##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936016_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2022674 Sequences flagged as poor quality 0 Sequence length 77 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.445384673951416 40.0 38.0 41.0 34.0 41.0 2 38.3403885154009 40.0 38.0 41.0 34.0 41.0 3 38.22772972807284 40.0 38.0 41.0 34.0 41.0 4 38.139909347724846 40.0 38.0 41.0 34.0 41.0 5 38.077704068970085 40.0 38.0 41.0 34.0 41.0 6 37.98822004930108 40.0 38.0 41.0 34.0 41.0 7 37.857699263450264 40.0 38.0 41.0 34.0 41.0 8 37.73557429422635 40.0 38.0 41.0 33.0 41.0 9 37.89296594508062 40.0 38.0 41.0 34.0 41.0 10-11 38.11350667482748 40.0 38.0 41.0 34.0 41.0 12-13 38.082733302549 40.0 38.0 41.0 34.0 41.0 14-15 37.96045927321951 40.0 38.0 41.0 34.0 41.0 16-17 37.82766698934183 40.0 38.0 41.0 33.0 41.0 18-19 37.683725602840596 40.0 38.0 41.0 33.0 41.0 20-21 37.48816294667356 40.0 38.0 41.0 33.0 41.0 22-23 37.221732963394004 40.0 37.5 41.0 32.5 41.0 24-25 37.00894212314985 40.0 37.0 41.0 32.0 41.0 26-27 36.09249463828576 39.0 36.0 40.0 30.5 40.5 28-29 36.13925872384773 39.0 36.0 40.0 30.5 40.5 30-31 36.55486227637276 40.0 36.0 41.0 31.0 41.0 32-33 36.461357094618315 40.0 36.0 41.0 31.0 41.0 34-35 36.25530906117348 39.0 36.0 41.0 30.5 41.0 36-37 35.98551298924097 39.0 35.0 41.0 30.0 41.0 38-39 35.71405673875276 39.0 35.0 40.5 29.0 41.0 40-41 35.392218419775006 38.0 35.0 40.0 29.0 41.0 42-43 35.04733362865197 38.0 35.0 40.0 28.5 41.0 44-45 34.69873914432084 37.0 34.5 40.0 28.0 41.0 46-47 34.295704596983995 36.5 34.0 39.0 28.0 41.0 48-49 33.885752721397516 36.0 34.0 39.0 26.5 40.5 50-51 33.44139268117354 35.5 34.0 38.5 26.0 40.0 52-53 32.97423534390614 35.0 33.5 37.0 26.0 39.0 54-55 32.52786361025059 35.0 33.0 37.0 25.0 39.0 56-57 32.10202682191989 35.0 33.0 36.5 24.5 39.0 58-59 31.693946973165225 35.0 32.5 36.0 23.0 37.0 60-61 31.335111589905246 35.0 32.0 35.5 21.5 37.0 62-63 30.9930648735288 35.0 32.0 35.0 20.0 36.5 64-65 30.65423592729229 34.0 31.5 35.0 18.0 36.0 66-67 30.344648223094772 34.0 31.0 35.0 15.0 36.0 68-69 30.036796339894615 34.0 31.0 35.0 7.5 35.5 70-71 29.655526298355547 34.0 31.0 35.0 2.0 35.0 72-73 29.263163762425386 34.0 30.5 35.0 2.0 35.0 74-75 28.80920108727358 34.0 30.0 35.0 2.0 35.0 76-77 27.081470864805695 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6901552789271079 1101 2 0.6925605542781454 1101 3 0.7923478165080624 1101 4 0.7478613874978208 1101 5 0.7298236149198303 1101 6 0.8655998919802599 1101 7 0.8687493337855159 1101 8 0.865927329302778 1101 9 0.7936347535306751 1101 10-11 0.8146992414936562 1101 12-13 0.8579853764030361 1101 14-15 0.8536811803164355 1101 16-17 0.8303903073101253 1101 18-19 0.8066570474811812 1101 20-21 0.8674089010382744 1101 22-23 0.8586069779255396 1101 24-25 0.9257025409447621 1101 26-27 0.9608137897175695 1101 28-29 0.9636942671833708 1101 30-31 1.037421233444654 1101 32-33 1.038704479030045 1101 34-35 0.9764131919785157 1101 36-37 0.9071332209428462 1101 38-39 0.944522841097232 1101 40-41 0.9324099846265668 1101 42-43 0.9769947125564258 1101 44-45 0.9122766190880114 1101 46-47 0.8588513680922816 1101 48-49 0.7697435427050507 1101 50-51 0.8357880827994748 1101 52-53 0.9317453960953586 1101 54-55 0.90533832468779 1101 56-57 0.8751348546225017 1101 58-59 0.9333173555558218 1101 60-61 0.9004539425303157 1101 62-63 0.8818610708613122 1101 64-65 0.9370075705283867 1101 66-67 1.1371140422172274 1101 68-69 0.912817558471648 1101 70-71 0.9681228282126035 1101 72-73 0.8719082251418548 1101 74-75 0.9397689366251036 1101 76-77 0.9707166238087126 1102 1 0.6332366774093217 1102 2 0.6374617177150981 1102 3 0.6168691282209124 1102 4 0.4909435859692479 1102 5 0.6215107585035824 1102 6 0.5787639672432405 1102 7 0.567969596923021 1102 8 0.5896792619083158 1102 9 0.5824674934684921 1102 10-11 0.6619589836781827 1102 12-13 0.7369082709156132 1102 14-15 0.7231880964337662 1102 16-17 0.6617492111059491 1102 18-19 0.6584787281995901 1102 20-21 0.5935458541403733 1102 22-23 0.5964442996963584 