##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936011_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1752377 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.91550733660622 40.0 36.0 41.0 31.0 41.0 2 36.7718002461799 40.0 36.0 41.0 30.0 41.0 3 36.68074620929172 40.0 36.0 41.0 30.0 41.0 4 36.56093123796991 39.0 36.0 41.0 30.0 41.0 5 36.468006028383165 39.0 36.0 41.0 30.0 41.0 6 36.32688856336279 39.0 36.0 41.0 30.0 41.0 7 36.12264883640906 39.0 35.0 40.0 30.0 41.0 8 35.912633525776705 39.0 35.0 40.0 29.0 41.0 9 35.78588796817123 38.0 35.0 40.0 29.0 41.0 10-11 35.95005869170846 39.0 35.0 40.5 29.5 41.0 12-13 36.06935436838078 39.0 35.0 41.0 30.0 41.0 14-15 35.977871200089936 39.0 35.0 41.0 29.0 41.0 16-17 35.76222810502534 39.0 35.0 41.0 28.0 41.0 18-19 35.525941050356174 39.0 35.0 41.0 27.0 41.0 20-21 35.20049452828928 39.0 35.0 41.0 25.5 41.0 22-23 34.770282593300415 39.0 34.5 41.0 23.5 41.0 24-25 34.41822992426858 39.0 34.0 41.0 21.5 41.0 26-27 33.440248873387404 38.0 33.0 40.0 19.0 40.5 28-29 33.4325795761985 38.0 33.0 39.5 18.0 40.5 30-31 33.75418474449276 38.0 33.0 40.0 18.0 41.0 32-33 33.57786109952367 38.0 33.0 40.0 16.0 41.0 34-35 33.29969121941226 37.5 33.0 40.0 12.5 41.0 36-37 32.99696811816179 37.0 33.0 40.0 9.5 41.0 38-39 32.70671664830114 36.5 32.5 40.0 7.5 41.0 40-41 32.31498159357261 36.0 32.0 39.5 6.0 41.0 42-43 31.94870310441189 35.5 32.0 39.0 2.0 41.0 44-45 31.582392373330624 35.0 31.0 39.0 2.0 40.5 46-47 31.193188166701574 35.0 31.0 38.0 2.0 40.0 48-49 30.751088378813463 35.0 31.0 37.0 2.0 39.5 50-51 30.319259211916158 35.0 30.0 37.0 2.0 39.0 52-53 29.882042505693697 35.0 30.0 36.0 2.0 39.0 54-55 29.439123544762342 34.0 29.5 36.0 2.0 37.5 56-57 29.01692215773204 34.0 29.0 35.0 2.0 37.0 58-59 28.607050024053045 34.0 29.0 35.0 2.0 37.0 60-61 28.2670700996418 34.0 29.0 35.0 2.0 36.0 62-63 27.934229050027476 34.0 27.5 35.0 2.0 36.0 64-65 27.567853264451657 34.0 27.0 35.0 2.0 35.5 66-67 27.201230100600498 34.0 26.0 35.0 2.0 35.0 68-69 26.852662412254897 33.5 25.5 35.0 2.0 35.0 70-71 26.430978037260246 33.0 25.0 35.0 2.0 35.0 72-73 25.957554224918496 33.0 23.5 35.0 2.0 35.0 74-75 25.40063724871988 33.0 19.5 35.0 2.0 35.0 76-77 23.774779057246242 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7694336292824318 1101 2 0.6745442244231938 1101 3 0.75372775661954 1101 4 0.8162457033944079 1101 5 0.9781617720379217 1101 6 0.9585421469028788 1101 7 0.8529253080023267 1101 8 1.1013559425923702 1101 9 1.04499658031569 1101 10-11 0.9971881451427933 1101 12-13 1.025231099486021 1101 14-15 1.1343774009130456 1101 16-17 1.0171642545445039 1101 18-19 1.0708606788118118 1101 20-21 1.11322205600694 1101 22-23 1.1521133097994607 1101 24-25 1.156154029041872 1101 26-27 0.8999123581723651 1101 28-29 1.0256154722033841 1101 30-31 1.130333684280501 1101 32-33 1.2116781807086667 1101 34-35 1.1717355333969053 1101 36-37 1.1657005332580326 1101 38-39 1.2048581270188023 1101 40-41 1.1915983874484155 1101 42-43 0.9140076184997774 1101 44-45 0.7749740907938083 1101 46-47 0.8576528197063027 1101 48-49 0.9758248192596177 1101 50-51 0.8261421696473015 1101 52-53 0.976387910145764 1101 54-55 0.83372519631261 1101 56-57 0.8155409849520083 1101 58-59 0.7522155996884621 1101 60-61 0.5896872329113307 1101 62-63 0.4944361362714389 1101 64-65 0.39341757327137117 1101 66-67 0.454422559517063 1101 68-69 0.3028803908633826 1101 70-71 0.3591598380806289 1101 72-73 0.3386234818791145 1101 74-75 0.2940116865647049 1101 76-77 0.5152170602326933 1102 1 0.6819651185210702 1102 2 0.7673565840289385 1102 3 0.6719661417513407 1102 4 0.7966520759978195 1102 5 0.851820307736034 1102 6 0.8959700058529165 1102 7 0.7782780329468935 1102 8 0.8200515923938383 1102 9 0.7962598785508348 1102 