##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936012_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2685854 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.93031117849295 40.0 36.0 41.0 31.0 41.0 2 36.80871521683606 40.0 36.0 41.0 30.0 41.0 3 36.67379686312063 39.0 36.0 41.0 30.0 41.0 4 36.574963866241426 39.0 36.0 41.0 30.0 41.0 5 36.51096969529989 39.0 36.0 41.0 30.0 41.0 6 36.417011870339934 39.0 36.0 41.0 30.0 41.0 7 36.27786990655486 39.0 36.0 40.0 30.0 41.0 8 36.157607226602785 39.0 35.0 40.0 30.0 41.0 9 36.319799587021485 39.0 36.0 41.0 30.0 41.0 10-11 36.5373438020086 39.5 36.0 41.0 30.0 41.0 12-13 36.467039347633936 40.0 36.0 41.0 30.0 41.0 14-15 36.32355128015149 40.0 35.5 41.0 30.0 41.0 16-17 36.18206816156054 40.0 35.0 41.0 29.0 41.0 18-19 36.03534481025402 39.0 35.0 41.0 29.0 41.0 20-21 35.8390642231484 39.0 35.0 41.0 28.0 41.0 22-23 35.577967194047034 39.0 35.0 41.0 27.0 41.0 24-25 35.35978817165788 39.0 35.0 41.0 26.0 41.0 26-27 34.411972690995114 38.0 34.0 40.0 25.0 40.5 28-29 34.43141808899516 38.0 33.5 40.0 24.0 40.5 30-31 34.856072965991444 39.0 34.0 40.0 25.0 41.0 32-33 34.74918256911954 38.5 34.0 40.0 24.5 41.0 34-35 34.51016734342224 38.0 34.0 40.0 24.0 41.0 36-37 34.219765110091615 38.0 34.0 40.0 23.0 41.0 38-39 33.927582623627345 37.5 33.5 40.0 22.5 41.0 40-41 33.5803802068169 37.0 33.0 40.0 22.0 41.0 42-43 33.22118514260269 36.5 33.0 39.5 20.5 41.0 44-45 32.867691058411964 36.0 32.0 39.0 20.0 41.0 46-47 32.45496553423976 35.5 32.0 39.0 18.5 40.5 48-49 32.03530124869036 35.0 32.0 38.5 17.0 40.0 50-51 31.5765493954623 35.0 31.0 37.0 12.0 39.5 52-53 31.088837851945787 35.0 31.0 37.0 7.5 39.0 54-55 30.623069608400158 35.0 30.5 36.0 6.0 39.0 56-57 30.17981617764778 34.5 30.0 36.0 2.0 37.5 58-59 29.744734449452576 34.0 30.0 35.5 2.0 37.0 60-61 29.37561628442946 34.0 29.5 35.0 2.0 37.0 62-63 29.000134221741018 34.0 29.0 35.0 2.0 36.0 64-65 28.617894718030094 34.0 29.0 35.0 2.0 36.0 66-67 28.260221143814967 34.0 29.0 35.0 2.0 35.5 68-69 27.893474663924398 34.0 28.5 35.0 2.0 35.0 70-71 27.452154137938997 34.0 27.0 35.0 2.0 35.0 72-73 26.981452081907655 33.5 26.0 35.0 2.0 35.0 74-75 26.423922893798398 33.0 25.0 35.0 2.0 35.0 76-77 24.65899747342931 31.0 20.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.800374620574793 1101 2 0.6424676860183922 1101 3 0.7570429103424985 1101 4 0.7446479710127036 1101 5 0.7467569929946336 1101 6 0.7365905544547289 1101 7 0.8726440801886071 1101 8 0.7673684486106822 1101 9 0.7528375567087195 1101 10-11 0.7874553358667313 1101 12-13 0.8917447592012309 1101 14-15 0.9247110673787944 1101 16-17 0.9382158293574747 1101 18-19 0.9094529170054599 1101 20-21 0.8866844874732038 1101 22-23 0.8861859557571918 1101 24-25 0.9260920985477767 1101 26-27 0.8915426724494466 1101 28-29 0.9614361554427475 1101 30-31 0.9793464864323909 1101 32-33 1.0927612186918338 1101 34-35 1.1431335299850502 1101 36-37 1.1946834565599573 1101 38-39 1.117505357285033 1101 40-41 1.122187943033225 1101 42-43 1.102464300561941 1101 44-45 1.0771741008093088 1101 46-47 1.1007865100469658 1101 48-49 1.1218005701731784 1101 50-51 1.0019055566149326 1101 52-53 1.1727411195936845 1101 54-55 1.174159374617851 1101 56-57 1.1182161968192652 1101 58-59 1.0361321581859606 1101 60-61 0.9641097758302308 1101 62-63 1.0322580186264112 1101 64-65 1.0069936730220768 1101 66-67 0.9618206316501734 1101 68-69 1.0632710295774963 1101 70-71 1.0358092806563661 1101 72-73 0.973934255520085 1101 74-75 1.0928344895977666 1101 76-77 1.3260598924756586 1102 1 0.8522014863611815 1102 2 0.8528036799558407 1102 3 0.8264959351665695 1102 4 