##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936014_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3028710 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.478426788962956 40.0 37.0 41.0 32.0 41.0 2 37.363169468189426 40.0 37.0 41.0 32.0 41.0 3 37.23960068808172 40.0 37.0 41.0 31.0 41.0 4 37.14771899587613 40.0 37.0 41.0 31.0 41.0 5 37.07565597234466 40.0 37.0 41.0 31.0 41.0 6 36.990004325273794 39.0 36.0 41.0 31.0 41.0 7 36.85700908967844 39.0 36.0 41.0 31.0 41.0 8 36.734806897986275 39.0 36.0 41.0 30.0 41.0 9 36.912834507100385 39.0 36.0 41.0 31.0 41.0 10-11 37.14906263722839 40.0 37.0 41.0 31.0 41.0 12-13 37.09707317636882 40.0 37.0 41.0 31.0 41.0 14-15 36.96388594484121 40.0 37.0 41.0 31.0 41.0 16-17 36.827608288677354 40.0 36.0 41.0 30.0 41.0 18-19 36.68894083619759 40.0 36.0 41.0 30.0 41.0 20-21 36.511724958810845 40.0 36.0 41.0 30.0 41.0 22-23 36.28060131210978 39.5 36.0 41.0 30.0 41.0 24-25 36.083678694889905 39.0 35.0 41.0 29.0 41.0 26-27 35.134039409517584 38.0 34.0 40.0 27.0 40.5 28-29 35.16374991332944 38.5 34.5 40.0 27.5 40.5 30-31 35.576414050866546 39.0 35.0 40.5 28.0 41.0 32-33 35.457182100630305 39.0 35.0 41.0 28.0 41.0 34-35 35.213062491952016 38.5 35.0 40.0 27.0 41.0 36-37 34.91157010740547 38.0 34.0 40.0 26.5 41.0 38-39 34.60761429783638 38.0 34.0 40.0 26.0 41.0 40-41 34.25842718517124 37.0 34.0 40.0 26.0 41.0 42-43 33.89039673656441 36.5 33.5 39.5 25.5 41.0 44-45 33.52038409091659 36.0 33.0 39.0 25.0 41.0 46-47 33.10162494923581 35.5 33.0 39.0 24.0 40.0 48-49 32.68454672121134 35.0 32.5 38.0 22.5 40.0 50-51 32.23972367773739 35.0 32.0 37.0 21.5 39.5 52-53 31.772431992498458 35.0 32.0 37.0 20.0 39.0 54-55 31.317949721168418 35.0 31.0 36.0 18.5 39.0 56-57 30.893453978756632 35.0 31.0 36.0 17.5 37.5 58-59 30.477811015250715 34.0 31.0 35.0 12.0 37.0 60-61 30.120565521294544 34.0 30.5 35.0 7.5 36.5 62-63 29.770492883108652 34.0 30.0 35.0 4.5 36.0 64-65 29.406224267097215 34.0 30.0 35.0 2.0 36.0 66-67 29.067454130636477 34.0 29.0 35.0 2.0 35.5 68-69 28.725213209584275 34.0 29.0 35.0 2.0 35.0 70-71 28.29475981523487 34.0 29.0 35.0 2.0 35.0 72-73 27.841291176771627 34.0 29.0 35.0 2.0 35.0 74-75 27.295070508566354 34.0 27.0 35.0 2.0 35.0 76-77 25.49068250179119 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8127542606141702 1101 2 0.7611528259208526 1101 3 0.8545426971735495 1101 4 0.7388365561270618 1101 5 0.7603064868445557 1101 6 0.8254619025651806 1101 7 0.7660338138403233 1101 8 0.801375652253796 1101 9 0.7217013285288587 1101 10-11 0.884695371166778 1101 12-13 1.0057920704228138 1101 14-15 1.0267492529697364 1101 16-17 1.1327401102039758 1101 18-19 0.9875615695444964 1101 20-21 0.9902349573004514 1101 22-23 0.9756751018419365 1101 24-25 1.035270838843779 1101 26-27 0.8990384105338691 1101 28-29 0.9941114815401519 1101 30-31 0.9301575404385503 1101 32-33 1.0186999425884906 1101 34-35 1.1053160044491577 1101 36-37 1.0403971576637474 1101 38-39 1.0434392769076055 1101 40-41 1.0500838044636964 1101 42-43 0.9663065650077414 1101 44-45 0.9542327976713523 1101 46-47 0.904931708675548 1101 48-49 0.9265311064673085 1101 50-51 0.9715487158655947 1101 52-53 1.0313543624536585 1101 54-55 1.099735095966114 1101 56-57 1.1295163675979971 1101 58-59 1.1491327667824933 1101 60-61 0.9406618535809841 1101 62-63 0.8817354052648234 1101 64-65 0.8798359976071985 1101 66-67 1.107382530903216 1101 68-69 1.026853491261214 1101 70-71 1.0676069745421906 1101 72-73 0.9618013631545459 1101 74-75 0.9823269674643065 1101 76-77 1.3943225418550185 1102 1 0.7395259462854469 1102 2 0.5995513140633548 1102 3 0.6307744245189895 1102 4 0.5844783356054251 1102 5 0.6901092736666712 1102 6 0.7046428579735888 1102 7 0.7414208595064835 1102 8 0.7250554797355733 1102 9 0.7855078344362383 1102 10-11 0.8511042737804928 1102 12-13 0.959320431202876 1102 14-15 0.8796048883885916 1102 16-17 