##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936018_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2573428 Sequences flagged as poor quality 0 Sequence length 77 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.22766481129451 39.0 36.0 41.0 29.0 41.0 2 36.0926965899182 39.0 36.0 41.0 28.0 41.0 3 35.95464920720533 39.0 35.0 41.0 27.0 41.0 4 35.839796955656034 39.0 35.0 41.0 27.0 41.0 5 35.75890213365208 39.0 35.0 40.0 27.0 41.0 6 35.66361988755854 39.0 35.0 40.0 27.0 41.0 7 35.51269512883205 39.0 35.0 40.0 27.0 41.0 8 35.3797805883825 38.0 35.0 40.0 27.0 41.0 9 35.534269076111705 39.0 35.0 40.0 27.0 41.0 10-11 35.753742284610254 39.0 35.0 41.0 27.5 41.0 12-13 35.66528129017016 39.0 35.0 41.0 27.0 41.0 14-15 35.49652020573336 39.0 35.0 41.0 26.5 41.0 16-17 35.325577789625356 39.0 35.0 41.0 26.0 41.0 18-19 35.146432307412525 39.0 35.0 41.0 25.0 41.0 20-21 34.92524543138568 39.0 34.0 41.0 24.5 41.0 22-23 34.619065114703034 38.5 34.0 40.5 23.5 41.0 24-25 34.36491772841517 38.0 34.0 40.0 22.5 41.0 26-27 33.39060486634948 37.5 32.0 39.5 20.5 40.5 28-29 33.37430151533286 37.5 32.5 39.5 20.0 40.5 30-31 33.747483706557944 38.0 33.0 40.0 20.0 41.0 32-33 33.59087878114329 38.0 33.0 40.0 18.5 41.0 34-35 33.30778653997703 37.0 33.0 40.0 18.0 41.0 36-37 32.96832784907913 37.0 32.0 40.0 14.5 41.0 38-39 32.6316426960459 36.0 32.0 40.0 12.5 41.0 40-41 32.24442922047946 36.0 31.5 39.0 8.0 41.0 42-43 31.857157845488587 35.0 31.0 39.0 7.5 41.0 44-45 31.48107815722841 35.0 31.0 38.5 4.5 40.0 46-47 31.050928372583186 35.0 31.0 37.5 2.0 40.0 48-49 30.63229241307703 35.0 30.0 37.0 2.0 39.0 50-51 30.18123316447944 35.0 30.0 36.5 2.0 39.0 52-53 29.705078595554255 34.0 29.0 36.0 2.0 38.5 54-55 29.25641556709572 34.0 29.0 35.5 2.0 37.5 56-57 28.835214935098243 34.0 29.0 35.0 2.0 37.0 58-59 28.423339413420543 34.0 28.5 35.0 2.0 36.5 60-61 28.080103076518945 34.0 27.5 35.0 2.0 36.0 62-63 27.71341922136543 34.0 27.0 35.0 2.0 36.0 64-65 27.336016395251782 33.5 26.0 35.0 2.0 35.0 66-67 26.978453448085588 33.0 25.5 35.0 2.0 35.0 68-69 26.594848000410348 33.0 25.0 35.0 2.0 35.0 70-71 26.12819126861136 33.0 24.0 35.0 2.0 35.0 72-73 25.629285140287585 33.0 21.5 35.0 2.0 35.0 74-75 25.032226664200437 32.5 18.0 35.0 2.0 35.0 76-77 23.277336105770203 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.21453541863945 1101 2 1.1030400403230516 1101 3 1.2278360185907644 1101 4 1.0142604092754226 1101 5 1.1718891213413798 1101 6 1.200693845414662 1101 7 1.0843976762976553 1101 8 1.1016684885833286 1101 9 1.0611919917401593 1101 10-11 1.0752354786592733 1101 12-13 1.3568965604184484 1101 14-15 1.3338634993834972 1101 16-17 1.2532314973002343 1101 18-19 1.320153120210172 1101 20-21 1.2061632386681111 1101 22-23 1.235287739418105 1101 24-25 1.2497465270279875 1101 26-27 1.1317422851670997 1101 28-29 1.249187138015138 1101 30-31 1.4014604817602176 1101 32-33 1.4133057119233783 1101 34-35 1.475449396960407 1101 36-37 1.3696646188987103 1101 38-39 1.35628301330145 1101 40-41 1.2295276982979573 1101 42-43 1.355230513462665 1101 44-45 1.2039515503898848 1101 46-47 1.2454461193372026 1101 48-49 1.1678209110350615 1101 50-51 1.0617269771237368 1101 52-53 0.9828058126235604 1101 54-55 1.1409247870054635 1101 56-57 1.1876355711189674 1101 58-59 1.1509816205695138 1101 60-61 1.2217592549617713 1101 62-63 1.1433634513039088 1101 64-65 1.1388725076913566 1101 66-67 1.1730510059818293 1101 68-69 1.1593516031205944 1101 70-71 1.0658037998510927 1101 72-73 1.1120137557138712 1101 74-75 1.208084406547183 1101 76-77 1.3410906787462729 1102 1 1.1056613428148196 1102 2 1.1070582538427658 1102 3 1.1320542840470438 1102 4 0.9204585843510955 1102 5 1.0106656031477854 1102 6 1.1377004996147235 