##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936017_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2000710 Sequences flagged as poor quality 0 Sequence length 77 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.475807588306154 40.0 38.0 41.0 35.0 41.0 2 38.36998865402782 40.0 38.0 41.0 34.0 41.0 3 38.29382319276657 40.0 38.0 41.0 34.0 41.0 4 38.19349530916524 40.0 38.0 41.0 34.0 41.0 5 38.13006882556692 40.0 38.0 41.0 34.0 41.0 6 38.0334346307061 40.0 38.0 41.0 34.0 41.0 7 37.89613837087834 40.0 38.0 41.0 34.0 41.0 8 37.71998640482629 40.0 38.0 41.0 33.0 41.0 9 37.642563889819115 40.0 37.0 41.0 33.0 41.0 10-11 37.83903214358902 40.0 38.0 41.0 34.0 41.0 12-13 38.03257618545416 40.0 38.0 41.0 34.0 41.0 14-15 37.99960788919934 40.0 38.0 41.0 34.0 41.0 16-17 37.863146083140485 40.0 38.0 41.0 33.5 41.0 18-19 37.72327598702461 40.0 38.0 41.0 33.0 41.0 20-21 37.52774714976184 40.0 38.0 41.0 33.0 41.0 22-23 37.27035052556342 40.0 38.0 41.0 33.0 41.0 24-25 37.04501426993417 40.0 37.0 41.0 32.0 41.0 26-27 36.147130268754594 39.0 36.0 40.0 31.0 40.5 28-29 36.210154395189704 39.0 36.0 40.0 31.0 40.5 30-31 36.60327733654553 40.0 36.5 41.0 31.0 41.0 32-33 36.481775969530815 40.0 36.0 41.0 31.0 41.0 34-35 36.259604840281696 39.0 36.0 41.0 30.5 41.0 36-37 36.0096373287483 39.0 35.0 41.0 30.0 41.0 38-39 35.76010541257853 39.0 35.0 40.5 30.0 41.0 40-41 35.421190727291815 38.0 35.0 40.0 29.0 41.0 42-43 35.08226804484408 38.0 35.0 40.0 28.5 41.0 44-45 34.72474746465005 37.0 34.5 40.0 28.0 41.0 46-47 34.34612587531426 36.5 34.0 39.0 28.0 41.0 48-49 33.90417376831225 36.0 34.0 39.0 26.5 40.5 50-51 33.476623548640234 35.5 34.0 38.5 26.0 40.0 52-53 33.038496333801504 35.0 33.5 37.0 26.0 39.5 54-55 32.593082705639496 35.0 33.0 37.0 25.5 39.0 56-57 32.158951072369305 35.0 33.0 36.5 24.5 39.0 58-59 31.742343967891397 35.0 32.5 36.0 23.5 37.0 60-61 31.392962748224377 35.0 32.0 35.5 21.5 37.0 62-63 31.073732824847177 35.0 32.0 35.0 20.0 36.5 64-65 30.73647530126805 34.5 32.0 35.0 18.5 36.0 66-67 30.40097140515117 34.0 31.0 35.0 16.5 36.0 68-69 30.093726976923193 34.0 31.0 35.0 9.0 35.5 70-71 29.743398343588026 34.0 31.0 35.0 2.0 35.0 72-73 29.3486062447831 34.0 30.5 35.0 2.0 35.0 74-75 28.881178931479326 34.0 30.0 35.0 2.0 35.0 76-77 27.215002923961993 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49674861572415807 1101 2 0.5588833292866013 1101 3 0.6238804495564523 1101 4 0.6638239738051652 1101 5 0.6566371007329295 1101 6 0.5105680849921512 1101 7 0.5188252706910745 1101 8 0.7024162999820263 1101 9 0.5956840164937347 1101 10-11 0.48648312935014104 1101 12-13 0.5339576661978853 1101 14-15 0.594265915730297 1101 16-17 0.4929585554537752 1101 18-19 0.5279371091664302 1101 20-21 0.5948890286929682 1101 22-23 0.5283723369118434 1101 24-25 0.505523874631244 1101 26-27 0.3958376422209824 1101 28-29 0.4775282515106838 1101 30-31 0.4820166817402267 1101 32-33 0.5030146728727232 1101 34-35 0.5019257803776256 1101 36-37 0.5975242589881802 1101 38-39 0.6217779796374927 1101 40-41 0.553637806204506 1101 42-43 0.4416318136770414 1101 44-45 0.352144149544678 1101 46-47 0.3616044681450532 1101 48-49 0.46872053790050217 1101 50-51 0.5305311726422701 1101 52-53 0.6478941552348019 1101 54-55 0.5269106271010671 1101 56-57 0.5587745618583355 1101 58-59 0.4793922413572673 1101 60-61 0.4270320253361106 1101 62-63 0.3713003503399186 1101 64-65 0.34307912377894567 1101 66-67 0.44313871413536177 1101 68-69 0.33651125721993225 1101 70-71 0.27155239640164197 1101 72-73 0.4500268668517471 1101 74-75 0.3762316098195484 1101 76-77 0.5407202341904487 1102 1 0.6965583207669752 1102 2 0.649273433948828 1102 3 0.46545989770108065 1102 4 0.6157744971162913 1102 5 0.7394154071078134 1102 6 0.6694643742404836 1102 7 0.5830498187405482 1102 8 0.7038435121609012 1102 9 0.6408790688248516 1102 10-11 0.5319160503777383 1102 12-13 0.6357654682911189 1102 14-15 0.5238567815723485 1102 16-17 0.5869166905632 1102 18-19 0.6073852537905964 1102 20-21 0.6027386956767984 1102 22-23 0.7260412236863445 1102 24-25 0.6943916196360007 