FastQCFastQC Report
Thu 26 May 2016
SRR936016_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936016_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022674
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75960.375542474961363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57360.2835849968902552No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40240.19894456546136452No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA9550.046.74041100-101
GGTATCA64650.042.6222461
CGAGTAC7700.039.033306100-101
CGGTATC6350.038.896362100-101
CGTACAT10450.037.304363100-101
TACGCAG7600.036.022915100-101
CCGTACA4900.032.794598100-101
TCGAGTA3450.031.051891100-101
GTATCAA124500.031.0241051
GCGTATC2600.030.902601100-101
CCGTATC4750.030.697788100-101
GACGCGT703.0922038E-629.75585496-97
CCTGTCG5250.029.47246696-97
TCGTATC5250.028.907833100-101
TCGTACA5050.028.28489100-101
CCGAGTA3950.027.874638100-101
GGGTATC11050.027.738283100-101
AGTATCA25100.027.3869100-101
ACCGTAC2100.026.9229698-99
CCCGTAC2750.025.96970798-99
Produced by FastQC (version 0.11.5)