##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936010_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1755606 Sequences flagged as poor quality 0 Sequence length 77 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.89460391454575 40.0 36.0 41.0 31.0 41.0 2 36.76069231934728 40.0 36.0 41.0 30.0 41.0 3 36.62102886410732 39.0 36.0 41.0 30.0 41.0 4 36.51389036036559 39.0 36.0 41.0 30.0 41.0 5 36.425285058264784 39.0 36.0 41.0 30.0 41.0 6 36.306076648177324 39.0 36.0 41.0 30.0 41.0 7 36.1109411792851 39.0 35.0 40.0 30.0 41.0 8 35.948200792205085 39.0 35.0 40.0 29.0 41.0 9 36.069367500452834 39.0 35.0 41.0 30.0 41.0 10-11 36.237836109013074 39.0 35.0 41.0 30.0 41.0 12-13 36.11533424925638 39.0 35.0 41.0 29.5 41.0 14-15 35.92936513090067 39.0 35.0 41.0 28.5 41.0 16-17 35.71989273219617 39.0 35.0 41.0 27.5 41.0 18-19 35.48547709451893 39.0 35.0 41.0 27.0 41.0 20-21 35.154201740025954 39.0 35.0 41.0 25.5 41.0 22-23 34.72347924306479 39.0 34.5 41.0 23.5 41.0 24-25 34.382849568752896 39.0 34.0 41.0 21.5 41.0 26-27 33.38293871176107 38.0 33.0 40.0 18.5 40.5 28-29 33.35694882564767 38.0 33.0 39.5 18.0 40.5 30-31 33.71340579833972 38.0 33.0 40.0 18.0 41.0 32-33 33.56605724746896 38.0 33.0 40.0 16.0 41.0 34-35 33.294142022754535 37.5 33.0 40.0 13.0 41.0 36-37 32.976785224019515 37.0 33.0 40.0 8.5 41.0 38-39 32.66008119133792 36.5 32.0 40.0 7.5 41.0 40-41 32.29091179911666 36.0 32.0 39.5 4.5 41.0 42-43 31.915770964555826 35.5 32.0 39.0 2.0 41.0 44-45 31.559950239404515 35.0 31.0 39.0 2.0 40.5 46-47 31.143493756571807 35.0 31.0 38.0 2.0 40.0 48-49 30.736901389036035 35.0 31.0 37.0 2.0 39.5 50-51 30.292673299134314 35.0 30.0 37.0 2.0 39.0 52-53 29.82846977055216 35.0 30.0 36.0 2.0 39.0 54-55 29.387030746078562 34.0 29.0 36.0 2.0 37.5 56-57 28.97255904798685 34.0 29.0 35.0 2.0 37.0 58-59 28.563381248412227 34.0 29.0 35.0 2.0 37.0 60-61 28.216925950355602 34.0 28.5 35.0 2.0 36.0 62-63 27.854860372999408 34.0 27.5 35.0 2.0 36.0 64-65 27.485994010045534 34.0 27.0 35.0 2.0 35.5 66-67 27.144719544134617 34.0 26.0 35.0 2.0 35.0 68-69 26.784099051837373 33.5 25.0 35.0 2.0 35.0 70-71 26.340582397189344 33.0 24.5 35.0 2.0 35.0 72-73 25.867018567947476 33.0 23.5 35.0 2.0 35.0 74-75 25.32267462061533 33.0 19.0 35.0 2.0 35.0 76-77 23.636923660547982 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6793328649169226 1101 2 0.7236787524812129 1101 3 0.9022900337943796 1101 4 0.6191155101766483 1101 5 0.772959418052487 1101 6 0.9739575589216969 1101 7 1.0870624459651026 1101 8 0.9483247194285269 1101 9 0.9121375780954111 1101 10-11 0.9706736850215876 1101 12-13 1.1245141077380012 1101 14-15 1.1443684655542299 1101 16-17 0.9872785156887574 1101 18-19 1.1451509678215572 1101 20-21 1.0403918149978963 1101 22-23 1.1916006041584026 1101 24-25 1.157122246873648 1101 26-27 1.170263599140526 1101 28-29 1.2187329056170597 1101 30-31 1.2107648298464042 1101 32-33 1.2819313300672377 1101 34-35 1.2234838921205196 1101 36-37 1.1435311571300915 1101 38-39 1.2610802100905758 1101 40-41 1.2817440423387865 1101 42-43 1.176375670555494 1101 44-45 1.1365056485442757 1101 46-47 1.268612152990201 1101 48-49 1.1666354303681175 1101 50-51 1.0729087129919677 1101 52-53 1.07224959503354 1101 54-55 1.0451133816577176 1101 56-57 1.069143979962874 1101 58-59 1.0720814760526025 1101 60-61 0.981287724886414 1101 62-63 0.9353396411773076 1101 64-65 0.9898869521035039 1101 66-67 0.9481635098416703 1101 68-69 0.8335603027034928 1101 70-71 0.8399756797181333 1101 72-73 0.9495428076239492 1101 74-75 1.1108395098609058 1101 76-77 1.4573008778785557 1102 1 0.751841589366876 1102 2 0.8810920310387971 1102 3 0.889654328544097 1102 4 0.7260750745654221 1102 5 0.7169486453928897 1102 6 0.8438198817372182 1102 7 0.7997003967075571 