FastQCFastQC Report
Thu 26 May 2016
SRR936011_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936011_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752377
Sequences flagged as poor quality0
Sequence length77
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133970.7645044416812136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA56340.3215061599187846No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGAA3100.027.48272159
TCGCGGA3250.027.3057758
CGGTTAC3650.027.2255214
CGATAAT700.001130837625.3517422
ATTCGCG3550.024.99895556
AATTCGC3650.024.3140555
CTCGCGC3800.024.28707136
TCGCGCT3950.024.26411637
CGATCGT3800.023.351621
CAATTCG3750.022.72099754
CCTCGCG4550.022.62349135
CGCGCTT4350.022.0335638
GCCTAGT4550.021.84648717
ATCGTTC4200.021.1258493
AACTCGA4700.020.39160213
ACTCGAG5100.019.48882514
ATAACGC950.006640331418.68662851
TAACGCG950.0066425218.68556452
CGCTCCG4750.018.68236425
GATCGTT4750.018.6802312
Produced by FastQC (version 0.11.5)