FastQCFastQC Report
Thu 26 May 2016
SRR936018_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936018_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573428
Sequences flagged as poor quality0
Sequence length77
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62990.24477078822488915No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG4400.032.26928356
TCGCGCT4900.028.97593537
CGATCGT5150.028.2574711
CTCGCGC5000.027.68650636
CGCGGAA4950.027.25279459
TCGCGGA5350.026.54180158
AATTCGC5350.026.54025555
CAATTCG5600.025.35492954
CGCGCTT5350.024.54925738
CGGTTAC4050.024.5410674
AACTCGA5850.024.27138313
GATCGTT6350.022.3589482
CCTCGCG6050.022.29340735
ACTCGAG6400.022.18426914
ACGCGAA1153.6974638E-521.60680854
CGCTCCG6600.021.511625
CGGCCAA6850.021.25127650
ATCGTTC6700.021.1909453
AAACTCG7100.021.00102212
ACGCTCC7300.019.93545324
Produced by FastQC (version 0.11.5)