##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936013_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2672405 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.95317962659103 40.0 37.0 41.0 31.0 41.0 2 36.822357015497275 40.0 36.0 41.0 30.0 41.0 3 36.73755811712671 40.0 36.0 41.0 30.0 41.0 4 36.62066153895087 39.0 36.0 41.0 30.0 41.0 5 36.55036081731624 39.0 36.0 41.0 30.0 41.0 6 36.45534153693022 39.0 36.0 41.0 30.0 41.0 7 36.29739728821043 39.0 36.0 41.0 30.0 41.0 8 36.11830953766364 39.0 35.0 40.0 29.0 41.0 9 36.032628288002755 39.0 35.0 40.0 29.0 41.0 10-11 36.24048544288759 39.0 35.0 41.0 30.0 41.0 12-13 36.42795440810805 39.5 36.0 41.0 30.0 41.0 14-15 36.37749723563606 40.0 36.0 41.0 30.0 41.0 16-17 36.22602805338263 40.0 35.0 41.0 29.5 41.0 18-19 36.08259021368393 39.5 35.0 41.0 29.0 41.0 20-21 35.89062417560213 39.0 35.0 41.0 28.0 41.0 22-23 35.642609933748815 39.0 35.0 41.0 27.0 41.0 24-25 35.4168312811868 39.0 35.0 41.0 26.5 41.0 26-27 34.49200308336498 38.0 34.0 40.0 25.0 40.5 28-29 34.5409183862476 38.0 34.0 40.0 25.0 40.5 30-31 34.92648026777378 39.0 34.0 40.0 26.0 41.0 32-33 34.78477794346291 38.5 34.0 40.0 25.0 41.0 34-35 34.53006973119718 38.0 34.0 40.0 24.0 41.0 36-37 34.25588823550322 38.0 34.0 40.0 23.0 41.0 38-39 33.987351655157056 37.5 33.5 40.0 23.0 41.0 40-41 33.62207618231518 37.0 33.0 40.0 22.0 41.0 42-43 33.271869720345535 36.5 33.0 39.5 21.5 41.0 44-45 32.90658377004982 36.0 32.5 39.0 20.0 41.0 46-47 32.519880220251046 35.5 32.0 39.0 18.5 40.5 48-49 32.05860021216844 35.0 32.0 38.5 17.5 40.0 50-51 31.617344863521808 35.0 31.0 37.0 13.5 39.5 52-53 31.157501389198117 35.0 31.0 37.0 8.0 39.0 54-55 30.690446994373982 35.0 31.0 36.0 7.0 39.0 56-57 30.23036702894958 34.5 30.0 36.0 2.0 37.5 58-59 29.792633414471233 34.0 30.0 35.5 2.0 37.0 60-61 29.43530097421611 34.0 29.5 35.0 2.0 37.0 62-63 29.086353864777234 34.0 29.0 35.0 2.0 36.0 64-65 28.70638525971924 34.0 29.0 35.0 2.0 36.0 66-67 28.326155092510305 34.0 29.0 35.0 2.0 35.5 68-69 27.96819119856459 34.0 28.5 35.0 2.0 35.0 70-71 27.547370439734994 34.0 27.0 35.0 2.0 35.0 72-73 27.0742724250254 33.5 26.5 35.0 2.0 35.0 74-75 26.497447056116123 33.0 25.0 35.0 2.0 35.0 76-77 24.787937082889755 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.805436781060962 1101 2 0.7621062994555672 1101 3 0.8130592573615232 1101 4 0.8982399091839568 1101 5 0.9591050750141505 1101 6 0.8741512542919736 1101 7 1.0742463962401345 1101 8 1.0556521732820343 1101 9 1.0439578613459233 1101 10-11 1.0713740049648663 1101 12-13 1.013739636116803 1101 14-15 0.9815406472989565 1101 16-17 1.105198179597373 1101 18-19 1.0715205828199785 1101 20-21 1.121383596939566 1101 22-23 1.07417216956555 1101 24-25 1.0936119788376928 1101 26-27 1.0901908676844698 1101 28-29 1.1555262043186545 1101 30-31 1.2351144348432896 1101 32-33 1.299649705869399 1101 34-35 1.1197579967508844 1101 36-37 1.2175265059437592 1101 38-39 1.2410658762238143 1101 40-41 1.2749647861487219 1101 42-43 1.3621060250120536 1101 44-45 1.0959553360469343 1101 46-47 1.079455975125093 1101 48-49 1.2920933953627554 1101 50-51 1.2137237911821437 1101 52-53 1.2318393556558505 1101 54-55 1.202471811020807 1101 56-57 1.28002805524358 1101 58-59 1.341563316303592 1101 60-61 1.183192273371116 1101 62-63 1.0901052342798536 1101 64-65 0.9110522815262279 1101 66-67 0.8723282656510492 1101 68-69 1.0308067900793247 1101 70-71 1.1238832823731784 1101 72-73 0.9593597822659667 1101 74-75 0.9512849043916063 1101 76-77 0.9371979841846816 1102 1 0.9355721443499618 1102 2 1.059408783584196 1102 3 1.0505725863505972 1102 4 1.0441244603919628 1102 5 0.9536488286549343 1102 6 0.9756996385223005 1102 7 0.7839724855147381 1102 8 1.013498628264614 1102 9 0.8860710491464445 1102 10-11 0.8902746965912911 1102 12-13 1.0435509075049012 1102 14-15 0.9465532851552609 1102 16-17 1.0092208191321461 1102 18-19 1.1344654995384786 1102 20-21 