10-11 0.7537365426247433 1102 12-13 0.9597636764107023 1102 14-15 0.8960559296630635 1102 16-17 0.9555453848285609 1102 18-19 0.9684186599355513 1102 20-21 0.8606680854981406 1102 22-23 0.8386661998976948 1102 24-25 0.9411848862221461 1102 26-27 0.8148844111888778 1102 28-29 0.8501395186137515 1102 30-31 1.0261600983178312 1102 32-33 0.8402275749330173 1102 34-35 1.0513141369026044 1102 36-37 0.8899004565015929 1102 38-39 0.8282026342952022 1102 40-41 0.8034699895663096 1102 42-43 0.865961906583788 1102 44-45 0.7880306886646373 1102 46-47 0.7116475195104108 1102 48-49 0.7012733988107556 1102 50-51 0.866141915005084 1102 52-53 0.9680663843295854 1102 54-55 0.8525505317582578 1102 56-57 0.8253867263337327 1102 58-59 0.9176250007153968 1102 60-61 0.9146652700199773 1102 62-63 0.7248675183784563 1102 64-65 0.6078106790151949 1102 66-67 0.5757641238569668 1102 68-69 0.6364391431892678 1102 70-71 0.6621360599540296 1102 72-73 0.613518305548471 1102 74-75 0.6162186706732093 1102 76-77 0.7798705376450208 1103 1 0.17554394167165555 1103 2 0.07814477071298143 1103 3 0.24257577973789068 1103 4 0.3009508490626871 1103 5 0.3930440300356892 1103 6 0.18324178648837375 1103 7 0.35265342267279465 1103 8 0.3604470070837138 1103 9 0.291806201205695 1103 10-11 0.27116524631616556 1103 12-13 0.3775446439098573 1103 14-15 0.33544478924842736 1103 16-17 0.3957254395398948 1103 18-19 0.3408876154324787 1103 20-21 0.4113348731240123 1103 22-23 0.5008074145122023 1103 24-25 0.43861085757417584 1103 26-27 0.40918830828653796 1103 28-29 0.46137352125071374 1103 30-31 0.7166176002209639 1103 32-33 0.6345978800693501 1103 34-35 0.7622181073646459 1103 36-37 0.5722426952772182 1103 38-39 0.6411302570631179 1103 40-41 0.6521552215587789 1103 42-43 0.5964282796255667 1103 44-45 0.4993624387710405 1103 46-47 0.44874322202822015 1103 48-49 0.5621144931951534 1103 50-51 0.48412805896064626 1103 52-53 0.4872347132889061 1103 54-55 0.38201318243584126 1103 56-57 0.5465803570426466 1103 58-59 0.49848126998206155 1103 60-61 0.5838230748111819 1103 62-63 0.5664945655263729 1103 64-65 0.4499558666376231 1103 66-67 0.4515597563451266 1103 68-69 0.37891633514758283 1103 70-71 0.3302581216582823 1103 72-73 0.49460990740574573 1103 74-75 0.36920120557418556 1103 76-77 0.3704033043824815 1104 1 0.5152942935720972 1104 2 0.49212313263654295 1104 3 0.3455135000669145 1104 4 0.4245089515368079 1104 5 0.5068372753357835 1104 6 0.17462590437636294 1104 7 0.5640473061333893 1104 8 0.5659740350050271 1104 9 0.48668660871266667 1104 10-11 0.478095941156333 1104 12-13 0.5986631418002446 1104 14-15 0.5842753351010117 1104 16-17 0.6126107584502236 1104 18-19 0.7398734982778024 1104 20-21 0.72714383276859 1104 22-23 0.7221476946374707 1104 24-25 0.8438780067960607 1104 26-27 0.5691501760506625 1104 28-29 0.6070313291479437 1104 30-31 0.7013723967089405 1104 32-33 0.7190060523869874 1104 34-35 0.7969407990855615 1104 36-37 0.6838147199522808 1104 38-39 0.6821106060839739 1104 40-41 0.8211466668456353 1104 42-43 0.7234130964853129 1104 44-45 0.5879740998663188 1104 46-47 0.49257293619741915 1104 48-49 0.6247438289037 1104 50-51 0.7367923969138452 1104 52-53 0.7082977656205713 1104 54-55 0.653914267559081 1104 56-57 0.5263055259280272 1104 58-59 0.5121205197142267 1104 60-61 0.45143750281870254 1104 62-63 0.6214096354503766 1104 64-65 0.49382492926785204 1104 66-67 0.4693858611873125 1104 68-69 0.3094280261119273 1104 70-71 0.23908326604030705 1104 72-73 0.3208059664785168 1104 74-75 0.20921268817054184 1104 76-77 0.2649562253797164 1105 1 0.1564702547009702 1105 2 0.3315089575432637 1105 3 0.2831111330147493 1105 4 0.29201766095252424 1105 5 0.15124990456300935 1105 6 -0.046266795268543603 1105 7 0.10547372372225539 1105 8 