1102 24-25 0.6085956556480596 1102 26-27 0.2956136997819385 1102 28-29 0.5573159531227674 1102 30-31 0.6753727431506249 1102 32-33 0.5669909400124666 1102 34-35 0.5750072319746025 1102 36-37 0.5510306505353739 1102 38-39 0.5885233219328541 1102 40-41 0.5640233692041292 1102 42-43 0.5187426763733356 1102 44-45 0.3914131107226453 1102 46-47 0.45737098803845555 1102 48-49 0.384231158029813 1102 50-51 0.4023012918846547 1102 52-53 0.48332385903817254 1102 54-55 0.5539933743881775 1102 56-57 0.3863813346699274 1102 58-59 0.428361317068763 1102 60-61 0.384847100180405 1102 62-63 0.372358356792617 1102 64-65 0.3231751042710904 1102 66-67 0.4018087915932327 1102 68-69 0.38050452016311453 1102 70-71 0.40654313160219857 1102 72-73 0.5448118954924013 1102 74-75 0.40271785689228423 1102 76-77 0.5568242004645221 1103 1 0.260412021788504 1103 2 0.14040907920322354 1103 3 0.16289612428154499 1103 4 0.17182450555116446 1103 5 0.11639869119348134 1103 6 0.12036361278148888 1103 7 0.19145833373816146 1103 8 0.25820897694894995 1103 9 0.20758505219867374 1103 10-11 0.2368197843049913 1103 12-13 0.1298070820915882 1103 14-15 0.21557238635337939 1103 16-17 0.18325149941149022 1103 18-19 0.09526720117850829 1103 20-21 0.1671089646278361 1103 22-23 0.08336839022267384 1103 24-25 0.20567842426467564 1103 26-27 0.13134924428147343 1103 28-29 0.03445160873373965 1103 30-31 0.13022922847925855 1103 32-33 0.09865053783009614 1103 34-35 0.18300655025158363 1103 36-37 0.26316797196264474 1103 38-39 0.2237011066580692 1103 40-41 0.31036199480089977 1103 42-43 0.2300513664600814 1103 44-45 0.19691644171371792 1103 46-47 0.13614012728401548 1103 48-49 0.16016464598397562 1103 50-51 0.19869930094549915 1103 52-53 0.28782876945403757 1103 54-55 0.34622074826193483 1103 56-57 0.31648088162777555 1103 58-59 0.29880111823145583 1103 60-61 0.10373696513167019 1103 62-63 0.14510220214669545 1103 64-65 0.08572956406119303 1103 66-67 0.1997772441546779 1103 68-69 0.20470337799905636 1103 70-71 0.12990097674158463 1103 72-73 0.057630127349224125 1103 74-75 0.11303400701333999 1103 76-77 0.022593065566763215 1104 1 0.31743967699053854 1104 2 0.09890698600869996 1104 3 0.2713881583110691 1104 4 0.3221032695075863 1104 5 0.26134223109706767 1104 6 0.27009196474337926 1104 7 0.2390788306693139 1104 8 0.26335772964849724 1104 9 0.1204990721866892 1104 10-11 0.23996021909616871 1104 12-13 0.3908489725596098 1104 14-15 0.4318337171672795 1104 16-17 0.5316232829949783 1104 18-19 0.40128105235636014 1104 20-21 0.359336067017324 1104 22-23 0.49966718677513455 1104 24-25 0.42879932886079075 1104 26-27 0.2755749766423534 1104 28-29 0.3321280470708814 1104 30-31 0.44520527869877213 1104 32-33 0.3902567977805944 1104 34-35 0.4461559956814867 1104 36-37 0.3220226177570922 1104 38-39 0.2699432159191417 1104 40-41 0.42711278076694015 1104 42-43 0.32702311454971067 1104 44-45 0.3447680773889843 1104 46-47 0.28646025787397633 1104 48-49 0.13265454866735382 1104 50-51 0.15598278627084738 1104 52-53 0.18175679065433314 1104 54-55 0.06073267771317603 1104 56-57 0.19791253580442714 1104 58-59 0.2543221277659278 1104 60-61 0.24158066184265792 1104 62-63 0.07445549377332128 1104 64-65 0.16891786411974863 1104 66-67 0.1679245076554139 1104 68-69 -0.1057424900100905 1104 70-71 -0.02846914360316788 1104 72-73 -0.1114318745900782 1104 74-75 -0.23106715391015342 1104 76-77 -0.05309737141063664 1105 1 0.12142382087832715 1105 2 0.312354119135378 1105 3 0.12715222979048235 1105 4 0.2110755187211879 1105 5 0.09930594748452393 1105 6 0.29912114263167666 1105 7 0.20226879623497496 1105 8 0.050792498875416925 1105 9 0.05828481092343907 1105 10-11 0.22983841469719124 1105 12-13 0.32432545494768306 1105 14-15 0.2653443230898844 1105 16-17 0.2566652953036268 1105 18-19 0.16100107965519328 1105 20-21 0.09105612625975823 1105 22-23 0.269139508725182 1105 24-25 0.3339085997135953 1105 26-27 0.23567256433940997 1105 28-29 0.24494543802988744 1105 30-31 0.42558931846769354 1105 32-33 0.3581826178353964 1105 34-35 0.20885390031084938 