1102 26-27 0.6784256536386764 1102 28-29 0.7308584132613944 1102 30-31 0.6960985085718221 1102 32-33 0.7834618660221082 1102 34-35 0.6860361514527895 1102 36-37 0.6924338688835121 1102 38-39 0.7713973897231199 1102 40-41 0.8026863314185846 1102 42-43 0.7265985120595317 1102 44-45 0.7546179839880693 1102 46-47 0.710557845880551 1102 48-49 0.7208613561688182 1102 50-51 0.702510240196986 1102 52-53 0.725201481590652 1102 54-55 0.6384710457499736 1102 56-57 0.7238554372151356 1102 58-59 0.6561286828415689 1102 60-61 0.6051956789992907 1102 62-63 0.6584078669907285 1102 64-65 0.6511190857199551 1102 66-67 0.6429484191781789 1102 68-69 0.47162067682031505 1102 70-71 0.5562812260876591 1102 72-73 0.5894179229887548 1102 74-75 0.6645284699527529 1102 76-77 0.821405296036307 1103 1 0.3044490651225402 1103 2 0.2879235948333587 1103 3 0.4320944213427751 1103 4 0.4203056471987381 1103 5 0.4941543304696765 1103 6 0.3463785819731058 1103 7 0.298736071587129 1103 8 0.38625223267258946 1103 9 0.26344210237218135 1103 10-11 0.3699662474121226 1103 12-13 0.26507489116178107 1103 14-15 0.41840438469997565 1103 16-17 0.3595992882086847 1103 18-19 0.3466171931154065 1103 20-21 0.4006270889660044 1103 22-23 0.3516118102552994 1103 24-25 0.3314960206819606 1103 26-27 0.09934353734308843 1103 28-29 0.1930528685454007 1103 30-31 0.3717892337476272 1103 32-33 0.2887374026021803 1103 34-35 0.29761910851976126 1103 36-37 0.27933582190372874 1103 38-39 0.33053455279034694 1103 40-41 0.41717464524882075 1103 42-43 0.413674342346134 1103 44-45 0.24636737622415694 1103 46-47 0.1232040196747306 1103 48-49 0.18061890024136318 1103 50-51 0.1684359084942244 1103 52-53 0.17584444520701226 1103 54-55 0.23325205408344374 1103 56-57 0.182635543128022 1103 58-59 0.21702538927160475 1103 60-61 0.12949165173752775 1103 62-63 0.061099904256014526 1103 64-65 0.0930938179436609 1103 66-67 0.15311799646793745 1103 68-69 0.1933986000420873 1103 70-71 0.1180959311238432 1103 72-73 0.20138403316272857 1103 74-75 0.238659426116417 1103 76-77 0.49298519052999623 1104 1 0.3403795385633046 1104 2 0.2737740467863361 1104 3 0.21812718040425239 1104 4 0.3204974132174243 1104 5 0.337633014235557 1104 6 0.18443936260982952 1104 7 0.33313504931095395 1104 8 0.4237063937676453 1104 9 0.3520018182122371 1104 10-11 0.3459104987371475 1104 12-13 0.4665930685547224 1104 14-15 0.4999947353623 1104 16-17 0.5605966678228569 1104 18-19 0.6178830094617425 1104 20-21 0.603854005098043 1104 22-23 0.4940897680944474 1104 24-25 0.6253948451446618 1104 26-27 0.46266663542172637 1104 28-29 0.4423387654441697 1104 30-31 0.5503229286611884 1104 32-33 0.7464672284645388 1104 34-35 0.732986085233442 1104 36-37 0.6299468362692267 1104 38-39 0.5605335310495789 1104 40-41 0.6593817525490024 1104 42-43 0.5515218959462445 1104 44-45 0.5108786606593867 1104 46-47 0.4310326509927336 1104 48-49 0.6181870813598636 1104 50-51 0.6309937941244002 1104 52-53 0.5505279398103013 1104 54-55 0.6194586875457659 1104 56-57 0.5102027337380051 1104 58-59 0.5974962199111573 1104 60-61 0.4836808781280659 1104 62-63 0.5216811230544387 1104 64-65 0.48889354872165924 1104 66-67 0.43495269487371147 1104 68-69 0.2934289176339213 1104 70-71 0.3241350883349483 1104 72-73 0.3463469627121434 1104 74-75 0.3576442619663993 1104 76-77 0.43132759683212996 1105 1 0.18669525686934918 1105 2 0.31388182720274926 1105 3 0.2613294926406837 1105 4 0.2622323229731549 1105 5 0.31036817470893396 1105 6 0.10783103709380981 1105 7 0.11154290775971276 1105 8 0.3263814665180149 1105 9 0.1622677197675344 1105 10-11 0.2701184140903692 1105 12-13 0.3353219582699509 1105 14-15 0.21178142253710774 1105 16-17 0.3156384869624773 1105 18-19 0.4414017329990543 1105 20-21 0.5260533622467349 1105 22-23 0.3225298817906648 1105 24-25 0.5178404971654729 1105 26-27 0.3801417960641231 1105 28-29 0.537479068728004 1105 30-31 0.4781056832311492 1105 32-33 0.4763436162148622 1105 34-35 0.5451267350146978 1105 36-37 0.6113613345099793 1105 38-39 0.5688304539834732 1105 40-41 0.7776578011262529 1105 42-43 0.64031960624623 