0.8297658546092421 1102 18-19 0.8577932712766767 1102 20-21 0.9417563999187593 1102 22-23 0.7883808579856151 1102 24-25 0.8835044222571824 1102 26-27 0.7576971869923383 1102 28-29 0.8846662637892067 1102 30-31 0.9090245646253123 1102 32-33 0.8390009738597968 1102 34-35 0.8803319457260912 1102 36-37 0.8032028700564027 1102 38-39 0.8956243511865623 1102 40-41 0.9309990159314481 1102 42-43 0.9202098423833718 1102 44-45 0.780305499424685 1102 46-47 0.8327345266703929 1102 48-49 0.7696730614163627 1102 50-51 0.7957334323531029 1102 52-53 0.8568380088330052 1102 54-55 0.9570075794706305 1102 56-57 0.993022106308775 1102 58-59 1.080644990612221 1102 60-61 0.9492876653749391 1102 62-63 0.9066858987023174 1102 64-65 0.921804319165215 1102 66-67 0.8178266459933283 1102 68-69 0.7449720542539993 1102 70-71 0.7016824206869821 1102 72-73 0.7431318172131434 1102 74-75 0.6603121806703811 1102 76-77 0.8433882882180121 1103 1 0.4463388500190817 1103 2 0.446880975316013 1103 3 0.4781572471037663 1103 4 0.43112120727288783 1103 5 0.3536701959169051 1103 6 0.42117038325679346 1103 7 0.4762583028243128 1103 8 0.26405176441441114 1103 9 0.3688496771724843 1103 10-11 0.4529091548523283 1103 12-13 0.4880613760314958 1103 14-15 0.38050447015673683 1103 16-17 0.35149498616748787 1103 18-19 0.3650045619212392 1103 20-21 0.38881672021235403 1103 22-23 0.29081193647240156 1103 24-25 0.42531830648943014 1103 26-27 0.519155093937691 1103 28-29 0.48018224738496684 1103 30-31 0.5194599383127709 1103 32-33 0.5843618154575339 1103 34-35 0.6142939403666858 1103 36-37 0.592986613086083 1103 38-39 0.48303891000967525 1103 40-41 0.6281666134435824 1103 42-43 0.6417624841931868 1103 44-45 0.5145268391274556 1103 46-47 0.49798290512738674 1103 48-49 0.3365392968333438 1103 50-51 0.4248190349230967 1103 52-53 0.4882208564809538 1103 54-55 0.38237562868764385 1103 56-57 0.4516891705921857 1103 58-59 0.48434000669328015 1103 60-61 0.39839097664060574 1103 62-63 0.23197842554071357 1103 64-65 0.20629483828708928 1103 66-67 0.37799005002601405 1103 68-69 0.3795798269106925 1103 70-71 0.2807632798563482 1103 72-73 0.2558678723740435 1103 74-75 0.23121378217754085 1103 76-77 0.3374893384137643 1104 1 0.3667065843153736 1104 2 0.32508699412070996 1104 3 0.4566033187188978 1104 4 0.43620739119655383 1104 5 0.4314691473219554 1104 6 0.4748459208687308 1104 7 0.4221597832513666 1104 8 0.4370283289963268 1104 9 0.2220899485873815 1104 10-11 0.43766679237003103 1104 12-13 0.42053011304327015 1104 14-15 0.4899932673652003 1104 16-17 0.5266587236521829 1104 18-19 0.40916732255406885 1104 20-21 0.27709963911952684 1104 22-23 0.2809800342721829 1104 24-25 0.2984656141027884 1104 26-27 0.20432518547290357 1104 28-29 0.045895502311125824 1104 30-31 0.34117255116192524 1104 32-33 0.25874660286371665 1104 34-35 0.3615755135024301 1104 36-37 0.2950059766527602 1104 38-39 0.37854632352648565 1104 40-41 0.4756601779560299 1104 42-43 0.47655932922460664 1104 44-45 0.4137314937147565 1104 46-47 0.3150491921208953 1104 48-49 0.2781251053617453 1104 50-51 0.3037374805472979 1104 52-53 0.23661330917192558 1104 54-55 0.3021300342346258 1104 56-57 0.31607807302120605 1104 58-59 0.20760167936666107 1104 60-61 0.12324920753008328 1104 62-63 0.15328690468557582 1104 64-65 0.176863103906026 1104 66-67 0.3215840171709772 1104 68-69 0.07524474748898058 1104 70-71 0.06338543245481887 1104 72-73 0.12389642125254952 1104 74-75 0.22945768588012072 1104 76-77 0.17786236317336446 1105 1 0.29153890680017724 1105 2 0.41117100563576514 1105 3 0.40217839547143797 1105 4 0.43609393263976415 1105 5 0.3966506138074024 1105 6 0.24543370735158732 1105 7 0.48776294396165554 1105 8 0.3988785810957083 1105 9 0.4100840566489481 1105 10-11 0.46489032312126 1105 12-13 0.6411234933887684 1105 14-15 0.5566279323936314 1105 16-17 0.5556670523799667 1105 18-19 0.45683563061420784 1105 20-21 