1102 8 0.9300047111309055 1102 9 0.8697630021993845 1102 10-11 0.9700805899456668 1102 12-13 1.0636316863418145 1102 14-15 1.0314041699490915 1102 16-17 1.1080266495243691 1102 18-19 1.0692781040241996 1102 20-21 1.104208702596047 1102 22-23 1.1085061662425204 1102 24-25 1.164685278373625 1102 26-27 1.0787833172350219 1102 28-29 1.1722029217026275 1102 30-31 1.3277029337456838 1102 32-33 1.2857757552408486 1102 34-35 1.3241186535512313 1102 36-37 1.2597498124652162 1102 38-39 1.308862857853768 1102 40-41 1.3449359346150374 1102 42-43 1.411255043072309 1102 44-45 1.3094317137540834 1102 46-47 1.5239338803368412 1102 48-49 1.2090763017473982 1102 50-51 1.1747957818441037 1102 52-53 1.2363590738262928 1102 54-55 1.2470054048497303 1102 56-57 1.2892633403469063 1102 58-59 1.1915142148295104 1102 60-61 1.0292983079266733 1102 62-63 0.9679349204399017 1102 64-65 0.9853768988924685 1102 66-67 1.0498026834862912 1102 68-69 1.2681619470453356 1102 70-71 1.2398605893335137 1102 72-73 1.4304980043522306 1102 74-75 1.2835276598284189 1102 76-77 1.1006719889445158 1103 1 0.7666296916170623 1103 2 0.7850007231173564 1103 3 0.7131724877548749 1103 4 0.7697859706524568 1103 5 0.6127109006091374 1103 6 0.5914684792341447 1103 7 0.4627559621898172 1103 8 0.6733604180862045 1103 9 0.6727423446124945 1103 10-11 0.6662018881903222 1103 12-13 0.6920971277496264 1103 14-15 0.6583531030153154 1103 16-17 0.7136069517679644 1103 18-19 0.7031085199577447 1103 20-21 0.7245291085585137 1103 22-23 0.46956303083830875 1103 24-25 0.6355025216883448 1103 26-27 0.6358980659574556 1103 28-29 0.5842745172929327 1103 30-31 0.7452905065405346 1103 32-33 0.6037535876271676 1103 34-35 0.6901657888096935 1103 36-37 0.650370443985743 1103 38-39 0.8403712660052491 1103 40-41 0.7245629755160934 1103 42-43 0.8688534207920213 1103 44-45 0.8064400388716031 1103 46-47 0.7664179059351284 1103 48-49 0.7346476068940291 1103 50-51 0.6658249614475977 1103 52-53 0.7541145013390498 1103 54-55 0.6955415197154693 1103 56-57 0.8123233062784578 1103 58-59 0.6894981013371577 1103 60-61 0.5634555693336836 1103 62-63 0.3964451371199864 1103 64-65 0.30336559226033444 1103 66-67 0.21461737762353295 1103 68-69 0.2405053334433198 1103 70-71 0.10732333091151958 1103 72-73 0.2031112686237364 1103 74-75 0.010787041789519947 1103 76-77 0.1343818479829899 1104 1 0.5941150165066702 1104 2 0.49990761323515187 1104 3 0.8507522379238566 1104 4 0.79199324756447 1104 5 0.7809711091029641 1104 6 0.7257341031168991 1104 7 0.7629343165537605 1104 8 0.8155946505216036 1104 9 0.6135885969447941 1104 10-11 0.7771276444762378 1104 12-13 0.785839075158016 1104 14-15 0.9014813333271903 1104 16-17 0.9047051532490329 1104 18-19 0.8275595946035068 1104 20-21 0.8490088521167749 1104 22-23 0.7217948213569301 1104 24-25 0.7093812973841196 1104 26-27 0.7367880164580498 1104 28-29 0.7479200427165225 1104 30-31 0.9376963477134126 1104 32-33 1.013396595582961 1104 34-35 1.0939991259655457 1104 36-37 1.1071518897353982 1104 38-39 0.9182377187905857 1104 40-41 1.0339862109613875 1104 42-43 1.1439555833049582 1104 44-45 0.9744496473419524 1104 46-47 0.9979524280277303 1104 48-49 0.8354012957398673 1104 50-51 0.8138901134063303 1104 52-53 0.7973644227525014 1104 54-55 0.8288435308565525 1104 56-57 0.7601846728614348 1104 58-59 0.6947738558017491 1104 60-61 0.7568256978108892 1104 62-63 0.761358233214132 1104 64-65 0.5615901973376296 1104 66-67 0.6562538800971538 1104 68-69 0.4864822418147803 1104 70-71 0.5871603161021746 1104 72-73 0.4984476123053092 1104 74-75 0.4697321472304061 1104 76-77 0.4930215114571759 1105 1 0.031489153777869205 1105 2 0.06804636245984597 1105 3 -0.02913743836314353 1105 4 0.07743012154998041 1105 5 -0.0015110424733748573 1105 6 0.10051800852355086 