1105 44-45 0.5089665864920363 1105 46-47 0.41147667475738814 1105 48-49 0.46032863259781465 1105 50-51 0.5875971052875357 1105 52-53 0.4902176696831688 1105 54-55 0.4364503011814236 1105 56-57 0.5712602269559497 1105 58-59 0.5991864512193104 1105 60-61 0.27910443600863033 1105 62-63 0.3078886947272892 1105 64-65 0.48786884599109115 1105 66-67 0.6241156359888684 1105 68-69 0.39806902513263154 1105 70-71 0.05632075461004504 1105 72-73 0.14259169130325233 1105 74-75 0.14976200911343085 1105 76-77 0.33181734135366625 1106 1 0.14126701009838172 1106 2 0.14002867309742584 1106 3 -0.05700118601421167 1106 4 0.15614938784158738 1106 5 0.0068406776985483475 1106 6 0.12122798346931063 1106 7 0.25222300164104894 1106 8 0.3374373338535648 1106 9 0.2003105022674987 1106 10-11 -0.004144602517499152 1106 12-13 0.04610466663739743 1106 14-15 0.1217282793482184 1106 16-17 0.19510516950759182 1106 18-19 0.13893437065777903 1106 20-21 0.17457914736167623 1106 22-23 0.20213698938206193 1106 24-25 0.4506868086510494 1106 26-27 0.39096990687586697 1106 28-29 0.3346623804765727 1106 30-31 0.4240013518875827 1106 32-33 0.36709094540987053 1106 34-35 0.3350962682378693 1106 36-37 0.37296848179749276 1106 38-39 0.3285433517510654 1106 40-41 0.28909400697051524 1106 42-43 0.3113382088433596 1106 44-45 0.19793230392508576 1106 46-47 0.23374992304177056 1106 48-49 0.24326351606487862 1106 50-51 0.17300679411952302 1106 52-53 0.2288948458992408 1106 54-55 0.4109316464831423 1106 56-57 0.15088867121514937 1106 58-59 0.17639243937993854 1106 60-61 0.11770370857711754 1106 62-63 0.1683116569514631 1106 64-65 0.3002188070392684 1106 66-67 0.37258758323271834 1106 68-69 0.2670680873116531 1106 70-71 0.048328683911243786 1106 72-73 0.23764803499231135 1106 74-75 0.23592071422579508 1106 76-77 0.3985445446127187 1107 1 0.20690894797584036 1107 2 0.14132498122367565 1107 3 0.14033691060447495 1107 4 0.0733832097884104 1107 5 0.12212610627138076 1107 6 -0.04073878819274768 1107 7 0.03494691945963524 1107 8 0.30450088636681727 1107 9 0.08579853426734019 1107 10-11 0.2490629984279522 1107 12-13 0.34075555287382286 1107 14-15 0.29596452388755523 1107 16-17 0.2049046299525017 1107 18-19 0.32037721369049166 1107 20-21 0.25316451057856426 1107 22-23 0.3394675184703644 1107 24-25 0.20621528271260559 1107 26-27 0.08763257683817471 1107 28-29 0.16238976265159266 1107 30-31 0.3538872001143716 1107 32-33 0.3636452620962842 1107 34-35 0.28765743501346464 1107 36-37 0.2363465487357388 1107 38-39 0.2271411473392959 1107 40-41 0.21055262388149032 1107 42-43 0.18544791837795316 1107 44-45 0.2695562762888599 1107 46-47 0.16556819420505775 1107 48-49 0.3305989591339582 1107 50-51 0.11600731991546809 1107 52-53 0.15496597085685693 1107 54-55 0.1719895774023925 1107 56-57 0.17308831642573352 1107 58-59 0.12107010681155117 1107 60-61 0.11405876334342935 1107 62-63 0.3074895971090079 1107 64-65 0.14809080766825744 1107 66-67 0.2179799891668388 1107 68-69 0.18048251770757417 1107 70-71 0.2508161409753349 1107 72-73 0.3369335421888344 1107 74-75 0.36671663169057567 1107 76-77 0.24393424785742113 1108 1 0.03841904263261853 1108 2 0.06526421146909911 1108 3 0.04541493055375412 1108 4 0.005779980579553978 1108 5 0.04736500872068916 1108 6 -0.0014685062452102216 1108 7 0.047107022669038656 1108 8 0.07971888499746882 1108 9 0.20886027021179387 1108 10-11 0.037655362778018286 1108 12-13 0.13407015278125556 1108 14-15 0.18094586425812054 1108 16-17 0.24423596876169285 1108 18-19 0.07913316382148139 1108 20-21 0.07254013658617708 1108 22-23 0.12076743303736492 1108 24-25 0.14904298910460767 1108 26-27 -0.10311639976026754 1108 28-29 0.044894338420597535 1108 30-31 0.18490161559280693 1108 32-33 0.18113709748884332 1108 34-35 0.15001650901857744 1108 36-37 0.05214860052593906 1108 38-39 0.08283302178960383 1108 40-41 0.11728663642191606 1108 42-43 0.18097410501339795 1108 44-45 0.13288876445850661 1108 46-47 0.2055196563304591 1108 48-49 0.1965667966164304 1108 50-51 0.2134050158811931 1108 52-53 0.30646633836322934 1108 54-55 0.35351454010410777 