##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936015_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3000759 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.52602924793361 40.0 37.0 41.0 32.0 41.0 2 37.402666125470255 40.0 37.0 41.0 32.0 41.0 3 37.32201019808655 40.0 37.0 41.0 32.0 41.0 4 37.21530552770149 40.0 37.0 41.0 31.0 41.0 5 37.14410754079218 40.0 37.0 41.0 31.0 41.0 6 37.05345047702931 40.0 37.0 41.0 31.0 41.0 7 36.89772254286332 39.0 36.0 41.0 31.0 41.0 8 36.72211630457494 39.0 36.0 41.0 30.0 41.0 9 36.64745186134574 39.0 36.0 41.0 30.0 41.0 10-11 36.86259609652092 39.0 36.0 41.0 31.0 41.0 12-13 37.07372151512334 40.0 37.0 41.0 31.0 41.0 14-15 37.03279287007054 40.0 37.0 41.0 31.0 41.0 16-17 36.89189068499003 40.0 37.0 41.0 31.0 41.0 18-19 36.76077802316014 40.0 36.0 41.0 30.0 41.0 20-21 36.5876204986805 40.0 36.0 41.0 30.0 41.0 22-23 36.3654252140875 40.0 36.0 41.0 30.0 41.0 24-25 36.16446805624844 39.0 35.5 41.0 29.5 41.0 26-27 35.23415625846661 38.0 34.0 40.0 28.0 40.5 28-29 35.28035057130546 38.5 34.5 40.0 28.0 40.5 30-31 35.663013924143854 39.0 35.0 40.5 28.0 41.0 32-33 35.51244885044084 39.0 35.0 41.0 28.0 41.0 34-35 35.24974314831681 38.5 35.0 40.0 27.5 41.0 36-37 34.96434102172151 38.0 34.0 40.0 26.5 41.0 38-39 34.685735342291736 38.0 34.0 40.0 26.0 41.0 40-41 34.3184067764189 37.0 34.0 40.0 26.0 41.0 42-43 33.95365155948878 36.5 34.0 39.5 26.0 41.0 44-45 33.581451226173115 36.0 33.0 39.0 25.0 41.0 46-47 33.19315746449482 35.5 33.0 39.0 24.5 40.5 48-49 32.737578725915675 35.0 32.5 38.0 23.0 40.0 50-51 32.30627167993164 35.0 32.0 37.0 22.0 39.5 52-53 31.870355466733585 35.0 32.0 37.0 20.5 39.0 54-55 31.415568694453636 35.0 31.5 36.0 19.5 39.0 56-57 30.98023999928018 35.0 31.0 36.0 18.0 37.5 58-59 30.564010638641758 34.0 31.0 35.5 14.5 37.0 60-61 30.217487142419635 34.0 31.0 35.0 9.5 37.0 62-63 29.892103964363685 34.0 30.0 35.0 7.0 36.0 64-65 29.52967882459071 34.0 30.0 35.0 2.0 36.0 66-67 29.1649892577178 34.0 29.5 35.0 2.0 35.5 68-69 28.825392175779527 34.0 29.0 35.0 2.0 35.0 70-71 28.430471090814024 34.0 29.0 35.0 2.0 35.0 72-73 27.981011470764564 34.0 29.0 35.0 2.0 35.0 74-75 27.416402650129516 34.0 27.5 35.0 2.0 35.0 76-77 25.67125217320018 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7607012126955937 1101 2 0.7272935989481368 1101 3 0.7803640265289218 1101 4 0.7423527379931798 1101 5 0.8601927090552763 1101 6 0.7784161797958831 1101 7 0.8161182867776731 1101 8 0.9452433301827767 1101 9 0.9296334727514619 1101 10-11 0.8045118150923329 1101 12-13 0.9180684927211971 1101 14-15 0.883852471896617 1101 16-17 0.8517034027954722 1101 18-19 0.8965696495141842 1101 20-21 0.8212348796109623 1101 22-23 0.8108567057792158 1101 24-25 0.9216848303437644 1101 26-27 0.8776707615954038 1101 28-29 0.8511096078173139 1101 30-31 0.8844546022160955 1101 32-33 1.0409453308606444 1101 34-35 0.9866136531182974 1101 36-37 1.0241729321941762 1101 38-39 1.0016960353949713 1101 40-41 0.9534541383320132 1101 42-43 0.7977951112905544 1101 44-45 0.7619918646954105 1101 46-47 0.7752592206368689 1101 48-49 0.9153396489572216 1101 50-51 0.8360421979096628 1101 52-53 0.809890897626456 1101 54-55 0.7620239631500993 1101 56-57 0.7695641713101473 1101 58-59 0.7813872699911251 1101 60-61 0.6070132342283401 1101 62-63 0.6453487651021916 1101 64-65 0.6234062810687853 1101 66-67 0.718944928913114 1101 68-69 0.6838356606212699 1101 70-71 0.7675720598068665 1101 72-73 0.7603101255820768 1101 74-75 0.8285336662506246 1101 76-77 0.8909903608421779 1102 1 0.5730102599607534 1102 2 0.6341896940966265 1102 3 0.5831272131170664 1102 4 0.550657041671677 1102 5 0.6485371301826461 1102 6 0.5618948003997559 1102 7 0.45013356732079757 1102 8 0.585392470227518 1102 9 0.4849365353465842 1102 10-11 0.43407205520397696 1102 12-13 0.5299254825205111 1102 14-15 0.6404205149570856 1102 16-17 0.7333103905870573 1102 18-19 