1.1448003475294968 1102 22-23 1.1260200858002563 1102 24-25 1.2148844900192302 1102 26-27 1.2035332474520501 1102 28-29 1.1401377013122271 1102 30-31 1.1577581657754337 1102 32-33 1.0757492240511226 1102 34-35 1.2159226418542062 1102 36-37 1.2196751883485035 1102 38-39 1.2980556351172154 1102 40-41 1.2604741386560505 1102 42-43 1.3145789227342775 1102 44-45 1.2243215228699995 1102 46-47 1.0344240472595985 1102 48-49 1.1446605833409933 1102 50-51 1.076486421633458 1102 52-53 0.9356781662163556 1102 54-55 0.9599684417485648 1102 56-57 1.1556321793315334 1102 58-59 1.1642985606190273 1102 60-61 1.0809876215539305 1102 62-63 1.174921109105835 1102 64-65 1.0948411454026932 1102 66-67 1.0053392349153825 1102 68-69 1.0035243241176133 1102 70-71 0.9604556834671385 1102 72-73 1.0728234188091186 1102 74-75 1.010567743071345 1102 76-77 1.1657540473937082 1103 1 0.4873237426046586 1103 2 0.5200899586254977 1103 3 0.5568984710392044 1103 4 0.5521592553042112 1103 5 0.5337143515416471 1103 6 0.5060062885952661 1103 7 0.6994469430711732 1103 8 0.7302459484563286 1103 9 0.5092440754963476 1103 10-11 0.598786977039623 1103 12-13 0.6929543862553302 1103 14-15 0.6870267241252748 1103 16-17 0.6347754857395458 1103 18-19 0.7527761646939339 1103 20-21 0.7735304573145925 1103 22-23 0.8006053359280472 1103 24-25 0.5991103365571533 1103 26-27 0.4816654470060939 1103 28-29 0.4997401470308276 1103 30-31 0.8219524453940821 1103 32-33 0.6866144854389233 1103 34-35 0.673413557915282 1103 36-37 0.6388031302112935 1103 38-39 0.6223950818210682 1103 40-41 0.6062992769986124 1103 42-43 0.5720187705759869 1103 44-45 0.6446463037813146 1103 46-47 0.29740245878333127 1103 48-49 0.37939521379784225 1103 50-51 0.38896604990496897 1103 52-53 0.38354390356181156 1103 54-55 0.4690178876375555 1103 56-57 0.581562889527028 1103 58-59 0.42563658500583657 1103 60-61 0.4036959409974479 1103 62-63 0.4278331087947045 1103 64-65 0.34612873124174826 1103 66-67 0.42286614411673895 1103 68-69 0.4577888676797741 1103 70-71 0.30946830282695004 1103 72-73 0.24775313634584606 1103 74-75 0.24435670863201153 1103 76-77 0.3666195217586683 1104 1 0.43655286657136827 1104 2 0.34723764267734936 1104 3 0.1975610738427278 1104 4 0.3214450150611228 1104 5 0.42927775947254787 1104 6 0.05567058279678605 1104 7 0.4161495058757083 1104 8 0.48012449249557676 1104 9 0.3486438104406062 1104 10-11 0.2144596542153181 1104 12-13 0.3627314677363742 1104 14-15 0.4240576642507534 1104 16-17 0.4828157271833362 1104 18-19 0.3716606095891706 1104 20-21 0.3930897252344181 1104 22-23 0.4487810750123131 1104 24-25 0.43723901012403843 1104 26-27 0.1919858227017457 1104 28-29 0.26146593952526587 1104 30-31 0.46812747356120354 1104 32-33 0.47479369077731803 1104 34-35 0.4766930516106882 1104 36-37 0.4773221381072261 1104 38-39 0.5194349221175258 1104 40-41 0.47171868613325785 1104 42-43 0.34506456320443846 1104 44-45 0.3451885227870548 1104 46-47 0.33632532488206124 1104 48-49 0.49371017324297384 1104 50-51 0.31137274812125426 1104 52-53 0.4353907499558858 1104 54-55 0.4366511305375944 1104 56-57 0.42169729977495507 1104 58-59 0.31206817994267055 1104 60-61 0.16954400210342868 1104 62-63 0.29208859670043097 1104 64-65 0.22847217184315838 1104 66-67 0.16786771733313088 1104 68-69 0.134103706506572 1104 70-71 0.27378575992444 1104 72-73 0.14258856999159875 1104 74-75 -0.029365429979776536 1104 76-77 -0.030451234600523236 1105 1 0.1975050486678427 1105 2 0.19860806418073196 1105 3 0.24143430182871128 1105 4 0.1243660369507893 1105 5 0.1991926860000035 1105 6 -0.01398538533621263 1105 7 0.04421541186708566 1105 8 0.2748195653861245 1105 9 -0.0036800051517360544 1105 10-11 0.13568494261852493 1105 12-13 0.3374137247063729 1105 14-15 0.11991054996286721 1105 16-17 0.1642975019963373 1105 18-19 0.3710168135807095 1105 20-21 0.3046063238315 1105 22-23 0.39433441258162105 1105 24-25 0.32092673126016535 1105 26-27 0.23896943140336901 