1108 56-57 0.27042204251160484 1108 58-59 0.2673631629684792 1108 60-61 0.2590872200357097 1108 62-63 0.25237447695981885 1108 64-65 0.10336176263951913 1108 66-67 0.16597014642787755 1108 68-69 0.21500341476803442 1108 70-71 0.2129204540600078 1108 72-73 0.17453242750493914 1108 74-75 0.1760637846930102 1108 76-77 0.059524115401316635 1109 1 -0.04652359718786414 1109 2 0.011073687151210265 1109 3 -0.10740355109667377 1109 4 0.11566487167912243 1109 5 -0.032047703932583715 1109 6 0.003762056795750368 1109 7 -0.1225776666230658 1109 8 0.026375193680969744 1109 9 -0.04591990078855446 1109 10-11 -0.06329202286500646 1109 12-13 -0.016270558235454757 1109 14-15 0.01101927283585269 1109 16-17 -0.02175912204113928 1109 18-19 -0.1586931436612815 1109 20-21 -0.14417444628536913 1109 22-23 -0.20253271589569977 1109 24-25 -0.12201109796470888 1109 26-27 -0.22117236329729906 1109 28-29 -0.20275621493491514 1109 30-31 -0.10057660279976233 1109 32-33 -0.08870701080746812 1109 34-35 -0.07019283989604475 1109 36-37 -0.09875256975607272 1109 38-39 -0.10301522377043426 1109 40-41 -0.09640715410137801 1109 42-43 -0.09609055365883279 1109 44-45 -0.2238928862760119 1109 46-47 -0.36111730018262733 1109 48-49 -0.25105290748822284 1109 50-51 -0.20136374698192583 1109 52-53 -0.16730372330484755 1109 54-55 -0.1818684739425933 1109 56-57 -0.13375569036805857 1109 58-59 -0.20096357413340726 1109 60-61 -0.14121223771498137 1109 62-63 -0.12549900319378793 1109 64-65 -0.17043926530994824 1109 66-67 -0.06499079837373145 1109 68-69 -0.07820396873567148 1109 70-71 -0.07059703463308509 1109 72-73 -0.19730590463194986 1109 74-75 -0.08485124434577784 1109 76-77 -0.029867908592752457 1110 1 -0.2361187039795496 1110 2 -0.1651085572779749 1110 3 -0.24771043991344044 1110 4 -0.21846941667118358 1110 5 -0.0974539227794935 1110 6 -0.15800880134978001 1110 7 -0.34318180493708184 1110 8 -0.06019573912749365 1110 9 -0.1920612245420017 1110 10-11 -0.23780955774369517 1110 12-13 -0.18939536002810797 1110 14-15 -0.16600583164814964 1110 16-17 -0.132146974641266 1110 18-19 -0.04049319371517157 1110 20-21 -0.11157418412931008 1110 22-23 -0.20488961309347786 1110 24-25 -0.20028866393438705 1110 26-27 -0.13804240289000802 1110 28-29 -0.02672475899370852 1110 30-31 -0.11861109169063866 1110 32-33 0.02777460042614166 1110 34-35 0.14214880897548454 1110 36-37 0.06261572143309735 1110 38-39 0.06785993914846955 1110 40-41 0.21565478734858345 1110 42-43 0.11389373369318179 1110 44-45 0.09005919293357323 1110 46-47 0.09618889540175957 1110 48-49 0.1420613867032543 1110 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-0.22660879474092255 2313 68-69 -0.34263144365763765 2313 70-71 -0.396070679460955 2313 72-73 -0.45861720247709314 2313 74-75 -0.5591936496889822 2313 76-77 -0.5502473094451794 2314 1 -0.09660338269715396 2314 2 -0.33558310829729976 2314 3 -0.14616236217661083 2314 4 -0.172171234541878 2314 5 -0.18679017259233177 2314 6 -0.17492363847425452 2314 7 -0.2125558871434876 2314 8 -0.24279190808614715 2314 9 -0.16733020374627472 2314 10-11 -0.16980378430468335 2314 12-13 -0.2745659080491194 2314 14-15 -0.2976786967637679 2314 16-17 -0.6951260989348143 2314 18-19 -0.40305525957961663 2314 20-21 -0.2714711494468318 2314 22-23 -0.12922412845660602 2314 24-25 -0.1484823252414671 2314 26-27 -0.17556882651052064 2314 28-29 -0.2449327143983524 2314 30-31 -0.35307939683102063 2314 32-33 -0.4813652055200919 2314 34-35 -0.45485993953302994 2314 36-37 -0.49453555885118305 2314 38-39 -0.45389205965368973 2314 40-41 -0.3898474172160391 2314 42-43 -0.29580793165753505 2314 44-45 -0.27873968437062757 2314 46-47 -0.16769507223796154 2314 48-49 -0.1766765291113046 2314 50-51 -0.15579228060899908 2314 52-53 -0.14398486386603082 2314 54-55 -0.19672902799627678 2314 56-57 -0.03280465467120308 2314 58-59 -0.13821147819577106 2314 60-61 -0.1334046895466372 2314 62-63 -0.1366243599327639 2314 64-65 -0.06456181168660535 2314 66-67 0.024490271561543153 2314 68-69 0.06631951698459204 2314 70-71 0.1569200668263413 2314 72-73 0.3225352675310269 2314 