1105 36-37 0.36085157402587953 1105 38-39 0.4118565425730907 1105 40-41 0.4401767417819329 1105 42-43 0.47231760648028853 1105 44-45 0.3058958461760355 1105 46-47 0.329632680360028 1105 48-49 0.20375452652267256 1105 50-51 0.2933442578865808 1105 52-53 0.29324340693356277 1105 54-55 0.3210665401303672 1105 56-57 0.3211672389598945 1105 58-59 0.28607989340609663 1105 60-61 0.39377766924537383 1105 62-63 0.2619445671197127 1105 64-65 0.46156990810004217 1105 66-67 0.3908626670047681 1105 68-69 0.3465908604461667 1105 70-71 0.2807926009576782 1105 72-73 -0.021326857847746794 1105 74-75 -0.1956803363430737 1105 76-77 -0.3180344576277072 1106 1 -0.05910045684001375 1106 2 -0.031020586526565808 1106 3 -0.04675102528964459 1106 4 0.06760694778080989 1106 5 0.08908840023851639 1106 6 0.017783403206259152 1106 7 -0.27184952379629124 1106 8 0.003747965787901819 1106 9 0.04054413875745411 1106 10-11 -0.22579443258920406 1106 12-13 0.20264614098491052 1106 14-15 0.1821883419140633 1106 16-17 0.07923368342421355 1106 18-19 0.07882680461647595 1106 20-21 0.16076410119821816 1106 22-23 0.0886632175562525 1106 24-25 -0.008500993290951442 1106 26-27 -0.018979271942150433 1106 28-29 0.044856775031576035 1106 30-31 0.20737687573481622 1106 32-33 0.07722327701809917 1106 34-35 -0.034321750865508704 1106 36-37 0.05464222664967622 1106 38-39 -0.02096598814334527 1106 40-41 -0.15131152068565967 1106 42-43 -0.08551504289523137 1106 44-45 -0.01865938690293234 1106 46-47 -0.013774988025112123 1106 48-49 -0.10386515451028089 1106 50-51 -0.13801513154511724 1106 52-53 -0.03416467135536294 1106 54-55 -0.034805073874807135 1106 56-57 -0.06776385616715785 1106 58-59 -0.1579389756226881 1106 60-61 -0.3085517862216527 1106 62-63 -0.3039198870231985 1106 64-65 -0.1555233268503997 1106 66-67 -0.20704009654958355 1106 68-69 -0.003049453457624196 1106 70-71 -0.2258165371612897 1106 72-73 -0.2878855248800676 1106 74-75 -0.2781965716054273 1106 76-77 -0.46791752965484434 1107 1 -0.0645997252643511 1107 2 -0.10706519626538125 1107 3 0.026930153092330045 1107 4 0.0030052942089255907 1107 5 0.03961450207761885 1107 6 -0.20683096600770767 1107 7 -0.21228679112673632 1107 8 -0.10991726644306254 1107 9 -0.17051039357926356 1107 10-11 -0.0681094781012348 1107 12-13 0.0432103179611687 1107 14-15 -0.0893893264750929 1107 16-17 -0.03487136913661004 1107 18-19 0.09064171799172271 1107 20-21 -0.01654182036951113 1107 22-23 -0.07893412732435223 1107 24-25 -0.044111296999176375 1107 26-27 -0.17876473025489048 1107 28-29 -0.15056512270396638 1107 30-31 0.18582545523069172 1107 32-33 0.2630712026051185 1107 34-35 0.18523978831047572 1107 36-37 0.34908068119215585 1107 38-39 0.25245845451534876 1107 40-41 0.2766734807792872 1107 42-43 0.33037271217476416 1107 44-45 0.37939966051869334 1107 46-47 0.27481030032696907 1107 48-49 0.2822035449541218 1107 50-51 0.3762961351688361 1107 52-53 0.3003453299861931 1107 54-55 0.1923462543803538 1107 56-57 0.1469566307782202 1107 58-59 0.2132055577953551 1107 60-61 0.0385771662723684 1107 62-63 0.039804692348834436 1107 64-65 -0.03421087427045677 1107 66-67 -0.0019209513422424607 1107 68-69 -0.07273085797521972 1107 70-71 -0.020152804424878212 1107 72-73 -0.012375384895836561 1107 74-75 -0.03832601181655804 1107 76-77 -0.018690361613192152 1108 1 0.25378517059343864 1108 2 0.27789456044921934 1108 3 0.29818502375567846 1108 4 0.21732882622880823 1108 5 0.10735780051430766 1108 6 0.17609836959990588 1108 7 0.2748434427172626 1108 8 0.23338169187397284 1108 9 0.25725903821579976 1108 10-11 0.28850784294263576 1108 12-13 0.27415930835807245 1108 14-15 0.2719539072802277 1108 16-17 0.24821914826215874 1108 18-19 0.20222489875006033 1108 20-21 0.17433080428556025 1108 22-23 0.21437630671891128 1108 24-25 0.3278997979415976 1108 26-27 0.3037002296583111 1108 28-29 0.4569650401991794 1108 30-31 0.543546010864489 1108 32-33 0.5144411174239352 1108 34-35 0.5076933507410288 1108 36-37 0.48588242237278934 1108 38-39 0.5478684248349452 1108 40-41 0.46560501242360175 1108 