0.65090062279166 1102 20-21 0.7115875206763533 1102 22-23 0.6841743088388696 1102 24-25 0.7332545197393259 1102 26-27 0.5756362764864491 1102 28-29 0.540472120921585 1102 30-31 0.6632490442546981 1102 32-33 0.6454617665153037 1102 34-35 0.6881458670261367 1102 36-37 0.622965032915701 1102 38-39 0.5888522844361574 1102 40-41 0.6870953950159304 1102 42-43 0.672228392093551 1102 44-45 0.6769404844200082 1102 46-47 0.4650518503683827 1102 48-49 0.5167934791934101 1102 50-51 0.6703279654405634 1102 52-53 0.673354788730709 1102 54-55 0.6722569499396727 1102 56-57 0.7098400451804885 1102 58-59 0.6797594368584576 1102 60-61 0.40486917877474937 1102 62-63 0.47557038095692405 1102 64-65 0.4152997950027064 1102 66-67 0.4307905210079035 1102 68-69 0.3765841259466107 1102 70-71 0.2896164764657563 1102 72-73 0.4239232473361234 1102 74-75 0.5856815302054841 1102 76-77 0.7600530782814481 1103 1 0.4568839115704293 1103 2 0.31918134048318336 1103 3 0.3962224966593695 1103 4 0.43690047307475055 1103 5 0.4286643757497046 1103 6 0.4492788422202807 1103 7 0.5532358517798244 1103 8 0.5098299527239334 1103 9 0.5224179765029291 1103 10-11 0.4591271019421015 1103 12-13 0.5756518558232102 1103 14-15 0.5751575802021733 1103 16-17 0.647069483528135 1103 18-19 0.6510671881224681 1103 20-21 0.6199776233023186 1103 22-23 0.6059042770559273 1103 24-25 0.5632572706537076 1103 26-27 0.4317857495244368 1103 28-29 0.48549252620917827 1103 30-31 0.7940574540865271 1103 32-33 0.6776502063226815 1103 34-35 0.7590325542868968 1103 36-37 0.6308183813495774 1103 38-39 0.636961679993334 1103 40-41 0.6039193935004974 1103 42-43 0.6935379432082627 1103 44-45 0.6411757524665589 1103 46-47 0.4160717112666674 1103 48-49 0.46736651048689737 1103 50-51 0.5052108219056279 1103 52-53 0.5597468357224038 1103 54-55 0.6224074639592487 1103 56-57 0.529517090522571 1103 58-59 0.5045112415834758 1103 60-61 0.483547709445066 1103 62-63 0.41265963835178 1103 64-65 0.4354010232720107 1103 66-67 0.4441147646331842 1103 68-69 0.4752556174212046 1103 70-71 0.37444038846050276 1103 72-73 0.3662701238792465 1103 74-75 0.3846989913215282 1103 76-77 0.5734865618649891 1104 1 0.42549787512101744 1104 2 0.3170743180384221 1104 3 0.35800976964297604 1104 4 0.34211585085797225 1104 5 0.39201896680555137 1104 6 0.1366122499312965 1104 7 0.4021956984996393 1104 8 0.48293516824674043 1104 9 0.28300138760652516 1104 10-11 0.24330626390447918 1104 12-13 0.4155991056772237 1104 14-15 0.3640856744873915 1104 16-17 0.39002823198580217 1104 18-19 0.3332988874567846 1104 20-21 0.24155799786958454 1104 22-23 0.2009316626502553 1104 24-25 0.23143738854544438 1104 26-27 0.17490117799428617 1104 28-29 0.21692765400256775 1104 30-31 0.3606564039109017 1104 32-33 0.40178202554180587 1104 34-35 0.4446626301365981 1104 36-37 0.30716362557512866 1104 38-39 0.37738972253529823 1104 40-41 0.3376788757489422 1104 42-43 0.3645280976952705 1104 44-45 0.2899807941548431 1104 46-47 0.10552146453061084 1104 48-49 0.3368925122729962 1104 50-51 0.2511141186913406 1104 52-53 0.19186408354502404 1104 54-55 0.309247523863327 1104 56-57 0.35195564683720093 1104 58-59 0.33725126267234273 1104 60-61 0.12102486394543632 1104 62-63 0.20896236298870363 1104 64-65 0.17310355873271277 1104 66-67 0.09741903473067381 1104 68-69 0.01774847980498251 1104 70-71 -0.03454955325246445 1104 72-73 -0.12865204525995821 1104 74-75 -0.1266108711905929 1104 76-77 -0.07755296987465243 1105 1 -0.07128069930666214 1105 2 0.17299619604917638 1105 3 0.15532080276261695 1105 4 0.08562419660945153 1105 5 0.08179740912827782 1105 6 -0.2040178486257318 1105 7 -0.12609863722700965 1105 8 0.12278025204924603 1105 9 0.11817700305152812 1105 10-11 0.12200541296346756 1105 12-13 0.22024417248278638 1105 14-15 -0.024158583364652486 1105 16-17 0.19860941636982687 1105 18-19 0.2925225268388729 1105 20-21 0.3027209085203566 1105 22-23 0.22447432203235707 