FastQCFastQC Report
Thu 26 May 2016
SRR936015_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936015_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3000759
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52880.17622208247979929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38200.12730112614841776No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62600.038.1154821
CGTATCA11400.036.54909100-101
GTATCAA118700.031.7811741
TACGCAG7850.028.434462100-101
CCTGTCG8250.027.05266296-97
CGGTATC10750.025.470222100-101
AGGTATC20000.025.297194100-101
GGGTATC11150.025.090332100-101
GCGTATC3450.025.01683100-101
ATCAACG149350.024.9759123
TCAACGC150550.024.8163494
TATCAAC152350.024.5626052
CGTACAT18100.024.006435100-101
CAACGCA156200.023.9187035
GCGTACA5900.023.708237100-101
CGAGTAC10100.022.984055100-101
AACGCAG162800.022.9490266
TTATACG8050.022.91915396-97
CGTATCG650.00204859422.893387100-101
ACGTATC4950.022.847137100-101
Produced by FastQC (version 0.11.5)