1105 7 0.30305203037194417 1105 8 0.08950032110766415 1105 9 0.06211387506348842 1105 10-11 0.16023696524639064 1105 12-13 0.2986968684198317 1105 14-15 0.3812139698791768 1105 16-17 0.21581868373981905 1105 18-19 0.14809649894123567 1105 20-21 0.014012745118613168 1105 22-23 0.1493724504528302 1105 24-25 0.162405961860145 1105 26-27 0.22290253669505944 1105 28-29 0.30011760926046094 1105 30-31 0.24338672869972555 1105 32-33 0.29600543467661566 1105 34-35 0.3123087713724644 1105 36-37 0.30785757879672104 1105 38-39 0.24366792848481822 1105 40-41 0.2746575228370318 1105 42-43 0.38461006949469834 1105 44-45 0.10070101649090546 1105 46-47 0.027188871803545567 1105 48-49 0.022327077969304554 1105 50-51 -0.025880199485449396 1105 52-53 0.06537846395233515 1105 54-55 0.00960847272545351 1105 56-57 0.05073754510499384 1105 58-59 0.1867859439023576 1105 60-61 0.06432236566524807 1105 62-63 -0.07638837855461134 1105 64-65 -0.022504580761264492 1105 66-67 -0.04909569827094984 1105 68-69 -0.08901065180729617 1105 70-71 -0.2352874582884752 1105 72-73 -0.6384392399057823 1105 74-75 -0.8341938461448919 1105 76-77 -0.6648205778311258 1106 1 0.15916794959284886 1106 2 0.2744316910843736 1106 3 0.2615600207323894 1106 4 0.18343186751244644 1106 5 0.3597755466831387 1106 6 0.20171108287245687 1106 7 -0.17227302286669044 1106 8 -0.0692202323095259 1106 9 0.2682882332238776 1106 10-11 0.008554823003350975 1106 12-13 0.4367280973393193 1106 14-15 0.25071584149802817 1106 16-17 0.2019865606759197 1106 18-19 0.30049885069976057 1106 20-21 0.3482686608891541 1106 22-23 0.23731274382743806 1106 24-25 0.11733632176649422 1106 26-27 0.0012750880488425764 1106 28-29 0.3484076903418938 1106 30-31 0.44650102310603046 1106 32-33 0.32264789595461707 1106 34-35 0.10333029168250363 1106 36-37 0.31282265954748567 1106 38-39 0.5033914182934254 1106 40-41 0.3521888377319371 1106 42-43 0.5599380988057021 1106 44-45 0.3066445637046371 1106 46-47 0.3962509460598582 1106 48-49 0.37510309214863113 1106 50-51 0.27942481486583404 1106 52-53 0.3426399482849689 1106 54-55 0.38584122803939636 1106 56-57 0.42107304766441445 1106 58-59 0.26566112081332705 1106 60-61 0.11533456094850436 1106 62-63 0.14147433853333524 1106 64-65 0.14176866236400798 1106 66-67 0.10756772778357515 1106 68-69 0.14372316827920883 1106 70-71 0.01632127416923268 1106 72-73 0.11503780465594104 1106 74-75 0.2239539786768745 1106 76-77 0.17237106800303437 1107 1 -0.11647916975267236 1107 2 -0.05951101269872794 1107 3 0.0264539966885593 1107 4 -0.015233162192764382 1107 5 -0.16412781101217888 1107 6 -0.21474743567742394 1107 7 -0.13947670526024325 1107 8 -0.21143574931866027 1107 9 -0.19147471525673865 1107 10-11 -0.012095137932789157 1107 12-13 -0.12006202136604571 1107 14-15 -0.37839853156240366 1107 16-17 -0.24650784530334846 1107 18-19 -0.1251101669565884 1107 20-21 -0.11519188333639363 1107 22-23 -0.1166176314292997 1107 24-25 0.128647840920614 1107 26-27 -0.19455027536044867 1107 28-29 -0.15866742130118183 1107 30-31 0.0531184013249586 1107 32-33 0.1283777100773804 1107 34-35 -0.05166885519886222 1107 36-37 -0.05891576530046194 1107 38-39 -8.038966380041757E-5 1107 40-41 -0.03846091794258655 1107 42-43 -0.08553658794581409 1107 44-45 -0.03954808972521917 1107 46-47 -0.2008528765530464 1107 48-49 -0.14928230964458322 1107 50-51 -0.08538025714891972 1107 52-53 -0.0638146318074746 1107 54-55 0.02348903244559608 1107 56-57 0.0995871587597037 1107 58-59 -0.007637997972857136 1107 60-61 -0.08773910360011783 1107 62-63 -0.0568375770322902 1107 64-65 -0.10315464034685817 1107 66-67 -0.1271826163503711 1107 68-69 -0.16608269270992082 1107 70-71 -0.13494820483827752 1107 72-73 -0.09142909113401743 1107 74-75 -0.1397372282999676 1107 76-77 0.06703272628604395 