763.0 23 23464.0 24 27416.0 25 31769.0 26 37491.0 27 44433.0 28 51885.0 29 60692.0 30 71410.0 31 85890.0 32 104391.0 33 131503.0 34 174016.0 35 232250.0 36 323752.0 37 408597.0 38 321513.0 39 73500.0 40 536.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.593652895452657 25.777929414850924 27.681061431752386 22.94735625794403 2 23.285197921761966 26.085837992218124 27.519008679364752 23.109955406655157 3 23.4754351535603 26.00626881904709 27.21529438816162 23.303001639230985 4 23.210263949882666 26.06502651366831 27.557896536767974 23.166812999681046 5 23.573600771395892 25.948649081234702 27.294102491030962 23.183647656338447 6 23.461845204100314 25.92150315206134 27.41401366381214 23.202637980026207 7 23.38031490130201 25.67033938304143 27.387652786393602 23.56169292926296 8 23.225240742924115 26.126883018420067 27.227081890463435 23.420794348192388 9 23.20322473598938 25.947334517558414 27.539909035897637 23.30953171055457 10-11 23.71936884890529 25.91714594866407 27.122822490267467 23.240662712163175 12-13 23.713936362546796 25.758965816233513 27.021869006148403 23.505228815071295 14-15 23.459091839760035 25.879539547147584 27.242796811720986 23.418571801371396 16-17 23.63523691858479 25.76497660774199 27.300570509555012 23.299215964118208 18-19 23.538958187014757 25.81959549119512 27.08093806815365 23.560508253636474 20-21 23.462187085239155 25.954749085488633 27.07398723441885 23.509076594853358 22-23 23.64567531813212 26.08973269074116 26.735181374691503 23.529410616435214 24-25 23.71091579132914 26.294013075845406 26.352456752362585 23.642614380462867 26-27 23.606944924344912 26.27416990233456 26.487636137188414 23.63124903613211 28-29 23.880980682636626 26.14240236406896 26.375315466187292 23.60130148710712 30-31 23.55341598867775 26.094696003386836 26.53356789280791 23.818320115127506 32-33 23.505567754843117 26.430547016054263 26.48763968184258 23.57624554726004 34-35 23.736646308983296 26.04650717938698 26.43212486548958 23.784721646140145 36-37 23.57707581245053 26.04089017973995 26.787215499581805 23.59481850822771 38-39 23.557190336979126 26.13534795592571 26.785855453665835 23.521606253429336 40-41 23.407730122062265 26.216225975731987 26.64230691530009 23.733736986905658 42-43 23.340793729987517 26.38669293225991 26.48116786634217 23.791345471410402 44-45 23.318518171508433 26.0525108673799 26.847454583428494 23.78151637768317 46-47 23.354568610364097 26.103890830608158 26.658315451816733 23.883225107211015 48-49 23.43924714054584 26.16648472979642 26.51954376792039 23.87472436173735 50-51 23.289241686276586 26.370841137114276 26.55971329854082 23.780203878068317 52-53 23.544343439688028 26.199074888500263 26.505629606691915 23.75095206511979 54-55 23.393074954044906 26.289495220201815 26.537972884543002 23.779456941210277 56-57 23.35346828121047 26.46608239920293 26.51568448932296 23.66476483026364 58-59 23.368354027379816 26.47024548840897 26.425583904339884 23.735816579871326 60-61 23.303295541922736 26.305460972376466 26.417431666350755 23.973811819350043 62-63 23.441014542605064 26.253761371964206 26.441190629669382 23.864033455761348 64-65 23.316096465293533 26.311735158310896 26.359309148359205 24.012859228036366 66-67 23.269277397031622 26.338796790097135 26.510532762194583 23.881393050676657 68-69 23.400656932753133 26.08938354419184 26.60120768942853 23.9087518336265 70-71 23.253772688316126 25.961266396190023 26.700738886676902 24.084222028816953 72-73 23.436728135524092 25.891751601669142 26.5226136208925 24.148906641914262 74-75 23.278051993486017 25.9102102589556 26.427609690027186 24.38412805753119 76-77 23.266600875753625 25.76450681818892 26.762876109928648 