0.30217533341939173 1105 9 0.1587229770409735 1105 10-11 0.15606425673500013 1105 12-13 0.13445013538593997 1105 14-15 -0.09678665684973708 1105 16-17 0.06804188115621912 1105 18-19 0.35496186504362726 1105 20-21 0.2694262564518439 1105 22-23 0.33349759662892353 1105 24-25 0.40269073358994945 1105 26-27 0.21929802843512647 1105 28-29 0.38009020320740916 1105 30-31 0.3804393977888836 1105 32-33 0.5330342654146136 1105 34-35 0.5280355783851576 1105 36-37 0.5279162670555877 1105 38-39 0.5384376806570685 1105 40-41 0.5672138641194309 1105 42-43 0.3802257066234098 1105 44-45 0.3750553541635888 1105 46-47 0.2274143108269051 1105 48-49 0.30471279139178975 1105 50-51 0.3059676934581823 1105 52-53 0.48784853475344647 1105 54-55 0.2862296906926325 1105 56-57 0.25507072432241884 1105 58-59 0.3610816644964423 1105 60-61 0.23023018602574652 1105 62-63 0.14470562125372055 1105 64-65 0.1387291036011007 1105 66-67 0.1207092858479939 1105 68-69 0.012396562629607644 1105 70-71 -0.1713763401226558 1105 72-73 -0.10571502184352966 1105 74-75 -0.22196092216728402 1105 76-77 0.045816897705950765 1106 1 -0.05554420960173445 1106 2 0.06370789104764896 1106 3 -0.2307124264584175 1106 4 0.09746574066126357 1106 5 -0.0626363899462703 1106 6 0.09936201773523123 1106 7 0.08168410460850595 1106 8 0.132723419119678 1106 9 0.09596714673571682 1106 10-11 -0.19124465692677717 1106 12-13 -0.1752799629811861 1106 14-15 -0.13306565858728447 1106 16-17 0.08505505903823973 1106 18-19 0.036345419300623405 1106 20-21 -0.012514688027650323 1106 22-23 0.03469467275331084 1106 24-25 0.2142921334976151 1106 26-27 0.15824855586760833 1106 28-29 0.19572691095729766 1106 30-31 0.12534517187931726 1106 32-33 0.008116120538979033 1106 34-35 0.10733442624565015 1106 36-37 0.22961680946694685 1106 38-39 0.14314439436326865 1106 40-41 0.0784527753493478 1106 42-43 0.16025922405000514 1106 44-45 -0.015314817793854019 1106 46-47 0.09790011966831713 1106 48-49 0.21904351781416764 1106 50-51 0.00808764425699593 1106 52-53 -0.053136123358228815 1106 54-55 0.19550496004798035 1106 56-57 0.2769987341062681 1106 58-59 0.21051652050543623 1106 60-61 0.056436513805735444 1106 62-63 -0.11627265762113836 1106 64-65 -0.10402711439257217 1106 66-67 -0.025445197416200216 1106 68-69 0.09695387531442279 1106 70-71 -0.07700258028506113 1106 72-73 -0.006735347290341309 1106 74-75 -0.05196765384812352 1106 76-77 0.08241679972651639 1107 1 0.22142334525988616 1107 2 0.14527198349313153 1107 3 0.1651371351633273 1107 4 0.2516753729725707 1107 5 0.23386516282212 1107 6 -0.10219418218584053 1107 7 -0.002568593433800004 1107 8 0.05740299978642582 1107 9 -0.06232429330682976 1107 10-11 0.0676198490183495 1107 12-13 0.32172345139350256 1107 14-15 0.34138057787960463 1107 16-17 0.1820730574254199 1107 18-19 0.1715237731945507 1107 20-21 0.13076808739571533 1107 22-23 0.1383398179929216 1107 24-25 -0.13092688405468067 1107 26-27 -0.04878179603603883 1107 28-29 0.1448227021828714 1107 30-31 0.1922334583436438 1107 32-33 0.2786169526855673 1107 34-35 0.21225181210430577 1107 36-37 0.2318558843258245 1107 38-39 0.29662225240077333 1107 40-41 0.27886541711453106 1107 42-43 0.25953259707472753 1107 44-45 0.317842363545477 1107 46-47 0.22887227278328837 1107 48-49 0.24777402242100166 1107 50-51 -0.010193844341738867 1107 52-53 0.0764848638936293 1107 54-55 0.20451787976727687 1107 56-57 0.2019631319581272 1107 58-59 0.2305395117721858 1107 60-61 0.19703840458005928 1107 62-63 0.21088191575360327 1107 64-65 0.2986348968285384 1107 66-67 0.28750283202634463 1107 68-69 -0.05865753796217987 1107 70-71 -0.13645473614525727 1107 72-73 -0.032720626016558896 1107 74-75 0.1271274510709759 1107 76-77 0.20022645262701744 1108 1 0.007041462427700651 1108 2 0.1641404167020255 1108 