0.4263219538480243 1105 22-23 0.49426393604474583 1105 24-25 0.519344913964666 1105 26-27 0.4145683386245693 1105 28-29 0.6715865321273995 1105 30-31 0.7820415685950053 1105 32-33 0.7663666099953943 1105 34-35 0.665500349382846 1105 36-37 0.69898090033508 1105 38-39 0.6579262586772145 1105 40-41 0.7681699761104923 1105 42-43 0.7879977307160928 1105 44-45 0.5957327563859423 1105 46-47 0.5274698007805796 1105 48-49 0.3706301991370182 1105 50-51 0.43445972530309973 1105 52-53 0.43063712677167487 1105 54-55 0.3855760354727842 1105 56-57 0.3772743373488048 1105 58-59 0.48281043125137657 1105 60-61 0.41091606474387277 1105 62-63 0.29630003188012566 1105 64-65 0.2690875162341868 1105 66-67 0.43917590476556967 1105 68-69 0.27511168076349435 1105 70-71 0.2780649337748109 1105 72-73 -0.1362041575837516 1105 74-75 -0.32621923475186065 1105 76-77 -0.5086332617539924 1106 1 0.03497105372861142 1106 2 0.1065228992393088 1106 3 0.024429136446741495 1106 4 0.0771791764649663 1106 5 0.12797281773394076 1106 6 0.09604092711092704 1106 7 -0.07194938029419973 1106 8 0.06836276095637572 1106 9 -0.03302555703059795 1106 10-11 -0.102619949202996 1106 12-13 0.20176891454139678 1106 14-15 0.17891256823910595 1106 16-17 0.20588693838376315 1106 18-19 0.21803608659901386 1106 20-21 0.26179377145410143 1106 22-23 0.2811921691036403 1106 24-25 0.17591889749854062 1106 26-27 0.04687045388738653 1106 28-29 0.0926548031850345 1106 30-31 0.1658604412992517 1106 32-33 0.09705306978006689 1106 34-35 0.020641780668476883 1106 36-37 0.01974665081007032 1106 38-39 0.14340729730925972 1106 40-41 0.14618142639548637 1106 42-43 0.22296472248303445 1106 44-45 0.17343611294072048 1106 46-47 0.17460479761402325 1106 48-49 0.005303232702566163 1106 50-51 0.00238337318150883 1106 52-53 0.0014233965626821998 1106 54-55 -0.054104572506947335 1106 56-57 0.003451056791494267 1106 58-59 -0.14277654784931926 1106 60-61 -0.3078067724948852 1106 62-63 -0.08191169706223889 1106 64-65 -0.10391372505776886 1106 66-67 -0.2653129616055523 1106 68-69 -0.2037521064436021 1106 70-71 -0.32496467990473477 1106 72-73 -0.4667012316812844 1106 74-75 -0.3104476324719414 1106 76-77 -0.5231797342049127 1107 1 -0.2167330117213595 1107 2 -0.08226391007472245 1107 3 0.058426166025093096 1107 4 -0.11241025084780887 1107 5 -0.1100587492893439 1107 6 -0.48720690171223424 1107 7 -0.3248882349197544 1107 8 -0.2284052189051735 1107 9 -0.3181197798122213 1107 10-11 -0.18389400835970093 1107 12-13 -0.176451299429651 1107 14-15 -0.40979167342232614 1107 16-17 -0.33213395526003353 1107 18-19 -0.2751573180580067 1107 20-21 -0.48591547964721116 1107 22-23 -0.4638669120794887 1107 24-25 -0.4746419080395299 1107 26-27 -0.769437060272459 1107 28-29 -0.6683954170792816 1107 30-31 -0.368154662539645 1107 32-33 -0.250271486544662 1107 34-35 -0.43955275677645744 1107 36-37 -0.4523565084095722 1107 38-39 -0.4052024950129507 1107 40-41 -0.5566790393062178 1107 42-43 -0.3575954285553564 1107 44-45 -0.4936360078899895 1107 46-47 -0.45270581283279654 1107 48-49 -0.3141779000853475 1107 50-51 -0.339356740097287 1107 52-53 -0.34210103389672497 1107 54-55 -0.38727960082664836 1107 56-57 -0.35322650142122214 1107 58-59 -0.40920106641445386 1107 60-61 -0.4268113288196176 1107 62-63 -0.38273163412958056 1107 64-65 -0.4652596281414709 1107 66-67 -0.521390544991327 1107 68-69 -0.7520210051220175 1107 70-71 -0.6677285817297793 1107 72-73 -0.5475495136196109 1107 74-75 -0.6776859962227277 1107 76-77 -0.7265742716498487 1108 1 0.10795812139778604 1108 2 0.2652651548784064 1108 3 0.30657820912844613 1108 4 0.35496965265544134 1108 5 0.2798435946762936 1108 6 0.24845245593298415 1108 7 0.32948310090880284 1108 8 0.22273088125712803 1108 9 0.24448441790674735 1108 10-11 0.2851841109473767 1108 12-13 0.3199362829624519 1108 14-15 0.27619918478045946 1108 16-17 0.2385355599376524 1108 18-19 