0.7648303967478043 1102 5 0.8426197398693205 1102 6 0.861828402577892 1102 7 0.8045927461798286 1102 8 0.8636732566435654 1102 9 0.7913874862809678 1102 10-11 0.8351265521938913 1102 12-13 0.9579262568824731 1102 14-15 0.9922269856990624 1102 16-17 0.9386784834267701 1102 18-19 0.9334100233445 1102 20-21 0.8765266908597837 1102 22-23 0.8241738361425845 1102 24-25 0.8532865477972393 1102 26-27 0.6124593041081638 1102 28-29 0.7063050884279178 1102 30-31 0.8835825855309665 1102 32-33 0.857787179488426 1102 34-35 0.8365908875576125 1102 36-37 0.872943245743464 1102 38-39 0.9160352980049069 1102 40-41 0.895048133641211 1102 42-43 0.9765997415110874 1102 44-45 0.8742927406887162 1102 46-47 0.8675155045912177 1102 48-49 0.7226241493309544 1102 50-51 0.6662939608839729 1102 52-53 0.756150646698071 1102 54-55 0.874763158771195 1102 56-57 0.8630816976525928 1102 58-59 0.8652487497571464 1102 60-61 0.7126058068898331 1102 62-63 0.7978749959714619 1102 64-65 0.7198323920880938 1102 66-67 0.7245724486313598 1102 68-69 0.6720839718102631 1102 70-71 0.7002951580379317 1102 72-73 0.8107471043983239 1102 74-75 0.7548553955211084 1102 76-77 0.9461721650269546 1103 1 0.4399056884128072 1103 2 0.5474436684500077 1103 3 0.5884836889555274 1103 4 0.5168640325553255 1103 5 0.44505277040126145 1103 6 0.5297933419276291 1103 7 0.5669670517734957 1103 8 0.5232406963989646 1103 9 0.565040061728844 1103 10-11 0.698274125001042 1103 12-13 0.6985620431316732 1103 14-15 0.6674274638989033 1103 16-17 0.5774583896935326 1103 18-19 0.5817735587333317 1103 20-21 0.6398714660670421 1103 22-23 0.5564678969131691 1103 24-25 0.6081795366041902 1103 26-27 0.7097507048252538 1103 28-29 0.6780432285084075 1103 30-31 0.790113719609117 1103 32-33 0.743668579326517 1103 34-35 0.8131554965163659 1103 36-37 0.7173831390200647 1103 38-39 0.6972781356788786 1103 40-41 0.6640999394657854 1103 42-43 0.7126189926535957 1103 44-45 0.8049432051978656 1103 46-47 0.7406035121300931 1103 48-49 0.7641813685911956 1103 50-51 0.6628522517508948 1103 52-53 0.7533664028411415 1103 54-55 0.7765657633182883 1103 56-57 0.747504567181764 1103 58-59 0.8517041916673662 1103 60-61 0.7158171317422273 1103 62-63 0.6479003322930978 1103 64-65 0.5368699417512239 1103 66-67 0.6063309514696691 1103 68-69 0.5828778843704292 1103 70-71 0.4733075210027913 1103 72-73 0.5064752830730086 1103 74-75 0.4003215771463182 1103 76-77 0.5395421992157452 1104 1 0.5555500854271571 1104 2 0.43009962295750626 1104 3 0.54218920224762 1104 4 0.6103553298483249 1104 5 0.6505675187947375 1104 6 0.5364720822974789 1104 7 0.5839770801509729 1104 8 0.48681526787522245 1104 9 0.5420503172892168 1104 10-11 0.6394768603996681 1104 12-13 0.6531143224314917 1104 14-15 0.6976397313677296 1104 16-17 0.7203487882160289 1104 18-19 0.47857616329790176 1104 20-21 0.5227407686184478 1104 22-23 0.6022989159420646 1104 24-25 0.6426014595210319 1104 26-27 0.49421107407902554 1104 28-29 0.5240437318059108 1104 30-31 0.7334883817837792 1104 32-33 0.6188222554516614 1104 34-35 0.7592571544563924 1104 36-37 0.8027999045938188 1104 38-39 0.8351810685809937 1104 40-41 0.797697902042934 1104 42-43 0.8165295385129383 1104 44-45 0.6951148360536124 1104 46-47 0.626909829824406 1104 48-49 0.704040739463494 1104 50-51 0.5573054793063186 1104 52-53 0.5891945005309545 1104 54-55 0.5189362042599086 1104 56-57 0.6531859454945952 1104 58-59 0.5881144309754269 1104 60-61 0.5754232694086596 1104 62-63 0.4701433294530233 1104 64-65 0.474782748371684 1104 66-67 0.41931194936764626 1104 68-69 0.23228697582232627 1104 70-71 0.2288360023113185 1104 72-73 0.16312437316180706 1104 