FastQCFastQC Report
Thu 26 May 2016
SRR936014_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936014_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028710
Sequences flagged as poor quality0
Sequence length77
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45400.1498988018001063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGCG12200.016.0026117
GCGTATA2452.1130836E-715.93834643
TAACCGG2805.7771103E-815.21588659
GTAACGT2951.0993426E-714.43742752
GGTAACG2757.8371704E-714.1968041
CGAACTC9350.014.04682225
GGCGGAA14100.013.84737420
TAACGTT3353.4708137E-813.7730193
TCGAACT9400.013.59537824
GACGCGA6800.013.5807969
GTGCGAC1850.001387607313.43828467
CTAACCG3253.5694575E-713.10820558
GCCTCGA9550.013.010550521
ATCGTAC2203.4952935E-412.90618533
CGCGACC7500.012.78683571
CTCGAAC10300.012.75229523
AGGCGGA15600.012.74345719
GTAGACC16150.012.5272128
TCGCGCT2000.002504116612.42384437
GCGGAAG16100.012.347921
Produced by FastQC (version 0.11.5)