1102 7 0.8549734164279883 1102 8 1.0284214505473557 1102 9 1.0936059700944298 1102 10-11 1.1380735643710835 1102 12-13 1.2277907554773364 1102 14-15 1.3191416141687924 1102 16-17 1.3725432073458421 1102 18-19 1.2132871206580873 1102 20-21 1.24544867815721 1102 22-23 1.1579656393124438 1102 24-25 1.308006672862554 1102 26-27 1.2268313604902303 1102 28-29 1.3193791246710092 1102 30-31 1.5312946694820297 1102 32-33 1.4864493577325248 1102 34-35 1.510198974804105 1102 36-37 1.4646595763808108 1102 38-39 1.4742444414744256 1102 40-41 1.4120440040421727 1102 42-43 1.3231069607820984 1102 44-45 1.1163331645726906 1102 46-47 1.261068650461354 1102 48-49 1.1686987144757914 1102 50-51 1.1827795237326768 1102 52-53 1.3701793890053402 1102 54-55 1.3885632535853851 1102 56-57 1.34021725097816 1102 58-59 1.3983399663488463 1102 60-61 1.206314311150809 1102 62-63 1.1105266296565297 1102 64-65 1.014425020637713 1102 66-67 1.0773163303586237 1102 68-69 1.1886284346056897 1102 70-71 1.2910593950536935 1102 72-73 1.2306276541023244 1102 74-75 0.9300556669430797 1102 76-77 1.0408130311152277 1103 1 0.5474118281395022 1103 2 0.43997172618805536 1103 3 0.49457063601338547 1103 4 0.5538711999454691 1103 5 0.5198610612792152 1103 6 0.5588465861139014 1103 7 0.6139987806099398 1103 8 0.4976643440372186 1103 9 0.4956951693380134 1103 10-11 0.6135456986104657 1103 12-13 0.7540376504880797 1103 14-15 0.7277548501747759 1103 16-17 0.5779520441913206 1103 18-19 0.586231630333593 1103 20-21 0.5972970994073137 1103 22-23 0.43340084030135273 1103 24-25 0.6129079078233488 1103 26-27 0.5270578174273979 1103 28-29 0.48482618449952497 1103 30-31 0.6176868185282203 1103 32-33 0.5636638034071453 1103 34-35 0.5781758366670502 1103 36-37 0.6191743103190319 1103 38-39 0.6399737349857304 1103 40-41 0.6887529415537941 1103 42-43 0.6678967762498402 1103 44-45 0.6322383307053165 1103 46-47 0.6069539848622014 1103 48-49 0.48753442097286026 1103 50-51 0.5664721955392906 1103 52-53 0.6705080195606143 1103 54-55 0.6280805753636081 1103 56-57 0.5534733566893308 1103 58-59 0.5625709088753474 1103 60-61 0.39619317678915067 1103 62-63 0.395360798524397 1103 64-65 0.32390472242474644 1103 66-67 0.27876359453437516 1103 68-69 0.44890349938544105 1103 70-71 0.511109460126562 1103 72-73 0.4870702032168488 1103 74-75 0.5326049824635923 1103 76-77 0.6621044218991656 1104 1 0.4775580690607839 1104 2 0.509886827935091 1104 3 0.33637689132974913 1104 4 0.4677485473249092 1104 5 0.449381606983593 1104 6 0.44062863152383613 1104 7 0.4794563151254181 1104 8 0.4316252810260295 1104 9 0.3896083408357569 1104 10-11 0.47070438800390946 1104 12-13 0.69014558086824 1104 14-15 0.7221276827338272 1104 16-17 0.6582033855510048 1104 18-19 0.48531803352668845 1104 20-21 0.6242490231133857 1104 22-23 0.5946776674949703 1104 24-25 0.6417953971906201 1104 26-27 0.426886466907213 1104 28-29 0.38743488370779744 1104 30-31 0.776680880557926 1104 32-33 0.76690555895253 1104 34-35 0.8826497619435045 1104 36-37 0.9359036828244314 1104 38-39 0.7347641066405117 1104 40-41 0.7789708307116854 1104 42-43 0.9224561723108167 1104 44-45 0.7342810415605179 1104 46-47 0.6464468036804973 1104 48-49 0.5965301188070562 1104 50-51 0.482367307755478 1104 52-53 0.39612635365732274 1104 54-55 0.4955239765283004 1104 56-57 0.4682810678716933 1104 58-59 0.47295172416030695 1104 60-61 0.39859658253410046 1104 62-63 0.30699832060775734 1104 64-65 0.31579235901442004 1104 66-67 0.4207420182731738 1104 68-69 0.06842519015296133 1104 70-71 0.09439358300819833 1104 72-73 0.2033779471152961 1104 74-75 0.14412145501479046 1104 76-77 0.13742340377445217 1105 1 0.3811286651421639 1105 2 0.5423703816316277 1105 3 0.5709701366904696 1105 4 0.5452752240061258 