FastQCFastQC Report
Thu 26 May 2016
SRR936013_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936013_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2672405
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68280.25550019551677233No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50660.1895670753497318No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.13164172346631592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA9800.046.76706100-101
GGTATCA69750.038.479241
CGGTATC8800.037.20107100-101
CGTACAT14950.033.841778100-101
CCTGTCG7050.031.23220896-97
GTATCAA134950.030.4277861
TCGTATC4450.030.09525100-101
CGAGTAC10100.029.760857100-101
AGTATCA30300.028.189325100-101
TCGTACA9100.026.16339100-101
CGTAATA2050.026.131485100-101
GCGTACA6050.026.071493100-101
ACGTATC4050.025.719261100-101
GCGTATC3250.025.640123100-101
TACGCAG9900.025.552254100-101
TCGAGTA5250.024.942432100-101
ACCGTAT3700.024.93430598-99
GGGTATC14150.024.818243100-101
CGGGTAG2550.024.50894100-101
CCGTACA8000.024.180696100-101
Produced by FastQC (version 0.11.5)