42-43 0.4677691046237342 1108 44-45 0.3940123025960034 1108 46-47 0.41606084151178635 1108 48-49 0.38499801469732375 1108 50-51 0.30991101501276574 1108 52-53 0.29391305502779375 1108 54-55 0.3595182442191174 1108 56-57 0.3771707834784124 1108 58-59 0.4385399187278125 1108 60-61 0.3282271044599341 1108 62-63 0.32554701534879626 1108 64-65 0.3488195259715745 1108 66-67 0.44496534507576513 1108 68-69 0.32933555518222235 1108 70-71 0.43890493412677856 1108 72-73 0.36420839573000663 1108 74-75 0.47691518708385416 1108 76-77 0.6263439090783258 1109 1 -0.21346259451176763 1109 2 -0.2563845731389378 1109 3 -0.18053898414705571 1109 4 -0.07803450860047434 1109 5 -0.1916641589712924 1109 6 -0.312957996690713 1109 7 -0.27626510636788737 1109 8 -0.15071844379303911 1109 9 -0.19849056071537063 1109 10-11 -0.2204381842836156 1109 12-13 -0.17271132308675874 1109 14-15 -0.09670185444103652 1109 16-17 -0.13260279781149364 1109 18-19 -0.16364143571297518 1109 20-21 -0.10380978387993878 1109 22-23 -0.3014072347575407 1109 24-25 -0.42300357413164136 1109 26-27 -0.4606950462409358 1109 28-29 -0.4553208199967429 1109 30-31 -0.28853511078703775 1109 32-33 -0.3259083411976249 1109 34-35 -0.313827151484837 1109 36-37 -0.16042967881519843 1109 38-39 -0.24561981649758025 1109 40-41 -0.23219091447096218 1109 42-43 -0.24025988065923087 1109 44-45 -0.23876662723370856 1109 46-47 -0.31723741441913234 1109 48-49 -0.30380287977643405 1109 50-51 -0.37572646519171116 1109 52-53 -0.2572567505505461 1109 54-55 -0.20454121713642337 1109 56-57 -0.2930249020010933 1109 58-59 -0.282836534018287 1109 60-61 -0.3073728480824016 1109 62-63 -0.2925600171545426 1109 64-65 -0.3359203854063999 1109 66-67 -0.34164991772103903 1109 68-69 -0.24329659119371527 1109 70-71 -0.2081841573169143 1109 72-73 -0.3327318746754955 1109 74-75 -0.21032398921619944 1109 76-77 -0.27947777907325033 1110 1 -0.22460152235696285 1110 2 -0.18501018179097173 1110 3 -0.34127849717391 1110 4 -0.252201390975614 1110 5 -0.45584393234217657 1110 6 -0.32152258882140927 1110 7 -0.40962123396942474 1110 8 -0.4369236617487431 1110 9 -0.41236389927721717 1110 10-11 -0.4221210966956761 1110 12-13 -0.4119883584410502 1110 14-15 -0.4035465095714841 1110 16-17 -0.4077827373101002 1110 18-19 -0.35727970999369774 1110 20-21 -0.42879584331760157 1110 22-23 -0.42026549948270286 1110 24-25 -0.3728561840753457 1110 26-27 -0.40970285166803677 1110 28-29 -0.3048132933808887 1110 30-31 -0.23731465157911913 1110 32-33 -0.329716400581205 1110 34-35 -0.2452830484392905 1110 36-37 -0.20506845405469676 1110 38-39 -0.2239195443829587 1110 40-41 -0.33211899018399293 1110 42-43 -0.26530781945211146 1110 44-45 -0.2740954892845977 1110 46-47 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0.17363971350003382 2313 66-67 0.06280215605591621 2313 68-69 -0.0019154161323022834 2313 70-71 -0.011913832896787824 2313 72-73 -0.046772843244720974 2313 74-75 -0.06513932376896392 2313 76-77 0.1252720549786268 2314 1 -0.10345451395881611 2314 2 0.049459323023668844 2314 3 -0.028675835586589926 2314 4 0.06845777186335056 2314 5 -0.18730679171244446 2314 6 0.013354498333299603 2314 7 -0.02333007200036974 2314 8 0.024509216406435996 2314 9 0.060308594075259236 2314 10-11 -0.06726183147733877 2314 12-13 0.0046634489327459505 2314 14-15 0.021075407816375957 2314 16-17 0.0037208434339177643 2314 18-19 -0.09877051865714748 2314 20-21 -0.11873089553707672 2314 22-23 0.05595018649367489 2314 24-25 0.11323102546003838 2314 26-27 0.14370743667596741 2314 28-29 0.1312424467063522 2314 30-31 0.004244233379544937 2314 32-33 -0.07892413533844689 2314 34-35 -0.009272208757927558 2314 36-37 -0.5396224379808601 2314 38-39 -0.14827134516799134 2314 40-41 -0.06180449382647879 2314 42-43 -0.05604981190589342 2314 44-45 7.156133947248122E-4 2314 46-47 0.052530473298709524 2314 48-49 0.11020878074517526 2314 50-51 0.1664191549747116 2314 52-53 0.1706169933261279 2314 54-55 0.10532991497376543 2314 56-57 0.1794970998895522 2314 58-59 0.26249051789766753 2314 60-61 0.24019259936334336 