FastQCFastQC Report
Thu 26 May 2016
SRR936012_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936012_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2685854
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69130.25738554664549895No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49430.18403829843319852No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35880.13358879522118478No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA12350.044.585148100-101
GGTATCA69850.037.1479151
CGTACAT15650.036.70527100-101
CGGTATC8900.036.117626100-101
GTATCAA137000.031.0598831
ACGTATC5350.030.041763100-101
CGAGTAC10050.029.615522100-101
TCGTATC5100.029.18100-101
CCTGTCG7700.028.21165796-97
ACCGTAC3300.027.95662198-99
GCGTATC4600.027.822496100-101
CCGTACA7350.027.536392100-101
TCGTACA9150.027.32396100-101
TACGCAG8900.027.08822100-101
GCGTACA4850.027.00203100-101
ACCGTAT3100.026.88024798-99
CGAGCCG4750.026.31189796-97
AGCGTAC3000.025.79223898-99
TCGGTAT3950.025.6193100-101
TCGAGTA5150.025.429094100-101
Produced by FastQC (version 0.11.5)