1105 24-25 0.32174521833030667 1105 26-27 0.09406125554073697 1105 28-29 0.34601922629553883 1105 30-31 0.3027208614517818 1105 32-33 0.4009738293129459 1105 34-35 0.3901102261209459 1105 36-37 0.4858023387214203 1105 38-39 0.411613530203951 1105 40-41 0.48651635215725975 1105 42-43 0.25960252873942835 1105 44-45 0.21682751833063918 1105 46-47 0.14163639247450988 1105 48-49 0.15231843715491777 1105 50-51 0.3726954297110012 1105 52-53 0.24818970206125712 1105 54-55 0.17091191796017213 1105 56-57 0.344210362857833 1105 58-59 0.39385676278214987 1105 60-61 0.09154810474079156 1105 62-63 0.04886172972635805 1105 64-65 0.2164582798860799 1105 66-67 0.31966747576600696 1105 68-69 0.06007944015750866 1105 70-71 -0.316262209832594 1105 72-73 -0.3586786387444114 1105 74-75 -0.3357008805220829 1105 76-77 -0.2487130280681491 1106 1 0.12929172144423973 1106 2 0.21289374918861625 1106 3 -0.1964438725553137 1106 4 -0.07600174678064775 1106 5 -0.23121238957186563 1106 6 0.0335397939706894 1106 7 0.21256217100337693 1106 8 0.3259313806533939 1106 9 0.1983993681624412 1106 10-11 -0.10565058468103672 1106 12-13 -0.03835285543246414 1106 14-15 0.18710667433293793 1106 16-17 0.2723802488912881 1106 18-19 0.232353939834816 1106 20-21 0.018208743496906266 1106 22-23 0.04651446942420279 1106 24-25 0.19770333029443066 1106 26-27 0.1613532767228989 1106 28-29 0.11458652903336741 1106 30-31 0.19252461682634703 1106 32-33 0.1253359179125937 1106 34-35 0.10699988574276631 1106 36-37 0.11021855182475093 1106 38-39 0.08740234072055131 1106 40-41 0.107822571279236 1106 42-43 0.08855930058083317 1106 44-45 -0.017562379439006293 1106 46-47 0.18501068723222858 1106 48-49 0.12248100551416741 1106 50-51 -0.042896919682164025 1106 52-53 0.02741444432037099 1106 54-55 0.18664509931189954 1106 56-57 -0.022780726083883707 1106 58-59 -0.0383055327895967 1106 60-61 -0.1796034265496651 1106 62-63 -0.2355565292832047 1106 64-65 -0.2378687576849856 1106 66-67 -0.32074831949615046 1106 68-69 -0.4243250712281217 1106 70-71 -0.5007335649802691 1106 72-73 -0.3574187285477848 1106 74-75 -0.28234380818143734 1106 76-77 -0.22970017241603458 1107 1 0.022995298581662382 1107 2 -0.01657248515616061 1107 3 -0.09304471227801514 1107 4 -0.003891482237513344 1107 5 -0.07363758488948946 1107 6 -0.2467081119367336 1107 7 -0.06586991878951665 1107 8 -0.04194952012060327 1107 9 -0.09275038666441304 1107 10-11 0.005204149495014576 1107 12-13 0.04794156732636168 1107 14-15 -0.01024072817938304 1107 16-17 0.08769387745918777 1107 18-19 -0.035257802592461474 1107 20-21 -0.10992244833496301 1107 22-23 -0.13056380682862567 1107 24-25 -0.34541617911328615 1107 26-27 -0.2142610198475623 1107 28-29 -0.1615393983543001 1107 30-31 -5.8741775674775454E-5 1107 32-33 0.08404372254203452 1107 34-35 0.131940537744498 1107 36-37 0.09204028268458586 1107 38-39 0.029112686123497156 1107 40-41 0.07104921993427382 1107 42-43 0.08735544134321316 1107 44-45 0.03751646689688215 1107 46-47 -0.0357331160559653 1107 48-49 0.24499436583285217 1107 50-51 0.08616774303029473 1107 52-53 0.17364550550375668 1107 54-55 0.24340258887151833 1107 56-57 0.20523735827664424 1107 58-59 0.1970266742821032 1107 60-61 0.18102094808367397 1107 62-63 0.2691282591790838 1107 64-65 0.27573114562551027 1107 66-67 0.15348398025415477 1107 68-69 0.14369134459665744 1107 70-71 0.20155430770617855 1107 72-73 0.21626065482129775 1107 74-75 0.26244225826158285 1107 76-77 0.41616808919967596 1108 1 -0.07764440164640973 1108 2 -0.15121085170264337 1108 3 -0.08550663887883303 1108 4 -0.06297766229036483 1108 5 -0.023787222796393337 1108 6 0.033656855720764156 1108 7 0.011561678958109667 1108 8 0.20061345401852293 1108 9 0.01831873277012619 1108 10-11 -0.04058059954959248 1108 12-13 -0.006824009680620691 1108 14-15 0.15697908560130713 1108 16-17 0.1369820942218425 1108 18-19 0.13807447527440075 1108 20-21 0.1159975949732086 