2314 62-63 0.24660687142991478 2314 64-65 0.25031892707855974 2314 66-67 0.34040983627508226 2314 68-69 0.43980686276223224 2314 70-71 0.48166857163132093 2314 72-73 0.3777102496222575 2314 74-75 0.3531900263793588 2314 76-77 0.3555937425140847 2315 1 -0.5542156553183801 2315 2 -0.5953425853242109 2315 3 -0.3959042526364911 2315 4 -0.4278173835372101 2315 5 -0.5484171540994964 2315 6 -0.40125294679444323 2315 7 -0.6141191419192182 2315 8 -0.5687228249286633 2315 9 -0.4225335877941916 2315 10-11 -0.5932333700569004 2315 12-13 -0.5840594881481707 2315 14-15 -0.45140215810705087 2315 16-17 -0.4368099339628202 2315 18-19 -0.48018765978449807 2315 20-21 -0.3677195997099787 2315 22-23 -0.4544647091526244 2315 24-25 -0.45557933609765655 2315 26-27 -0.24753990420173722 2315 28-29 -0.30548158719771834 2315 30-31 -0.42960893330855043 2315 32-33 -0.3318375070433106 2315 34-35 -0.4704399522049769 2315 36-37 -0.5448813317859802 2315 38-39 -0.5235476243457668 2315 40-41 -0.535367479258845 2315 42-43 -0.608026322473961 2315 44-45 -0.5697138063464351 2315 46-47 -0.4178690377960024 2315 48-49 -0.2956015310511688 2315 50-51 -0.2849743702439227 2315 52-53 -0.3484690772271861 2315 54-55 -0.5257194586552103 2315 56-57 -0.5387999940474408 2315 58-59 -0.49261624093808365 2315 60-61 -0.4477073056754044 2315 62-63 -0.41679723014959436 2315 64-65 -0.3694042961301349 2315 66-67 -0.3929343650759378 2315 68-69 -0.4529514608607279 2315 70-71 -0.22464070139677972 2315 72-73 -0.009189452224301675 2315 74-75 -0.10429200769887359 2315 76-77 -0.28322410398043374 2316 1 -1.3045415954293418 2316 2 -1.0833218222177479 2316 3 -1.3181626175582508 2316 4 -2.0473311622127284 2316 5 -1.4027465175653546 2316 6 -1.3188602709376198 2316 7 -1.2622963038137343 2316 8 -1.0499560196663964 2316 9 -1.036054855878291 2316 10-11 -1.10684104178209 2316 12-13 -1.651189050981813 2316 14-15 -2.637498057950971 2316 16-17 -1.8326145207337206 2316 18-19 -1.4634155161872187 2316 20-21 -1.2761484634447129 2316 22-23 -1.636128938373119 2316 24-25 -1.835246936207838 2316 26-27 -1.7017113801792334 2316 28-29 -2.0560729684755046 2316 30-31 -1.9898708002830077 2316 32-33 -2.0526643915280403 2316 34-35 -1.958621016199423 2316 36-37 -2.066961806858451 2316 38-39 -2.1323604678289314 2316 40-41 -1.8678131121860062 2316 42-43 -1.945982113977216 2316 44-45 -2.2029108394388643 2316 46-47 -2.1724907308482955 2316 48-49 -2.0726009473757223 2316 50-51 -2.0704546236508854 2316 52-53 -2.1284801605025834 2316 54-55 -2.124658185849242 2316 56-57 -2.020336995136091 2316 58-59 -2.075400438417134 2316 60-61 -1.883359382641867 2316 62-63 -1.8608386579902572 2316 64-65 -1.8806964486464999 2316 66-67 -1.740921504541582 2316 68-69 -1.8437177480290465 2316 70-71 -1.8436788501618828 2316 72-73 -1.890052767238533 2316 74-75 -2.069019647682609 2316 76-77 -2.2463169608422255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20933.0 3 1964.0 4 2986.0 5 3919.0 6 4539.0 7 4970.0 8 6469.0 9 6734.0 10 9746.0 11 12012.0 12 8196.0 13 5591.0 14 5097.0 15 5058.0 16 5010.0 17 5236.0 18 5435.0 19 5810.0 20 6307.0 21 6608.0 22 7397.0 23 8532.0 24 9791.0 25 11492.0 26 13662.0 27 16523.0 28 20012.0 29 24682.0 30 31311.0 31 40819.0 32 54707.0 33 76767.0 34 115867.0 35 173027.0 36 275675.0 37 426720.0 38 446503.0 39 135352.0 40 1215.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.669694090146354 27.096857445434686 29.74253221808128 21.490916246337672 2 21.30975707314999 27.433483490833076 29.71447331404079 21.542286121976144 3 21.517528427885285 27.51356498883058 29.170931220290274 21.79797536299386 4 21.11356645168564 27.554986800003363 29.589551556135955 21.74189519217504 5 21.328190360535512 27.66169114869395 29.580515774046745 21.429602716723792 6 21.645077297490268 27.448396644459233 29.458635731188863 21.447890326861632 7 21.462219952066306 27.249518759360893 29.62390296171288 21.66435832685993 8 21.27368466335996 27.646657136513753 29.321191700954287 21.758466499172005 9 21.334318318247043 27.310387931759138 29.53550445369434 