FastQCFastQC Report
Thu 26 May 2016
SRR936016_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936016_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022674
Sequences flagged as poor quality0
Sequence length77
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72690.3593757570424102No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26880.13289338766405262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCT1751.6916601E-1024.34046637
GACGTAT1858.480129E-921.10707939
CGATCGT2051.2223609E-920.7789611
CTCGCGC2152.2118911E-919.81151636
TACGTAG1851.960343E-719.1877782
CGCGGAA2052.755769E-819.05020759
CGCCCAA1159.16442E-418.52266958
GTCGGGT2701.0004442E-1018.41242866
CGCGCTT2356.672053E-918.1258838
CAATTCG2559.349606E-1018.09760554
TCGCGGA2206.187656E-817.7508958
ATTCGCG2408.651114E-917.74957356
ATACGTA2004.4464832E-717.7486951
CGGCGCA1000.00893768417.74825743
ATTCCGC1452.1903451E-417.1375254
CTCCGCG2902.8194336E-1017.13370527
CGCTCCG2602.329034E-816.3817925
TATTCCG1350.002681214415.77817853
CGCGCCC2956.49743E-915.63982530
ACTCGAG2754.64388E-815.48823814
Produced by FastQC (version 0.11.5)