74-75 0.09129712964493919 1104 76-77 0.22777415412168622 1105 1 0.18849429247106286 1105 2 0.480652727926838 1105 3 0.3249596665571275 1105 4 0.27761415770229547 1105 5 0.11064229996505048 1105 6 0.2406470771282727 1105 7 0.23594195252903205 1105 8 0.23516341959714282 1105 9 0.36030048385249813 1105 10-11 0.5042755772880341 1105 12-13 0.5488329281245115 1105 14-15 0.45138740522534704 1105 16-17 0.5095954052583238 1105 18-19 0.3701968814728289 1105 20-21 0.24687191135744513 1105 22-23 0.25686705755531847 1105 24-25 0.3054499501130792 1105 26-27 0.15902601652853576 1105 28-29 0.3003725047514365 1105 30-31 0.5820566554989384 1105 32-33 0.5315771259917881 1105 34-35 0.4978383799026673 1105 36-37 0.39838626478404393 1105 38-39 0.4525557727875835 1105 40-41 0.4331633889820736 1105 42-43 0.42518709589112547 1105 44-45 0.2431476662509553 1105 46-47 0.11450301910921468 1105 48-49 -0.06586285485727217 1105 50-51 0.030097941456247668 1105 52-53 -0.04409747915981299 1105 54-55 -0.1698324048792479 1105 56-57 -0.07360001494375368 1105 58-59 0.08992451437193338 1105 60-61 0.13510519097458484 1105 62-63 -0.08704717873729706 1105 64-65 0.09211796559317875 1105 66-67 0.1627143203098207 1105 68-69 0.167972297576636 1105 70-71 -0.0406306505707974 1105 72-73 -0.44629104715202317 1105 74-75 -0.6161301568668804 1105 76-77 -0.5407330368172723 1106 1 0.2610087662459577 1106 2 0.2989396532835116 1106 3 0.14491854265702386 1106 4 0.16607936320692573 1106 5 0.16838404646721727 1106 6 0.031544106558278884 1106 7 -0.1294421768619145 1106 8 -0.05829466389126736 1106 9 0.2046735166826963 1106 10-11 -0.03963610396727546 1106 12-13 0.23063517155961932 1106 14-15 0.25355330225477246 1106 16-17 0.28062172225696713 1106 18-19 0.3350962542759177 1106 20-21 0.28051180728410685 1106 22-23 0.41655213808004987 1106 24-25 0.24400403723142006 1106 26-27 0.1568342734724979 1106 28-29 0.35459718138892526 1106 30-31 0.46150202848582467 1106 32-33 0.35460694081936595 1106 34-35 0.27214570638967217 1106 36-37 0.292754415586991 1106 38-39 0.42028341884634557 1106 40-41 0.33939615829767433 1106 42-43 0.5028449289602506 1106 44-45 0.40720733036748413 1106 46-47 0.48513575855829316 1106 48-49 0.42181935963029105 1106 50-51 0.23433353086052833 1106 52-53 0.24442573631563747 1106 54-55 0.2170029214699163 1106 56-57 0.39295091895933965 1106 58-59 0.24410381687383165 1106 60-61 -0.009868618385127093 1106 62-63 0.009377597838160057 1106 64-65 0.10726948074469433 1106 66-67 0.06563492131609294 1106 68-69 0.02000494398001962 1106 70-71 -0.2578963706985391 1106 72-73 -0.3473001909018798 1106 74-75 -0.1712123472314815 1106 76-77 -0.18826072078354628 1107 1 -0.09655815508138943 1107 2 -0.041633319359206666 1107 3 0.07832296992043553 1107 4 -0.06183805674991305 1107 5 0.013381227703973764 1107 6 -0.21297430149310514 1107 7 -0.150596509009425 1107 8 -0.19582226138854253 1107 9 -0.29630124774923416 1107 10-11 -0.15313992387746822 1107 12-13 -0.011879276337694478 1107 14-15 -0.31037583153789683 1107 16-17 -0.14957459141167107 1107 18-19 -0.13061064433470193 1107 20-21 -0.0924658342303104 1107 22-23 -0.16573950255124004 1107 24-25 -0.12321513480200252 1107 26-27 -0.35262899512647294 1107 28-29 -0.16263532075638665 1107 30-31 0.08756572975801902 1107 32-33 0.14902426218892373 1107 34-35 -0.11645565939137015 1107 36-37 -0.07760024870247406 1107 38-39 -0.09302989154808472 1107 40-41 -0.0334637338950472 1107 42-43 -0.049633916429890235 1107 44-45 0.010172308275606667 1107 46-47 -0.06198507612887738 1107 48-49 -0.004051749296166918 1107 50-51 -0.0654356915773846 1107 52-53 -0.14376447882681376 1107 54-55 -0.14408939788623698 