0.11947187607269427 1108 20-21 0.19566595190523373 1108 22-23 0.06556790845201732 1108 24-25 0.18621107794967173 1108 26-27 0.03629215814804354 1108 28-29 0.2293445274958117 1108 30-31 0.3106286117253134 1108 32-33 0.31333121514347795 1108 34-35 0.24674724432762218 1108 36-37 0.2569491069504153 1108 38-39 0.1974439890385824 1108 40-41 0.1661087948165374 1108 42-43 0.18212943426247818 1108 44-45 0.1454120527903484 1108 46-47 0.01512745425562656 1108 48-49 0.09262448583540106 1108 50-51 0.04021760877048308 1108 52-53 0.17377773240228223 1108 54-55 0.1768730716033282 1108 56-57 0.16704444025014098 1108 58-59 0.0974779383662181 1108 60-61 0.08760590920937616 1108 62-63 0.06505426785004076 1108 64-65 0.006388931583831692 1108 66-67 0.13969084791825992 1108 68-69 0.09323917175188612 1108 70-71 0.12703296420554366 1108 72-73 0.021100221952803366 1108 74-75 0.018945450234831185 1108 76-77 0.19296507782516414 1109 1 0.11081105372861089 1109 2 0.10556289923930962 1109 3 4.291364467405856E-4 1109 4 0.010619176464963687 1109 5 0.03517281773394387 1109 6 0.00484092711092643 1109 7 0.0490106197057969 1109 8 0.11124276095637953 1109 9 0.06233444296940149 1109 10-11 -0.033019949203001886 1109 12-13 0.0020889145413960364 1109 14-15 0.18387256823910292 1109 16-17 0.1290869383837716 1109 18-19 -0.017803913400989302 1109 20-21 0.12131377145410482 1109 22-23 0.10951216910364536 1109 24-25 0.030638897498540985 1109 26-27 -0.1628895461126092 1109 28-29 0.12833480318503376 1109 30-31 0.22538044129925083 1109 32-33 0.14073306978007594 1109 34-35 0.12560178066847527 1109 36-37 0.3103066508100696 1109 38-39 0.23476729730926138 1109 40-41 0.15930142639548706 1109 42-43 0.2389647224830327 1109 44-45 0.25935611294072203 1109 46-47 0.30420479761401964 1109 48-49 0.2835432327025629 1109 50-51 0.18350337318150167 1109 52-53 0.24478339656268489 1109 54-55 0.31837542749305214 1109 56-57 0.3973710567914921 1109 58-59 0.44794345215068176 1109 60-61 0.45603322750511666 1109 62-63 0.30464830293776046 1109 64-65 0.39912627494222974 1109 66-67 0.36140703839444654 1109 68-69 0.36760789355639645 1109 70-71 0.39999532009526817 1109 72-73 0.24433876831871615 1109 74-75 0.08459236752806021 1109 76-77 0.05458026579508868 1110 1 -0.3956584854992826 1110 2 -0.2908425802127468 1110 3 -0.5463273268160265 1110 4 -0.3964387562872105 1110 5 -0.46143480368573364 1110 6 -0.43442183752169683 1110 7 -0.32881075015721706 1110 8 -0.6278248355567015 1110 9 -0.5185665782012094 1110 10-11 -0.49135464409715013 1110 12-13 -0.49897061223320094 1110 14-15 -0.3918185401046088 1110 16-17 -0.5697113181542122 1110 18-19 -0.5796334526288831 1110 20-21 -0.5871726046355619 1110 22-23 -0.48015622442063943 1110 24-25 -0.5400427961502672 1110 26-27 -0.5948422235721367 1110 28-29 -0.3234630050341494 1110 30-31 -0.18280082893736704 1110 32-33 -0.10799978202565796 1110 34-35 -0.2714606352717581 1110 36-37 -0.20385713499317148 1110 38-39 -0.21628998787131337 1110 40-41 -0.23265488742768525 1110 42-43 -0.22465066979592052 1110 44-45 -0.29512577996089817 1110 46-47 -0.29628327212445527 1110 48-49 -0.21443031648797017 1110 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-0.23346967894968174 2314 7 -0.26643614797097115 2314 8 -0.4080197642961494 2314 9 -0.22379091056595257 2314 10-11 -0.43833515122320676 2314 12-13 -0.5074328026303263 2314 14-15 -0.44554717923564624 2314 16-17 -0.46184109191926126 2314 18-19 -0.43146512552220884 2314 20-21 -0.4267542083438727 2314 22-23 -0.3564210127145415 2314 24-25 -0.2782229711883204 2314 26-27 -0.22609914207220783 2314 28-29 -0.3672333281281013 2314 30-31 -0.5142531950643914 2314 32-33 -0.6521744049674041 2314 34-35 -0.6169899870082958 2314 36-37 -0.9753325916141762 2314 38-39 -0.5318816925897281 2314 40-41 -0.5179522099681577 2314 42-43 -0.34650275226444194 2314 44-45 -0.3731772708976564 2314 46-47 -0.31549075794153225 2314 48-49 -0.15191085820652717 2314 50-51 -0.06241349550536057 2314 52-53 0.013218901613189615 2314 54-55 -0.22737144119381725 2314 56-57 -0.09842510482466693 2314 58-59 -0.11943270946547813 2314 60-61 -0.033101873504985946 2314 62-63 -0.09573952534507058 2314 64-65 0.04477324463919885 2314 66-67 -0.03204887069646034 2314 68-69 0.05293243901094513 2314 70-71 0.08547784534779268 2314 72-73 0.24530538448033212 2314 74-75 0.19746110490179802 2314 76-77 0.296903902158725 2315 1 -0.9130156618432466 2315 2 -0.7197618089967719 2315 3 -0.5675000132698358 2315 4 -0.5116843113643128 2315 5 -0.5029450082080373 2315 6 -0.5420160252065145 2315 7 -0.9672176163728992 2315 8 -0.8645632543820696 2315 9 -0.5887346333893788 2315 10-11 -0.7549826300966274 2315 12-13 -0.6265294249050797 2315 14-15 -0.5789962813774352 2315 16-17 -0.5149877531800868 2315 18-19 -0.5483077346747507 2315 20-21 -0.4987130641577622 2315 22-23 -0.6078462036872878 2315 24-25 -0.7170500654891185 2315 26-27 -0.6217304930949368 2315 28-29 -0.6482190881120644 2315 30-31 -0.6921922429288259 2315 32-33 -0.5771435024106566 2315 34-35 -0.570069096290517 2315 36-37 -0.6672979373860031 2315 38-39 -0.672432435935157 2315 40-41 -0.6358087303234186 2315 42-43 -0.6620186986573486 2315 44-45 -0.6655174048985515 2315 46-47 -0.6758029916490642 2315 48-49 -0.5341646966072062 2315 50-51 -0.5500849962749825 2315 52-53 -0.6744289275453532 2315 54-55 -0.7108227852445275 2315 56-57 -0.8171232146323142 2315 58-59 -0.7395771347116096 2315 60-61 -0.7173378962027854 2315 62-63 -0.7166533042646428 2315 64-65 -0.4747007340607716 2315 66-67 -0.5114885134561682 2315 68-69 -0.7599550007417015 2315 70-71 -0.6476460536959969 2315 72-73 -0.6017404847656458 2315 74-75 -0.6649178425419606 2315 76-77 -0.5399849092632643 2316 1 -1.6420638120823554 2316 2 -1.3293010447513538 2316 3 -1.4051928316590576 2316 4 -2.5350675757715138 2316 5 -1.8427071667079105 2316 6 -1.8840334408392891 2316 7 -1.5776319007142732 2316 8 -1.267162902209705 2316 9 -1.6206809751558424 2316 10-11 -1.4217668648000412 2316 12-13 -1.9110676782240645 2316 14-15 -3.1302303955882067 2316 16-17 -2.2831339873260745 2316 18-19 -1.7902550374772233 2316 20-21 -1.6277589317742098 2316 22-23 -1.9579009152993123 2316 24-25 -1.8998304918441207 2316 26-27 -1.8719350536972073 2316 28-29 -2.3095479194909743 2316 30-31 -2.421926412065204 2316 32-33 -2.4622382876683915 2316 34-35 -2.3936964845979603 2316 36-37 -2.6459954262027665 2316 38-39 -2.6086274907109654 2316 40-41 -2.2342845090382006 2316 42-43 -2.505749832164966 2316 44-45 -2.4759868353284347 2316 46-47 -2.4759746811879992 2316 48-49 -2.1867128233067703 2316 50-51 -2.3502197228900634 2316 52-53 -2.3780322549347908 2316 54-55 -2.4743253737516007 2316 56-57 -2.3696414986344116 2316 58-59 -2.3817760966630104 2316 60-61 -2.2154020117014213 2316 62-63 -2.275363396789974 2316 64-65 -2.183833196080716 2316 66-67 -2.10278706506724 2316 68-69 -2.070665338648972 2316 70-71 -1.9962823617405938 2316 72-73 -2.10675058212858 2316 74-75 -2.2985972396286876 2316 76-77 -2.700554638911253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 47857.0 3 4356.0 4 5665.0 5 7156.0 6 8169.0 7 9135.0 8 10522.0 9 10826.0 10 13053.0 11 14457.0 12 11622.0 13 10361.0 14 10178.0 15 10602.0 16 10809.0 17 11431.0 18 12264.0 19 13163.0 20 14118.0 21 15732.0 22 17476.0 23 19781.0 24 23346.0 25 27238.0 26 32536.0 27 39212.0 28 47408.0 29 57724.0 30 71854.0 31 88601.0 32 112619.0 33 146287.0 34 201909.0 35 279492.0 36 409413.0 37 559191.0 38 505332.0 39 136819.0 40 996.