1108 22-23 0.08656400626917815 1108 24-25 0.13836915651775428 1108 26-27 -0.009751016506058363 1108 28-29 0.056433639098216304 1108 30-31 0.1204012324553787 1108 32-33 0.16588055520875855 1108 34-35 0.17789455053558356 1108 36-37 0.12384472177225092 1108 38-39 0.1461253706902852 1108 40-41 0.15791690675548153 1108 42-43 0.1228326118954044 1108 44-45 0.08304367239619381 1108 46-47 0.12236469504277636 1108 48-49 0.0020855683496421307 1108 50-51 0.14268031159305394 1108 52-53 0.09296907796104392 1108 54-55 0.0975205861311963 1108 56-57 0.16251467795603602 1108 58-59 0.159968127995036 1108 60-61 0.0898242679877761 1108 62-63 0.17513398496305754 1108 64-65 0.24796350097431485 1108 66-67 0.1806796301758702 1108 68-69 0.18790675606077656 1108 70-71 0.12251616353723804 1108 72-73 0.11329503724938661 1108 74-75 -0.07940922447424725 1108 76-77 -0.0353323755460444 1109 1 0.002051524220071599 1109 2 -0.06691303345298394 1109 3 -0.22396232058362386 1109 4 -0.031903446336563945 1109 5 0.006596329695327086 1109 6 -0.07550224416080198 1109 7 -0.20770227856088042 1109 8 0.040134414700162324 1109 9 -0.026288712686110216 1109 10-11 -0.24144588112944376 1109 12-13 -0.04413935915546574 1109 14-15 0.20499180127095684 1109 16-17 0.2581026436290088 1109 18-19 0.09202399003125805 1109 20-21 0.18749319372897588 1109 22-23 0.2883483648241665 1109 24-25 0.2160484058116907 1109 26-27 0.07374128077422881 1109 28-29 0.18982387696930658 1109 30-31 0.24881865001610493 1109 32-33 0.2097188132791601 1109 34-35 0.4234548035474788 1109 36-37 0.42083966326975997 1109 38-39 0.3124783031900833 1109 40-41 0.3446112230984397 1109 42-43 0.37375190578745787 1109 44-45 0.3075645029276828 1109 46-47 0.20799698616263385 1109 48-49 0.17815648716630506 1109 50-51 0.36288568852958036 1109 52-53 0.41778947075419737 1109 54-55 0.4862152018194976 1109 56-57 0.4587962881736871 1109 58-59 0.4395359644223298 1109 60-61 0.3484386894920526 1109 62-63 0.3047940110798528 1109 64-65 0.24819115527816393 1109 66-67 0.3455666381540752 1109 68-69 0.33075272769671926 1109 70-71 0.3171187283628605 1109 72-73 0.2895756062658421 1109 74-75 0.21698171186931603 1109 76-77 0.4404952709487979 1110 1 -0.5265256743133406 1110 2 -0.6049255120399337 1110 3 -0.5078953277671516 1110 4 -0.4087513578732995 1110 5 -0.46535500338245583 1110 6 -0.6166477966338917 1110 7 -0.6423163887631347 1110 8 -0.563316796017304 1110 9 -0.5199713468330884 1110 10-11 -0.5933214590924578 1110 12-13 -0.5688191107964968 1110 14-15 -0.5776930765094903 1110 16-17 -0.6421039612294521 1110 18-19 -0.5110988510670822 1110 20-21 -0.5964160993748848 1110 22-23 -0.830670435351692 1110 24-25 -0.7182708485050284 1110 26-27 -0.8014085835465821 1110 28-29 -0.6424633297719922 1110 30-31 -0.5745340877148379 1110 32-33 -0.5582415159892165 1110 34-35 -0.3557576399556268 1110 36-37 -0.4649225224103972 1110 38-39 -0.42755192955440435 1110 40-41 -0.41873839606859775 1110 42-43 -0.4965641303445665 1110 44-45 -0.5844250650429288 1110 46-47 -0.6715230365335856 1110 48-49 -0.5516045203897093 1110 50-51 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-0.4712863841523145 2314 5 -0.4724176174852275 2314 6 -0.5978055190000688 2314 7 -0.6543458368082042 2314 8 -0.6413097070781149 2314 9 -0.5693165965178721 2314 10-11 -0.5880725924124874 2314 12-13 -0.6852941764396121 2314 14-15 -0.6517538811679771 2314 16-17 -1.0675710770019933 2314 18-19 -0.633735808057871 2314 20-21 -0.5855470305041237 2314 22-23 -0.45105047231412954 2314 24-25 -0.44849284082154384 2314 26-27 -0.37898555833515246 2314 28-29 -0.5456058153840715 2314 30-31 -0.5721135978111676 2314 32-33 -0.6537962426422297 2314 34-35 -0.6877343710144501 2314 36-37 -0.8558127080457538 2314 38-39 -0.7025293937667243 2314 40-41 -0.5123745812282792 2314 42-43 -0.4672820345279618 2314 44-45 -0.5559687161270688 2314 46-47 -0.45243928703615666 2314 48-49 -0.5066913005113989 2314 50-51 -0.40328002663458307 2314 52-53 -0.36263104000364166 2314 54-55 -0.467415161043057 2314 56-57 -0.24425290706788516 2314 58-59 -0.33104781447811504 2314 60-61 -0.17737801974829992 2314 62-63 -0.29625465979995624 2314 64-65 -0.2815315752778069 2314 66-67 -0.4079184722883049 2314 68-69 -0.16974778508275534 2314 70-71 -0.018276901534605372 2314 72-73 0.015332929957097008 2314 74-75 0.12031172494677378 2314 76-77 -0.026476150282167765 2315 1 -0.8349830070637623 2315 2 -0.7556944255291924 2315 3 -0.8626611731491138 2315 4 -0.7390383781399805 2315 5 -0.6575648343372862 2315 6 -0.658567211325412 2315 7 -0.7824275978940705 2315 8 -0.6702506794760623 2315 9 -0.7193833579917381 2315 10-11 -0.8624634624962582 2315 12-13 -0.7771874124859153 2315 14-15 -1.0759923132559948 2315 16-17 -0.9010389454595398 2315 18-19 -0.8277521159079768 2315 20-21 -0.812203710362823 2315 22-23 -0.9227017813113676 2315 24-25 -1.1259231609054865 2315 26-27 -0.9262548854233614 2315 28-29 -0.8151697634290471 2315 30-31 -0.9121701552808616 2315 32-33 -0.8554294421152235 2315 34-35 -0.7497935997865781 2315 36-37 -1.0392545129588626 2315 38-39 -0.8988032241967971 2315 40-41 -0.8681271492043692 2315 42-43 -1.096002099408473 2315 44-45 -0.9698929644173333 2315 46-47 -0.8782432714688895 2315 48-49 -0.7299709484258088 2315 50-51 -0.7575949349820021 2315 52-53 -0.7875492118610872 2315 54-55 -0.7547667928402433 2315 56-57 -1.0193688539927273 2315 58-59 -0.8641480941762119 2315 60-61 -0.906599828946284 2315 62-63 -0.8544693576146649 2315 64-65 -0.8967024454290602 2315 66-67 -0.7859160962040086 2315 68-69 -0.8413407990406725 2315 70-71 -1.0074031739247928 2315 72-73 -2.3833674459821204 2315 74-75 -2.9524464201217384 2315 76-77 -2.7931639355894156 2316 1 -2.812624547550925 2316 2 -2.6941695736529567 2316 3 -2.2003833587975663 2316 4 -2.2371876539793547 2316 5 -2.842599670852543 2316 6 -2.554554428793047 2316 7 -2.7616565314029557 2316 8 -2.5604983753285993 2316 9 -2.1693828093851977 2316 10-11 -2.177528609522817 2316 12-13 -2.216489722119441 2316 14-15 -2.365684133532497 2316 16-17 -3.6293305846958788 2316 18-19 -3.2383287013808726 2316 20-21 -2.7490305701389914 2316 22-23 -2.782077604221179 2316 24-25 -2.7748610586452287 2316 26-27 -2.6654375988313035 2316 28-29 -2.8690913401242426 2316 30-31 -2.86275510700348 2316 32-33 -3.112326112073532 2316 34-35 -3.157488217956427 2316 36-37 -3.1030728144730126 2316 38-39 -3.1078408311750714 2316 40-41 -3.2097744774905195 2316 42-43 -3.4131457794611393 2316 44-45 -3.059998159721264 2316 46-47 -2.825781948229917 2316 48-49 -3.01727633025596 2316 50-51 -3.434020214771337 2316 52-53 -4.417781683882154 2316 54-55 -3.6661399550083367 2316 56-57 -3.5405216134836195 2316 58-59 -3.5975856627520315 2316 60-61 -3.1647403270810237 2316 62-63 -3.269076006725925 2316 64-65 -3.24770461242489 2316 66-67 -3.4633198157563463 2316 68-69 -3.351969502134814 2316 70-71 -3.092769643093103 2316 72-73 -2.984977194720326 2316 74-75 -3.0616513520945254 2316 76-77 -3.318789758499225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47008.0 3 4343.0 4 5510.0 5 6650.0 6 7688.0 7 8770.0 8 10123.0 9 10128.0 10 12831.0 11 13991.0 12 11245.0 13 9802.0 14 9782.0 15 10177.0 16 10254.0 17 11032.0 18 11690.0 19 12761.0 20 13665.0 21 14927.0 22 16651.0 23 19268.0 24 22702.0 25 26645.0 26 31652.0 27 38253.0 28 46591.0 29 56998.0 30 69750.0 31 87401.0 32 111879.0 33 146907.0 34 201450.0 35 280695.0 36 407919.0 37 552828.0 38 500837.0 39 138803.0 40 1153.