24.206016196128807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6162.0 1 3736.0 2 1260.0 3 1261.0 4 1312.0 5 1409.5 6 1533.5 7 1611.5 8 1748.0 9 1833.0 10 1909.5 11 2052.5 12 2182.5 13 2246.0 14 2399.0 15 2753.0 16 3140.0 17 3326.0 18 3603.5 19 4273.5 20 5207.5 21 6504.5 22 7260.0 23 8513.0 24 11127.0 25 14367.0 26 16246.0 27 17616.5 28 22553.0 29 27794.0 30 29469.0 31 33138.5 32 39924.0 33 45987.5 34 53282.0 35 57629.0 36 61868.5 37 70212.5 38 76634.0 39 78951.0 40 83340.0 41 92213.0 42 102610.0 43 108523.0 44 110243.5 45 113738.5 46 119103.0 47 124697.0 48 126701.0 49 128079.0 50 130323.0 51 129058.0 52 126927.0 53 126699.0 54 122844.0 55 112631.0 56 100545.0 57 95045.0 58 91108.5 59 78774.5 60 64837.0 61 59297.0 62 52204.5 63 38975.0 64 28574.0 65 24310.0 66 21293.0 67 16280.0 68 12202.0 69 8588.5 70 7057.0 71 5872.0 72 3943.0 73 2931.0 74 2663.0 75 2286.5 76 1567.5 77 1021.5 78 818.0 79 701.5 80 491.0 81 317.5 82 204.0 83 170.0 84 139.5 85 94.5 86 79.0 87 78.0 88 76.0 89 62.5 90 49.0 91 47.0 92 55.5 93 57.5 94 43.0 95 33.5 96 32.0 97 36.0 98 47.5 99 90.0 100 125.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00913382458216656 2 0.012217466214607406 3 0.013232335612625913 4 0.015496275038974888 5 0.012334566529763388 6 0.009719326157946465 7 0.015808542546057507 8 0.0179553816572505 9 0.01779924790370919 10-11 0.02039497155633345 12-13 0.016667278190534703 14-15 0.00821653878011137 16-17 0.006284383580037675 18-19 0.016745345067305357 20-21 0.022073409406902517 22-23 0.019887536857324195 24-25 0.018170065568369802 26-27 0.023849430853434904 28-29 0.008626389883157306 30-31 0.00876300691750595 32-33 0.010968396186276937 34-35 0.011768581673176143 36-37 0.01126114697416689 38-39 0.014344788606607735 40-41 0.011846648549946798 42-43 0.014832706586424325 44-45 0.013310402489396567 46-47 0.023498129907966958 48-49 0.022561327386719107 50-51 0.0356960794033817 52-53 0.04002879106415302 54-55 0.027713741253582294 56-57 0.020375454837140785 58-59 0.016413560841030076 60-61 0.02035593811794812 62-63 0.02626950403332519 64-65 0.024688649778719436 66-67 0.027596640938426314 68-69 0.04159012859956611 70-71 0.05326112667677893 72-73 0.0840975430011874 74-75 0.08515144583759124 76-77 0.08907430639531662 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2561906.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.286830143819444 #Duplication Level Percentage of deduplicated Percentage of total 1 74.79590926284764 39.108410030790466 2 12.731842011168121 13.31415321311784 3 4.90256688940625 7.690190466452935 4 2.433939087505497 5.090518385952112 5 1.3822266709123125 3.6136125581124556 6 0.8129138808836645 2.550281400679034 7 0.574210789912275 2.101656342722406 8 0.41426684900041244 1.732856029431989 9 0.28780113905516197 1.3543388345677512 >10 1.5132584013604469 14.045961821168223 >50 0.10243721722417688 3.669484009589466 >100 0.04540230967331344 4.2108766885870255 >500 0.0024003654331081507 0.7859907167700597 >1k 7.501141978401236E-4 0.49234238860017354 >5k 7.501141978401236E-5 0.23932711345799876 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6102 0.23818204102726642 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.684012606239261E-4 0.0 3 0.0 0.0 0.0 8.197022060918706E-4 0.0 4 0.0 0.0 0.0 0.0016784378505690686 0.0 5 0.0 0.0 0.0 0.0033959091395234643 0.0 6 0.0 0.0 0.0 0.0060501829497257124 0.0 7 0.0 0.0 0.0 0.011514864323671516 0.0 8 0.0 0.0 0.0 0.01834571604110377 0.0 9 0.0 0.0 0.0 0.025371734950462663 0.0 10 0.0 0.0 0.0 0.03395909139523464 0.0 11 0.0 0.0 0.0 0.044146818813805035 0.0 12 0.0 0.0 0.0 0.057808522248669544 0.0 13 0.0 0.0 0.0 0.0758419707826907 0.0 14 0.0 0.0 0.0 0.09801296378555653 