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.64616752085277 26.54501365971365 28.331309305180525 22.477509514253054 2 22.25461497694369 26.73594291478658 28.4287862953222 22.580655812947526 3 22.324316452782814 26.862156608352315 27.869930790854582 22.94359614801029 4 22.263556644890965 26.975581758607415 28.315114264635856 22.445747331865768 5 22.43380561494024 27.08998315214125 28.01631818479017 22.459893048128343 6 22.643944955128188 26.768083537491254 27.898390281257463 22.6895812261231 7 22.47023485192223 26.668595201251172 28.07867100190674 22.782498944919862 8 22.27234104665674 26.84571404794433 27.883282691635152 22.998662213763776 9 22.222724913049163 26.769563442737937 28.122106317128488 22.88560532708441 10-11 22.806083299854805 26.87906995253232 27.84386243273541 22.47098431487746 12-13 22.89907863016413 26.497093887256035 27.882913378025826 22.72091410455401 14-15 22.438259619981984 26.938822276991026 27.956244172009264 22.666673931017723 16-17 22.795494630297082 26.771942528354202 27.950092446160575 22.482470395188145 18-19 22.64918408471947 26.718680732643797 27.808251860131637 22.823883322505097 20-21 22.620331688851298 26.866569112740823 27.875976522997792 22.637122675410087 22-23 22.853397219878577 27.0197488017145 27.40927620749038 22.71757777091654 24-25 22.63623297913375 27.131571230046553 27.408944640243483 22.823251150576212 26-27 22.71002789672927 27.168072510362208 27.46246093533295 22.659438657575574 28-29 22.966789979819833 26.927851486650763 27.41781861029758 22.687539923231824 30-31 22.70198768652361 27.048009878253726 27.457292385710875 22.79271004951179 32-33 22.58567119635969 27.255459067908085 27.431474682627883 22.727395053104345 34-35 22.62041248429029 26.930172613626684 27.54394921116293 22.905465690920103 36-37 22.493033923722436 27.108780748022117 27.696198009098122 22.701987319157325 38-39 22.556517233675898 27.01125597650232 27.777846575216707 22.65438021460508 40-41 22.421348450959048 27.038809856814368 27.592825974217238 22.947015718009347 42-43 22.375621872064627 27.170280050676045 27.477255469361815 22.976842607897517 44-45 22.65956161010669 26.901113939366724 27.639133444203303 22.800191006323285 46-47 22.523572561020515 26.867567993295033 27.569287792132823 23.039571653551626 48-49 22.595806003471935 26.91568252748011 27.479154015099656 23.009357453948294 50-51 22.462082937912406 27.226140698898888 27.352846031636208 22.9589303315525 52-53 22.51856221251731 26.99785090846759 27.472619477368156 23.010967401646944 54-55 22.589027202632515 27.07531987507179 27.42618096096439 22.909471961331302 56-57 22.623558323861403 27.120716084492475 27.28435866835125 22.97136692329487 58-59 22.6612326794133 27.077681988240965 27.342176909274723 22.91890842307101 60-61 22.56413973685779 27.095262776471785 27.358329030629253 22.98226845604117 62-63 22.567900565906164 27.017837495712893 27.421324908342225 22.992937030038718 64-65 22.5885427700577 27.028465816763514 27.19639453572828 23.18659687745051 66-67 22.434554256289136 27.09606194472542 27.37780450504092 23.091579293944527 68-69 22.605739046288544 26.87740991560032 27.488139293328118 23.028711744783024 70-71 22.36676016714463 26.7909922403743 27.552898355146993 23.289349237334076 72-73 22.50839462354241 26.527885909438336 27.65916746412906 23.30455200289019 74-75 22.363282847231755 26.67653292533413 27.42942163932216 23.53076258811195 76-77 22.2939859194835 26.45886491840057 27.751255772920903 23.495893389195025 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4225.0 1 2571.0 2 899.0 3 927.5 4 974.0 5 1030.0 6 1145.5 7 1255.0 8 1322.0 9 1339.0 10 1464.5 11 1645.5 12 1801.0 13 1901.0 14 2086.5 15 2516.0 16 2931.5 17 3103.0 18 3549.5 19 4369.0 20 5510.5 21 7550.5 22 8822.0 23 10553.0 24 14601.5 25 19855.5 26 22792.0 27 26197.5 28 35864.0 29 44635.5 30 47146.0 31 53718.5 32 65974.0 33 77383.0 34 88982.0 35 94855.0 36 100562.5 37 110926.0 38 117206.0 39 118830.0 40 122781.5 41 129386.0 42 134544.0 43 137049.0 44 137628.5 45 139867.0 46 141924.0 47 142893.0 48 143464.0 49 142965.5 50 140624.0 51 135489.5 52 132198.0 53 129219.5 54 121443.0 55 109475.0 56 96958.0 57 91611.0 58 86753.5 59 74183.5 60 60601.0 61 54731.0 62 48079.5 63 35627.5 64 25654.5 65 21482.0 66 17936.5 67 12494.5 68 9182.0 69 6557.0 70 5348.0 71 4446.0 72 3025.0 73 2197.0 74 1888.0 75 1626.0 76 1142.0 77 757.0 78 594.0 79 500.0 80 368.0 81 266.5 82 172.5 83 142.0 84 127.5 85 103.5 86 87.5 87 81.0 88 75.0 89 59.5 90 42.5 91 35.0 92 39.0 93 38.5 94 28.0 95 23.5 96 25.0 97 25.5 98 33.0 99 66.5 100 93.