21.819789296299483 10-11 21.65636938005876 27.510308206536482 29.294764640100997 21.538557773303765 12-13 21.659466986300828 27.276701122279846 29.25310238974006 21.810729501679265 14-15 21.332213295928433 27.62295545997222 29.390994493836125 21.65383675026322 16-17 21.616262498037766 27.41913434285877 29.34641241886859 21.618190740234873 18-19 21.49314113123435 27.374880374317446 29.20201665847118 21.92996183597703 20-21 21.48542425594668 27.623912057267102 29.089607790541432 21.80105589624478 22-23 21.548054467878064 27.660362450016144 28.70723589709865 22.084347185007143 24-25 21.57639599393344 27.808566075224768 28.617770553640455 21.99726737720134 26-27 21.685562374129567 27.843194553843247 28.458349588635777 22.01289348339141 28-29 21.721638426165928 27.85788750127624 28.476268807738393 21.944205264819434 30-31 21.579627987488006 27.774265966128226 28.55612955890355 22.08997648748022 32-33 21.574520894725392 28.053270177797994 28.360461206811305 22.01174772066531 34-35 21.57788367048004 27.77863892854715 28.58477493680431 22.058702464168505 36-37 21.74116405742241 27.837764808811578 28.6102168062079 21.81085432755811 38-39 21.552768265538695 27.765255492931768 28.749015090774822 21.93296115075471 40-41 21.499437938218446 27.730326045277927 28.669180596247795 22.10105542025583 42-43 21.442799612044038 27.996120440398748 28.374557980651154 22.18652196690606 44-45 21.5018110343293 27.841716835820158 28.592906679193504 22.06356545065704 46-47 21.440048458177007 27.732268572029 28.653147000105072 22.174535969688915 48-49 21.531879545148318 27.83490550294388 28.467465298337263 22.16574965357054 50-51 21.377711500701853 28.038763682525115 28.39469663608124 22.188828180691793 52-53 21.338760459916088 27.90526711685013 28.444023422596064 22.311949000637714 54-55 21.334030260450138 27.891532558059605 28.46291224380449 22.311524937685764 56-57 21.327985565675263 28.04922761380468 28.37888520157842 22.24390161894164 58-59 21.456249207307923 28.004992136741823 28.341180109282107 22.197578546668147 60-61 21.41220584195813 27.801357699096357 28.312948726919128 22.473487732026385 62-63 21.33216859900312 27.822208068266026 28.416682470360964 22.428940862369892 64-65 21.392053602333977 27.727439054541858 28.445136725510554 22.43537061761361 66-67 21.378559407249025 27.79677293101244 28.441187865595573 22.383479796142954 68-69 21.421102202084807 27.693175408911447 28.439426904567096 22.44629548443665 70-71 21.303104292269246 27.54218465879299 28.522651499712108 22.63205954922566 72-73 21.379835945015532 27.464801100461106 28.478166280741757 22.6771966737816 74-75 21.25290118481642 27.390499599981595 28.4619403638609 22.894658851341088 76-77 21.13669736753571 27.26112988961144 28.787680138371602 22.814492604481245 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 7824.0 1 4697.0 2 1430.5 3 1346.5 4 1402.0 5 1427.5 6 1529.5 7 1680.0 8 1784.5 9 1815.0 10 1887.5 11 2016.5 12 2150.5 13 2228.0 14 2359.5 15 2670.0 16 3092.5 17 3336.0 18 3587.0 19 4341.5 20 5463.5 21 7199.5 22 8317.0 23 9717.0 24 12777.0 25 17049.0 26 19661.0 27 22870.5 28 29436.5 29 35865.0 30 38937.0 31 43770.0 32 52971.0 33 61231.5 34 68603.5 35 72083.0 36 76258.0 37 83015.0 38 86885.5 39 88174.0 40 89817.5 41 92483.5 42 95776.0 43 98046.0 44 97269.5 45 96060.0 46 94997.0 47 93982.5 48 93598.0 49 92268.5 50 88537.0 51 82710.5 52 79286.0 53 76820.5 54 70415.5 55 60832.5 56 50875.5 57 46562.0 58 43422.5 59 35783.5 60 28606.0 61 25928.0 62 22488.5 63 16039.5 64 11224.0 65 9418.0 66 8104.5 67 5942.0 68 4377.5 69 3067.5 70 2473.0 71 2089.5 72 1476.0 73 1112.0 74 978.0 75 912.0 76 689.5 77 413.5 78 294.0 79 235.5 80 173.0 81 138.0 82 87.5 83 68.0 84 62.5 85 55.5 86 41.0 87 28.0 88 29.0 89 32.5 90 26.5 91 18.0 92 16.5 93 18.5 94 18.5 95 13.5 96 12.0 97 16.0 98 21.0 99 40.5 100 59.