FastQCFastQC Report
Thu 26 May 2016
SRR936012_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936012_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685854
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55390.20622863342534625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCGC2250.028.39341536
ATTCGCG2300.027.78185856
CGCGGAA2650.025.45783459
TCGCGGA2650.024.1161558
CGATCGT2800.024.0814591
CGCGCTT2850.023.66338238
TCGCGCT2700.023.66250237
CCTCGCG2900.022.02691335
CGCTCCG3000.021.29625125
GCGTTCG1003.5807476E-421.29585510
CCGGGTA3500.019.26480933
AATTCGC3350.019.07624455
CAATTCG3550.019.00267254
CGGTTAC2253.9035513E-918.9271814
TCGAACT6600.018.82594924
GCGTAAG2851.0913936E-1118.68161838
TTGCGTA2851.0913936E-1118.67987836
AACTCGA3450.018.52123813
CGTAAGT3101.8189894E-1218.32276739
CGTTCGG1200.0012214417.74687611
Produced by FastQC (version 0.11.5)