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0031696662935705305 2 0.008419426092296721 3 0.011655127100316636 4 0.018621789474726865 5 0.014263498321067386 6 0.013768237962696989 7 0.016640748041245282 8 0.0189189456897491 9 0.018555754760277477 10-11 0.021444773517438117 12-13 0.020140587907062742 14-15 0.00732985330388185 16-17 0.00628980655130402 18-19 0.01196879199395122 20-21 0.009888698488795561 22-23 0.014032376820494536 24-25 0.02185749048274678 26-27 0.013140908175427823 28-29 0.007973691769763364 30-31 0.007907657055313978 32-33 0.009426455487649858 34-35 0.010829693169699312 36-37 0.009360420773200472 38-39 0.016393117862060085 40-41 0.012166896137299378 42-43 0.015006388858622977 44-45 0.017763338186884844 46-47 0.01673980011291936 48-49 0.014164446249393305 50-51 0.02702470688841124 52-53 0.030342951289492888 54-55 0.0241356881312506 56-57 0.015320053752257562 58-59 0.01602992693258846 60-61 0.019001489082810834 62-63 0.024251248881537023 64-65 0.021114599945191187 66-67 0.019513258119793576 68-69 0.028229840427112532 70-71 0.053851309633474315 72-73 0.06344285190724765 74-75 0.07374426736135187 76-77 0.07222546892901599 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3028710.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.1247768404276 #Duplication Level Percentage of deduplicated Percentage of total 1 72.95438491400182 38.75685418087004 2 14.134904262434667 15.018272692053012 3 5.290755794348906 8.432106626759547 4 2.59092332021745 5.50568892788847 5 1.4254415127650817 3.7863131132363246 6 0.8857277484516929 2.8232453386722347 7 0.548607552245099 2.040125765019588 8 0.38063626178066795 1.617697317157805 9 0.2930944971584149 1.4013521779228317 >10 1.379125801139747 12.909246658697903 >50 0.0793315428870072 2.9157882058248834 >100 0.0341982828779435 3.184214331008982 >500 0.001870767190177376 0.7120207451741988 >1k 9.97742501283698E-4 0.8970739197142145 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4540 0.1498988018001063 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.301735722469302E-5 1.320694288987721E-4 3 0.0 0.0 0.0 1.9810414334815813E-4 1.320694288987721E-4 4 1.650867861234651E-4 0.0 0.0 5.282777155950884E-4 1.650867861234651E-4 5 1.9810414334815813E-4 0.0 0.0 0.0016838852184593441 1.650867861234651E-4 6 2.641388577975442E-4 0.0 0.0 0.0037969960808396973 1.650867861234651E-4 7 2.641388577975442E-4 0.0 0.0 0.008320374020622641 1.9810414334815813E-4 8 2.641388577975442E-4 0.0 0.0 0.013074873460978437 2.3112150057285114E-4 9 2.641388577975442E-4 0.0 0.0 0.017961442330233005 2.3112150057285114E-4 10 2.641388577975442E-4 0.0 0.0 0.0241356881312506 2.971562150222372E-4 11 3.301735722469302E-4 0.0 0.0 0.031762697650154686 3.301735722469302E-4 12 3.6319092947162325E-4 0.0 0.0 0.04242730403373053 3.301735722469302E-4 13 3.6319092947162325E-4 0.0 0.0 0.05586536842418059 3.301735722469302E-4 14 3.6319092947162325E-4 0.0 0.0 0.07220896025040363 3.301735722469302E-4 15 3.6319092947162325E-4 0.0 0.0 0.08954307279336747 3.301735722469302E-4 16 3.6319092947162325E-4 0.0 0.0 0.107339428337477 3.301735722469302E-4 17 3.6319092947162325E-4 0.0 0.0 0.12516880123881124 3.301735722469302E-4 18 3.9620828669631626E-4 0.0 0.0 0.1438566254279875 3.301735722469302E-4 19 5.282777155950884E-4 0.0 0.0 0.16343591826223045 3.301735722469302E-4 20 6.273297872691674E-4 0.0 0.0 0.18605280796114518 3.301735722469302E-4 21 6.273297872691674E-4 0.0 0.0 0.20942909687622782 3.301735722469302E-4 22 8.914686450667116E-4 0.0 0.0 0.23726272901664405 