1107 56-57 -0.15025252909627085 1107 58-59 -0.051146914199492244 1107 60-61 -0.027407296537905523 1107 62-63 -0.2167487271888433 1107 64-65 -0.1430968196216078 1107 66-67 -0.0838807512852533 1107 68-69 -0.10997475537041268 1107 70-71 -0.1416879266280766 1107 72-73 -0.07640260226357753 1107 74-75 -0.2528309560676796 1107 76-77 -0.27204755563179006 1108 1 0.28981626904258206 1108 2 0.3841546253035091 1108 3 0.5275142505091708 1108 4 0.5175979337568535 1108 5 0.47613891768489935 1108 6 0.4665215202894686 1108 7 0.4169915593045914 1108 8 0.20601256440272664 1108 9 0.2573155528730098 1108 10-11 0.3540916829911609 1108 12-13 0.40729997151669295 1108 14-15 0.3674367370187781 1108 16-17 0.5515638648084575 1108 18-19 0.44932161336524956 1108 20-21 0.33437912963825056 1108 22-23 0.4486705278297549 1108 24-25 0.48264533866282733 1108 26-27 0.4268861056227422 1108 28-29 0.5214904445853463 1108 30-31 0.5329926259108646 1108 32-33 0.5469936551673911 1108 34-35 0.5626090448094416 1108 36-37 0.6286102377258018 1108 38-39 0.7007343599294202 1108 40-41 0.6352804076258565 1108 42-43 0.6945960218109306 1108 44-45 0.6120623117990007 1108 46-47 0.4877599761570153 1108 48-49 0.552893332240572 1108 50-51 0.4732103266512091 1108 52-53 0.5496796753251445 1108 54-55 0.5267677848584214 1108 56-57 0.48609929210128655 1108 58-59 0.5394027357875437 1108 60-61 0.49612234985925596 1108 62-63 0.40426235489302087 1108 64-65 0.4256905333762724 1108 66-67 0.46633995964326047 1108 68-69 0.40977920512764854 1108 70-71 0.5749594610719413 1108 72-73 0.4344973266036831 1108 74-75 0.5746655039151669 1108 76-77 0.3784999005418008 1109 1 -0.5953298763696608 1109 2 -0.5182418469611108 1109 3 -0.6548082108928668 1109 4 -0.3747321530877201 1109 5 -0.37927174110793516 1109 6 -0.5448820552551652 1109 7 -0.5273214764628733 1109 8 -0.5173919352760947 1109 9 -0.5152647713485194 1109 10-11 -0.5848629102362182 1109 12-13 -0.533094374705108 1109 14-15 -0.37106727401742035 1109 16-17 -0.427207223680071 1109 18-19 -0.4522009377793026 1109 20-21 -0.42345177250101074 1109 22-23 -0.42257387494853305 1109 24-25 -0.5800620406555339 1109 26-27 -0.6400943259692937 1109 28-29 -0.3669355091625377 1109 30-31 -0.3708862671045736 1109 32-33 -0.4001570201049418 1109 34-35 -0.2254089945036455 1109 36-37 -0.24873812480723245 1109 38-39 -0.25688340374596663 1109 40-41 -0.19341489384621013 1109 42-43 -0.39935049859681016 1109 44-45 -0.23874577143718057 1109 46-47 -0.3586160022300007 1109 48-49 -0.31833584636171963 1109 50-51 -0.2828770067008186 1109 52-53 -0.16379779356211444 1109 54-55 -0.0842518476513412 1109 56-57 -0.22764459986582253 1109 58-59 -0.11786614052136812 1109 60-61 -0.11831811493216549 1109 62-63 -0.20473439861822484 1109 64-65 -0.18025811307429151 1109 66-67 -0.3305812178532683 1109 68-69 -0.49862914733606445 1109 70-71 -0.3919066556547506 1109 72-73 -0.5327065264483011 1109 74-75 -0.501168453742924 1109 76-77 -0.2506509243013859 1110 1 -0.49388683632219 1110 2 -0.5265488054795142 1110 3 -0.7178071225243272 1110 4 -0.6661067150936972 1110 5 -0.676164284662228 1110 6 -0.6382186531726148 1110 7 -0.5275650165236456 1110 8 -0.9423842040525372 1110 9 -0.5739665307298978 1110 10-11 -0.5728504401507024 1110 12-13 -0.5270292102686369 1110 14-15 -0.5907248942398979 1110 16-17 -0.5437481829984421 1110 18-19 -0.6143224793930955 1110 20-21 -0.8443846925984886 1110 22-23 -0.8346216244454112 1110 24-25 -0.804371823612847 1110 26-27 -0.9164008245255175 1110 28-29 -0.6422452926413413 1110 30-31 -0.6028821639499782 1110 32-33 -0.5082496946598027 1110 34-35 -0.5883303592249689 1110 36-37 -0.5577546088710008 1110 