FastQCFastQC Report
Thu 26 May 2016
SRR936010_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936010_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755606
Sequences flagged as poor quality0
Sequence length77
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132320.7536998620419388No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55780.3177250476473651No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG400.00225287735.51362267
ATTCGCG3500.030.42550756
CGCGGAA3600.029.58372559
CGATCGT3850.026.7375661
TCGCGGA4250.025.05915658
AATTCGC4300.024.76635755
TCGCGCT4200.024.51083437
CAATTCG4350.024.48308454
CGGTTAC3500.024.3410994
CGATCCG901.751413E-423.66900446
CGCGCTT4600.023.15182138
AACTCGA4450.023.132513
ATCGTTC4750.022.4187953
CTCGCGC4700.021.90267236
ACTCGAG4800.021.44331214
CGCTCCG4750.020.92301625
ACGGTAG850.003493848520.88084436
TTCGCGA850.003495579620.8790625
GACGTAT4800.020.70742639
GATCGTT5050.020.3840852
Produced by FastQC (version 0.11.5)