FastQCFastQC Report
Thu 26 May 2016
SRR936010_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936010_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755606
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106830.6085078314838295No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73990.42144991529990217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57610.3281487987623647No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19660.11198412400048759No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18640.10617416436261894No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA14000.050.164097100-101
CGTACAT16000.045.009525100-101
CGGTATC11700.044.256023100-101
TACGCAG8700.038.651665100-101
AGGTATC18750.038.566837100-101
ACGTATC4400.038.550602100-101
CCGTATC6000.038.189896100-101
GGTATCA87800.038.166091
AGTATCA30400.037.88318100-101
TCGAGTA2750.037.874275100-101
CTACGCG1650.037.86996596-97
CCGTACA7400.036.996883100-101
ACCGTAT2500.036.8977498-99
TCGGTAT3450.036.22757100-101
GGGTATC10050.036.12458100-101
TCGTATC4700.036.089928100-101
TGTATCA24350.035.196747100-101
GCGTATC3650.035.0578100-101
GCGTACA4700.034.823616100-101
GACGCGT952.910383E-1134.4531296-97
Produced by FastQC (version 0.11.5)