1105 5 0.2596037292042297 1105 6 0.49732661523292876 1105 7 0.47203977687804866 1105 8 0.39534402333706 1105 9 0.5760249244075837 1105 10-11 0.6517523704180164 1105 12-13 0.697814964844099 1105 14-15 0.6344766551636027 1105 16-17 0.6196650507716015 1105 18-19 0.5664026801901585 1105 20-21 0.41102277960449385 1105 22-23 0.4937549100844194 1105 24-25 0.5265480875209718 1105 26-27 0.319107899548591 1105 28-29 0.5655356432162222 1105 30-31 0.6258210917699003 1105 32-33 0.7177659952328099 1105 34-35 0.6226955396464575 1105 36-37 0.5493284636483011 1105 38-39 0.6156751444411128 1105 40-41 0.7097953473587069 1105 42-43 0.6939636799836038 1105 44-45 0.5538685728020205 1105 46-47 0.5849547063235612 1105 48-49 0.35895266254515334 1105 50-51 0.37234445127861093 1105 52-53 0.27916238012680594 1105 54-55 0.07757209167832357 1105 56-57 0.1950309966475423 1105 58-59 0.2825857615335572 1105 60-61 0.3363503185644525 1105 62-63 0.2662128639314645 1105 64-65 0.343412679264091 1105 66-67 0.35659763073610407 1105 68-69 0.2149080121832334 1105 70-71 0.05335916555698361 1105 72-73 -0.43909001820501814 1105 74-75 -0.5085409522319431 1105 76-77 -0.4567750655538383 1106 1 -0.026422493387514123 1106 2 -0.10401670804505159 1106 3 -0.13288489527074887 1106 4 -0.11103557178179102 1106 5 -0.09468115149449119 1106 6 -0.13892575226442005 1106 7 -0.4759484615495211 1106 8 -0.2885825353511393 1106 9 -0.2992822712403438 1106 10-11 -0.5028041934683856 1106 12-13 -0.20203933435094967 1106 14-15 -0.17255542313879602 1106 16-17 -0.32686304331582505 1106 18-19 -0.1430615673831639 1106 20-21 -0.140040937597945 1106 22-23 -0.32147462779038705 1106 24-25 -0.5470448116596671 1106 26-27 -0.614893930115116 1106 28-29 -0.38635285409203846 1106 30-31 -0.23499845153471455 1106 32-33 -0.28273774129561957 1106 34-35 -0.34659832531870194 1106 36-37 -0.33481462776283877 1106 38-39 -0.20905180527015688 1106 40-41 -0.25716182023951717 1106 42-43 -0.1876580750010035 1106 44-45 -0.3078780258505667 1106 46-47 -0.10531343205150989 1106 48-49 -0.11023296018384698 1106 50-51 -0.2902714949740499 1106 52-53 -0.39092260209123 1106 54-55 -0.24767934233025812 1106 56-57 -0.15284563808364382 1106 58-59 -0.2503410281143026 1106 60-61 -0.4053219451747516 1106 62-63 -0.24122593497536826 1106 64-65 -0.18108134859517122 1106 66-67 -0.37170912936950984 1106 68-69 -0.3326449091026227 1106 70-71 -0.5552264542126508 1106 72-73 -0.6620687030087744 1106 74-75 -0.7203895044558486 1106 76-77 -0.7667277542073485 1107 1 -0.1874848173833641 1107 2 -0.2438913745635105 1107 3 -0.0909570013919847 1107 4 -0.3922031277325857 1107 5 -0.21944725124252784 1107 6 -0.4975045650701162 1107 7 -0.49047111976798874 1107 8 -0.23351083681846063 1107 9 -0.3873464108579512 1107 10-11 -0.14488817523805864 1107 12-13 -0.24721612588793107 1107 14-15 -0.45019502221986585 1107 16-17 -0.30739073154763474 1107 18-19 -0.3322537546515747 1107 20-21 -0.20137213109134677 1107 22-23 -0.039543306462398675 1107 24-25 -0.3223951988687972 1107 26-27 -0.4058964126999669 1107 28-29 -0.3362773575750637 1107 30-31 -0.14346448273376922 1107 32-33 0.034316134501040096 1107 34-35 -0.130690097587852 1107 36-37 -0.06076018690022522 1107 38-39 -0.020388093620539394 1107 40-41 0.09668479677544894 1107 42-43 0.04503099050959136 1107 44-45 0.041064791327439565 1107 46-47 -0.02406760057085222 1107 48-49 0.0883106054749625 1107 50-51 -0.10148967527640806 1107 52-53 -0.10182721710530984 1107 54-55 -0.09661428563839536 1107 56-57 -0.12066906886769857 1107 58-59 -0.13423641680557452 1107 60-61 -0.1684861292565678 1107 62-63 -0.22276541548522388 1107 64-65 -0.1219636388723373 1107 66-67 -0.1875474186091708 1107 68-69 -0.32312493726329095 1107 70-71 -0.3429483120754 1107 