38-39 -0.5198975431499662 1110 40-41 -0.39282035884345134 1110 42-43 -0.4695273232829038 1110 44-45 -0.5292955826805965 1110 46-47 -0.6070966147202199 1110 48-49 -0.5968752735158631 1110 50-51 -0.6844261978818516 1110 52-53 -0.6739011859656472 1110 54-55 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-0.4434663094497324 2314 18-19 -0.45378316287307285 2314 20-21 -0.49886626306975757 2314 22-23 -0.3122256992813419 2314 24-25 -0.44438282889652925 2314 26-27 -0.44323562426968977 2314 28-29 -0.5461223215604676 2314 30-31 -0.5815122654175369 2314 32-33 -0.6505209893075516 2314 34-35 -0.6851512727211357 2314 36-37 -1.1766192784532095 2314 38-39 -0.7032988096218773 2314 40-41 -0.561111370810977 2314 42-43 -0.5037874052175297 2314 44-45 -0.5963460514134908 2314 46-47 -0.35325104593142953 2314 48-49 -0.3160208371189128 2314 50-51 -0.10485586489999932 2314 52-53 -0.04784470754118786 2314 54-55 -0.28944899763608234 2314 56-57 -0.3041192314995591 2314 58-59 -0.18477060757928498 2314 60-61 -0.09948091397737358 2314 62-63 -0.29092097770255876 2314 64-65 -0.17622645421465322 2314 66-67 -0.1692332037108777 2314 68-69 -0.05032408084169049 2314 70-71 -0.14779620859001597 2314 72-73 -0.14433613214832874 2314 74-75 -0.1030462692098233 2314 76-77 -0.19602682839154895 2315 1 -0.8475773090299654 2315 2 -0.7319456257833679 2315 3 -0.6908112247796723 2315 4 -0.6778986938491656 2315 5 -0.8826121486489242 2315 6 -0.9512031831307795 2315 7 -1.0537495698855253 2315 8 -0.9895632075262881 2315 9 -0.6836419363549027 2315 10-11 -0.7942594568079144 2315 12-13 -0.6850771614530231 2315 14-15 -0.7515692586168115 2315 16-17 -0.614596076805995 2315 18-19 -0.6997522772246683 2315 20-21 -0.8030789117874519 2315 22-23 -0.9957501598921255 2315 24-25 -1.0952937552494646 2315 26-27 -0.7819557048127095 2315 28-29 -0.7917163263021152 2315 30-31 -1.045038782906417 2315 32-33 -0.8336967008249871 2315 34-35 -0.9744773203757475 2315 36-37 -1.0668131662775693 2315 38-39 -0.994856515878034 2315 40-41 -1.1857954235103563 2315 42-43 -1.0587570392014882 2315 44-45 -0.9781138840052677 2315 46-47 -0.8286422316675228 2315 48-49 -0.8388435775300707 2315 50-51 -0.860306267073792 2315 52-53 -0.8340815408149815 2315 54-55 -0.9590566210758347 2315 56-57 -1.0766187172800343 2315 58-59 -0.96964605819797 2315 60-61 -0.9844868680981662 2315 62-63 -0.8477256175865051 2315 64-65 -0.7286160052739454 2315 66-67 -0.7276409635541192 2315 68-69 -0.7659511580179235 2315 70-71 -0.6350885450952717 2315 72-73 -0.7530799750461483 2315 74-75 -0.7025679367966724 2315 76-77 -0.721857698884012 2316 1 -1.6839811603814852 2316 2 -1.3176416685519143 2316 3 -1.5447457676734473 2316 4 -2.4727963721775055 2316 5 -1.7894550421713973 2316 6 -1.6763708676829694 2316 7 -1.4702498538024358 2316 8 -1.3165294871236952 2316 9 -1.482068739103319 2316 10-11 -1.434131185874314 2316 12-13 -2.1562405017045023 2316 14-15 -3.317441949777198 2316 16-17 -2.246463084668882 2316 18-19 -1.87984120892839 2316 20-21 -1.4786200896583068 2316 22-23 -1.9432662588489436 2316 24-25 -1.8342624673577177 2316 26-27 -1.6522995294338827 2316 28-29 -2.163037432611013 2316 30-31 -2.304900556283691 2316 32-33 -2.3791061734834074 2316 34-35 -2.2249777704274507 2316 36-37 -2.2599830428171046 2316 38-39 -2.432350905729436 2316 40-41 -2.1002429509535965 2316 42-43 -2.2169472362571803 2316 44-45 -2.1823686702988994 2316 46-47 -2.1291305935537252 2316 48-49 -1.8318322555682904 2316 50-51 -2.077966642935152 2316 52-53 -2.081451465955144 2316 54-55 -2.123598064266414 2316 56-57 -1.854933602213098 2316 58-59 -1.9494842379558115 2316 60-61 -1.8069169861034595 2316 62-63 -1.8997259920096852 2316 64-65 -1.9784847091994386 2316 66-67 -1.89831073470965 2316 68-69 -1.9387866325063143 2316 70-71 -1.882974479925803 2316 72-73 -1.8818971077580855 2316 74-75 -2.018214548266915 2316 76-77 -2.698179710255566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 57824.0 3 4934.0 4 6600.0 5 8250.0 6 9417.0 7 9826.0 8 11584.0 9 12230.0 10 15019.0 11 16760.0 12 13111.0 13 11117.0 14 10954.0 15 11165.0 16 11591.0 17 12133.0 18 12927.0 19 13588.0 20 14937.0 21 16185.0 22 17855.0 23 20615.0 24 23894.0 25 28034.0 26 33144.0 27 39552.0 28 46881.0 29 55892.0 30 66934.0 31 82007.0 32 101588.0 33 127943.0 34 171255.0 35 230494.0 36 330814.0 37 462386.0 38 441861.0 39 123438.0 40 1115.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.278295506206597 26.995265046796618 29.01892975983433 21.707509687162457 2 21.98067794742102 27.077012670369328 28.926183593011118 22.016125789198536 3 22.275536239050684 27.344021126351485 28.39177436627648 21.988668268321344 4 21.736198286048158 27.482588079942712 28.783859821335806 21.997353812673325 5 22.21968181527054 27.257283975625995 28.65088751226967 21.87214669683379 6 22.28006363051405 27.18081968971188 28.679756202187747 21.859360477586332 7 22.111574851940563 26.891034807373774 28.746060188991766 22.251330151693896 8 21.952749935576897 27.32612092477318 28.535971924932564 22.185157214717364 9 21.97773143665748 27.046473523497433 28.843300811797125 22.132494228047964 10-11 22.467898068512298 27.170105092012538 28.445822061371977 21.91617477810319 12-13 22.459954184410446 26.942959162754825 28.402853950379665 22.19423270245506 14-15 22.199022807124678 27.231376217022905 28.537800131960505 22.03180084389191 16-17 22.38668425368876 27.00541852799604 28.5807850303041 22.0271121880111 18-19 22.26460136311226 27.038547661918223 28.390954463441414 22.305896511528108 20-21 22.293881131770558 27.27505453234742 28.31751789755738 22.11354643832464 22-23 22.39391095818997 27.310853180809392 27.883153104845317 22.412082756155325 24-25 22.389094373124944 27.419173441936262 27.82473998754681 22.36699219739198 26-27 22.43144474507149 27.40201258760945 27.80700264904312 22.35954001827594 28-29 22.575045514992905 27.34323322051784 27.771937072299163 22.309784192190094 30-31 22.370514041624066 27.286218126060728 27.871456454991662 22.471811377323547 32-33 22.34592885787586 27.639474830671617 27.71590067061115 22.298695640841373 34-35 22.37135020035743 27.325856227091734 27.83317765596238 22.469615916588456 36-37 22.405783281113536 27.308517433790954 28.04859317468122 22.237106110414288 38-39 22.333642470838452 27.263190958165524 28.094883181585363 22.308283389410665 40-41 22.196632481089452 27.29665340799497 27.962609354252294 22.544104756663284 42-43 22.144579390007987 27.54364732846884 27.75117345542359 22.560599826099583 44-45 22.178868134329566 27.283023765161023 28.023179582930613 22.514928517578795 46-47 22.125788004347918 27.22828023741511 28.00644442234985 22.63948733588712 48-49 22.325151783089403 27.169419698837476 27.846297151715316 22.659131366357805 50-51 22.168552563441043 27.394311789972292 27.752839179777816 22.684296466808842 52-53 22.197376639134998 27.27504991566199 27.83787437677582 22.689699068427196 54-55 22.19985479690653 27.251741906435807 27.821437808554244 22.72696548810342 56-57 22.07862813998403 27.393874465245943 27.809445185160776 22.718052209609255 58-59 22.18654810048335 27.40500554848031 27.774984918560225 22.633461432476114 60-61 22.157506655115057 27.229228472143475 27.74482416661872 22.86844070612274 62-63 22.200775471952124 27.161516808688802 27.80513655668928 22.832571162669794 