FastQCFastQC Report
Thu 26 May 2016
SRR936017_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936017_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2000710
Sequences flagged as poor quality0
Sequence length125
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75860.37916539628431906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55660.2782012385603111No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39400.19693008981811455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA8400.047.82651100-101
GGTATCA63000.042.8785551
CGGTATC5850.039.67829100-101
CGTACAT12100.033.939697100-101
ACGTATC3600.033.065247100-101
GCGTATC2650.032.566147100-101
CCGCGTA751.4760553E-731.74263498-99
TACGCAG7000.031.034094100-101
GTATCAA119500.030.7712671
CCGTATC4100.029.032898100-101
CCGTACA5350.028.924362100-101
CGAGTAC7750.028.41478100-101
TCGAGTA3700.028.150139100-101
AGTATCA23200.028.091206100-101
AGGTATC18400.027.171003100-101
TCGGTAT2600.026.325018100-101
CCTGTCG6450.026.29544396-97
CCGGTAT2500.026.187672100-101
CTCGGTA2800.025.50747598-99
CGCGTAC701.05445986E-425.50747598-99
Produced by FastQC (version 0.11.5)