74-75 0.2979077248377493 2314 76-77 0.44081121259743483 2315 1 -0.6648570078471465 2315 2 -0.5436055076637842 2315 3 -0.5141751860563417 2315 4 -0.5836404051107849 2315 5 -0.5212408143923639 2315 6 -0.3888095083688441 2315 7 -0.3928351317709158 2315 8 -0.4027621311505456 2315 9 -0.31832694323242805 2315 10-11 -0.4848852769493348 2315 12-13 -0.5160951880290767 2315 14-15 -0.7082867805755342 2315 16-17 -0.5091129255700224 2315 18-19 -0.44113650576659325 2315 20-21 -0.47332352129893707 2315 22-23 -0.6435280168314748 2315 24-25 -0.7470068114176129 2315 26-27 -0.6320667740433166 2315 28-29 -0.48808442323890944 2315 30-31 -0.5209872741839874 2315 32-33 -0.6420561152721405 2315 34-35 -0.5811200049049106 2315 36-37 -0.6559855380436943 2315 38-39 -0.6202060426925584 2315 40-41 -0.6722530663491995 2315 42-43 -0.7591809372694982 2315 44-45 -0.7167242666248583 2315 46-47 -0.6829855488709455 2315 48-49 -0.6205702876716686 2315 50-51 -0.5992044871324538 2315 52-53 -0.6229218232247575 2315 54-55 -0.5483175689264286 2315 56-57 -0.6048670104833604 2315 58-59 -0.45335433274129855 2315 60-61 -0.42773803163393254 2315 62-63 -0.593659666004914 2315 64-65 -0.5906630936705497 2315 66-67 -0.5892921164113183 2315 68-69 -0.7060030839923499 2315 70-71 -0.49654857244446404 2315 72-73 -1.978212539601703 2315 74-75 -2.5412632380037046 2315 76-77 -2.102834017638475 2316 1 -1.8880071117903086 2316 2 -1.918886237687076 2316 3 -1.4310338762333643 2316 4 -1.518785160225157 2316 5 -1.8710871260103374 2316 6 -1.571591539420993 2316 7 -1.9460607788762303 2316 8 -1.4974304174937743 2316 9 -1.4904196655413884 2316 10-11 -1.364262160658221 2316 12-13 -1.4302824206920022 2316 14-15 -1.6921209752317168 2316 16-17 -2.6645505652393027 2316 18-19 -2.5697369465857847 2316 20-21 -1.8542268527676953 2316 22-23 -1.7549752270638308 2316 24-25 -1.7924774220802036 2316 26-27 -1.578532045617436 2316 28-29 -1.630741889474777 2316 30-31 -1.7307259969771778 2316 32-33 -2.027737075167053 2316 34-35 -1.9858248592035963 2316 36-37 -1.805114057431112 2316 38-39 -1.773432802977645 2316 40-41 -1.8737570417924516 2316 42-43 -2.1084388290183327 2316 44-45 -1.999080228119972 2316 46-47 -1.7097157781024919 2316 48-49 -1.7089558917525878 2316 50-51 -1.9237544842103524 2316 52-53 -2.851594690302747 2316 54-55 -2.3061352217312248 2316 56-57 -2.1865077887629987 2316 58-59 -2.257116567282708 2316 60-61 -2.009248359159251 2316 62-63 -1.8849309799188312 2316 64-65 -1.8489959399877343 2316 66-67 -2.1016618048044755 2316 68-69 -1.8887781576709344 2316 70-71 -1.8282112664508254 2316 72-73 -1.9767307929669862 2316 74-75 -1.8175142781310534 2316 76-77 -2.0484009183511915 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20739.0 3 1928.0 4 2956.0 5 3846.0 6 4382.0 7 4953.0 8 6257.0 9 6561.0 10 9692.0 11 11970.0 12 8057.0 13 5485.0 14 4838.0 15 4839.0 16 4744.0 17 4923.0 18 5205.0 19 5452.0 20 5861.0 21 6327.0 22 7123.0 23 8062.0 24 9478.0 25 10982.0 26 13138.0 27 15896.0 28 19727.0 29 24394.0 30 30998.0 31 40190.0 32 53908.0 33 76315.0 34 115346.0 35 172393.0 36 272975.0 37 421097.0 38 441635.0 39 136686.0 40 1352.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.630254343910877 27.073582933570066 29.801520475027232 21.49464224749183 2 21.259633908960122 27.3851953975184 29.766916639256664 21.588254054264812 3 21.4706577774623 27.517059664210418 29.278787730338586 21.733494827988697 4 21.150151790922937 27.538017715022978 29.59390616806527 21.717924325988815 5 21.31911692297695 27.663315012959227 29.56843397042247 21.449134093641355 6 21.597821398026245 27.458112748050663 29.47830820439789 21.465757649525205 7 21.45616182864044 27.24059792935244 29.660919089179018 21.642321152828107 8 21.30237798985896 27.605958247079187 29.317976971456023 21.773686791605822 9 21.266788043483693 27.30603454740145 29.61469359352364 21.81248381559122 10-11 21.673774578786844 27.53798954484534 29.257086124299057 21.53114975206876 12-13 21.64079461273402 27.293329754086766 29.268064072512733 21.79781156066648 14-15 21.37521893694616 27.603950062382033 29.37961367126268 