1105 28-29 0.24617173434933903 1105 30-31 0.20816018535838765 1105 32-33 0.50062683061185 1105 34-35 0.5715727133567867 1105 36-37 0.5548508074294816 1105 38-39 0.4600030405951827 1105 40-41 0.5098680996068907 1105 42-43 0.36844206826391 1105 44-45 0.21811156066421233 1105 46-47 0.17954554691667823 1105 48-49 0.07666655891338081 1105 50-51 0.23460652407183957 1105 52-53 0.1841300077724881 1105 54-55 0.24870979577386976 1105 56-57 0.3873944624525478 1105 58-59 0.3838620848204606 1105 60-61 0.016654000021439685 1105 62-63 0.16325679545759897 1105 64-65 0.31329154596303965 1105 66-67 0.3849504214203705 1105 68-69 0.2367668199952675 1105 70-71 -0.21042908794350268 1105 72-73 -0.19084871269724957 1105 74-75 -0.3631960102301761 1105 76-77 -0.24358041319961643 1106 1 0.19810059306885108 1106 2 0.2143022691334764 1106 3 -0.1423051392202126 1106 4 0.23959530198460044 1106 5 -0.060605509009938885 1106 6 0.23081857252127236 1106 7 0.4744458354890426 1106 8 0.4991589444430602 1106 9 0.2848731439510317 1106 10-11 0.12835514222670952 1106 12-13 0.2502690877657727 1106 14-15 0.2518881846976342 1106 16-17 0.4492313420991323 1106 18-19 0.553825923393795 1106 20-21 0.3183107664904128 1106 22-23 0.36718538203400897 1106 24-25 0.5075828051209115 1106 26-27 0.4591065962027514 1106 28-29 0.5805518595972288 1106 30-31 0.5143435853481506 1106 32-33 0.34199038353525424 1106 34-35 0.3909503258225939 1106 36-37 0.5347193603378386 1106 38-39 0.5286531499839668 1106 40-41 0.4438376031593947 1106 42-43 0.48535474682942237 1106 44-45 0.3370644076312743 1106 46-47 0.5405792181966333 1106 48-49 0.3742451096851198 1106 50-51 0.3358931587795304 1106 52-53 0.45377996368594964 1106 54-55 0.4677858743893424 1106 56-57 0.38345104970326105 1106 58-59 0.30130590834190585 1106 60-61 0.333502580073862 1106 62-63 0.44292868931286833 1106 64-65 0.3594671518143606 1106 66-67 0.30371368123601883 1106 68-69 0.17982868258370388 1106 70-71 0.1168517508141278 1106 72-73 0.26662312759212625 1106 74-75 0.0857263523266596 1106 76-77 0.11824606339840571 1107 1 0.38156962106629067 1107 2 0.3009420209949525 1107 3 0.2861372419507262 1107 4 0.31908382323921103 1107 5 0.2857766325003439 1107 6 0.244487824451447 1107 7 0.04044460181260234 1107 8 0.23345105680701295 1107 9 0.13953465763110273 1107 10-11 0.22799468652651456 1107 12-13 0.3351384262287027 1107 14-15 0.2809297174967682 1107 16-17 0.21864391332056243 1107 18-19 0.40923348974775564 1107 20-21 0.27350302414754424 1107 22-23 0.3428869258836116 1107 24-25 0.10281853379395756 1107 26-27 0.07844184339116822 1107 28-29 -0.004606018830095593 1107 30-31 0.2444641465247699 1107 32-33 0.27285802057711805 1107 34-35 0.3070902925542569 1107 36-37 0.3303972538499167 1107 38-39 0.34512843114233505 1107 40-41 0.1488866140256846 1107 42-43 0.17151813002628558 1107 44-45 0.26338132800067626 1107 46-47 0.19910094054927896 1107 48-49 0.3398840049892584 1107 50-51 0.16996280010710407 1107 52-53 0.19009287899201865 1107 54-55 0.18113983720949278 1107 56-57 0.16618454928082826 1107 58-59 0.1186684425822726 1107 60-61 0.19596315066383596 1107 62-63 0.20599922812544236 1107 64-65 0.14980968704868403 1107 66-67 0.22635632142054263 1107 68-69 0.11151224165254447 1107 70-71 0.10900898901600087 1107 72-73 0.09278671542725547 1107 74-75 0.1114115251144483 1107 76-77 0.29013607325027735 1108 1 -0.18508289569754055 1108 2 -0.12800609042934497 1108 3 -0.18865514716559773 1108 4 -0.3608239809482825 1108 5 -0.15933220680695825 1108 6 -0.1927305204659433 1108 7 -0.22574645734422205 1108 8 -0.2560869760768725 1108 9 -0.2899359117439104 1108 10-11 -0.3607435015183711 1108 12-13 -0.3209011285673071 1108 14-15 -0.12454311398605 1108 16-17 -0.2681031497535713 1108 18-19 -0.15519879184807195 1108 20-21 -0.29119956469352815 1108 22-23 -0.2209789138957703 1108 24-25 -0.10497264854584643 1108 26-27 -0.22186465026009472 1108 28-29 -0.18533642686806928 1108 30-31 -0.06625160397791774 1108 32-33 -0.20691249764993103 1108 34-35 -0.12394617281502462 1108 36-37 -0.20921721947389216 1108 38-39 -0.3914571231768207 1108 40-41 -0.27935620731480526 1108 42-43 -0.12660859361344734 1108 44-45 -0.23953483748450566 1108 46-47 -0.19902771134312758 1108 48-49 -0.2784519467329254 1108 50-51 -0.3143339042135018 1108 52-53 -0.39905967225517003 1108 54-55 -0.2302879170742571 1108 56-57 -0.21814203591880243 1108 58-59 -0.20200673298020533 1108 60-61 -0.25568513942643634 1108 62-63 -0.2998687121826116 1108 64-65 -0.3455628584052981 1108 66-67 -0.4415035630137858 1108 68-69 -0.5082810890703762 1108 70-71 -0.5615384855500629 1108 72-73 -0.4016677987811512 1108 74-75 -0.5070965618646639 1108 76-77 -0.6179295543368504 1109 1 0.07773488996318889 1109 2 -0.06502859318015908 1109 3 -0.2967917094575583 1109 4 0.012259243793110386 1109 5 -0.009563038419813097 1109 6 0.01171610623663355 1109 7 -0.06352229509326435 1109 8 -0.06694761791553105 1109 9 0.026586904972873526 1109 10-11 -0.1484120493485861 1109 12-13 0.004467307284286903 1109 14-15 -0.01908453401469501 1109 16-17 0.03487809562736288 1109 18-19 -0.0710155129998995 1109 20-21 -0.047215506521624206 1109 22-23 0.06605965731302632 1109 24-25 -0.1009572967171195 1109 26-27 -0.15043658245021163 1109 28-29 -0.08354320387946501 1109 30-31 0.0803425104399409 1109 32-33 0.05865497556072796 1109 34-35 0.12633765157103483 1109 36-37 0.1465516248648342 1109 38-39 0.10735942325960934 1109 40-41 0.1511125643880149 1109 42-43 0.1428338527518207 1109 44-45 0.14046699004700258 1109 46-47 -0.10268803415344507 1109 48-49 0.038991122346772045 1109 50-51 0.10616579592185715 1109 52-53 0.21521470255208186 1109 54-55 0.28308098355696387 1109 56-57 0.27927333252127795 1109 58-59 0.1992324631146296 1109 60-61 0.1034445438169449 1109 62-63 0.13638837073584753 1109 64-65 0.2530811865423921 1109 66-67 0.18791824183433548 1109 68-69 0.18679274052330896 1109 70-71 0.14000291600584802 1109 72-73 0.18908197281266226 1109 74-75 0.07116691092746663 1109 76-77 0.2632064426330558 1110 1 -0.4447926990068183 1110 2 -0.4459217678525462 1110 3 -0.46964292561359855 1110 4 -0.3344067571582485 1110 5 -0.2981915988547783 1110 6 -0.5680472156899938 1110 7 -0.5000827919267294 1110 8 -0.24500321726417695 1110 9 -0.3770879792075874 1110 10-11 -0.37185231501240423 1110 12-13 -0.4271229825705092 1110 14-15 -0.47170611366486526 1110 16-17 -0.5744514657413191 1110 18-19 -0.4188184761023592 1110 20-21 -0.6749682641975454 1110 22-23 -0.8098985182181906 1110 24-25 -0.6754852335595487 1110 26-27 -0.6096578339280612 1110 28-29 -0.4667225815025873 1110 30-31 -0.46851545313874254 1110 32-33 -0.2949574842419622 1110 34-35 -0.23940618081631726 1110 36-37 -0.34811625329515294 1110 38-39 -0.3160646155935609 1110 40-41 -0.1875162131280348 1110 42-43 -0.26168432358259963 1110 44-45 -0.3813670248756935 1110 46-47 -0.5253712838246329 1110 48-49 -0.39350405641813424 1110 50-51 -0.46735027174122834 1110 52-53 -0.44010558836912494 1110 54-55 -0.4616542575753435 1110 56-57 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-0.668056702806652 2314 10-11 -0.5294142065468606 2314 12-13 -0.7520087535994762 2314 14-15 -0.7321492734173489 2314 16-17 -1.0322044392331193 2314 18-19 -0.9285517133049481 2314 20-21 -0.7636378118375475 2314 22-23 -0.6272084646533216 2314 24-25 -0.6205856621669952 2314 26-27 -0.6080909176458391 2314 28-29 -0.586769157729691 2314 30-31 -0.7984182313465524 2314 32-33 -0.8361307805384257 2314 34-35 -0.8761193499902049 2314 36-37 -1.0170125780637065 2314 38-39 -0.888340702482779 2314 40-41 -0.644961774674556 2314 42-43 -0.49505311618347747 2314 44-45 -0.5752510752824094 2314 46-47 -0.6122527555095623 2314 48-49 -0.5648309259914299 2314 50-51 -0.539836460319659 2314 52-53 -0.4066573994456846 2314 54-55 -0.5449219802057286 2314 56-57 -0.4063017423171331 2314 58-59 -0.34519930757559436 2314 60-61 -0.33083418865802017 