3 0.09098806240894675 1108 4 -0.11165268342000445 1108 5 -0.047567732081276404 1108 6 -0.19483753496659517 1108 7 -0.3150841489016827 1108 8 0.11477205850184191 1108 9 -0.07351042136388486 1108 10-11 -0.378393594851282 1108 12-13 -0.21938950637289167 1108 14-15 -0.05810422173637164 1108 16-17 -0.030235530179190562 1108 18-19 -0.0720677028200214 1108 20-21 -0.0607412984900364 1108 22-23 -0.030707225687329753 1108 24-25 0.024330576405255044 1108 26-27 -0.26917972011055724 1108 28-29 -0.21677974526554067 1108 30-31 -0.11472700734227459 1108 32-33 -0.01352665243486939 1108 34-35 -0.04128365600637096 1108 36-37 0.010111929382020435 1108 38-39 -0.19014857219930548 1108 40-41 -0.057201733189508275 1108 42-43 0.001333529827430624 1108 44-45 -0.15843030798386692 1108 46-47 -0.13061479542404797 1108 48-49 -0.07491452135705856 1108 50-51 -0.024720062188976044 1108 52-53 -0.19260623648824904 1108 54-55 0.04032890939540934 1108 56-57 -0.09982215080205847 1108 58-59 -0.17324229576907157 1108 60-61 -0.20861170301363785 1108 62-63 -0.13974876908152112 1108 64-65 -0.19174101533423737 1108 66-67 -0.3556507072227504 1108 68-69 -0.3583726489176655 1108 70-71 -0.39457621099266404 1108 72-73 -0.20289243071385954 1108 74-75 -0.20040880940276296 1108 76-77 -0.051060479793616054 1109 1 -0.10368277649384794 1109 2 -0.20292306129134374 1109 3 -0.4355469011229758 1109 4 -0.3065697434029815 1109 5 -0.3160838211580028 1109 6 -0.2558018158688995 1109 7 -0.4401413439646191 1109 8 -0.04391059948231657 1109 9 0.06572709711160485 1109 10-11 -0.24299401283533228 1109 12-13 -0.08744509710376036 1109 14-15 0.020929646858327544 1109 16-17 -0.0084737162540236 1109 18-19 -0.1618127082347769 1109 20-21 -0.07783683948694886 1109 22-23 0.0324381498955546 1109 24-25 0.04100739044692858 1109 26-27 -0.02025464961078427 1109 28-29 -0.0386855472340315 1109 30-31 0.03965069855404835 1109 32-33 -0.06349310362599425 1109 34-35 0.1266229827406704 1109 36-37 0.12150072850350568 1109 38-39 -0.03160352004922373 1109 40-41 0.011004329098824428 1109 42-43 0.10977249885413798 1109 44-45 -0.06608649398238242 1109 46-47 -0.1869470782254794 1109 48-49 -0.0684478913627693 1109 50-51 -0.16754613820750208 1109 52-53 -0.1298677689546146 1109 54-55 -0.17374676604662298 1109 56-57 -0.03127418444744379 1109 58-59 -0.05121968924104792 1109 60-61 -0.1029069534680751 1109 62-63 -0.15105659181278952 1109 64-65 -0.06080003118406552 1109 66-67 0.02094471845540724 1109 68-69 -0.012323861149084792 1109 70-71 -0.12193549256526737 1109 72-73 -0.061865698712278316 1109 74-75 0.0767441984919266 1109 76-77 0.2671235486771124 1110 1 -0.2257896952581362 1110 2 -0.32444401882209206 1110 3 -0.32162542516883974 1110 4 -0.22673260721727218 1110 5 -0.16904194278050255 1110 6 -0.2127053333722344 1110 7 -0.4507711851458751 1110 8 -0.2552575471614489 1110 9 -0.3778874812370461 1110 10-11 -0.3547856883864 1110 12-13 -0.06104949236981838 1110 14-15 -0.23577459797062517 1110 16-17 -0.2709594561717452 1110 18-19 -0.17679091237658895 1110 20-21 -0.39047712708943294 1110 22-23 -0.4285083035978303 1110 24-25 -0.314542684345831 1110 26-27 -0.28501266792853386 1110 28-29 -0.1464406737397539 1110 30-31 -0.037686194436460596 1110 32-33 -0.05061849703722032 1110 34-35 0.07742729559876693 1110 36-37 0.06351562285852452 1110 38-39 0.057880031602977056 1110 40-41 0.24458111966239215 1110 42-43 0.08093790008691215 1110 44-45 0.018023180666656202 1110 46-47 -0.1669346515238601 1110 48-49 0.029966246646988992 1110 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2 -0.4706743829799649 2314 3 -0.36478671112350725 2314 4 -0.3423448458807883 2314 5 -0.3504826558713887 2314 6 -0.47083171941953594 2314 7 -0.588882181217464 2314 8 -0.4520956667441993 2314 9 -0.37988471038090665 