FastQCFastQC Report
Thu 26 May 2016
SRR936017_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936017_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000710
Sequences flagged as poor quality0
Sequence length77
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70770.353724427828121No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26900.13445226944434724No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG851.18951466E-425.0580316
CGCGGAA1502.1354936E-823.66532759
CTCGAAC6100.020.36769923
CGAACTC6300.020.28355225
CGGTTAC1054.974283E-420.2830434
TACGAAC1054.974283E-420.2830434
CGCGCTT1751.09248504E-720.28253638
CAATTCG1951.5528713E-820.02600954
TTACGGC1601.0371314E-619.9671258
AACGCGA900.00486065119.72356853
ATTCGCG1801.468361E-719.72159856
CTCGCGC1801.4699071E-719.71962536
AATCCGC5800.019.58460635
GCCTCGA6400.019.41296221
TCGAACT6650.019.21695524
TCGCGCT1851.9604886E-719.18762237
CGTACTA950.00664779118.68315122
CGATCGT1902.5979716E-718.6808171
AATTCGC1953.4085497E-718.20409855
TCGCGGA1953.409441E-718.20364258
Produced by FastQC (version 0.11.5)