FastQCFastQC Report
Thu 26 May 2016
SRR936014_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936014_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028710
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54840.18106718702021654No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38120.12586216574052977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12200.038.539986100-101
GGTATCA62350.034.0730861
GTATCAA119700.030.574591
CGGTATC11450.030.408474100-101
CCTGTCG8150.027.01728896-97
GCGTATC3500.026.357723100-101
CGTACAT17500.025.847574100-101
TCGGTAT4000.025.294914100-101
GCGTACA5900.025.219255100-101
TAAATCG3800.025.05728796-97
GGGTATC12900.024.222212100-101
ATCAACG151200.024.04153
TCAACGC152500.023.7975434
TATCAAC154050.023.673962
AGGTATC19750.023.204268100-101
AGTATCA29200.023.134348100-101
CAACGCA157300.023.0713635
CGAGTAC11400.022.710602100-101
CCGTACA6700.022.652159100-101
TCGTATC7800.021.746758100-101
Produced by FastQC (version 0.11.5)