1108 1 0.3265540182670108 1108 2 0.36707924019360405 1108 3 0.47241766643739425 1108 4 0.4756566954537931 1108 5 0.4376392421973705 1108 6 0.49386284242875433 1108 7 0.5361692540580663 1108 8 0.6113940608787942 1108 9 0.6849418783240893 1108 10-11 0.5788080191916549 1108 12-13 0.6618184206178981 1108 14-15 0.6407395396946001 1108 16-17 0.7040479748117079 1108 18-19 0.8026738213300675 1108 20-21 0.6801738921426832 1108 22-23 0.6261788933006756 1108 24-25 0.7079396958177995 1108 26-27 0.43081676264970525 1108 28-29 0.5673455607052986 1108 30-31 0.5809351480030003 1108 32-33 0.6661090030133963 1108 34-35 0.6170540210675739 1108 36-37 0.7642304437661025 1108 38-39 0.7656430253234277 1108 40-41 0.662039436608012 1108 42-43 0.5253772271473167 1108 44-45 0.48993660760134006 1108 46-47 0.5057592622542799 1108 48-49 0.5637365615177963 1108 50-51 0.46999965745249384 1108 52-53 0.5589033892302133 1108 54-55 0.6870384424574638 1108 56-57 0.7169538253650813 1108 58-59 0.6760054215741889 1108 60-61 0.8287323610523813 1108 62-63 0.7130600047303837 1108 64-65 0.7951026683904097 1108 66-67 0.6592050922353927 1108 68-69 0.6100495555821404 1108 70-71 0.8352506989660462 1108 72-73 0.989679184389491 1108 74-75 0.8420533655983284 1108 76-77 0.8593445248841434 1109 1 -0.43295979058111556 1109 2 -0.3527974364199338 1109 3 -0.3685495504889005 1109 4 -0.22244623897105242 1109 5 -0.22240286928724373 1109 6 -0.5039654640258888 1109 7 -0.12987760870462495 1109 8 -0.25675999029507324 1109 9 -0.21068738664332187 1109 10-11 -0.37369006474511224 1109 12-13 -0.2769232760533882 1109 14-15 -0.26630772381943046 1109 16-17 -0.19348478141072434 1109 18-19 -0.40628913291816815 1109 20-21 -0.30488624694380206 1109 22-23 -0.4068238020702566 1109 24-25 -0.21400226259905253 1109 26-27 -0.32386661119852533 1109 28-29 -0.11526155180835218 1109 30-31 -0.11979564115430463 1109 32-33 -0.10601108952880622 1109 34-35 0.07708644746948323 1109 36-37 0.08157055996847618 1109 38-39 0.14403998271744456 1109 40-41 0.1677314404236867 1109 42-43 0.05589082522072886 1109 44-45 -0.027849643244163502 1109 46-47 -0.01496991893095867 1109 48-49 0.1814557928326508 1109 50-51 -0.031025191924292983 1109 52-53 3.603236718277003E-4 1109 54-55 -0.00497887645709838 1109 56-57 0.0014896041404099947 1109 58-59 0.13762253859202644 1109 60-61 0.1234880750183649 1109 62-63 0.09469929189587845 1109 64-65 0.14485459679281476 1109 66-67 0.022131837311910374 1109 68-69 -0.04305716751047939 1109 70-71 -0.10574914303051841 1109 72-73 -0.0802337647212994 1109 74-75 -0.0955965863332402 1109 76-77 -0.027669672764179865 1110 1 -0.22916037463900096 1110 2 -0.07063564237786579 1110 3 -0.4114066626417099 1110 4 -0.3575542215150236 1110 5 -0.39376039088237036 1110 6 -0.20793298620746725 1110 7 -0.3172907346240308 1110 8 -0.6653186397218676 1110 9 -0.3992163081320257 1110 10-11 -0.2586774560095435 1110 12-13 -0.11754274831340439 1110 14-15 -0.07303590355278544 1110 16-17 -0.1689059604633698 1110 18-19 -0.4370458197852187 1110 20-21 -0.3528283041772937 1110 22-23 -0.3618246019182081 1110 24-25 -0.4084824520079593 1110 26-27 -0.4548674282409024 1110 28-29 -0.3525164122279776 1110 30-31 -0.16478394126708196 1110 32-33 -0.13783229572408828 1110 34-35 -0.3122819919734283 1110 36-37 -0.24551428747242454 1110 38-39 -0.1796716863733323 1110 40-41 -0.2084020485192184 1110 42-43 -0.273163551851237 1110 44-45 -0.3380044268722422 1110 46-47 -0.44387111207075236 1110 48-49 -0.3408720932444389 1110 50-51 -0.3337625755894038 1110 52-53 -0.3038889414966519 1110 54-55 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2314 6 -0.36762248568926736 2314 7 -0.39283658579782355 2314 8 -0.44326459310023836 2314 9 -0.24725602933224877 2314 10-11 -0.4927648999235714 2314 12-13 -0.39061030104172545 2314 14-15 -0.3987148561508107 2314 16-17 -0.508217692795661 2314 18-19 -0.4835491702600123 2314 20-21 -0.3370311207035783 2314 22-23 -0.25257424916898685 2314 24-25 -0.17120571662116646 2314 26-27 -0.19740407207123667 2314 28-29 -0.3554760411517677 2314 30-31 -0.4234627360651544 2314 32-33 -0.5520375672171554 2314 34-35 -0.48397376182584395 2314 36-37 -1.114562650859483 2314 38-39 -0.5982796579509326 2314 40-41 -0.43517209445985117 2314 42-43 -0.40476547046056055 2314 44-45 -0.4014490781979383 2314 46-47 -0.3116959167628508 2314 48-49 -0.30023332550243254 2314 50-51 -0.2117465308792319 2314 52-53 -0.21407491538089118 2314 54-55 -0.30947819474220495 2314 56-57 -0.2925911825172598 2314 58-59 -0.2779677792858237 2314 60-61 -0.36184079093465016 2314 62-63 -0.38565000582957154 2314 64-65 -0.4313784414914288 2314 66-67 -0.36681552800267525 2314 68-69 -0.24933445332117188 2314 70-71 -0.15057312680314894 2314 72-73 -0.19122540904122687 2314 74-75 -0.0729662095898469 2314 76-77 -0.20551561110095307 2315 1 -0.9505852291126544 2315 2 -0.8433042122527965 2315 3 -0.7635695563205616 2315 4 -0.5744600706490033 2315 5 -0.8975693145668373 2315 6 -0.8306283547702122 2315 7 -1.1553578528012238 2315 8 -1.127318267548823 2315 9 -0.7094484482830339 2315 10-11 -0.8445797862139415 2315 12-13 -0.8148877839261317 2315 14-15 -0.5896317291975137 2315 16-17 -0.7817405540593185 2315 18-19 -0.7162889824983765 2315 20-21 -0.7335788791512243 2315 22-23 -0.8457937215841014 2315 24-25 -0.9963595836457912 2315 26-27 -0.8373015569548343 2315 28-29 -0.9247210472309675 2315 30-31 -1.0633961759545016 2315 32-33 -0.7635680060620302 2315 34-35 -0.9570624600200333 2315 36-37 -0.9955271018137921 2315 38-39 -0.8438520922425212 2315 40-41 -0.9166534814968728 2315 42-43 -0.8939924837368842 2315 44-45 -0.8662906925725977 2315 46-47 -0.7287372634894638 2315 48-49 -0.7746603914107624 2315 50-51 -0.574923326487518 2315 52-53 -0.5725973227444783 2315 54-55 -0.8129366327955552 2315 56-57 -0.9465313921476906 2315 58-59 -0.6421157425494961 2315 60-61 -0.7903777945005714 2315 62-63 -0.7587206755208804 2315 64-65 -0.5615573648200538 2315 66-67 -0.5766518740764894 2315 68-69 -0.9071900610780688 2315 70-71 -0.8568927673142888 2315 72-73 -0.8651651443715842 2315 74-75 -0.9215183703581964 2315 76-77 -0.8657132667325804 2316 1 -2.0066818280712724 2316 2 -1.3554394535547019 2316 3 -1.731087978244723 2316 4 -2.5448211265633347 2316 5 -2.1105110037721886 2316 6 -2.0680256657672444 2316 7 -1.71972361884049 2316 8 -1.5788595225395312 2316 9 -1.8334819297277178 2316 10-11 -1.8183854659622511 2316 12-13 -2.3925847214249742 2316 14-15 -3.5357777867677456 2316 16-17 -2.861428887760624 2316 18-19 -2.4141263037988168 2316 20-21 -2.104490292924659 2316 22-23 -2.347061235061311 2316 24-25 -2.3432328012498758 2316 26-27 -2.356245263142391 2316 28-29 -2.8368023566824974 2316 30-31 -2.94391154135716 2316 32-33 -3.040636850241526 2316 34-35 -3.0031813791751603 2316 36-37 -2.9520837889032663 2316 38-39 -2.980455489604118 2316 40-41 -2.7439945147805282 2316 42-43 -2.9751668181557527 2316 44-45 -2.8586298399663868 2316 46-47 -2.821592040770472 2316 48-49 -2.6082110620515984 2316 50-51 -2.693120611266089 2316 52-53 -2.813403609657321 2316 54-55 -2.7004044921725665 2316 56-57 -2.387817546036306 2316 58-59 -2.7676395369599014 2316 60-61 -2.55343470697688 2316 62-63 -2.79022308578012 2316 64-65 -2.7518226658264773 2316 66-67 -2.3983671484044677 2316 68-69 -2.303555525834927 2316 70-71 -2.1408517216113587 2316 72-73 -2.2514612221806374 2316 74-75 -2.4303101819164006 2316 76-77 -2.6122508189395326 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39762.0 3 4227.0 4 6336.0 5 7883.0 6 8650.0 7 8688.0 8 11099.0 9 12142.0 10 17205.0 11 19418.0 12 13010.0 13 9433.0 14 8845.0 15 8939.0 16 8780.0 17 9028.0 18 9610.0 19 10168.0 20 10771.0 21 11578.0 22 12915.0 23 14781.0 24 16714.0 25 19554.0 26 23057.0 27 27299.0 28 31941.0 29 38029.0 30 45441.0 31 53598.0 32 66759.0 33 85341.0 34 116780.0 35 156774.0 36 216224.0 37 284039.0 38 245299.0 39 64984.0 40 505.