64-65 22.24791538151735 27.12302633183674 27.74550254373449 22.883555742911422 66-67 22.133104435759414 27.241666309790023 27.832840222914456 22.792389031536104 68-69 22.267809601629452 27.045254407185197 27.87724218555493 22.809693805630417 70-71 22.07756503054366 26.953076978669728 27.88797602700687 23.08138196377974 72-73 22.228822935161315 26.80430922856046 27.86352805560694 23.103339780671288 74-75 22.067801296243992 26.774212539599386 27.729218102702408 23.428768061454218 76-77 22.005197805371065 26.648347042001618 28.096559947090437 23.249895205536873 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5178.0 1 3219.5 2 1222.0 3 1231.0 4 1279.0 5 1331.0 6 1470.0 7 1635.0 8 1766.5 9 1820.0 10 1890.5 11 2040.0 12 2187.0 13 2255.0 14 2508.5 15 2885.5 16 3289.0 17 3569.0 18 3871.5 19 4705.0 20 5937.0 21 7755.5 22 8873.0 23 10479.5 24 14144.0 25 19387.5 26 22573.0 27 25908.5 28 33696.5 29 41424.0 30 44699.0 31 50607.0 32 61656.5 33 71559.5 34 80484.5 35 84648.0 36 89806.5 37 99786.0 38 106146.5 39 107686.0 40 111046.5 41 117916.5 42 126227.0 43 131028.0 44 130014.0 45 129210.0 46 130242.5 47 131629.5 48 132194.0 49 130714.0 50 126338.0 51 119164.0 52 114886.0 53 111736.0 54 103077.0 55 90341.0 56 77069.5 57 71025.0 58 66946.0 59 56088.5 60 45026.0 61 40742.0 62 35674.5 63 25789.0 64 18127.5 65 15284.0 66 13140.0 67 9715.0 68 7152.5 69 4923.0 70 3975.0 71 3287.5 72 2282.0 73 1771.0 74 1578.0 75 1395.0 76 997.0 77 632.5 78 483.0 79 397.0 80 266.0 81 182.5 82 121.5 83 99.0 84 82.0 85 67.5 86 55.5 87 41.0 88 46.0 89 45.5 90 34.5 91 29.0 92 30.0 93 28.0 94 22.0 95 28.0 96 37.0 97 30.5 98 26.0 99 56.5 100 85.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030902647723964147 2 0.008116599040752029 3 0.011095167496073876 4 0.0179086428376226 5 0.013775879105863535 6 0.013031236992033075 7 0.01660551913841929 8 0.01794587494331412 9 0.016903375983951474 10-11 0.020123953126268218 12-13 0.01949100732951233 14-15 0.00752088534968766 16-17 0.006012985069180975 18-19 0.011486104605834865 20-21 0.010871774861924735 22-23 0.013924807528629629 24-25 0.022264799203530795 26-27 0.013254629626182214 28-29 0.007465037191150375 30-31 0.007297492715538522 32-33 0.009084633788731629 34-35 0.010369141435089173 36-37 0.009326642475726529 38-39 0.015842260971743065 40-41 0.01176534539852129 42-43 0.015227931227832935 44-45 0.01762940204493617 46-47 0.016047037553046442 48-49 0.013422174101794065 50-51 0.026192786353986478 52-53 0.03071648719550653 54-55 0.024182252646644236 56-57 0.014539137272539759 58-59 0.01615873387012101 60-61 0.019118686272597097 62-63 0.024219484752335756 64-65 0.019751632069352987 66-67 0.01932346285390047 68-69 0.02898519428085071 70-71 0.0558481585372846 72-73 0.0670364062975873 74-75 0.07818742195219845 76-77 0.07554394244810031 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 2685854.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.212699731936134 #Duplication Level Percentage of deduplicated Percentage of total 1 76.95302323721408 44.026902119354325 2 12.730900247166616 14.567383463167502 3 4.4631038774377245 7.660386660368531 4 1.9667498529625829 4.5009227514151116 5 1.092758323648755 3.1259826925245053 6 0.6356399793008931 2.182000756401366 7 0.40901390659748077 1.6380552877043486 8 0.2976901978854861 1.362532792381037 9 0.22057741922119123 1.135784668819267 >10 1.1014740395801164 11.576235223695749 >50 0.08735300773034252 3.398547892297078 >100 0.04001641054275294 3.930759269081326 >500 0.0013073082399910239 0.4748337326189636 >1k 3.268270599710691E-4 0.21252895190173987 >5k 6.536541199421382E-5 0.20714373826917773 