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0025708542256438756 2 0.006476575068448993 3 0.009294626815789396 4 0.01606783891027422 5 0.011766602032754661 6 0.011272206989361608 7 0.014683532788773674 8 0.015870080892917 9 0.015227367336506032 10-11 0.01866341288808775 12-13 0.01789710057082852 14-15 0.006056339281564899 16-17 0.005265307212136014 18-19 0.010851971202477512 20-21 0.010950850211156122 22-23 0.013398105675951736 24-25 0.021802821413633634 26-27 0.012705952615201462 28-29 0.006699052837975868 30-31 0.006649613333636562 32-33 0.007613683668253016 34-35 0.009220467559280437 36-37 0.007588963916083363 38-39 0.013818341462835832 40-41 0.01035757615908446 42-43 0.013818341462835832 44-45 0.017254387014417547 46-47 0.0154004056016936 48-49 0.012681232863031806 50-51 0.02588158052162632 52-53 0.030133377894806575 54-55 0.022915210261268002 56-57 0.013472264932460693 58-59 0.014510494523586104 60-61 0.01757574379262303 62-63 0.023112968278625225 64-65 0.01849037462290018 66-67 0.018515094375069832 68-69 0.028056918712555754 70-71 0.053691301712485553 72-73 0.06622421606249945 74-75 0.07499972808272613 76-77 0.07168728129199269 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2022674.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.82194679211962 #Duplication Level Percentage of deduplicated Percentage of total 1 75.24195581781538 44.25878321650552 2 13.874047508767223 16.32196968704091 3 4.909889767366974 8.664278239536982 4 2.2087383849806947 5.196891670361866 5 1.1540552704566098 3.3941888856981963 6 0.6706818156501714 2.3670486044769934 7 0.417604419545722 1.719501346267072 8 0.2711973416844202 1.2761884482180201 9 0.1981418025102206 1.0489577908095782 >10 0.9637758473501687 10.181846595109434 >50 0.06505341840036805 2.608281873135826 >100 0.024437552346880272 2.3786483152300826 >500 2.526318751232717E-4 0.09020201320912133 >1k 8.421062504108865E-5 0.13314827649978767 >5k 8.421062504108865E-5 0.3600650379006535 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7269 0.3593757570424102 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2688 0.13289338766405262 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.483185130179159E-4 4.9439504339305296E-5 3 0.0 0.0 0.0 1.9775801735722118E-4 4.9439504339305296E-5 4 0.0 4.9439504339305296E-5 0.0 6.921530607502741E-4 4.9439504339305296E-5 5 0.0 4.9439504339305296E-5 0.0 0.0016315036431970747 4.9439504339305296E-5 6 0.0 9.887900867861059E-5 0.0 0.0031146887733762337 4.9439504339305296E-5 7 0.0 1.483185130179159E-4 0.0 0.006575454077127604 4.9439504339305296E-5 8 0.0 1.483185130179159E-4 0.0 0.010678932937289944 4.9439504339305296E-5 9 0.0 1.483185130179159E-4 0.0 0.015524004362541862 4.9439504339305296E-5 10 0.0 1.483185130179159E-4 0.0 0.021061228848544056 4.9439504339305296E-5 11 0.0 1.483185130179159E-4 0.0 0.027043408873599996 4.9439504339305296E-5 12 0.0 1.483185130179159E-4 0.0 0.03559644312429981 4.9439504339305296E-5 13 0.0 1.483185130179159E-4 0.0 0.04657201308762559 4.9439504339305296E-5 14 0.0 1.483185130179159E-4 0.0 0.05952516322452358 4.9439504339305296E-5 15 0.0 1.483185130179159E-4 0.0 0.07588963916083363 4.9439504339305296E-5 16 0.0 1.483185130179159E-4 0.0 0.09156196203639341 4.9439504339305296E-5 17 0.0 1.483185130179159E-4 0.0 0.10664101085988152 4.9439504339305296E-5 18 0.0 1.483185130179159E-4 0.0 0.12251109175279852 4.9439504339305296E-5 19 0.0 1.9775801735722118E-4 0.0 0.13843061215005484 4.9439504339305296E-5 20 0.0 1.9775801735722118E-4 0.0 0.15795921636408042 4.9439504339305296E-5 21 0.0 1.9775801735722118E-4 0.0 0.18149242042958974 4.9439504339305296E-5 22 0.0 1.9775801735722118E-4 0.0 0.20576721706018863 4.9439504339305296E-5 23 0.0 1.9775801735722118E-4 0.0 0.23098136427323435 4.9439504339305296E-5 24 0.0 1.9775801735722118E-4 0.0 0.25807421265117364 4.9439504339305296E-5 25 0.0 1.9775801735722118E-4 0.0 0.28665024615929213 4.9439504339305296E-5 26 0.0 1.9775801735722118E-4 0.0 0.3165117067802325 4.9439504339305296E-5 27 0.0 1.9775801735722118E-4 0.0 0.3453843773143868 4.9439504339305296E-5 28 0.0 1.9775801735722118E-4 0.0 0.37643238603947055 4.9439504339305296E-5 29 0.0 1.9775801735722118E-4 0.0 0.40827142683398315 4.9439504339305296E-5 30 4.9439504339305296E-5 