72-73 -0.2839608402549558 1107 74-75 -0.3974422686473389 1107 76-77 -0.4662747751055285 1108 1 0.21576874877990093 1108 2 0.31351432292359505 1108 3 0.26807077791615086 1108 4 0.21328662241745633 1108 5 0.3039080972127053 1108 6 0.2279144898736689 1108 7 0.304782369999792 1108 8 0.17661347479578637 1108 9 0.1378844467474778 1108 10-11 0.24248357767837803 1108 12-13 0.32829184899264874 1108 14-15 0.2482797125445586 1108 16-17 0.3739918357373213 1108 18-19 0.33844960535406443 1108 20-21 0.30728164089898513 1108 22-23 0.3265946887584832 1108 24-25 0.3287745702625742 1108 26-27 0.30519628514046104 1108 28-29 0.401898053803599 1108 30-31 0.4513691991539659 1108 32-33 0.43568691925161573 1108 34-35 0.3727002458759614 1108 36-37 0.49176898111621625 1108 38-39 0.46047593931179165 1108 40-41 0.42551689345687294 1108 42-43 0.37641256612239005 1108 44-45 0.2965010279095601 1108 46-47 0.30359564519415727 1108 48-49 0.41335407377120603 1108 50-51 0.19800964046980596 1108 52-53 0.3383130273749302 1108 54-55 0.38685608209567945 1108 56-57 0.4219001847410695 1108 58-59 0.4067599713040586 1108 60-61 0.4231025809599309 1108 62-63 0.18414073633464056 1108 64-65 0.23684795590544638 1108 66-67 0.3314596310780118 1108 68-69 0.4822845976277783 1108 70-71 0.4694154890994717 1108 72-73 0.4576516333647298 1108 74-75 0.43589929189204213 1108 76-77 0.582551489074465 1109 1 0.4936592926425476 1109 2 0.3391152916602209 1109 3 0.25481404372129646 1109 4 0.4382976141893309 1109 5 0.4431200200351313 1109 6 0.22650491381279636 1109 7 0.3018283952144003 1109 8 0.36617910626394945 1109 9 0.3165425887655573 1109 10-11 0.2065026515065611 1109 12-13 0.4514683469045764 1109 14-15 0.41770301334808835 1109 16-17 0.3923423980340033 1109 18-19 0.3694748126640022 1109 20-21 0.4949193667050338 1109 22-23 0.5279128979980001 1109 24-25 0.309854042599099 1109 26-27 0.22842135134671793 1109 28-29 0.5019548750562066 1109 30-31 0.6188813193170333 1109 32-33 0.42128156315472154 1109 34-35 0.5199720954004263 1109 36-37 0.5329899515180401 1109 38-39 0.5250086684993676 1109 40-41 0.5921336041636636 1109 42-43 0.5717169249989951 1109 44-45 0.4646025407571521 1109 46-47 0.47455596818426926 1109 48-49 0.6372752368394359 1109 50-51 0.5220502888455805 1109 52-53 0.5160342760402159 1109 54-55 0.6118646637115894 1109 56-57 0.45819501546778696 1109 58-59 0.6487890743024387 1109 60-61 0.4929815473097747 1109 62-63 0.3184732619005537 1109 64-65 0.4135035504617086 1109 66-67 0.49007754540208026 1109 68-69 0.46001461049655035 1109 70-71 0.5407127957136701 1109 72-73 0.4196823285267435 1109 74-75 0.42804108794026163 1109 76-77 0.33074314175492603 1110 1 -0.5715854483547531 1110 2 -0.47416818230934155 1110 3 -0.8915415820108876 1110 4 -0.6653546832463988 1110 5 -0.732938996246979 1110 6 -0.5584815319106724 1110 7 -0.5411629968296054 1110 8 -1.011636023591116 1110 9 -0.8808721947338825 1110 10-11 -0.8008533024711468 1110 12-13 -0.6747392287758203 1110 14-15 -0.6027758289638925 1110 16-17 -0.6914431218106927 1110 18-19 -0.7372929875816183 1110 20-21 -0.8071635775441592 1110 22-23 -0.8501450203594203 1110 24-25 -0.7454067994072702 1110 26-27 -0.6582022189459309 1110 28-29 -0.6258432765819109 1110 30-31 -0.35954898793209367 1110 32-33 -0.381114346226866 1110 34-35 -0.6637944654353518 1110 36-37 -0.4816679091134404 1110 38-39 -0.5897906772733421 1110 40-41 -0.4235462036429176 1110 42-43 -0.6250580626917994 1110 44-45 -0.7098709930323288 1110 46-47 -0.5745587592870507 1110 48-49 -0.5624983869345961 1110 50-51 -0.6178328161307363 1110 52-53 -0.44771864562795116 1110 54-55 -0.4509792159241677 1110 56-57 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-0.8281065135146903 2314 34-35 -0.6855399621452563 2314 36-37 -1.2585397413269845 2314 38-39 -0.7898265908077953 2314 40-41 -0.5807217129868718 2314 42-43 -0.5479191544830329 2314 44-45 -0.5218285181050213 2314 46-47 -0.3435510086912146 2314 48-49 -0.3710018313690391 2314 50-51 -0.27653390867387984 2314 52-53 -0.12930360982819167 2314 54-55 -0.24792367163624718 2314 56-57 -0.236217479111005 2314 58-59 -0.31431357843460006 2314 60-61 -0.18866075277260208 2314 62-63 -0.2695061464751909 2314 64-65 -0.13199665098925095 2314 66-67 -0.05599903675783224 2314 68-69 -0.11111913497721915 2314 70-71 0.054718513328182894 2314 72-73 -0.018770168823600386 2314 74-75 -0.06250098194910336 2314 76-77 -0.08756849389855859 2315 1 -1.476219520073336 2315 2 -1.237630149993599 2315 3 -1.021645179977547 2315 4 -0.9743779382438476 2315 5 -1.1637550653423858 2315 6 -1.151094628238475 2315 7 -1.383383243448442 2315 8 -1.4112864337447704 2315 9 -1.085646328094633 2315 10-11 -1.423427615391681 2315 12-13 -1.3736277637837162 2315 14-15 -1.1286363240573465 2315 16-17 -1.0914039998479836 2315 18-19 -1.2324451243680485 2315 20-21 -1.022090111012247 2315 22-23 -1.1012582408242153 2315 24-25 -1.404575570551323 2315 26-27 -1.4780689660299657 2315 28-29 -1.4703431298745429 2315 30-31 -1.4557363520397004 2315 32-33 -1.517570247499087 2315 34-35 -1.548292513098943 2315 36-37 -1.6992830743911895 2315 38-39 -1.5589858933594876 2315 40-41 -1.549891648065909 2315 42-43 -1.4910731146161709 2315 44-45 -1.4779112334819295 2315 46-47 -1.4970697497999765 2315 48-49 -1.3352504414645914 2315 50-51 -1.1812574800204132 2315 52-53 -1.1639330690931047 2315 54-55 -1.2448198265277277 2315 56-57 -1.5563263175272901 2315 58-59 -1.3745015865357892 2315 60-61 -1.309329987924304 2315 62-63 -1.1830589799015776 2315 64-65 -1.068895671545853 2315 66-67 -1.1980079817268283 2315 68-69 -1.3474491731747165 2315 70-71 -1.2569964849035173 2315 72-73 -1.1936827489797786 2315 74-75 -1.3176675942212022 2315 76-77 -1.3720842362934569 2316 1 -2.741352593556684 2316 2 -2.2534124499051202 2316 3 -2.4152557397709487 2316 4 -3.282776034802829 2316 5 -2.793849041764588 2316 6 -2.546022770948724 2316 7 -2.343896407321317 2316 8 -2.1654197224412925 2316 9 -2.415725956971464 2316 10-11 -2.3638554113695776 2316 12-13 -2.866975285245246 2316 14-15 -4.1969820520757075 2316 16-17 -3.2314886503656837 2316 18-19 -2.672945853701613 2316 20-21 -2.4347661673502756 2316 22-23 -2.7134931101419895 2316 24-25 -2.766846308732525 2316 26-27 -2.663664030474358 2316 28-29 -3.2281733027624675 2316 30-31 -3.3638642364941376 2316 32-33 -3.33866592171416 2316 34-35 -3.145873756074625 2316 36-37 -3.2573045478841003 2316 38-39 -3.473785386412885 2316 40-41 -3.229618566452288 2316 42-43 -3.242972968749463 2316 44-45 -3.3382247273483507 2316 46-47 -3.249049711061602 2316 48-49 -3.049169951735042 2316 50-51 -3.1516070818760262 2316 52-53 -3.168328200492706 2316 54-55 -3.2582921716340145 2316 56-57 -3.0036062620501163 2316 58-59 -3.1149568819756617 2316 60-61 -2.927236920179979 2316 62-63 -2.96332153998193 2316 64-65 -3.147905212663524 2316 66-67 -2.97003026824731 2316 68-69 -3.067728232932243 2316 70-71 -2.956325737157279 2316 72-73 -3.016246655338133 2316 74-75 -3.1976970066892214 2316 76-77 -3.5831557588071483 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76772.0 3 6479.0 4 8590.0 5 10199.0 6 11307.0 7 11920.0 8 13896.0 9 14389.0 10 16798.0 11 18807.0 12 15403.0 13 13512.0 14 13585.0 15 13825.0 16 13874.0 17 14991.0 18 15665.0 19 16636.0 20 17722.0 21 19052.0 22 20758.0 23 23682.0 24 27618.0 25 32207.0 26 38251.0 27 44156.0 28 52123.0 29 60823.0 30 71323.0 31 85528.0 32 104020.0 33 130378.0 34 172534.0 35 231037.0 36 325188.0 37 412950.0 38 324885.0 39 72106.0 40 439.