FastQCFastQC Report
Thu 26 May 2016
SRR936013_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936013_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2672405
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.20771552216075034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTAC2650.026.7858094
ATTCGCG1904.7293724E-1022.42052756
CGATCGT2301.0913936E-1121.604551
CTCGCGC2051.2223609E-920.77805536
TTAGTCG1205.1325966E-520.70668615
CGCTCCG2302.3646862E-1020.06399525
CGGCGTA900.004858914319.72508246
CCTCGCG2253.8944563E-918.93111835
ACGATCG1902.5944792E-718.68377159
CAATTCG2507.185008E-1018.46094954
TTGCGTA2507.185008E-1018.4578436
CGCGCTT2356.681148E-918.12452138
AATTCGC2559.349606E-1018.09761455
TCGCGCT2408.6602086E-917.74891937
TCGAACT6500.017.47716724
CGAACTC6500.017.47585925
CGTAAGT2852.2009772E-1017.43590439
CGCGGAA2258.008283E-817.35514859
CTCGAAC6350.017.331623
TGCGTAA2752.557499E-916.78079637
Produced by FastQC (version 0.11.5)