2314 10-11 -0.49303869496346664 2314 12-13 -0.7269194140493767 2314 14-15 -0.9349162600549761 2314 16-17 -1.195542795697392 2314 18-19 -0.714815463681191 2314 20-21 -0.6775063663919951 2314 22-23 -0.552492969765801 2314 24-25 -0.7283575819083694 2314 26-27 -0.644592349858037 2314 28-29 -0.7747768885653201 2314 30-31 -0.8987929021210519 2314 32-33 -0.8927118970164187 2314 34-35 -0.9319833773163637 2314 36-37 -0.864479271368424 2314 38-39 -0.8147010423597294 2314 40-41 -0.6163084440516116 2314 42-43 -0.6465381604095661 2314 44-45 -0.6992978902246598 2314 46-47 -0.7189984274510977 2314 48-49 -0.6181808465135568 2314 50-51 -0.6673605691085562 2314 52-53 -0.6044886112607593 2314 54-55 -0.593023916916195 2314 56-57 -0.39626778922688644 2314 58-59 -0.5308852456950994 2314 60-61 -0.5827998502613845 2314 62-63 -0.46472353035638037 2314 64-65 -0.4762452893186868 2314 66-67 -0.4608642702497612 2314 68-69 -0.41875832047204753 2314 70-71 -0.399712057473117 2314 72-73 -0.22453091342809728 2314 74-75 -0.15425003271057847 2314 76-77 -0.018840033787466126 2315 1 -1.036322226304847 2315 2 -1.0264414135983984 2315 3 -0.8934983500334397 2315 4 -0.8281676314551305 2315 5 -1.0833282716965016 2315 6 -0.9403179306081597 2315 7 -0.9072152076520155 2315 8 -0.6024987103751442 2315 9 -0.616767302981323 2315 10-11 -0.803383045459988 2315 12-13 -0.99177230890988 2315 14-15 -1.2965280522130556 2315 16-17 -1.1063539845226487 2315 18-19 -1.0288405871199018 2315 20-21 -0.8691073013767436 2315 22-23 -0.8830415739800728 2315 24-25 -1.1789023524034263 2315 26-27 -1.2395392865553774 2315 28-29 -1.1883798500882605 2315 30-31 -1.252046230463499 2315 32-33 -1.235474318115763 2315 34-35 -1.0856569054348313 2315 36-37 -1.3382263445099447 2315 38-39 -1.2104779641749985 2315 40-41 -1.2404442221104617 2315 42-43 -1.4503973874472464 2315 44-45 -1.3488255176555732 2315 46-47 -1.3759097377277776 2315 48-49 -1.2771254870042732 2315 50-51 -1.2297913282151498 2315 52-53 -1.1321369213553147 2315 54-55 -1.3408575133775003 2315 56-57 -1.4846858316553941 2315 58-59 -1.2678166402331037 2315 60-61 -1.283216327002986 2315 62-63 -1.3566430866511752 2315 64-65 -1.2449122820024208 2315 66-67 -1.2868428497209514 2315 68-69 -1.3299800493083573 2315 70-71 -1.3822160597351107 2315 72-73 -2.4573926288614842 2315 74-75 -3.352030701709676 2315 76-77 -2.9797124514354536 2316 1 -2.6417885335632647 2316 2 -2.418532531784443 2316 3 -1.7729068467247089 2316 4 -1.924327450539522 2316 5 -2.6269641267668504 2316 6 -2.0225898753262825 2316 7 -2.524713258283917 2316 8 -2.2602220384765133 2316 9 -2.0617164353120927 2316 10-11 -2.1395739178445297 2316 12-13 -2.091912372418008 2316 14-15 -2.1809965918171486 2316 16-17 -3.4862642987435706 2316 18-19 -3.22356033836094 2316 20-21 -2.523530659587159 2316 22-23 -2.7331627822141584 2316 24-25 -2.8770914740683438 2316 26-27 -2.312329552364268 2316 28-29 -2.4417908730893174 2316 30-31 -2.750272943903127 2316 32-33 -2.838896683974589 2316 34-35 -2.820496077370585 2316 36-37 -2.771644066329621 2316 38-39 -2.638908837177155 2316 40-41 -2.557819164134205 2316 42-43 -2.697359164392026 2316 44-45 -2.387523814861016 2316 46-47 -2.236028695206315 2316 48-49 -2.4055465315092484 2316 50-51 -2.606286759300165 2316 52-53 -3.61727614454178 2316 54-55 -2.9253139974835456 2316 56-57 -2.7733363225754637 2316 58-59 -2.911122513161029 2316 60-61 -2.769260603343504 2316 62-63 -2.5572958651465427 2316 64-65 -2.5671581203316585 2316 66-67 -2.7336681560523424 2316 68-69 -2.7118712065708444 2316 70-71 -2.592083277035165 2316 72-73 -2.393929785034821 2316 74-75 -2.4997442547618824 2316 76-77 -2.60005957851525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 40206.0 3 4206.0 4 6202.0 5 7531.0 6 8414.0 7 8705.0 8 10805.0 9 12027.0 10 16955.0 11 19713.0 12 13126.0 13 9172.0 14 8649.0 15 8945.0 16 8474.0 17 9108.0 18 9215.0 19 9879.0 20 10637.0 21 11233.0 22 12798.0 23 14401.0 24 16479.0 25 19450.0 26 22637.0 27 26907.0 28 31596.0 29 38014.0 30 44806.0 31 54145.0 32 67103.0 33 85141.0 34 117996.0 35 157037.0 36 216336.0 37 282105.0 38 245225.0 39 66455.0 40 544.