2314 62-63 -0.3825780455843386 2314 64-65 -0.3846225220133306 2314 66-67 -0.3195564848215078 2314 68-69 -0.2531196080301541 2314 70-71 -0.15851608978471887 2314 72-73 -0.19217143825482808 2314 74-75 -0.14903252692010582 2314 76-77 -0.23003619544628506 2315 1 -1.1345967411335494 2315 2 -0.9315798669282032 2315 3 -0.8506633265568553 2315 4 -0.7514243376158944 2315 5 -0.7801977058404788 2315 6 -0.8772031804924794 2315 7 -0.8580776852862684 2315 8 -0.9465375272760497 2315 9 -0.9963747574493453 2315 10-11 -0.997181605487043 2315 12-13 -0.8653491020727273 2315 14-15 -1.2422584506299401 2315 16-17 -0.9689789063092249 2315 18-19 -0.8466833367798827 2315 20-21 -0.9279726547372036 2315 22-23 -1.0367201530019017 2315 24-25 -1.2513142696781117 2315 26-27 -1.041924762636377 2315 28-29 -1.0252369834242288 2315 30-31 -1.2059346478120219 2315 32-33 -1.2460059558486307 2315 34-35 -1.1636297238195752 2315 36-37 -1.3855414256412146 2315 38-39 -1.2221872774390476 2315 40-41 -1.2989580278030957 2315 42-43 -1.3983472816522742 2315 44-45 -1.377299889510681 2315 46-47 -1.1656016926120571 2315 48-49 -1.1737599862085375 2315 50-51 -1.10986585943796 2315 52-53 -1.231725379101782 2315 54-55 -1.1120435382661178 2315 56-57 -1.1342710068256068 2315 58-59 -1.0707170429310793 2315 60-61 -1.2621534010173399 2315 62-63 -1.2599823563987478 2315 64-65 -1.2149595601491363 2315 66-67 -1.2007784741163157 2315 68-69 -1.0807005266310412 2315 70-71 -1.1521959622883742 2315 72-73 -2.4725908933576584 2315 74-75 -3.197524353790385 2315 76-77 -2.8793228529670465 2316 1 -3.2783059037148305 2316 2 -3.1010449904897683 2316 3 -2.7648581041039364 2316 4 -2.555407753972702 2316 5 -3.183863764633209 2316 6 -2.8630316916223606 2316 7 -3.2718885285995256 2316 8 -3.114475466241295 2316 9 -2.6387854118217007 2316 10-11 -2.599776911437104 2316 12-13 -2.81306411445388 2316 14-15 -2.755999362125074 2316 16-17 -3.9741181383881283 2316 18-19 -3.719416901157949 2316 20-21 -3.0947646460638225 2316 22-23 -3.1343840487107357 2316 24-25 -3.1787844483298997 2316 26-27 -2.839652866674925 2316 28-29 -2.8491821510895434 2316 30-31 -3.100901859460045 2316 32-33 -3.2224665637751997 2316 34-35 -3.1723715300209463 2316 36-37 -3.0949919575967044 2316 38-39 -3.133503206104649 2316 40-41 -2.9879880741436153 2316 42-43 -3.2697160243005534 2316 44-45 -2.9693173994379762 2316 46-47 -2.7810155074026 2316 48-49 -2.901757909963777 2316 50-51 -3.16641181970774 2316 52-53 -4.195802792242908 2316 54-55 -3.3064428746046133 2316 56-57 -3.165042454118197 2316 58-59 -3.224817231608835 2316 60-61 -3.12646876654431 2316 62-63 -3.2577289466469743 2316 64-65 -3.08947385308122 2316 66-67 -3.1619949982878595 2316 68-69 -3.198415775846197 2316 70-71 -2.9783488833726537 2316 72-73 -2.94709781812076 2316 74-75 -2.8396922533096145 2316 76-77 -2.9087117159796314 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57769.0 3 4977.0 4 6418.0 5 7721.0 6 8921.0 7 9385.0 8 11131.0 9 11629.0 10 14643.0 11 16686.0 12 12934.0 13 10916.0 14 10741.0 15 11133.0 16 11167.0 17 11965.0 18 12648.0 19 13547.0 20 14635.0 21 15815.0 22 17583.0 23 20230.0 24 23642.0 25 27526.0 26 32635.0 27 39013.0 28 46066.0 29 55955.0 30 66953.0 31 81565.0 32 101152.0 33 128670.0 34 171683.0 35 231260.0 36 330901.0 37 458056.0 38 436870.0 39 126700.0 40 1164.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.33442147858769 26.991116827730377 28.986116324386792 21.688345369295142 2 21.950122340158785 27.164904354621044 28.84948605157523 22.03548725364494 3 22.29091310555438 27.345787012520418 28.351088215536098 22.0122116663891 4 21.807274817628063 27.52371621085534 28.708366579192006 21.960642392324587 5 22.223818976021747 27.279677914799212 28.617208714683052 21.87929439449599 6 22.26657665216405 27.18454195516589 28.703499263891935 21.84538212877812 7 22.15583384200597 26.88411283438131 28.73434638716431 22.225706936448418 8 21.954020966654777 