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.658885252807632 26.54459298719573 28.380130798941373 22.41639096105527 2 22.26615735391115 26.740886080210423 28.416605480033713 22.576351085844706 3 22.36607749920677 26.894052510471965 27.85379377094726 22.886076219374 4 22.31127165636058 26.942327363214257 28.25744892813493 22.48895205229023 5 22.37513110721312 27.14428409212484 28.064825871956707 22.41575892870534 6 22.63169278853733 26.8015492151774 27.88600650494403 22.68075149134124 7 22.438940496501864 26.718630168672515 28.081222246627103 22.761207088198525 8 22.32583532649127 26.83373863100047 27.894211976012777 22.946214066495482 9 22.210638060304674 26.738671417358134 28.1830771615174 22.867613360819796 10-11 22.778670221107628 26.86074434273424 27.869063647791698 22.491521788366438 12-13 22.86717517052433 26.49970586323724 27.91196228383448 22.721156682403958 14-15 22.47483899908899 26.916792769535615 27.97883971363026 22.629528517745136 16-17 22.800055455815812 26.75783105105768 27.97117697303894 22.47093652008757 18-19 22.655094230197157 26.733711470694356 27.77726392871219 22.833930370396295 20-21 22.63194648142716 26.840934241753555 27.883389776272633 22.64372950054665 22-23 22.81641800273221 27.028963877774103 27.419863149423186 22.734754970070497 24-25 22.620096957176553 27.12899397554922 27.429708213528443 22.821200853745786 26-27 22.71903798583952 27.175946712692735 27.444826550588175 22.660188750879573 28-29 22.974563691420872 26.991142184305755 27.359862343585135 22.67443178068824 30-31 22.695634292966357 27.06152462804805 27.40168210731333 22.84115897167226 32-33 22.590751093119135 27.27014586197812 27.36683060190333 22.772272442999412 34-35 22.6280870567544 26.94489637334625 27.545414202489958 22.881602367409393 36-37 22.486523183923108 27.153320165222954 27.672962263248557 22.687194387605377 38-39 22.518140760939577 27.048719043886955 27.759834539662204 22.67330565551127 40-41 22.398610608177208 27.04454782525085 27.636642443073946 22.920199123497994 42-43 22.374775837120556 27.164966381192468 27.465233401864698 22.995024379822283 44-45 22.618418927897523 26.939994677332884 27.66525389905364 22.776332495715952 46-47 22.51100100614436 26.8784208616709 27.557322479351708 23.05325565283303 48-49 22.590794919387754 26.897558941511896 27.48852819007814 23.023117949022215 50-51 22.41987739645231 27.237209711673138 27.359056509077583 22.983856382796965 52-53 22.513938254785646 26.995249483384026 27.466483929271828 23.0243283325585 54-55 22.563350576608293 27.11424846083062 27.397939973935912 22.92446098862517 56-57 22.612232708139214 27.145638659785483 27.273098464865974 22.969030167209333 58-59 22.603874090879852 27.047076433585943 27.392309612634243 22.956739862899965 60-61 22.54272701259141 27.125884592610344 27.343888142730076 22.987500252068166 62-63 22.582323122474474 27.014717536793842 27.430901910588112 22.972057430143575 64-65 22.559735574479177 27.024332894557457 27.203482118244153 23.21244941271921 66-67 22.410080935491614 27.159074241979788 27.345362543001144 23.085482279527454 68-69 22.57861256750907 26.903026816595876 27.48237250519211 23.035988110702952 70-71 22.395296891791368 26.781045659014318 27.580696090157026 23.24296135903729 72-73 22.514276298958926 26.513991456734498 27.628828568007414 23.34290367629916 74-75 22.352739287973407 26.72613757669077 27.41302542008675 23.508097715249065 76-77 22.332493333088728 26.50310288640984 27.729047386353972 23.435356394147462 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4344.0 1 2667.5 2 923.0 3 935.0 4 1015.0 5 1066.0 6 1151.5 7 1257.5 8 1383.0 9 1437.0 10 1501.0 11 1710.0 12 1872.0 13 1889.0 14 2060.5 15 2448.0 16 2911.0 17 3158.0 18 3488.5 19 4357.5 20 5570.5 21 7608.0 22 8971.0 23 10675.0 24 14573.5 25 19817.0 26 22866.0 27 26004.5 28 35325.5 29 44074.5 30 46641.0 31 52962.0 32 65106.5 33 76292.0 34 87893.0 35 94132.0 36 99740.5 37 110273.5 38 116374.0 39 117550.0 40 121544.5 41 128132.0 42 132974.5 43 135224.0 44 135924.5 45 138823.5 46 140898.5 47 141256.5 48 141738.0 49 141299.5 50 139432.0 51 134593.0 52 131183.0 53 128531.5 54 120730.5 55 108620.5 56 96156.0 57 90652.0 58 85889.0 59 73593.0 60 60064.0 61 54068.0 62 47483.5 63 35212.5 64 25164.5 65 20803.0 66 17488.0 67 12334.0 68 9066.5 69 6383.5 70 5129.0 71 4279.5 72 2890.5 73 2094.0 74 1837.0 75 1630.0 76 1133.0 77 708.5 78 574.0 79 487.0 80 356.5 81 278.0 82 196.0 83 149.0 84 132.5 85 117.5 86 95.0 87 71.0 88 65.0 89 63.5 90 57.5 91 47.0 92 48.5 93 43.5 94 37.0 95 36.5 96 36.0 97 35.0 98 34.5 99 84.0 100 133.