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.54569776951397 25.686401072012117 29.99396764902104 21.77393350945287 2 22.484403349277652 26.05717925347247 29.767558395766546 21.69085900148333 3 22.32724952443787 26.170213858847795 29.461623149331363 22.04091346738297 4 22.13678155212081 25.961464374322595 29.864624765922816 22.037129307633784 5 22.36447252924115 25.968037056103515 29.658523166342682 22.008967248312647 6 22.34188145403659 25.86996446748598 29.854616886904335 21.933537191573098 7 22.355409494739416 25.779784782253838 29.745514774959673 22.11929094804707 8 22.315668472364038 26.033890643344616 29.364223676647942 22.286217207643404 9 22.158627168735283 26.025222909287248 29.656609541464952 22.159540380512517 10-11 22.63340767662239 26.054112211277637 29.26143295619454 22.05104715590544 12-13 22.823617844370297 25.827272924440752 29.0153942566615 22.333714974527446 14-15 22.473510958011317 25.965824199279318 29.161259283862773 22.399405558846592 16-17 22.47041830407027 25.92077915111062 29.265505351356474 22.343297193462636 18-19 22.62231110761049 26.03507624414335 28.771098626823488 22.571514021422672 20-21 22.49638766575142 26.208894090612155 28.683318079194237 22.611400164442188 22-23 22.755848727173934 26.4605586730566 28.01410252641727 22.7694900733522 24-25 22.921059728518408 26.53168212710521 27.632020159213265 22.915237985163117 26-27 22.903666505610932 26.660070326085066 27.429884390902533 23.006378777401473 28-29 23.091238957598435 26.637933642007894 27.344179365297684 22.92664803509599 30-31 22.804070924438616 26.623943989120157 27.301679972560695 23.270305113880532 32-33 22.79328779964416 26.9529572404701 27.203844348405486 23.049910611480254 34-35 22.979136025897194 26.360364555956394 27.418259415954832 23.242240002191583 36-37 22.9767096245084 26.608864485045224 27.630932385487135 22.783493504959242 38-39 22.83743936269151 26.641748260748344 27.63286273160177 22.887949644958375 40-41 22.743509754772372 26.566716205295382 27.61961181804484 23.0701622218874 42-43 22.788165194164783 26.878267470264593 27.23506380841495 23.098503527155675 44-45 22.737081511751345 26.59833118358122 27.584211307315627 23.08037599735181 46-47 22.72091106478871 26.691693702867397 27.53665583696543 23.050739395378464 48-49 22.828050250243795 26.668222990807887 27.281011351938638 23.22271540700968 50-51 22.531296480186008 27.120837994136735 27.122978705063982 23.22488682061328 52-53 22.50805070014403 26.934208896330503 27.369012354206827 23.188728049318637 54-55 22.5358878030417 26.79390496000448 27.39799764082486 23.272209596128967 56-57 22.482361677365812 27.26898092418475 27.182423149652767 23.066234248796665 58-59 22.614234644504876 26.938455308519654 27.053775089043935 23.39353495793154 60-61 22.492025617338648 26.63151288814917 27.10194224769002 23.774519246822162 62-63 22.51247142752359 26.67616870668464 27.104616718548304 23.706743147243472 64-65 22.445081838549026 26.589722733564507 27.178874666438347 23.78632076144812 66-67 22.390328883490294 26.704680697552423 27.287791462625943 23.617198956331336 68-69 22.419147154875578 26.358223039988765 27.40549006667696 23.8171397384587 70-71 22.33277836395942 26.29244487989367 27.43155948677894 23.943217269367974 72-73 22.524560071871157 26.20978450646549 27.291992568440087 23.973662853223267 74-75 22.35461260250188 26.144901214102028 27.23480873216792 24.265677451228164 76-77 22.328302917039828 