27.349017966980753 28.4677111196026 22.22924994676187 9 22.04464581109626 26.996396658038808 28.74443667654205 22.21452085432289 10-11 22.485597203763497 27.19657379437751 28.389854772506368 21.927974229352625 12-13 22.474368827284376 26.988381882345763 28.33872350968314 22.19852578068672 14-15 22.183102939971235 27.172626431936003 28.552535412243763 22.091735215849 16-17 22.386353090160107 27.029676404373287 28.594590124181206 21.9893803812854 18-19 22.276492444096796 27.047242289479374 28.357743081513558 22.318522184910275 20-21 22.314061186496122 27.301249435766454 28.26505015783568 22.119639219901742 22-23 22.365142712352483 27.30938906215672 27.951941786998713 22.373526438492082 24-25 22.385079782301833 27.444468085862887 27.798527956788227 22.37192417504705 26-27 22.395207474070773 27.45927893152917 27.788408580389547 22.357105014010514 28-29 22.550216948293723 27.333557744321542 27.79074991378764 22.325475393597102 30-31 22.394142872272386 27.250826390455973 27.891599427130753 22.463431310140887 32-33 22.351084983869647 27.66336974177779 27.72098281655896 22.264562457793605 34-35 22.386888104854744 27.288486205957724 27.820618975310406 22.50400671387712 36-37 22.39683753877106 27.315897333029955 28.019408733817947 22.26785639438104 38-39 22.337254992953888 27.3107948775477 28.088234441057313 22.2637156884411 40-41 22.16040243942601 27.348673554622128 27.945862137102452 22.545061868849416 42-43 22.141780900869612 27.567144561593654 27.728353635259932 22.5627209022768 44-45 22.21599309440054 27.242406488389275 28.014776755315097 22.526823661895083 46-47 22.132077276043965 27.258592834312683 28.00291869007782 22.606411199565528 48-49 22.27849157787416 27.20771083760141 27.836750291566798 22.677047292957635 50-51 22.18648620978599 27.4000198020432 27.740248102007847 22.673245886162956 52-53 22.171180413421805 27.33422689253837 27.79886270539555 22.695729988644274 54-55 22.18151288875547 27.294326110395055 27.80994370333994 22.71421729750954 56-57 22.068782768770586 27.442819039192557 27.783351326507717 22.705046865529138 58-59 22.18955799815754 27.39983648759332 27.784103742240973 22.626501772008165 60-61 22.15480549849992 27.284685252498637 27.727261499122708 22.833247749878733 62-63 22.222600674064317 27.131042048640502 27.78498811713201 22.86136916016317 64-65 22.234548943978606 27.151806759921808 27.69436342556853 22.919280870531054 66-67 22.092463112821243 27.269319449331125 27.8615168097737 22.776700628073932 68-69 22.263773341152323 27.034567207525846 27.879126611660464 22.822532839661367 70-71 22.062434246734334 26.928232065504293 27.937378555356297 23.07195513240508 72-73 22.20481995393517 26.78901934348258 27.853678632286577 23.152482070295676 74-75 22.103223252524348 26.80338086571719 27.705335797369766 23.38806008438869 76-77 22.00894799202018 26.69809506384775 28.06121159130325 23.231745352828828 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5207.0 1 3220.0 2 1213.5 3 1266.5 4 1339.0 5 1418.5 6 1540.5 7 1664.5 8 1797.0 9 1848.0 10 1933.5 11 2130.0 12 2301.0 13 2361.0 14 2513.5 15 2802.0 16 3240.0 17 3542.0 18 3898.0 19 4742.0 20 6016.0 21 7871.0 22 8940.0 23 10489.0 24 14048.0 25 19318.0 26 22578.0 27 25782.0 28 33369.0 29 41161.5 30 44571.0 31 50532.0 32 61434.0 33 71012.5 34 80150.5 35 84651.0 36 89622.5 37 99161.0 38 105345.5 39 106963.0 40 110154.0 41 116944.5 42 125701.0 43 130858.0 44 129368.5 45 128523.5 46 129261.5 47 130439.0 48 131523.0 49 130048.0 50 125466.0 51 118123.5 52 113888.0 53 110843.0 54 102822.5 55 90368.5 56 76908.0 57 70926.0 58 66817.0 59 56063.5 60 44939.5 61 40460.0 62 35301.5 63 25539.5 64 18038.5 65 15141.0 66 13031.5 67 9737.0 68 7221.5 69 4932.0 70 3973.0 71 3324.0 72 2289.0 73 1772.5 74 1642.0 75 1443.5 76 1006.0 77 627.0 78 487.0 79 392.0 80 259.5 81 204.0 82 145.0 83 104.0 84 101.0 85 79.0 86 57.5 87 55.0 88 51.0 89 44.0 90 39.0 91 37.0 92 37.5 93 43.5 94 40.0 95 33.5 96 36.0 97 39.5 98 41.0 99 83.0 100 127.