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5539 0.20622863342534625 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.723210569152307E-5 0.0 2 0.0 0.0 0.0 1.116963170745692E-4 0.0 3 0.0 0.0 0.0 2.9785684553218457E-4 0.0 4 0.0 0.0 0.0 0.0010052668536711228 0.0 5 0.0 0.0 0.0 0.002606247398406615 0.0 6 0.0 0.0 0.0 0.005324191113887799 0.0 7 0.0 0.0 0.0 0.011095167496073874 0.0 8 0.0 0.0 0.0 0.017573553886398887 0.0 9 0.0 0.0 0.0 0.024238100805181517 0.0 10 0.0 0.0 0.0 0.03242916405731659 0.0 11 0.0 0.0 0.0 0.04140210152897365 0.0 12 0.0 0.0 0.0 0.053204679033186465 0.0 13 0.0 0.0 0.0 0.07096439344804296 0.0 14 0.0 0.0 0.0 0.09274517527758397 0.0 15 0.0 0.0 0.0 0.11616416975755198 0.0 16 0.0 0.0 0.0 0.14196601900177747 0.0 17 0.0 0.0 0.0 0.16780510035169446 0.0 18 0.0 0.0 0.0 0.1915591837828862 0.0 19 0.0 0.0 0.0 0.21866415672631498 0.0 20 0.0 0.0 0.0 0.24949234023889608 0.0 21 0.0 0.0 0.0 0.28579364328813106 0.0 22 0.0 0.0 0.0 0.3236959268821016 0.0 23 0.0 0.0 0.0 0.3629757983866584 0.0 24 0.0 0.0 0.0 0.4030375441107372 0.0 25 0.0 0.0 0.0 0.44216848719252794 0.0 26 0.0 0.0 0.0 0.48811290561586745 0.0 27 0.0 0.0 0.0 0.5321957187546308 0.0 28 0.0 0.0 0.0 0.5749754081941908 0.0 29 0.0 0.0 0.0 0.6199517918695506 0.0 30 0.0 0.0 0.0 0.6682046008457645 0.0 31 0.0 0.0 0.0 0.7208135661878866 0.0 32 0.0 0.0 0.0 0.7787094905382049 0.0 33 0.0 0.0 0.0 0.84092433914874 0.0 34 0.0 0.0 0.0 0.9071602551739596 0.0 35 0.0 0.0 0.0 0.974810991215457 0.0 36 0.0 0.0 0.0 1.0471157404683948 0.0 37 0.0 0.0 0.0 1.1218778086969732 0.0 38 0.0 0.0 0.0 1.1942197900556024 0.0 39 0.0 0.0 0.0 1.271625337788279 0.0 40 0.0 0.0 0.0 1.350780794488457 0.0 41 0.0 0.0 0.0 1.4371592796927906 0.0 42 0.0 0.0 0.0 1.5278939212630323 0.0 43 0.0 0.0 0.0 1.623543200784555 0.0 44 0.0 0.0 0.0 1.7297663983224703 0.0 45 0.0 0.0 0.0 1.8364736132343753 0.0 46 0.0 0.0 0.0 1.952749479309002 0.0 47 0.0 0.0 0.0 2.0695838269690014 3.723210569152307E-5 48 0.0 0.0 0.0 2.191705133637197 3.723210569152307E-5 49 0.0 0.0 0.0 2.3086139455085792 3.723210569152307E-5 50 0.0 0.0 0.0 2.435240336965449 3.723210569152307E-5 51 0.0 0.0 0.0 2.570095023780146 3.723210569152307E-5 52 0.0 0.0 0.0 2.711614257513625 3.723210569152307E-5 53 0.0 0.0 0.0 2.865643478759456 3.723210569152307E-5 54 0.0 0.0 0.0 3.0304700106558284 3.723210569152307E-5 55 0.0 0.0 0.0 3.196562434145713 3.723210569152307E-5 56 0.0 0.0 0.0 3.3721490445869358 3.723210569152307E-5 57 0.0 0.0 0.0 3.5514960977030023 3.723210569152307E-5 58 0.0 0.0 0.0 3.730917615030452 3.723210569152307E-5 59 0.0 0.0 0.0 3.9137272539758303 3.723210569152307E-5 60 0.0 0.0 0.0 4.102047244563554 3.723210569152307E-5 61 0.0 0.0 0.0 4.298818923143253 3.723210569152307E-5 62 0.0 0.0 0.0 4.497973456487211 3.723210569152307E-5 63 0.0 0.0 0.0 4.714589847400491 3.723210569152307E-5 64 0.0 0.0 0.0 4.940104711574047 3.723210569152307E-5 65 0.0 0.0 0.0 5.178204027471337 3.723210569152307E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGCGC 225 0.0 28.393415 36 ATTCGCG 230 0.0 27.781858 56 CGCGGAA 265 0.0 25.457834 59 TCGCGGA 265 0.0 24.11615 58 CGATCGT 280 0.0 24.081459 1 CGCGCTT 285 0.0 23.663382 38 TCGCGCT 270 0.0 23.662502 37 CCTCGCG 290 0.0 22.026913 35 CGCTCCG 300 0.0 21.296251 25 GCGTTCG 100 3.5807476E-4 21.295855 10 CCGGGTA 350 0.0 19.264809 33 AATTCGC 335 0.0 19.076244 55 CAATTCG 355 0.0 19.002672 54 CGGTTAC 225 3.9035513E-9 18.927181 4 TCGAACT 660 0.0 18.825949 24 GCGTAAG 285 1.0913936E-11 18.681618 38 TTGCGTA 285 1.0913936E-11 18.679878 36 AACTCGA 345 0.0 18.521238 13 CGTAAGT 310 1.8189894E-12 18.322767 39 CGTTCGG 120 0.00122144 17.746876 11 >>END_MODULE