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.552778019572976 25.785359438924605 27.71642656403497 22.94543597746745 2 23.323099642468524 26.0357920099487 27.4775765583709 23.163531789211877 3 23.459452989220836 26.012315048859126 27.255673285888836 23.272558676031203 4 23.173287398636884 26.069248731406397 27.596108402567037 23.161355467389683 5 23.534278923059944 25.996126025429568 27.296791223551033 23.172803827959463 6 23.41285417698181 25.90854222064003 27.49500792436141 23.183595678016758 7 23.37698830810512 25.657341159799596 27.40547046827821 23.560200063817078 8 23.167731918093857 26.119135854050512 27.307190787210768 23.405941440644863 9 23.154050317706652 25.926098661819353 27.62658420946058 23.293266811013417 10-11 23.704537902004947 25.90764841802102 27.147074198807953 23.240739481166084 12-13 23.692508004061544 25.724252185023783 27.07791267678166 23.50532713413301 14-15 23.450108950072615 25.849711467874396 27.339084415223553 23.361095166829436 16-17 23.60447097799 25.743117929088072 27.356478400474103 23.295932692447817 18-19 23.55757515695956 25.828519129537547 27.079434848423023 23.534470865079875 20-21 23.46746781135895 25.987315554486397 27.033134271340025 23.512082362814628 22-23 23.642397888721835 26.11647668699866 26.70261249491603 23.538512929363474 24-25 23.690757074637066 26.278644805480884 26.40152312368617 23.629074996195882 26-27 23.575714860624743 26.314095818946342 26.49669859543351 23.6134907249954 28-29 23.879972609937212 26.151194856513065 26.40846247234956 23.560370061200157 30-31 23.596725406006403 26.079621732357456 26.50451994779272 23.81913291384342 32-33 23.476147079569323 26.41999249950936 26.521054809932515 23.582805610988803 34-35 23.72028573727048 26.021255022573648 26.488506423880924 23.769952816274944 36-37 23.56932832056435 26.02626651567216 26.801907536330788 23.602497627432708 38-39 23.5740346152015 26.16653112462969 26.783534022994537 23.475900237174272 40-41 23.379545470002594 26.217253718524073 26.665157460997886 23.73804335047545 42-43 23.314599700000173 26.435429594580494 26.45054812443755 23.799422580981783 44-45 23.344176242388862 26.10117971291003 26.81868115531685 23.735962889384254 46-47 23.348238828117577 26.09514189433804 26.674565393510306 23.88205388403407 48-49 23.426561808032215 26.167140455603004 26.528481843445416 23.87781589291936 50-51 23.27455326082478 26.401824659489403 26.545678288713187 23.777943790972632 52-53 23.50998081908661 26.209846461345958 26.500096497350256 23.78007622221717 54-55 23.326031281957 26.311444410801805 26.576273334077012 23.786250973164183 56-57 23.387767975003737 26.47098774224653 26.518946865838238 23.622297416911497 58-59 23.342871495211327 26.42064362674274 26.474743951364122 23.761740926681817 60-61 23.249495849907564 26.25989911362475 26.496247708563253 23.994357327904428 62-63 23.467809610727112 26.224564875770135 26.49223347122616 23.815392042276596 64-65 23.315290076415984 26.337454637604157 26.37764289276149 23.969612393218366 66-67 23.27600781838767 26.346462311725755 26.526200499123753 23.851329370762823 68-69 23.359839543975273 26.066466031852155 26.622387074894053 23.951307349278522 70-71 23.29093135186983 25.968236033765546 26.679013876906076 24.061818737458548 72-73 23.463059016216228 25.852330944366003 26.582825332671252 24.101784706746514 74-75 23.240274932371367 25.86913878686908 26.482696558600853 24.4078897221587 76-77 23.257009992720125 25.7319737488878 26.796830764635978 24.214185493756105 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 6288.0 1 3808.5 2 1269.0 3 1249.5 4 1290.0 5 1353.5 6 1508.5 7 1627.0 8 1745.0 9 1836.0 10 1914.0 11 2057.0 12 2240.5 13 2359.0 14 2470.5 15 2735.0 16 3124.0 17 3360.0 18 3659.5 19 4291.0 20 5220.5 21 6599.5 22 7381.0 23 