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 22.543430635729493 25.720141402334757 29.968819030758308 21.76760893117744 2 22.504977313703208 26.06050248794479 29.77159897804855 21.662921220303453 3 22.376274285844495 26.10324172052444 29.500254928377945 22.02022906525312 4 22.103720719304125 26.003901434096072 29.884654739034623 22.00772310756518 5 22.375954361550495 26.064196444949815 29.607397220630276 21.95245197286941 6 22.42585755375929 25.797281600299883 29.842523318432402 21.93433752750843 7 22.32609307471523 25.780702543984486 29.772605820844333 22.12059856045595 8 22.252721114595886 26.0697163659981 29.410199657599605 22.267362861806404 9 22.15538261701837 26.01408322318946 29.64507087188369 22.185463287908483 10-11 22.62878831687619 26.032985693209753 29.2721321114131 22.066093878500958 12-13 22.835765156031947 25.777595972273776 29.037646558136 22.34899231355828 14-15 22.47044374542144 26.005516483942152 29.054849253702436 22.469190516933963 16-17 22.50548914716782 25.94474709478929 29.228266779530387 22.321496978512506 18-19 22.633334254286062 26.060873456252985 28.754517771586823 22.551274517874127 20-21 22.50931537610991 26.193396379578576 28.673841352921304 22.623446891390206 22-23 22.798632770775477 26.440333262123477 27.99552802107812 22.76550594602293 24-25 22.910774798261667 26.60160068913496 27.602914466592676 22.8847100460107 26-27 22.889161954014543 26.639544349631223 27.403538035332502 23.06775566102174 28-29 23.084247427472498 26.743633621043227 27.30376787553433 22.868351075949946 30-31 22.842456555193483 26.614912378391143 27.261461217465705 23.28116984894967 32-33 22.83167425119137 26.893138096264835 27.20377273256796 23.071414919975837 34-35 23.00743446033725 26.356715666713093 27.427806607757233 23.208043265192423 36-37 23.011123393533662 26.609015551331872 27.604489916941265 22.775371138193204 38-39 22.912292425616183 26.65819520202905 27.601421861563054 22.828090510791718 40-41 22.72331090145831 26.56949542238216 27.615417437388523 23.091776238771008 42-43 22.812867802465366 26.844324633493567 27.219233911259128 23.12357365278194 44-45 22.774138048902763 26.600534375498487 27.59468359045644 23.03064398514231 46-47 22.753631362254342 26.680664010760353 27.490054593629814 23.075650033355494 48-49 22.82159520106643 26.69557762092754 27.291826887472297 23.19100029053373 50-51 22.59298740189063 27.102258224778712 27.076819296438437 23.227935076892223 52-53 22.54462816768756 26.89877501694362 27.324908102767644 23.23168871260118 54-55 22.555530581879992 26.81950556110579 27.319055037897787 23.30590881911643 56-57 22.482605236644833 27.201288182385024 27.15762146568061 23.158485115289533 58-59 22.602806255353343 26.974352663903083 27.034911129170407 23.387929951573167 60-61 22.517854187455367 26.662139323479334 27.037924000454634 23.782082488610666 62-63 22.596559377361764 26.604219005894848 27.0340333294116 23.76518828733179 64-65 22.4774328368923 26.571611450266214 27.157108588725148 23.793847124116336 66-67 22.431528359873877 26.65349870575504 27.280410626168443 23.63456230820264 68-69 22.452301063800086 26.347726056806277 27.342186274124376 23.85778660526926 70-71 22.3469932376307 26.209531147904425 27.469165265854212 23.97431034861067 72-73 22.610294327199192 26.1329243143974 27.305262869903935 23.951518488499477 74-75 22.358438455009214 26.140624100392362 27.19147982855224 24.309457616046178 76-77 22.34046858736335 