FastQCFastQC Report
Thu 26 May 2016
SRR936019_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936019_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2561906
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69210.2701504270648494No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50850.19848503418938868No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34110.133143058332351No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA10150.049.559464100-101
CGTACAT13150.042.327423100-101
GGTATCA69700.039.0974271
GCGTATC3300.036.078793100-101
CGGTATC10050.035.244133100-101
GTATCAA134600.030.501381
ACCGTAT2850.029.24081298-99
AGTATCA27900.029.018213100-101
CCGTATC5950.029.014626100-101
CCGTACA6950.027.837772100-101
TCGTACA8300.027.613317100-101
ACGTATC4350.026.001612100-101
GGGTATC12750.025.913061100-101
CGAGTAC12250.025.51286100-101
TACGCAG8200.025.40915100-101
TCGTATC4450.024.74843100-101
CGAATCG2900.024.62733396-97
GCGTACA6450.024.458065100-101
AACGTAC1950.024.42089798-99
ACGTACA7750.024.196068100-101
Produced by FastQC (version 0.11.5)