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.010252936961276453 2 0.012984558852419451 3 0.0138452068455193 4 0.014705854838619145 5 0.01186197451359356 6 0.0090929331444897 7 0.015641341787640723 8 0.01676392612646661 9 0.016501989780740568 10-11 0.01975748436333565 12-13 0.0155103736147777 14-15 0.00883099679876366 16-17 0.006772925510916197 18-19 0.018241995505920698 20-21 0.023911046416991436 22-23 0.021029746614004988 24-25 0.020225227837846433 26-27 0.024752984671110853 28-29 0.008400672802213736 30-31 0.009055513666528838 32-33 0.010047129832491706 34-35 0.012254879032182623 36-37 0.011562618689906656 38-39 0.013639399716734554 40-41 0.011263262866219753 42-43 0.015304566485992955 44-45 0.013863916584499729 46-47 0.024640726237228263 48-49 0.02368652954922626 50-51 0.036053667015291474 52-53 0.039870453767299495 54-55 0.028232996121471107 56-57 0.02089877844114197 58-59 0.016333602129916688 60-61 0.02054329340051377 62-63 0.026249763789545372 64-65 0.026287183267506235 66-67 0.02787751108084291 68-69 0.04230271983475559 70-71 0.053696950873838356 72-73 0.08438092280174599 74-75 0.08458672993053074 76-77 0.09057384640426881 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2672405.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.3402279078163 #Duplication Level Percentage of deduplicated Percentage of total 1 76.9855294298463 44.143678031112856 2 12.70528921810925 14.570483588022112 3 4.444449976815366 7.645373237864457 4 2.0079856711160726 4.605534240697002 5 1.0734113679850985 3.0774826239553206 6 0.6237479910459429 2.1459511178170154 7 0.4392086425382819 1.7629026563559396 8 0.28217785375919074 1.2944113956072365 9 0.2091679946905126 1.079436643791741 >10 1.1024006781415268 11.569283609783884 >50 0.08607459723064376 3.3846605008318034 >100 0.03898440215000296 3.8662653096099406 >500 0.0013101471432551697 0.5129100370785722 >1k 1.9652207147712885E-4 0.13311996860705938 >5k 6.550735715904295E-5 0.20850703886506394 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5551 0.20771552216075034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.741947796086297E-5 0.0 0.0 0.0 0.0 2 3.741947796086297E-5 0.0 0.0 4.116142575694926E-4 0.0 3 3.741947796086297E-5 0.0 0.0 8.232285151389852E-4 0.0 4 3.741947796086297E-5 0.0 0.0 0.0018335544200822854 0.0 5 3.741947796086297E-5 0.0 0.0 0.0030683971927907634 0.0 6 3.741947796086297E-5 0.0 0.0 0.0055006632602468564 0.0 7 3.741947796086297E-5 0.0 0.0 0.01122584338825889 0.0 8 3.741947796086297E-5 0.0 0.0 0.01814844681101854 0.0 9 3.741947796086297E-5 0.0 0.0 0.02522072814562164 0.0 10 3.741947796086297E-5 0.0 0.0 0.034014305466424435 0.0 11 3.741947796086297E-5 0.0 0.0 0.043294336000718456 0.0 12 3.741947796086297E-5 0.0 0.0 0.05717696232419862 0.0 13 3.741947796086297E-5 0.0 0.0 0.07783251415859498 0.0 14 3.741947796086297E-5 0.0 0.0 0.10148162422986037 0.0 15 3.741947796086297E-5 0.0 0.0 0.12673977185344287 0.0 16 3.741947796086297E-5 0.0 0.0 0.15233469477867315 0.0 17 3.741947796086297E-5 0.0 0.0 0.1789773630868076 0.0 18 3.741947796086297E-5 0.0 0.0 0.20606906513047238 0.0 19 7.483895592172594E-5 0.0 0.0 0.23570529167547583 0.0 20 7.483895592172594E-5 0.0 0.0 0.26653894151522695 0.0 21 7.483895592172594E-5 0.0 0.0 0.3007777638494165 0.0 22 7.483895592172594E-5 3.741947796086297E-5 0.0 0.33976885988463573 0.0 23 7.483895592172594E-5 3.741947796086297E-5 0.0 0.3787225364418941 0.0 24 7.483895592172594E-5 3.741947796086297E-5 0.0 0.4179007298669176 0.0 25 7.483895592172594E-5 3.741947796086297E-5 0.0 0.46123248534559697 0.0 26 7.483895592172594E-5 3.741947796086297E-5 0.0 0.5052377914275717 0.0 27 7.483895592172594E-5 3.741947796086297E-5 0.0 0.5488314832519772 0.0 28 7.483895592172594E-5 3.741947796086297E-5 0.0 0.5950819580116038 0.0 29 7.483895592172594E-5 3.741947796086297E-5 0.0 0.6412201743373478 0.0 30 7.483895592172594E-5 