25.932397876296676 27.615154586403417 24.111978949936557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 14605.0 1 8485.5 2 2243.0 3 2083.0 4 2046.0 5 2176.5 6 2283.0 7 2387.5 8 2521.5 9 2527.0 10 2622.5 11 2783.5 12 2943.5 13 3038.0 14 3238.5 15 3595.5 16 4000.5 17 4249.0 18 4507.5 19 5112.0 20 5830.5 21 6909.0 22 7615.0 23 8477.0 24 10140.5 25 12524.5 26 14107.0 27 15201.5 28 18900.0 29 22832.5 30 24161.0 31 27231.5 32 32902.5 33 37857.5 34 42204.0 35 44196.0 36 47774.5 37 54378.0 38 57728.0 39 58053.0 40 59002.5 41 62115.0 42 67401.5 43 70525.0 44 70800.5 45 71894.5 46 74977.0 47 77785.5 48 78330.0 49 79772.5 50 81847.0 51 80524.5 52 78570.0 53 78700.5 54 75097.5 55 67789.5 56 60978.0 57 57741.0 58 56565.0 59 50189.5 60 41966.5 61 38943.0 62 34941.5 63 26123.5 64 18725.5 65 16144.0 66 14281.5 67 11514.5 68 9107.5 69 6500.5 70 5396.0 71 4741.0 72 3555.5 73 2816.0 74 2607.0 75 2291.0 76 1534.0 77 864.0 78 635.0 79 522.5 80 390.0 81 290.0 82 175.0 83 140.0 84 127.5 85 102.0 86 78.0 87 67.0 88 56.0 89 45.5 90 45.5 91 45.0 92 40.0 93 30.0 94 17.5 95 16.5 96 23.0 97 18.0 98 15.5 99 32.5 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0027340986531146512 2 0.008031414793524287 3 0.012018641995983154 4 0.02016397756672055 5 0.013898334819999476 6 0.013385691322540479 7 0.018683007462950114 8 0.019993096400900887 9 0.018569086685737007 10-11 0.022243031750859815 12-13 0.021217744755941823 14-15 0.007974454404917732 16-17 0.00549667750053258 18-19 0.010110468977663553 20-21 0.009968068006147164 22-23 0.013157849768114259 24-25 0.02061966067557299 26-27 0.012816087436474928 28-29 0.007632692073278401 30-31 0.007490291101762013 32-33 0.009540865091598 34-35 0.011363597527007769 36-37 0.00919910275995867 38-39 0.0171735571648764 40-41 0.012360404327622483 42-43 0.014126176374425696 44-45 0.016290671141474797 46-47 0.015550186089589578 48-49 0.01230344393901593 50-51 0.024037283991966307 52-53 0.029647882269712 54-55 0.024179684963482693 56-57 0.015066022786433859 58-59 0.015806507838319078 60-61 0.01967981426356483 62-63 0.02446448690651547 64-65 0.01967981426356483 66-67 0.019110210377499277 68-69 0.027511867696966178 70-71 0.05522309675405529 72-73 0.06630189233803029 74-75 0.07797877200237412 76-77 0.07473202985180045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1755606.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.49382168715982 #Duplication Level Percentage of deduplicated Percentage of total 1 77.04908005552272 41.21649749649835 2 12.091396262467637 12.93629991226469 3 4.41941805443266 7.092346840945065 4 2.0807891062758923 4.452374456788291 5 1.1828652566229518 3.163799155886237 6 0.7091639449581744 2.276153377113324 7 0.46012539920267986 1.7229706241076976 8 0.3151124275430143 1.3485254408315261 9 0.24510570602752574 1.1800476839467688 >10 1.2731307190357408 12.4807998573596 >50 0.10769676420262402 3.996277122413875 >100 0.06291744900570391 5.926359329024003 >500 0.0024524552719778314 0.8125298762771719 >1k 5.331424504299633E-4 0.3221029281387988 >5k 1.0662849008599266E-4 0.31816719198835125 >10k+ 1.0662849008599266E-4 0.7547487064162539 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13232 0.7536998620419388 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5578 0.3177250476473651 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.8480194303277616E-4 0.0 2 0.0 0.0 0.0 9.683266063114389E-4 0.0 3 0.0 0.0 0.0 0.0015948908809835464 0.0 4 0.0 0.0 0.0 0.0028480194303277615 0.0 5 5.696038860655523E-5 0.0 0.0 0.005923880415081744 0.0 6 5.696038860655523E-5 0.0 0.0 0.00991110761754061 0.0 7 5.696038860655523E-5 0.0 0.0 0.018796928240163227 0.0 8 5.696038860655523E-5 0.0 0.0 0.026087857981802295 0.0 9 5.696038860655523E-5 0.0 0.0 0.035258480547457685 0.0 10 