FastQCFastQC Report
Thu 26 May 2016
SRR936015_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936015_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000759
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46350.1544609213868891No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG1102.6195865E-522.59350452
ACTACGG1350.002670796415.78774967
TCGAACT9200.014.66423824
ACGCGAA1707.3454896E-414.61810754
AACGCGA2001.5551646E-414.2009253
CCGGTTA2252.662735E-514.19642334
GTAACGT2001.5602383E-414.19547752
CGAACTC10150.013.98918925
TAAGGCG10450.013.92797217
GGTAACG2051.9230203E-413.8501691
ATACGTA1550.006728204413.7384751
GCCTCGA10150.012.94215721
ATAACGC1950.0020668312.74547851
CTCGAAC10700.012.60892523
CGTCGTG3103.0184347E-612.5923424
CGCCGTA2305.048342E-412.3556367
TTGGACG3204.2829197E-612.20452645
CCGGGGT7300.012.15933648
CCGTATC2651.2244254E-412.06482769
CGTGCGC5409.094947E-1111.83508746
Produced by FastQC (version 0.11.5)