25.953184115292526 27.591962833330285 24.12655013433736 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14879.0 1 8690.5 2 2308.5 3 2153.0 4 2191.0 5 2230.0 6 2299.5 7 2420.5 8 2588.5 9 2666.0 10 2739.5 11 2803.0 12 2929.5 13 3066.0 14 3243.0 15 3577.0 16 3973.5 17 4213.0 18 4479.0 19 5127.0 20 5871.0 21 6943.0 22 7653.0 23 8418.0 24 10095.0 25 12498.0 26 13989.0 27 15213.0 28 18888.5 29 22716.5 30 24093.0 31 27042.5 32 33016.0 33 38140.0 34 42230.5 35 44221.0 36 47645.5 37 54103.0 38 57362.5 39 57589.0 40 58822.5 41 62156.0 42 67319.0 43 70382.0 44 70487.5 45 71625.0 46 75036.0 47 77599.0 48 77783.0 49 79430.0 50 81557.0 51 80392.0 52 78747.0 53 78797.0 54 75244.5 55 67969.5 56 60716.0 57 57135.0 58 55986.5 59 49984.0 60 42019.5 61 38909.0 62 34826.0 63 25864.5 64 18498.5 65 16011.0 66 14204.0 67 11455.0 68 9089.5 69 6428.5 70 5191.0 71 4541.0 72 3483.0 73 2797.5 74 2520.0 75 2316.0 76 1591.0 77 847.0 78 624.0 79 532.5 80 390.5 81 286.5 82 189.0 83 145.0 84 125.0 85 91.0 86 73.5 87 70.0 88 62.5 89 53.5 90 47.0 91 42.0 92 40.0 93 32.5 94 31.0 95 25.5 96 16.0 97 21.0 98 22.0 99 48.0 100 78.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00901632468355839 2 0.013581552371436054 3 0.013695683063632997 4 0.01620655829196571 5 0.012725572179958993 6 0.010214696951626277 7 0.016606015714655007 8 0.01883156421249537 9 0.018203845405412193 10-11 0.021085645383384966 12-13 0.016834277099048892 14-15 0.008160344492081327 16-17 0.006334253416930261 18-19 0.017576126598329012 20-21 0.022455213689748267 22-23 0.020001403807514022 24-25 0.018517704808953782 26-27 0.02256934438194521 28-29 0.008788063299164506 30-31 0.00833154053037674 32-33 0.011099209816152573 34-35 0.01244024544946664 36-37 0.01107067714310334 38-39 0.014751391966454707 40-41 0.011926657334580402 42-43 0.014665793947307002 44-45 0.013524487025337584 46-47 0.023111465169880684 48-49 0.0219986909209605 50-51 0.035523177946298085 52-53 0.03800552050158156 54-55 0.028161748299595352 56-57 0.021000047364237263 58-59 0.016691613733802714 60-61 0.02057205726849873 62-63 0.026963376031527462 64-65 0.02579353643650881 66-67 0.027391366127265993 68-69 0.04122971255614517 70-71 0.0530137065254794 72-73 0.08365779738035822 74-75 0.08528415974416464 76-77 0.08907900525971295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1752377.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.6733501304243 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35466761531792 41.51884159139553 2 11.902342161018517 12.776771563609158 3 4.333381030346689 6.9776123187101 4 2.0966980363887924 4.501472312994751 5 1.1336880832150973 3.042441871454676 6 0.7075738756155514 2.2786716221432872 7 0.4600205278880349 1.728359000236198 8 0.3203284559728793 1.3754481099336442 9 0.24323781442345277 1.1749849540658237 >10 1.2756978714544587 12.53975855746755 >50 0.10597252133830255 3.925871275382442 >100 0.06340360594049269 6.002477766276653 >500 0.0022413008098092183 0.7504884337603713 >1k 5.336430499545757E-4 0.3166132030679774 >5k 1.0672860999091515E-4 0.3227426788646616 >10k+ 1.0672860999091515E-4 0.76744474063718 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13397 0.7645044416812136 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5634 0.3215061599187846 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.853267304923541E-4 0.0 2 0.0 0.0 0.0 7.418494992801207E-4 0.0 3 0.0 0.0 0.0 0.0011983722680678872 0.0 4 5.706534609847082E-5 0.0 0.0 0.002853267304923541 0.0 5 5.706534609847082E-5 0.0 0.0 0.005364142533256257 0.0 6 5.706534609847082E-5 0.0 0.0 0.010157631605527805 0.0 7 5.706534609847082E-5 0.0 0.0 0.018888629558593842 0.0 8 5.706534609847082E-5 0.0 0.0 0.026877778012379755 0.0 9 5.706534609847082E-5 0.0 0.0 