FastQCFastQC Report
Thu 26 May 2016
SRR936018_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936018_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2573428
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69290.26925175291478914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50190.19503168536287005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35310.13720997828577292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA11950.048.062187100-101
GGTATCA67250.040.094021
GCGTATC3350.035.532795100-101
CGTACAT13800.034.934143100-101
GTATCAA132150.031.4835531
TCGTATC6050.031.480293100-101
CGGTATC9650.031.454807100-101
CCGTACA8150.029.941284100-101
AGTATCA27400.028.129587100-101
TACGCAG8500.028.008204100-101
TCGTACA7700.025.893946100-101
ACGTATC4750.025.68647100-101
GGGTATC12450.025.575762100-101
TAAATCG2250.025.12713896-97
CGAGTAC10850.024.958923100-101
CCGTATC5750.024.842058100-101
CCTGTCG8900.024.74079796-97
ATCAACG173300.023.8597473
TATCAAC173750.023.799342
TCAACGC174700.023.6685414
Produced by FastQC (version 0.11.5)