8585.5 24 11221.0 25 14490.5 26 16329.0 27 17802.0 28 22771.0 29 27971.5 30 29676.0 31 33475.0 32 40387.0 33 46298.5 34 53679.5 35 58262.0 36 62292.5 37 70266.0 38 76586.5 39 78964.0 40 83666.0 41 92818.5 42 103492.5 43 109716.0 44 111189.0 45 114777.5 46 119772.5 47 124714.5 48 126777.0 49 128239.5 50 130583.5 51 129451.0 52 127437.0 53 127361.5 54 123204.0 55 112936.5 56 101340.5 57 95930.0 58 91399.0 59 78625.0 60 64814.0 61 59246.0 62 52535.0 63 39440.5 64 28750.0 65 24443.0 66 21333.0 67 16196.5 68 12149.0 69 8604.5 70 7081.0 71 5929.5 72 4017.5 73 2927.0 74 2597.0 75 2263.5 76 1623.5 77 1033.0 78 749.0 79 656.0 80 479.5 81 322.5 82 194.0 83 139.0 84 129.5 85 101.0 86 73.0 87 64.0 88 59.0 89 57.5 90 49.0 91 37.0 92 33.0 93 32.5 94 35.0 95 28.5 96 23.0 97 21.0 98 28.5 99 58.5 100 79.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032641286253200013 2 0.008859777697297145 3 0.012279341019060957 4 0.019429337055476198 5 0.01398912267994286 6 0.013600535938833338 7 0.017447544675817623 8 0.018768739595590007 9 0.017758414068705243 10-11 0.021100260042247153 12-13 0.02003164650419596 14-15 0.008976353719630003 16-17 0.006431110565362622 18-19 0.0121627649967281 20-21 0.010744423391678337 22-23 0.015135453566215956 24-25 0.02166371081685596 26-27 0.013853117320554529 28-29 0.009015212393740956 30-31 0.008684913663797861 32-33 0.01021983129118048 34-35 0.011463308862730956 36-37 0.010336407313513337 38-39 0.016534365834210245 40-41 0.012842791793669768 42-43 0.0165732245083212 44-45 0.018321864843314054 46-47 0.017078387271763577 48-49 0.014630290802773577 50-51 0.02619074635078191 52-53 0.031805824759814534 54-55 0.02467525806045477 56-57 0.015679475003769292 58-59 0.016728659204765003 60-61 0.0207311026381931 62-63 0.025530148890895724 64-65 0.020867107997581437 66-67 0.020167651863584293 68-69 0.029552021661379296 70-71 0.05661708817965764 72-73 0.06780838632361193 74-75 0.07896082579345526 76-77 0.07600756656102288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2573428.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.96371866955355 #Duplication Level Percentage of deduplicated Percentage of total 1 74.56868790086136 38.74866319638102 2 12.830751374983626 13.33467109537273 3 4.851106072996042 7.562445336399872 4 2.4966065892981044 5.1893184973936135 5 1.3931842164273245 3.6197516338645945 6 0.8679460680225747 2.7061023179442163 7 0.5796108506249489 2.108311462578683 8 0.4104039252103923 1.7060891288410658 9 0.30843140199713354 1.4424518342011523 >10 1.5424458890128456 14.194625055932827 >50 0.10116146783299843 3.593344566750989 >100 0.046430120739847605 4.271746654744701 >500 0.002406788459697431 0.7858423737260744 >1k 7.521213936485862E-4 0.4904528745120864 >5k 7.521213936485863E-5 0.24618397135641343 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6299 0.24477078822488915 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.8858674110952395E-5 0.0 2 0.0 0.0 0.0 2.3315204466571436E-4 0.0 3 0.0 0.0 0.0 3.1086939288761916E-4 0.0 4 0.0 0.0 0.0 0.0011269015492176195 0.0 5 0.0 0.0 0.0 0.002642389839544763 0.0 6 0.0 0.0 0.0 0.004468747522759525 0.0 7 0.0 0.0 0.0 0.009714668527738099 3.8858674110952395E-5 8 0.0 0.0 0.0 0.017097816608819053 7.771734822190479E-5 9 0.0 0.0 0.0 0.025219279498008104 7.771734822190479E-5 10 0.0 0.0 0.0 0.03318530769075335 7.771734822190479E-5 11 0.0 0.0 0.0 0.04422117113826383 7.771734822190479E-5 12 0.0 0.0 0.0 0.05735540298776574 7.771734822190479E-5 13 0.0 0.0 0.0 0.07476408898947241 1.1657602233285718E-4 14 0.0 0.0 0.0 0.09586434903171956 1.1657602233285718E-4 15 0.0 0.0 0.0 0.12131678057439338 1.1657602233285718E-4 16 0.0 0.0 0.0 0.14688578813940006 1.1657602233285718E-4 17 0.0 0.0 0.0 