21.64121732940913 16-17 21.6179639479418 27.40893138932334 29.346818240663957 21.6262864220709 18-19 21.475682893068115 27.358188743080834 29.211121847729203 21.955006516121838 20-21 21.45296596538072 27.67992725096692 29.05427006878713 21.812836714865234 22-23 21.532969413049187 27.64870986101658 28.7120256415576 22.106295084376633 24-25 21.546664973656274 27.841089312213697 28.602943988434205 22.009301725695824 26-27 21.71322658214559 27.83056456813762 28.497992791045295 21.958216058671493 28-29 21.714771145909978 27.85295334943536 28.475199142646918 21.95707636200774 30-31 21.572681170268574 27.776871097857196 28.515080725198423 22.135367006675803 32-33 21.567880591941655 28.023528706071126 28.35843911753606 22.050151584451157 34-35 21.608452029941315 27.78339161420305 28.556018242316284 22.05213811353935 36-37 21.66793966011075 27.873107880195985 28.647876017103435 21.811076442589826 38-39 21.585092258596006 27.778930288173143 28.705365355237582 21.930612097993265 40-41 21.47619471317986 27.761754182158455 28.677190253343877 22.084860851317806 42-43 21.442941370102957 27.97892779919373 28.405555634704193 22.172575195999112 44-45 21.52951329918652 27.85739618926362 28.56825076187984 22.044839749670018 46-47 21.448732619542902 27.755914467129294 28.686046691832807 22.109306221495 48-49 21.577731495738632 27.792811663873746 28.444240252442636 22.185216587944982 50-51 21.40309414374574 27.980361991719416 28.421089734968387 22.195454129566457 52-53 21.281679232443455 27.891449488477306 28.45629694666374 22.370574332415504 54-55 21.289092721156607 27.89513798048573 28.468897428468797 22.346871869888865 56-57 21.310414125457417 28.04050270951229 28.396088704033275 22.25299446099702 58-59 21.407588277329523 28.03601453598742 28.317731150219117 22.238666036463943 60-61 21.391506356659836 27.84122522987308 28.334015716304656 22.433252697162438 62-63 21.338910669630316 27.83518761267409 28.44566496586906 22.380236751826533 64-65 21.39552055808424 27.758406796277406 28.380422357664948 22.465650287973407 66-67 21.40436675459103 27.808313552017694 28.491443872724982 22.295875820666293 68-69 21.431005400345857 27.660940981702424 28.48393058976797 22.42412302818375 70-71 21.321619784216463 27.506462729696 28.532923479130112 22.638994006957418 72-73 21.41590578735128 27.422392310608828 28.481929470564047 22.679772431475843 74-75 21.232870374191336 27.354555274360376 28.44553921322206 22.96703513822623 76-77 21.10577875420936 27.30499380726281 28.773217762250912 22.816009676276913 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7637.0 1 4574.0 2 1437.0 3 1355.0 4 1347.0 5 1472.5 6 1633.5 7 1712.5 8 1823.5 9 1891.0 10 1941.5 11 2076.5 12 2175.5 13 2190.0 14 2362.5 15 2680.5 16 3066.5 17 3307.0 18 3643.5 19 4317.5 20 5332.5 21 7117.5 22 8225.0 23 9606.5 24 12737.5 25 16951.0 26 19415.0 27 22638.0 28 29240.5 29 35664.5 30 38709.0 31 43112.5 32 52155.0 33 60571.0 34 67640.5 35 70933.0 36 74870.0 37 81637.5 38 86194.5 39 87921.0 40 89218.5 41 91699.0 42 94703.5 43 96525.0 44 95711.0 45 94584.0 46 94119.5 47 93493.0 48 93018.0 49 91495.5 50 87854.5 51 82115.0 52 78494.0 53 76045.5 54 69431.5 55 59927.0 56 50162.0 57 45736.0 58 42647.5 59 35396.0 60 28369.0 61 25505.0 62 22155.5 63 15711.5 64 10991.0 65 9365.0 66 7996.0 67 5933.5 68 4411.0 69 3047.5 70 2513.0 71 2123.0 72 1478.0 73 1125.0 74 1027.0 75 927.5 76 659.0 77 400.0 78 310.0 79 265.0 80 192.0 81 138.5 82 97.0 83 81.0 84 75.0 85 60.0 86 46.5 87 42.0 88 43.0 89 38.0 90 26.5 91 21.0 92 20.5 93 21.0 94 21.5 95 25.5 96 30.0 97 23.0 98 24.5 99 59.5 100 86.