3.741947796086297E-5 0.0 0.6939067993062429 0.0 31 7.483895592172594E-5 3.741947796086297E-5 0.0 0.7496618214679287 0.0 32 7.483895592172594E-5 3.741947796086297E-5 0.0 0.8083355629105619 3.741947796086297E-5 33 7.483895592172594E-5 3.741947796086297E-5 0.0 0.8705641547594769 3.741947796086297E-5 34 7.483895592172594E-5 3.741947796086297E-5 0.0 0.936908889184087 3.741947796086297E-5 35 7.483895592172594E-5 3.741947796086297E-5 0.0 1.0059852454998401 3.741947796086297E-5 36 7.483895592172594E-5 3.741947796086297E-5 0.0 1.0779429016185795 3.741947796086297E-5 37 7.483895592172594E-5 3.741947796086297E-5 0.0 1.1534179886656402 3.741947796086297E-5 38 7.483895592172594E-5 3.741947796086297E-5 0.0 1.2256001616521448 3.741947796086297E-5 39 7.483895592172594E-5 3.741947796086297E-5 0.0 1.3001023422722229 3.741947796086297E-5 40 7.483895592172594E-5 3.741947796086297E-5 0.0 1.3797309913729394 3.741947796086297E-5 41 7.483895592172594E-5 3.741947796086297E-5 0.0 1.470735161773758 3.741947796086297E-5 42 7.483895592172594E-5 3.741947796086297E-5 0.0 1.5655935384045456 3.741947796086297E-5 43 7.483895592172594E-5 3.741947796086297E-5 0.0 1.6631461174485154 3.741947796086297E-5 44 7.483895592172594E-5 3.741947796086297E-5 0.0 1.7711761503215269 3.741947796086297E-5 45 7.483895592172594E-5 3.741947796086297E-5 0.0 1.8796177974521078 3.741947796086297E-5 46 7.483895592172594E-5 3.741947796086297E-5 0.0 1.9982001231100825 3.741947796086297E-5 47 7.483895592172594E-5 3.741947796086297E-5 0.0 2.1137888905311883 3.741947796086297E-5 48 7.483895592172594E-5 3.741947796086297E-5 0.0 2.23398025374148 3.741947796086297E-5 49 7.483895592172594E-5 3.741947796086297E-5 0.0 2.3567909804090323 3.741947796086297E-5 50 7.483895592172594E-5 3.741947796086297E-5 0.0 2.4826701042693755 3.741947796086297E-5 51 7.483895592172594E-5 7.483895592172594E-5 0.0 2.6140124719120044 3.741947796086297E-5 52 7.483895592172594E-5 7.483895592172594E-5 0.0 2.75777810623764 3.741947796086297E-5 53 7.483895592172594E-5 7.483895592172594E-5 0.0 2.9108986100534913 3.741947796086297E-5 54 7.483895592172594E-5 7.483895592172594E-5 0.0 3.076442380552349 3.741947796086297E-5 55 7.483895592172594E-5 7.483895592172594E-5 0.0 3.24733713639961 3.741947796086297E-5 56 7.483895592172594E-5 7.483895592172594E-5 0.0 3.4265764358321436 3.741947796086297E-5 57 7.483895592172594E-5 7.483895592172594E-5 0.0 3.6032337912853776 3.741947796086297E-5 58 7.483895592172594E-5 7.483895592172594E-5 0.0 3.784830517829446 3.741947796086297E-5 59 7.483895592172594E-5 7.483895592172594E-5 0.0 3.96713821445477 3.741947796086297E-5 60 7.483895592172594E-5 7.483895592172594E-5 0.0 4.155058832774224 3.741947796086297E-5 61 7.483895592172594E-5 7.483895592172594E-5 0.0 4.353606582834563 3.741947796086297E-5 62 7.483895592172594E-5 7.483895592172594E-5 0.0 4.562369850378217 3.741947796086297E-5 63 7.483895592172594E-5 7.483895592172594E-5 0.0 4.781386054883148 3.741947796086297E-5 64 7.483895592172594E-5 7.483895592172594E-5 0.0 5.007960993936174 3.741947796086297E-5 65 7.483895592172594E-5 7.483895592172594E-5 0.0 5.244414675170867 3.741947796086297E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTTAC 265 0.0 26.785809 4 ATTCGCG 190 4.7293724E-10 22.420527 56 CGATCGT 230 1.0913936E-11 21.60455 1 CTCGCGC 205 1.2223609E-9 20.778055 36 TTAGTCG 120 5.1325966E-5 20.706686 15 CGCTCCG 230 2.3646862E-10 20.063995 25 CGGCGTA 90 0.0048589143 19.725082 46 CCTCGCG 225 3.8944563E-9 18.931118 35 ACGATCG 190 2.5944792E-7 18.683771 59 CAATTCG 250 7.185008E-10 18.460949 54 TTGCGTA 250 7.185008E-10 18.45784 36 CGCGCTT 235 6.681148E-9 18.124521 38 AATTCGC 255 9.349606E-10 18.097614 55 TCGCGCT 240 8.6602086E-9 17.748919 37 TCGAACT 650 0.0 17.477167 24 CGAACTC 650 0.0 17.475859 25 CGTAAGT 285 2.2009772E-10 17.435904 39 CGCGGAA 225 8.008283E-8 17.355148 59 CTCGAAC 635 0.0 17.3316 23 TGCGTAA 275 2.557499E-9 16.780796 37 >>END_MODULE