0.1727268064231834 1.1657602233285718E-4 18 0.0 0.0 0.0 0.1982569553140791 1.1657602233285718E-4 19 0.0 0.0 0.0 0.2271678088526277 1.1657602233285718E-4 20 0.0 0.0 0.0 0.257943878748502 1.1657602233285718E-4 21 0.0 0.0 0.0 0.29140119715803203 1.5543469644380958E-4 22 0.0 0.0 0.0 0.32878324165276823 1.5543469644380958E-4 23 0.0 0.0 0.0 0.3657766994063949 2.3315204466571436E-4 24 0.0 0.0 0.0 0.40498510158434586 2.3315204466571436E-4 25 0.0 0.0 0.0 0.44827366454394685 2.3315204466571436E-4 26 0.0 0.0 0.0 0.49249483568221064 2.3315204466571436E-4 27 0.0 0.0 0.0 0.5363662787534759 2.3315204466571436E-4 28 0.0 0.0 0.0 0.5800822871282973 2.3315204466571436E-4 29 0.0 0.0 0.0 0.6263629679944417 3.1086939288761916E-4 30 0.0 0.0 0.0 0.6765295162716812 3.1086939288761916E-4 31 0.0 0.0 0.0 0.7299990518483517 3.1086939288761916E-4 32 0.0 0.0 0.0 0.7896082579345527 3.1086939288761916E-4 33 0.0 0.0 0.0 0.8523261579496298 3.1086939288761916E-4 34 0.0 0.0 0.0 0.9183859039382489 3.1086939288761916E-4 35 0.0 0.0 0.0 0.9853393994314199 3.1086939288761916E-4 36 0.0 0.0 0.0 1.0599091950503374 3.1086939288761916E-4 37 0.0 0.0 0.0 1.1334298064682595 3.1086939288761916E-4 38 0.0 0.0 0.0 1.2087379168952852 3.4972806699857156E-4 39 0.0 0.0 0.0 1.2833465711883139 3.4972806699857156E-4 40 0.0 0.0 0.0 1.3611416367584406 3.4972806699857156E-4 41 0.0 0.0 0.0 1.442278548302109 3.4972806699857156E-4 42 0.0 0.0 0.0 1.536083387605948 3.4972806699857156E-4 43 0.0 0.0 0.0 1.6314425738742253 3.4972806699857156E-4 44 0.0 0.0 0.0 1.736943874085461 3.4972806699857156E-4 45 0.0 0.0 0.0 1.8448932707656869 3.4972806699857156E-4 46 0.0 0.0 0.0 1.9543192970621288 3.4972806699857156E-4 47 0.0 0.0 0.0 2.0657659744123404 3.8858674110952397E-4 48 0.0 0.0 0.0 2.185994712111627 4.663040893314287E-4 49 0.0 0.0 0.0 2.3047856788688086 4.663040893314287E-4 50 0.0 0.0 0.0 2.425092133916317 4.663040893314287E-4 51 0.0 0.0 0.0 2.555152116165675 4.663040893314287E-4 52 0.0 0.0 0.0 2.689408835219015 4.663040893314287E-4 53 0.0 0.0 0.0 2.8320201692062104 5.051627634423811E-4 54 3.8858674110952395E-5 0.0 0.0 2.9897863860966774 5.051627634423811E-4 55 3.8858674110952395E-5 0.0 0.0 3.1512441770276847 5.051627634423811E-4 56 3.8858674110952395E-5 0.0 0.0 3.324787015607198 5.051627634423811E-4 57 3.8858674110952395E-5 3.8858674110952395E-5 0.0 3.495959475065943 5.051627634423811E-4 58 3.8858674110952395E-5 3.8858674110952395E-5 0.0 3.674009919842327 5.440214375533335E-4 59 3.8858674110952395E-5 3.8858674110952395E-5 0.0 3.855207917221698 5.440214375533335E-4 60 3.8858674110952395E-5 3.8858674110952395E-5 0.0 4.037455098802065 5.440214375533335E-4 61 3.8858674110952395E-5 3.8858674110952395E-5 0.0 4.2290283621690605 6.217387857752383E-4 62 3.8858674110952395E-5 3.8858674110952395E-5 0.0 4.423127439353268 6.217387857752383E-4 63 3.8858674110952395E-5 3.8858674110952395E-5 0.0 4.6279126519179865 6.217387857752383E-4 64 3.8858674110952395E-5 3.8858674110952395E-5 0.0 4.833552755313146 6.217387857752383E-4 65 3.8858674110952395E-5 3.8858674110952395E-5 0.0 5.061653172344437 6.605974598861907E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCGCG 440 0.0 32.269283 56 TCGCGCT 490 0.0 28.975935 37 CGATCGT 515 0.0 28.257471 1 CTCGCGC 500 0.0 27.686506 36 CGCGGAA 495 0.0 27.252794 59 TCGCGGA 535 0.0 26.541801 58 AATTCGC 535 0.0 26.540255 55 CAATTCG 560 0.0 25.354929 54 CGCGCTT 535 0.0 24.549257 38 CGGTTAC 405 0.0 24.541067 4 AACTCGA 585 0.0 24.271383 13 GATCGTT 635 0.0 22.358948 2 CCTCGCG 605 0.0 22.293407 35 ACTCGAG 640 0.0 22.184269 14 ACGCGAA 115 3.6974638E-5 21.606808 54 CGCTCCG 660 0.0 21.5116 25 CGGCCAA 685 0.0 21.251276 50 ATCGTTC 670 0.0 21.190945 3 AAACTCG 710 0.0 21.001022 12 ACGCTCC 730 0.0 19.935453 24 >>END_MODULE