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007547320701151091 2 0.011196025410979102 3 0.0115459012050722 4 0.013045368894042616 5 0.010246362541297838 6 0.00804714326414123 7 0.01364515596963078 8 0.016144268784581473 9 0.016394180066076545 10-11 0.017843665498747944 12-13 0.014444872070415001 14-15 0.006697622344067856 16-17 0.005148172398798427 18-19 0.014344907557816975 20-21 0.020417751698147156 22-23 0.018168550164691533 24-25 0.016419171194226048 26-27 0.020992547645585814 28-29 0.006697622344067856 30-31 0.006322755421825252 32-33 0.008746894852327424 34-35 0.009821513362756221 36-37 0.008796877108626438 38-39 0.012295635049557408 40-41 0.009396664184214604 42-43 0.012720484228099025 44-45 0.011520910076922693 46-47 0.02014284928850258 48-49 0.020692654107791735 50-51 0.030439194086099436 52-53 0.03503756166560871 54-55 0.024066456407975167 56-57 0.017493789704654848 58-59 0.014769756736358592 60-61 0.018343488061738085 62-63 0.023591624973134537 64-65 0.021917219387117576 66-67 0.025291021687301008 68-69 0.03726177207091482 70-71 0.048707708763389 72-73 0.0743735973729326 74-75 0.07524828685816536 76-77 0.07967171654062807 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2000710.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.91528023105551 #Duplication Level Percentage of deduplicated Percentage of total 1 75.3332748804738 44.38281000306249 2 13.826740969777603 16.292126378333275 3 4.868512789367201 8.604893858821391 4 2.1864044801014546 5.152505305744497 5 1.1482811263543744 3.3825652171600016 6 0.6780269901722458 2.3967690078130195 7 0.41498879402244954 1.7114426764806272 8 0.27662995998710543 1.3038185290356794 9 0.20102760436653383 1.065923788088889 >10 0.9785545877731372 10.285974154887226 >50 0.06301159614662274 2.5215860455468557 >100 0.024121822194714305 2.3202515882867205 >500 2.5463955769638465E-4 0.09091309505154253 >1k 8.48798525654595E-5 0.13451937606636102 >5k 8.48798525654595E-5 0.3539009756214264 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7077 0.353724427828121 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2690 0.13445226944434724 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 4.998225629901385E-5 0.0 0.0 4.998225629901385E-5 2 0.0 4.998225629901385E-5 0.0 1.999290251960554E-4 4.998225629901385E-5 3 0.0 4.998225629901385E-5 0.0 2.4991128149506924E-4 4.998225629901385E-5 4 0.0 4.998225629901385E-5 0.0 6.99751588186194E-4 4.998225629901385E-5 5 0.0 4.998225629901385E-5 0.0 0.0014494854326714017 4.998225629901385E-5 6 0.0 4.998225629901385E-5 0.0 0.0031488821468378726 4.998225629901385E-5 7 0.0 4.998225629901385E-5 0.0 0.006597657831469829 4.998225629901385E-5 8 0.0 4.998225629901385E-5 0.0 0.010546256079091923 4.998225629901385E-5 9 0.0 4.998225629901385E-5 0.0 0.01454483658301303 4.998225629901385E-5 10 0.0 4.998225629901385E-5 0.0 0.020242813801100608 4.998225629901385E-5 11 4.998225629901385E-5 9.99645125980277E-5 0.0 0.0263406490695803 4.998225629901385E-5 12 4.998225629901385E-5 9.99645125980277E-5 0.0 0.03493759715301068 4.998225629901385E-5 13 4.998225629901385E-5 9.99645125980277E-5 0.0 0.04683337415217598 4.998225629901385E-5 14 4.998225629901385E-5 9.99645125980277E-5 0.0 0.06032858335290972 9.99645125980277E-5 15 4.998225629901385E-5 9.99645125980277E-5 0.0 0.07547320701151092 9.99645125980277E-5 16 4.998225629901385E-5 9.99645125980277E-5 0.0 0.09096770646420521 9.99645125980277E-5 17 4.998225629901385E-5 9.99645125980277E-5 0.0 0.10731190427398274 9.99645125980277E-5 18 4.998225629901385E-5 9.99645125980277E-5 0.0 0.12265645695777999 9.99645125980277E-5 19 4.998225629901385E-5 1.999290251960554E-4 0.0 0.1397004063557437 9.99645125980277E-5 20 4.998225629901385E-5 1.999290251960554E-4 0.0 0.15984325564424628 9.99645125980277E-5 21 4.998225629901385E-5 1.999290251960554E-4 0.0 0.18118567908392522 9.99645125980277E-5 22 4.998225629901385E-5 1.999290251960554E-4 0.0 0.20542707338894692 9.99645125980277E-5 23 4.998225629901385E-5 1.999290251960554E-4 0.0 0.23011830800065977 9.99645125980277E-5 24 4.998225629901385E-5 1.999290251960554E-4 0.0 0.25540932968796076 9.99645125980277E-5 25 4.998225629901385E-5 1.999290251960554E-4 0.0 0.28194990778273715 9.99645125980277E-5 26 4.998225629901385E-5 1.999290251960554E-4 0.0 0.31183929704954744 9.99645125980277E-5 27 4.998225629901385E-5 1.999290251960554E-4 0.0 0.3406790589340784 9.99645125980277E-5 28 4.998225629901385E-5 1.999290251960554E-4 0.0 0.3707183949697857 9.99645125980277E-5 29 4.998225629901385E-5 2.998935377940831E-4 0.0 