3.9033438385327175E-5 15 0.0 0.0 0.0 0.12248692965315668 7.806687677065435E-5 16 0.0 0.0 0.0 0.1488345005632525 1.1710031515598153E-4 17 0.0 0.0 0.0 0.173230399554082 1.1710031515598153E-4 18 0.0 0.0 0.0 0.20000733828641645 1.1710031515598153E-4 19 0.0 0.0 0.0 0.22834561455416397 1.1710031515598153E-4 20 0.0 0.0 0.0 0.26004076652304964 1.1710031515598153E-4 21 0.0 0.0 0.0 0.29595152983755063 1.1710031515598153E-4 22 0.0 0.0 0.0 0.33400913226324463 1.1710031515598153E-4 23 0.0 0.0 0.0 0.37265223626471855 1.1710031515598153E-4 24 0.0 0.0 0.0 0.41160760777327504 1.9516719192663588E-4 25 0.0 0.0 0.0 0.45169494899500606 1.9516719192663588E-4 26 0.0 0.0 0.0 0.49549046686334314 1.9516719192663588E-4 27 0.0 0.0 0.0 0.5392469512932949 1.9516719192663588E-4 28 0.0 0.0 0.0 0.5847599404505864 1.9516719192663588E-4 29 0.0 0.0 0.0 0.629453227401786 2.3420063031196305E-4 30 0.0 0.0 0.0 0.6779327578763623 2.3420063031196305E-4 31 0.0 0.0 0.0 0.7287933280924437 2.3420063031196305E-4 32 0.0 0.0 0.0 0.7850795462440855 2.3420063031196305E-4 33 0.0 0.0 0.0 0.8462839776322785 2.3420063031196305E-4 34 0.0 0.0 0.0 0.9129530903944173 2.3420063031196305E-4 35 0.0 0.0 0.0 0.9813396744455105 2.3420063031196305E-4 36 0.0 0.0 0.0 1.0540980035957603 2.732340686972902E-4 37 0.0 0.0 0.0 1.1277150683904873 2.732340686972902E-4 38 0.0 0.0 0.0 1.1990291603204801 2.732340686972902E-4 39 0.0 0.0 0.0 1.273817228266767 2.732340686972902E-4 40 0.0 0.0 0.0 1.3489175637201365 2.732340686972902E-4 41 0.0 0.0 0.0 1.4343617603456176 2.732340686972902E-4 42 0.0 0.0 0.0 1.527924912155247 2.732340686972902E-4 43 0.0 0.0 0.0 1.6251962406114822 2.732340686972902E-4 44 0.0 0.0 0.0 1.7307426580054068 2.732340686972902E-4 45 0.0 0.0 0.0 1.838240747318598 2.732340686972902E-4 46 0.0 0.0 0.0 1.9574098347090019 3.122675070826174E-4 47 0.0 0.0 0.0 2.0764618217842497 3.122675070826174E-4 48 0.0 0.0 0.0 2.196762878887828 3.122675070826174E-4 49 0.0 0.0 0.0 2.316400367538856 3.122675070826174E-4 50 0.0 0.0 0.0 2.439785066274875 3.122675070826174E-4 51 0.0 0.0 0.0 2.566916975095886 3.122675070826174E-4 52 0.0 0.0 0.0 2.7067347513921276 3.122675070826174E-4 53 0.0 0.0 0.0 2.8531101453371046 3.122675070826174E-4 54 0.0 0.0 0.0 3.00709705976722 3.122675070826174E-4 55 3.9033438385327175E-5 0.0 0.0 3.1650653849126393 3.122675070826174E-4 56 3.9033438385327175E-5 0.0 0.0 3.3404816570162996 3.122675070826174E-4 57 3.9033438385327175E-5 0.0 0.0 3.513516889378455 3.513009454679446E-4 58 3.9033438385327175E-5 0.0 0.0 3.695568846007621 3.9033438385327175E-4 59 3.9033438385327175E-5 0.0 0.0 3.87266355596185 3.9033438385327175E-4 60 3.9033438385327175E-5 0.0 0.0 4.057174619209292 3.9033438385327175E-4 61 3.9033438385327175E-5 0.0 0.0 4.249999804832808 4.2936782223859893E-4 62 3.9033438385327175E-5 0.0 0.0 4.446767367733242 4.2936782223859893E-4 63 3.9033438385327175E-5 0.0 0.0 4.656376931862449 4.2936782223859893E-4 64 3.9033438385327175E-5 0.0 0.0 4.868445602609932 4.2936782223859893E-4 65 3.9033438385327175E-5 0.0 0.0 5.092068171119471 4.2936782223859893E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCGT 455 0.0 26.522657 1 CGCGGAA 390 0.0 26.396252 59 GCGTTCG 125 9.611722E-8 25.556858 10 CTCGCGC 460 0.0 25.462273 36 TCGCGCT 490 0.0 25.354523 37 ATTCGCG 410 0.0 25.10961 56 AATTCGC 440 0.0 24.20346 55 CCTCGCG 530 0.0 24.10836 35 CGCGCTT 525 0.0 22.9881 38 CGTTCGG 140 2.873112E-7 22.818624 11 CAATTCG 470 0.0 22.661657 54 CGCTCCG 560 0.0 22.18564 25 CGGTTAC 280 0.0 21.548399 4 TCGCGGA 485 0.0 20.493526 58 GATCGTT 605 0.0 19.946793 2 ATCGTTC 625 0.0 19.875229 3 GCGCCCT 635 0.0 18.44547 31 GACGCTC 715 0.0 17.873674 23 ACGCTCC 745 0.0 17.629736 24 AAACTCG 700 0.0 17.242085 12 >>END_MODULE