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00919767298873385 2 0.012530163202043216 3 0.012230239082845375 4 0.014263058112964086 5 0.011730365550848968 6 0.009064373380201477 7 0.01492955615562596 8 0.01649582655588136 9 0.016462501653748268 10-11 0.018945206862663745 12-13 0.01512950556842452 14-15 0.007748039745944276 16-17 0.00573188316689211 18-19 0.017545560973073814 20-21 0.022627608548370596 22-23 0.020844726284250083 24-25 0.018945206862663745 26-27 0.023993929535827435 28-29 0.008164601022607947 30-31 0.007898001805543198 32-33 0.00951425955899824 34-35 0.01161372839338314 36-37 0.010947230350721267 38-39 0.013413273108570198 40-41 0.010314057210192488 42-43 0.014313045466163727 44-45 0.012780099968041417 46-47 0.023077494727167358 48-49 0.022910870216501893 50-51 0.036340805776138636 52-53 0.03967329598944801 54-55 0.028176204753530693 56-57 0.019911629024523463 58-59 0.015596054198287832 60-61 0.019278455883994683 62-63 0.02554353748501629 64-65 0.024860376991287868 66-67 0.027959592889665583 68-69 0.04180608972596599 70-71 0.05451953988974123 72-73 0.08691134476310826 74-75 0.08659475819284387 76-77 0.091443531453209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3000759.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.262817715200974 #Duplication Level Percentage of deduplicated Percentage of total 1 72.86410564146703 38.809475767626054 2 14.284757761736614 15.21692897538359 3 5.322974789868576 8.505499078061405 4 2.524363653953763 5.378188845896718 5 1.3906444488462324 3.7034820892776494 6 0.8846205463104033 2.8270429743151504 7 0.5683566831948953 2.1190594889958154 8 0.4045380115831861 1.7237467495860082 9 0.2853889499111909 1.368055765534714 >10 1.3535793164673753 12.669129243737606 >50 0.08020544507884475 2.9484315001399213 >100 0.03351601616770388 3.112427151298221 >500 0.0019449105926057856 0.720127440520479 >1k 0.0010038248216526398 0.8984049296265944 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4635 0.1544609213868891 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.332490213309366E-5 3.332490213309366E-5 2 0.0 0.0 0.0 9.997470639928098E-5 9.997470639928098E-5 3 0.0 0.0 0.0 4.332237277302176E-4 1.666245106654683E-4 4 9.997470639928098E-5 0.0 0.0 9.664221618597162E-4 1.666245106654683E-4 5 1.3329960853237464E-4 0.0 0.0 0.0018995194215863387 1.9994941279856195E-4 6 1.666245106654683E-4 0.0 0.0 0.0038656886474388646 1.9994941279856195E-4 7 1.666245106654683E-4 0.0 0.0 0.008497850043938883 2.3327431493165563E-4 8 1.666245106654683E-4 0.0 0.0 0.013329960853237463 2.3327431493165563E-4 9 1.666245106654683E-4 0.0 0.0 0.018328696173201514 2.3327431493165563E-4 10 2.3327431493165563E-4 0.0 0.0 0.023993929535827435 3.332490213309366E-4 11 3.332490213309366E-4 0.0 0.0 0.031092133690176386 3.332490213309366E-4 12 3.6657392346403026E-4 0.0 0.0 0.04075635530877355 3.332490213309366E-4 13 3.6657392346403026E-4 0.0 0.0 0.0541529659662772 3.332490213309366E-4 14 4.332237277302176E-4 0.0 0.0 0.07038219330509381 3.332490213309366E-4 15 4.332237277302176E-4 0.0 0.0 0.08721126888230611 3.332490213309366E-4 16 4.332237277302176E-4 0.0 0.0 0.10500676662137813 3.332490213309366E-4 17 4.332237277302176E-4 0.0 0.0 0.12253566514338539 3.332490213309366E-4 18 4.6654862986331125E-4 0.0 0.0 0.14073106170805452 3.998988255971239E-4 19 4.998735319964049E-4 0.0 0.0 0.16195902436683518 3.998988255971239E-4 20 6.331731405287796E-4 0.0 0.0 0.18478658232800435 3.998988255971239E-4 21 6.331731405287796E-4 0.0 0.0 0.20891381147236415 4.998735319964049E-4 22 7.997976511942478E-4 0.0 0.0 0.23514050945110887 5.331984341294985E-4 23 9.330972597266225E-4 