0.4035067551019388 9.99645125980277E-5 30 4.998225629901385E-5 3.49875794093097E-4 0.0 0.4397938731750229 9.99645125980277E-5 31 4.998225629901385E-5 3.49875794093097E-4 0.0 0.479879642726832 9.99645125980277E-5 32 4.998225629901385E-5 3.49875794093097E-4 0.0 0.5241639218077583 9.99645125980277E-5 33 4.998225629901385E-5 3.49875794093097E-4 0.0 0.566598857405621 9.99645125980277E-5 34 4.998225629901385E-5 3.998580503921108E-4 0.0 0.6121826751503217 9.99645125980277E-5 35 4.998225629901385E-5 3.998580503921108E-4 0.0 0.6591160138150957 9.99645125980277E-5 36 4.998225629901385E-5 3.998580503921108E-4 0.0 0.7094981281645016 9.99645125980277E-5 37 4.998225629901385E-5 3.998580503921108E-4 0.0 0.7603300828205987 9.99645125980277E-5 38 4.998225629901385E-5 3.998580503921108E-4 0.0 0.8141109905983376 9.99645125980277E-5 39 4.998225629901385E-5 3.998580503921108E-4 0.0 0.8714906208296055 9.99645125980277E-5 40 4.998225629901385E-5 3.998580503921108E-4 0.0 0.9333686541277847 9.99645125980277E-5 41 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.0025440968456198 9.99645125980277E-5 42 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.0709198234626707 9.99645125980277E-5 43 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.1499917529277106 9.99645125980277E-5 44 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.2288137711112554 9.99645125980277E-5 45 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.3152830745085495 9.99645125980277E-5 46 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.4100994147077788 9.99645125980277E-5 47 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.504615861369214 9.99645125980277E-5 48 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.6005817934633204 9.99645125980277E-5 49 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.7012960399058334 1.4994676889704155E-4 50 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.8038096475751109 1.4994676889704155E-4 51 4.998225629901385E-5 4.4984030669112466E-4 0.0 1.9149701855841177 1.4994676889704155E-4 52 4.998225629901385E-5 4.4984030669112466E-4 0.0 2.0370268554663093 1.4994676889704155E-4 53 4.998225629901385E-5 4.4984030669112466E-4 0.0 2.1631820703650204 1.4994676889704155E-4 54 4.998225629901385E-5 4.4984030669112466E-4 0.0 2.3014329912880926 1.4994676889704155E-4 55 4.998225629901385E-5 4.4984030669112466E-4 0.0 2.4434825636898903 1.4994676889704155E-4 56 4.998225629901385E-5 4.4984030669112466E-4 0.0 2.5956285518640883 1.4994676889704155E-4 57 4.998225629901385E-5 4.4984030669112466E-4 0.0 2.7524728721303937 1.4994676889704155E-4 58 4.998225629901385E-5 4.998225629901385E-4 0.0 2.908067635989224 1.4994676889704155E-4 59 4.998225629901385E-5 4.998225629901385E-4 0.0 3.0695603060913377 1.4994676889704155E-4 60 4.998225629901385E-5 4.998225629901385E-4 0.0 3.23315223095801 1.4994676889704155E-4 61 4.998225629901385E-5 4.998225629901385E-4 0.0 3.4044914055510294 1.4994676889704155E-4 62 4.998225629901385E-5 4.998225629901385E-4 0.0 3.5961233762014486 1.4994676889704155E-4 63 4.998225629901385E-5 4.998225629901385E-4 0.0 3.7877053645955687 1.4994676889704155E-4 64 4.998225629901385E-5 4.998225629901385E-4 0.0 3.991483023526648 1.4994676889704155E-4 65 4.998225629901385E-5 4.998225629901385E-4 0.0 4.205906903049417 1.4994676889704155E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAACGG 85 1.18951466E-4 25.058031 6 CGCGGAA 150 2.1354936E-8 23.665327 59 CTCGAAC 610 0.0 20.367699 23 CGAACTC 630 0.0 20.283552 25 CGGTTAC 105 4.974283E-4 20.283043 4 TACGAAC 105 4.974283E-4 20.283043 4 CGCGCTT 175 1.09248504E-7 20.282536 38 CAATTCG 195 1.5528713E-8 20.026009 54 TTACGGC 160 1.0371314E-6 19.96712 58 AACGCGA 90 0.004860651 19.723568 53 ATTCGCG 180 1.468361E-7 19.721598 56 CTCGCGC 180 1.4699071E-7 19.719625 36 AATCCGC 580 0.0 19.584606 35 GCCTCGA 640 0.0 19.412962 21 TCGAACT 665 0.0 19.216955 24 TCGCGCT 185 1.9604886E-7 19.187622 37 CGTACTA 95 0.006647791 18.683151 22 CGATCGT 190 2.5979716E-7 18.680817 1 AATTCGC 195 3.4085497E-7 18.204098 55 TCGCGGA 195 3.409441E-7 18.203642 58 >>END_MODULE