FastQCFastQC Report
Thu 26 May 2016
SRR936011_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936011_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752377
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106870.6098573537543577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75860.4328977155029996No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59390.33891109047881823No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19730.11258992785228292No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17730.10117685863258877No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14450.053.95585100-101
CGTACAT16050.046.908394100-101
CGGTATC11900.044.262035100-101
TCGTATC5350.043.38563100-101
CCGTATC5550.041.822186100-101
AGGTATC19850.041.226578100-101
CTACGCG1550.040.3145496-97
GGTATCA90300.039.601371
GCGTATC3100.039.35748100-101
CCGTACA7600.039.155384100-101
ACCGTAT2450.038.8677198-99
ACGTATC4600.038.814903100-101
GCGTACA4750.038.215656100-101
AGTATCA29650.037.335617100-101
TCGAGTA3650.036.688057100-101
CGAGTAC11250.035.445194100-101
TACGCAG8450.035.216675100-101
GGGTATC9250.034.101166100-101
TCGGTAT3450.033.639584100-101
ACGTACA6150.032.903255100-101
Produced by FastQC (version 0.11.5)