5.696038860655523E-5 0.0 0.0 0.045226548553604855 0.0 11 5.696038860655523E-5 0.0 0.0 0.05565029966860446 0.0 12 1.7088116581966568E-4 0.0 0.0 0.07233969353032514 0.0 13 1.7088116581966568E-4 0.0 0.0 0.0952947301387669 0.0 14 2.2784155442622092E-4 0.0 0.0 0.1213825881205692 0.0 15 2.2784155442622092E-4 0.0 0.0 0.15743851410851864 0.0 16 2.2784155442622092E-4 0.0 0.0 0.19446276670277957 0.0 17 2.2784155442622092E-4 0.0 0.0 0.22892380180974548 0.0 18 2.8480194303277616E-4 0.0 0.0 0.26475188624326873 0.0 19 2.8480194303277616E-4 0.0 0.0 0.2982445947439232 0.0 20 2.8480194303277616E-4 0.0 0.0 0.3361232531672824 0.0 21 2.8480194303277616E-4 0.0 0.0 0.3778752180158874 0.0 22 2.8480194303277616E-4 0.0 0.0 0.4225321626834267 0.0 23 2.8480194303277616E-4 0.0 0.0 0.4698662456154741 0.0 24 3.4176233163933135E-4 0.0 0.0 0.5213014765271935 0.0 25 3.4176233163933135E-4 0.0 0.0 0.5743315983198963 0.0 26 3.4176233163933135E-4 0.0 0.0 0.6355640160719432 0.0 27 3.4176233163933135E-4 0.0 0.0 0.6926383254557116 0.0 28 3.4176233163933135E-4 0.0 0.0 0.7498835160052996 0.0 29 3.4176233163933135E-4 0.0 0.0 0.804508528678986 0.0 30 3.4176233163933135E-4 0.0 0.0 0.8643169367158691 0.0 31 3.4176233163933135E-4 0.0 0.0 0.9273151265147191 0.0 32 3.987227202458866E-4 0.0 0.0 0.9976042460552083 0.0 33 3.987227202458866E-4 0.0 0.0 1.066298474714714 0.0 34 3.987227202458866E-4 0.0 0.0 1.1447329298259405 0.0 35 3.987227202458866E-4 0.0 0.0 1.227268532916839 0.0 36 3.987227202458866E-4 0.0 0.0 1.3152723333139669 0.0 37 3.987227202458866E-4 0.0 0.0 1.406295034307242 0.0 38 3.987227202458866E-4 0.0 0.0 1.4994252696789598 0.0 39 3.987227202458866E-4 0.0 0.0 1.58970748562035 0.0 40 3.987227202458866E-4 0.0 0.0 1.6834642852667399 0.0 41 3.987227202458866E-4 0.0 0.0 1.7888979645774736 0.0 42 4.5568310885244184E-4 0.0 0.0 1.893989881556568 0.0 43 4.5568310885244184E-4 0.0 0.0 2.008081539935498 0.0 44 5.126434974589971E-4 0.0 0.0 2.1382929882900834 0.0 45 5.126434974589971E-4 0.0 0.0 2.26417544711057 0.0 46 5.696038860655523E-4 0.0 0.0 2.408000428342122 0.0 47 5.696038860655523E-4 0.0 0.0 2.5509140433559696 0.0 48 5.696038860655523E-4 0.0 0.0 2.702884360158259 0.0 49 5.696038860655523E-4 0.0 0.0 2.8513231328669417 0.0 50 5.696038860655523E-4 0.0 0.0 3.0030656081148046 0.0 51 5.696038860655523E-4 0.0 0.0 3.1620420527157003 0.0 52 5.696038860655523E-4 0.0 0.0 3.3294486348303662 0.0 53 5.696038860655523E-4 0.0 0.0 3.50465879018413 0.0 54 5.696038860655523E-4 0.0 0.0 3.687159875279533 0.0 55 5.696038860655523E-4 0.0 0.0 3.8832744932519025 0.0 56 6.265642746721076E-4 0.0 0.0 4.096648108972059 0.0 57 6.265642746721076E-4 0.0 0.0 4.309281239640329 0.0 58 6.265642746721076E-4 0.0 0.0 4.529717943547698 0.0 59 6.265642746721076E-4 0.0 0.0 4.75192041950187 0.0 60 6.835246632786627E-4 0.0 0.0 4.969395183201698 0.0 61 6.835246632786627E-4 0.0 0.0 5.200426519389886 0.0 62 6.835246632786627E-4 0.0 0.0 5.438407022988073 0.0 63 6.835246632786627E-4 0.0 0.0 5.680146912234294 0.0 64 6.835246632786627E-4 0.0 0.0 5.936924344072645 0.0 65 6.835246632786627E-4 0.0 0.0 6.215517604747307 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGACGG 40 0.002252877 35.513622 67 ATTCGCG 350 0.0 30.425507 56 CGCGGAA 360 0.0 29.583725 59 CGATCGT 385 0.0 26.737566 1 TCGCGGA 425 0.0 25.059156 58 AATTCGC 430 0.0 24.766357 55 TCGCGCT 420 0.0 24.510834 37 CAATTCG 435 0.0 24.483084 54 CGGTTAC 350 0.0 24.341099 4 CGATCCG 90 1.751413E-4 23.669004 46 CGCGCTT 460 0.0 23.151821 38 AACTCGA 445 0.0 23.1325 13 ATCGTTC 475 0.0 22.418795 3 CTCGCGC 470 0.0 21.902672 36 ACTCGAG 480 0.0 21.443312 14 CGCTCCG 475 0.0 20.923016 25 ACGGTAG 85 0.0034938485 20.880844 36 TTCGCGA 85 0.0034955796 20.87906 25 GACGTAT 480 0.0 20.707426 39 GATCGTT 505 0.0 20.384085 2 >>END_MODULE