0.03543757992715038 0.0 10 5.706534609847082E-5 0.0 0.0 0.04622293033976137 0.0 11 5.706534609847082E-5 0.0 0.0 0.058834371827523414 0.0 12 5.706534609847082E-5 0.0 0.0 0.0737284271592243 0.0 13 5.706534609847082E-5 0.0 0.0 0.09644043490641568 0.0 14 5.706534609847082E-5 0.0 0.0 0.1241741931102725 0.0 15 5.706534609847082E-5 0.0 0.0 0.15789981265446876 0.0 16 5.706534609847082E-5 0.0 0.0 0.19425043811919468 0.0 17 5.706534609847082E-5 0.0 0.0 0.23054399823782212 0.0 18 1.1413069219694164E-4 0.0 0.0 0.2661527742032679 0.0 19 1.1413069219694164E-4 0.0 0.0 0.30107676601553207 0.0 20 1.7119603829541245E-4 0.0 0.0 0.3389681558249167 0.0 21 1.7119603829541245E-4 0.0 0.0 0.38068292382289887 0.0 22 2.282613843938833E-4 0.0 0.0 0.4278759650463342 0.0 23 2.282613843938833E-4 0.0 0.0 0.4775228161520038 0.0 24 2.282613843938833E-4 0.0 0.0 0.5264278177583933 0.0 25 2.282613843938833E-4 0.0 0.0 0.5813817460512207 0.0 26 2.282613843938833E-4 0.0 0.0 0.6406155753014334 0.0 27 2.282613843938833E-4 0.0 0.0 0.7001347312821385 0.0 28 2.282613843938833E-4 0.0 0.0 0.7573712734189048 0.0 29 2.853267304923541E-4 0.0 0.0 0.8130099858649138 0.0 30 2.853267304923541E-4 0.0 0.0 0.8738987101519822 0.0 31 2.853267304923541E-4 0.0 0.0 0.9388961393581404 0.0 32 2.853267304923541E-4 0.0 0.0 1.011197932864903 0.0 33 2.853267304923541E-4 0.0 0.0 1.0848692946780287 0.0 34 2.853267304923541E-4 0.0 0.0 1.1632770802173276 0.0 35 2.853267304923541E-4 0.0 0.0 1.248760968672837 0.0 36 3.423920765908249E-4 0.0 0.0 1.3419486788516397 0.0 37 3.423920765908249E-4 0.0 0.0 1.438446179104154 0.0 38 3.423920765908249E-4 0.0 0.0 1.532375738782237 0.0 39 3.423920765908249E-4 0.0 0.0 1.6240797499624795 0.0 40 3.423920765908249E-4 0.0 0.0 1.7223462759440462 0.0 41 3.423920765908249E-4 0.0 0.0 1.829343799878679 0.0 42 3.423920765908249E-4 0.0 0.0 1.9347434941225548 0.0 43 3.423920765908249E-4 0.0 0.0 2.0481894021663147 0.0 44 3.423920765908249E-4 0.0 0.0 2.1742467516978365 0.0 45 3.423920765908249E-4 0.0 0.0 2.3018448655740174 0.0 46 3.9945742268929576E-4 0.0 0.0 2.447703890201709 0.0 47 3.9945742268929576E-4 0.0 0.0 2.5917938891003476 0.0 48 3.9945742268929576E-4 0.0 0.0 2.7390795473805007 0.0 49 4.565227687877666E-4 0.0 0.0 2.882256500741564 0.0 50 4.565227687877666E-4 0.0 0.0 3.032509557018838 0.0 51 4.565227687877666E-4 0.0 0.0 3.1945180745923967 0.0 52 4.565227687877666E-4 0.0 0.0 3.3589804020481893 0.0 53 4.565227687877666E-4 0.0 0.0 3.53285851161023 0.0 54 4.565227687877666E-4 0.0 0.0 3.7199757814671157 0.0 55 4.565227687877666E-4 0.0 0.0 3.916680029468545 0.0 56 4.565227687877666E-4 0.0 0.0 4.1347837822569 0.0 57 4.565227687877666E-4 0.0 0.0 4.345868497475144 0.0 58 4.565227687877666E-4 0.0 0.0 4.5736162937541405 0.0 59 4.565227687877666E-4 0.0 0.0 4.797940169267229 0.0 60 4.565227687877666E-4 0.0 0.0 5.023519482394485 0.0 61 5.135881148862374E-4 0.0 0.0 5.254120545978405 0.0 62 5.135881148862374E-4 0.0 0.0 5.484265086793538 0.0 63 5.135881148862374E-4 0.0 0.0 5.728675964133289 0.0 64 5.135881148862374E-4 0.0 0.0 5.984614041384931 0.0 65 5.135881148862374E-4 0.0 0.0 6.261209773924218 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGGAA 310 0.0 27.482721 59 TCGCGGA 325 0.0 27.30577 58 CGGTTAC 365 0.0 27.225521 4 CGATAAT 70 0.0011308376 25.351742 2 ATTCGCG 355 0.0 24.998955 56 AATTCGC 365 0.0 24.31405 55 CTCGCGC 380 0.0 24.287071 36 TCGCGCT 395 0.0 24.264116 37 CGATCGT 380 0.0 23.35162 1 CAATTCG 375 0.0 22.720997 54 CCTCGCG 455 0.0 22.623491 35 CGCGCTT 435 0.0 22.03356 38 GCCTAGT 455 0.0 21.846487 17 ATCGTTC 420 0.0 21.125849 3 AACTCGA 470 0.0 20.391602 13 ACTCGAG 510 0.0 19.488825 14 ATAACGC 95 0.0066403314 18.686628 51 TAACGCG 95 0.00664252 18.685564 52 CGCTCCG 475 0.0 18.682364 25 GATCGTT 475 0.0 18.680231 2 >>END_MODULE