0.0 0.0 0.26213368017891475 5.998482383956859E-4 24 9.330972597266225E-4 0.0 0.0 0.29112634503470625 5.998482383956859E-4 25 9.330972597266225E-4 0.0 0.0 0.3213853561715553 6.664980426618732E-4 26 9.330972597266225E-4 0.0 0.0 0.3523441902531993 6.998229447949669E-4 27 9.330972597266225E-4 0.0 0.0 0.3838362227689728 6.998229447949669E-4 28 9.330972597266225E-4 0.0 0.0 0.4150949809698146 8.331225533273415E-4 29 9.330972597266225E-4 0.0 0.0 0.44871980722210614 8.331225533273415E-4 30 9.997470639928098E-4 0.0 0.0 0.4894428376287466 8.664474554604352E-4 31 9.997470639928098E-4 0.0 0.0 0.529466045090592 8.664474554604352E-4 32 0.001066396868258997 0.0 0.0 0.5713221221697578 8.664474554604352E-4 33 0.001066396868258997 0.0 0.0 0.614477870432114 9.330972597266225E-4 34 0.0010997217703920907 0.0 0.0 0.6632988520570963 9.330972597266225E-4 35 0.0010997217703920907 0.0 0.0 0.7119865340735461 9.330972597266225E-4 36 0.0010997217703920907 0.0 0.0 0.7627070351201146 9.330972597266225E-4 37 0.0011330466725251844 0.0 0.0 0.8149604816648055 9.330972597266225E-4 38 0.0011330466725251844 0.0 0.0 0.8690801227289495 9.330972597266225E-4 39 0.001166371574658278 0.0 0.0 0.9244661100741512 9.330972597266225E-4 40 0.001166371574658278 0.0 0.0 0.985250731564914 9.997470639928098E-4 41 0.001166371574658278 0.0 0.0 1.0504675650393784 0.001066396868258997 42 0.0011996964767913719 0.0 0.0 1.1198500112804795 0.001066396868258997 43 0.0011996964767913719 0.0 0.0 1.2011294475830947 0.0011996964767913719 44 0.0011996964767913719 0.0 0.0 1.2830753819283722 0.0011996964767913719 45 0.0011996964767913719 0.0 0.0 1.3650213162736495 0.0011996964767913719 46 0.0012330213789244655 0.0 0.0 1.453832180458344 0.0011996964767913719 47 0.0012330213789244655 0.0 0.0 1.5401436769830565 0.0012663462810575592 48 0.0012330213789244655 0.0 0.0 1.6336200274663844 0.0012996711831906528 49 0.0012330213789244655 0.0 0.0 1.7306621424779531 0.0014996205959892147 50 0.0012330213789244655 0.0 0.0 1.8299703508345722 0.0014996205959892147 51 0.0012330213789244655 0.0 0.0 1.937043261388202 0.0015329454981223083 52 0.0012330213789244655 0.0 0.0 2.0503479286407207 0.0015329454981223083 53 0.0012330213789244655 0.0 0.0 2.177082531452876 0.0015329454981223083 54 0.0012330213789244655 0.0 0.0 2.310848688615114 0.001566270400255402 55 0.0012330213789244655 0.0 0.0 2.449280332075985 0.001566270400255402 56 0.0014329707917230274 0.0 0.0 2.593877082431478 0.001566270400255402 57 0.0014329707917230274 0.0 0.0 2.7385071576891047 0.0016329202045215893 58 0.001466295693856121 0.0 0.0 2.8963005692893034 0.001766219813053964 59 0.001466295693856121 0.0 0.0 3.0516279381316527 0.0017995447151870577 60 0.001466295693856121 0.0 0.0 3.2130204391622255 0.0017995447151870577 61 0.0016329202045215893 0.0 0.0 3.3877429010460354 0.0017995447151870577 62 0.0016329202045215893 0.0 0.0 3.5689303939436656 0.0017995447151870577 63 0.0016329202045215893 0.0 0.0 3.7602153321876233 0.001966169225852526 64 0.0016329202045215893 0.0 0.0 3.9552993092747535 0.001966169225852526 65 0.0016329202045215893 0.0 0.0 4.167079062330564 0.001966169225852526 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGCG 110 2.6195865E-5 22.593504 52 ACTACGG 135 0.0026707964 15.787749 67 TCGAACT 920 0.0 14.664238 24 ACGCGAA 170 7.3454896E-4 14.618107 54 AACGCGA 200 1.5551646E-4 14.20092 53 CCGGTTA 225 2.662735E-5 14.196423 34 GTAACGT 200 1.5602383E-4 14.1954775 2 CGAACTC 1015 0.0 13.989189 25 TAAGGCG 1045 0.0 13.927972 17 GGTAACG 205 1.9230203E-4 13.850169 1 ATACGTA 155 0.0067282044 13.738475 1 GCCTCGA 1015 0.0 12.942157 21 ATAACGC 195 0.00206683 12.745478 51 CTCGAAC 1070 0.0 12.608925 23 CGTCGTG 310 3.0184347E-6 12.592342 4 CGCCGTA 230 5.048342E-4 12.35563 67 TTGGACG 320 4.2829197E-6 12.204526 45 CCGGGGT 730 0.0 12.159336 48 CCGTATC 265 1.2244254E-4 12.064827 69 CGTGCGC 540 9.094947E-11 11.835087 46 >>END_MODULE