3.301735722469302E-4 23 9.575033595160976E-4 0.0 0.0 0.2649642917281615 3.6319092947162325E-4 24 9.905207167407906E-4 0.0 0.0 0.29253378501078015 4.2922564392100927E-4 25 9.905207167407906E-4 0.0 0.0 0.3217541461546335 4.2922564392100927E-4 26 9.905207167407906E-4 0.0 0.0 0.3543752950926302 4.2922564392100927E-4 27 9.905207167407906E-4 0.0 0.0 0.3836947083081576 4.2922564392100927E-4 28 9.905207167407906E-4 0.0 0.0 0.41552344067276165 4.952603583703953E-4 29 9.905207167407906E-4 0.0 0.0 0.450554856688161 5.282777155950884E-4 30 9.905207167407906E-4 0.0 0.0 0.4876003314942665 5.282777155950884E-4 31 9.905207167407906E-4 0.0 0.0 0.5281126288089649 5.282777155950884E-4 32 0.0010235380739654837 0.0 0.0 0.5712002799871893 5.282777155950884E-4 33 0.0010235380739654837 0.0 0.0 0.6124719765180555 5.943124300444744E-4 34 0.0010235380739654837 0.0 0.0 0.659521710563243 5.943124300444744E-4 35 0.0010235380739654837 0.0 0.0 0.7066704966801047 5.943124300444744E-4 36 0.0010235380739654837 0.0 0.0 0.7561965325171442 5.943124300444744E-4 37 0.0010235380739654837 0.0 0.0 0.8086941305044062 5.943124300444744E-4 38 0.0010235380739654837 0.0 0.0 0.8617200062072632 6.933645017185534E-4 39 0.0010235380739654837 0.0 0.0 0.9198965896371722 6.933645017185534E-4 40 0.0010565554311901767 0.0 0.0 0.9810777525745284 7.593992161679395E-4 41 0.0010565554311901767 0.0 0.0 1.0440748701592426 7.593992161679395E-4 42 0.0011225901456395628 0.0 0.0 1.114798049334535 7.593992161679395E-4 43 0.0011225901456395628 0.0 0.0 1.193445394243754 8.914686450667116E-4 44 0.0011886248600889487 0.0 0.0 1.2721257565101973 8.914686450667116E-4 45 0.0011886248600889487 0.0 0.0 1.3522918998517521 8.914686450667116E-4 46 0.0011886248600889487 0.0 0.0 1.439523757639391 8.914686450667116E-4 47 0.0012216422173136417 0.0 0.0 1.5290007957183092 9.905207167407906E-4 48 0.0012216422173136417 0.0 0.0 1.6225059513786397 9.905207167407906E-4 49 0.0012876769317630278 0.0 0.0 1.7168365409695878 0.0011556075028642558 50 0.0012876769317630278 0.0 0.0 1.8184969838644176 0.0011556075028642558 51 0.0012876769317630278 0.0 0.0 1.9291381479243639 0.0012216422173136417 52 0.0012876769317630278 0.0 0.0 2.043873464280172 0.0012216422173136417 53 0.0012876769317630278 0.0 0.0 2.1703629598079712 0.0012216422173136417 54 0.0012876769317630278 0.0 0.0 2.30655955835983 0.0012876769317630278 55 0.0012876769317630278 0.0 0.0 2.4416665841232734 0.001353711646212414 56 0.001353711646212414 0.0 0.0 2.58776838984254 0.001353711646212414 57 0.001353711646212414 0.0 0.0 2.7329457095595155 0.001386729003437107 58 0.001386729003437107 0.0 0.0 2.883207702289093 0.001518798432335879 59 0.001386729003437107 0.0 0.0 3.036804448098365 0.001518798432335879 60 0.001386729003437107 0.0 0.0 3.1978300992831934 0.001518798432335879 61 0.001452763717886493 0.0 0.0 3.3710721726411577 0.001518798432335879 62 0.001452763717886493 0.0 0.0 3.550521509157364 0.001518798432335879 63 0.001518798432335879 0.0 0.0 3.73862139326644 0.001650867861234651 64 0.001518798432335879 0.0 0.0 3.931706898316445 0.001650867861234651 65 0.001518798432335879 0.0 0.0 4.135985287465621 0.001650867861234651 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGGCG 1220 0.0 16.00261 17 GCGTATA 245 2.1130836E-7 15.938346 43 TAACCGG 280 5.7771103E-8 15.215886 59 GTAACGT 295 1.0993426E-7 14.4374275 2 GGTAACG 275 7.8371704E-7 14.196804 1 CGAACTC 935 0.0 14.046822 25 GGCGGAA 1410 0.0 13.847374 20 TAACGTT 335 3.4708137E-8 13.773019 3 TCGAACT 940 0.0 13.595378 24 GACGCGA 680 0.0 13.58079 69 GTGCGAC 185 0.0013876073 13.438284 67 CTAACCG 325 3.5694575E-7 13.108205 58 GCCTCGA 955 0.0 13.0105505 21 ATCGTAC 220 3.4952935E-4 12.906185 33 CGCGACC 750 0.0 12.786835 71 CTCGAAC 1030 0.0 12.752295 23 AGGCGGA 1560 0.0 12.743457 19 GTAGACC 1615 0.0 12.527212 8 TCGCGCT 200 0.0025041166 12.423844 37 GCGGAAG 1610 0.0 12.3479 21 >>END_MODULE