3.4607653037513705E-4 0.0 0.4416925317673535 4.9439504339305296E-5 31 4.9439504339305296E-5 3.4607653037513705E-4 0.0 0.48040366366502957 4.9439504339305296E-5 32 4.9439504339305296E-5 3.4607653037513705E-4 0.0 0.5182743239889375 4.9439504339305296E-5 33 4.9439504339305296E-5 3.4607653037513705E-4 0.0 0.5617810878075261 4.9439504339305296E-5 34 4.9439504339305296E-5 3.9551603471444237E-4 0.0 0.609539648999295 4.9439504339305296E-5 35 4.9439504339305296E-5 3.9551603471444237E-4 0.0 0.6580398027561535 4.9439504339305296E-5 36 4.9439504339305296E-5 4.449555390537477E-4 0.0 0.7095557662777096 4.9439504339305296E-5 37 4.9439504339305296E-5 4.449555390537477E-4 0.0 0.7610717297992657 4.9439504339305296E-5 38 4.9439504339305296E-5 4.449555390537477E-4 0.0 0.8154551845725015 4.9439504339305296E-5 39 4.9439504339305296E-5 4.449555390537477E-4 0.0 0.8738432391972211 4.9439504339305296E-5 40 4.9439504339305296E-5 4.449555390537477E-4 0.0 0.9335167209347626 4.9439504339305296E-5 41 4.9439504339305296E-5 4.449555390537477E-4 0.0 0.9991723826973601 4.9439504339305296E-5 42 4.9439504339305296E-5 4.449555390537477E-4 0.0 1.068832644311441 4.9439504339305296E-5 43 4.9439504339305296E-5 4.449555390537477E-4 0.0 1.1488752018367765 4.9439504339305296E-5 44 4.9439504339305296E-5 4.449555390537477E-4 0.0 1.2316863716051127 4.9439504339305296E-5 45 4.9439504339305296E-5 4.449555390537477E-4 0.0 1.3185515807292723 4.9439504339305296E-5 46 4.9439504339305296E-5 4.449555390537477E-4 0.0 1.4102618612786835 4.9439504339305296E-5 47 4.9439504339305296E-5 4.449555390537477E-4 0.0 1.5006372752109336 9.887900867861059E-5 48 4.9439504339305296E-5 4.449555390537477E-4 0.0 1.5952150470120248 9.887900867861059E-5 49 4.9439504339305296E-5 4.449555390537477E-4 0.0 1.6954783618121358 9.887900867861059E-5 50 4.9439504339305296E-5 4.943950433930529E-4 0.0 1.7995485184463735 9.887900867861059E-5 51 4.9439504339305296E-5 4.943950433930529E-4 0.0 1.9120728303226324 9.887900867861059E-5 52 4.9439504339305296E-5 4.943950433930529E-4 0.0 2.0278601494852855 9.887900867861059E-5 53 4.9439504339305296E-5 4.943950433930529E-4 0.0 2.1503218017337447 9.887900867861059E-5 54 4.9439504339305296E-5 4.943950433930529E-4 0.0 2.2825724758413863 9.887900867861059E-5 55 4.9439504339305296E-5 4.943950433930529E-4 0.0 2.4262931149557465 9.887900867861059E-5 56 1.483185130179159E-4 4.943950433930529E-4 0.0 2.5719913342436795 9.887900867861059E-5 57 1.483185130179159E-4 4.943950433930529E-4 0.0 2.72495716066949 9.887900867861059E-5 58 1.483185130179159E-4 5.438345477323583E-4 0.0 2.882570300503195 9.887900867861059E-5 59 1.483185130179159E-4 5.438345477323583E-4 0.0 3.0419138229887763 9.887900867861059E-5 60 1.483185130179159E-4 5.438345477323583E-4 0.0 3.207338404508092 9.887900867861059E-5 61 1.483185130179159E-4 5.438345477323583E-4 0.0 3.3813654597824465 9.887900867861059E-5 62 1.483185130179159E-4 5.438345477323583E-4 0.0 3.5619185296295894 9.887900867861059E-5 63 1.483185130179159E-4 5.438345477323583E-4 0.0 3.7536449274574153 9.887900867861059E-5 64 1.483185130179159E-4 5.438345477323583E-4 0.0 3.9562480162398885 9.887900867861059E-5 65 1.483185130179159E-4 5.438345477323583E-4 0.0 4.168640126881544 9.887900867861059E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGCT 175 1.6916601E-10 24.340466 37 GACGTAT 185 8.480129E-9 21.107079 39 CGATCGT 205 1.2223609E-9 20.778961 1 CTCGCGC 215 2.2118911E-9 19.811516 36 TACGTAG 185 1.960343E-7 19.187778 2 CGCGGAA 205 2.755769E-8 19.050207 59 CGCCCAA 115 9.16442E-4 18.522669 58 GTCGGGT 270 1.0004442E-10 18.412428 66 CGCGCTT 235 6.672053E-9 18.12588 38 CAATTCG 255 9.349606E-10 18.097605 54 TCGCGGA 220 6.187656E-8 17.75089 58 ATTCGCG 240 8.651114E-9 17.749573 56 ATACGTA 200 4.4464832E-7 17.748695 1 CGGCGCA 100 0.008937684 17.748257 43 ATTCCGC 145 2.1903451E-4 17.13752 54 CTCCGCG 290 2.8194336E-10 17.133705 27 CGCTCCG 260 2.329034E-8 16.38179 25 TATTCCG 135 0.0026812144 15.778178 53 CGCGCCC 295 6.49743E-9 15.639825 30 ACTCGAG 275 4.64388E-8 15.488238 14 >>END_MODULE