FastQCFastQC Report
Thu 26 May 2016
SRR936009_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936009_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286990
Sequences flagged as poor quality0
Sequence length77
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44150.1343174150210375No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA950.00665356918.6807732
TAAGGCG14400.018.24219517
CGGTCGA1200.001215862917.75916367
CGCGGAA2451.1174052E-817.387259
GACGCGA10250.016.63398669
CTCGACG1502.8419925E-416.56311230
CCGGGGT11850.016.47827348
GTAACGT2602.3295797E-816.3823472
GGTAACG2854.110916E-916.1909891
CGGGGTG11650.016.1519149
GGACGCG11600.015.616528568
ATTACCG3101.2503733E-814.88458253
GGCGGAA17550.014.76613520
CGCGACC12800.014.70837471
AGGCGGA19150.014.45928919
TCGCGGA3001.3470344E-714.19954658
TACCGCG2303.272904E-513.8889585
TGGACGC13100.013.82774567
GCGGAAG19300.013.79510621
ACGCGAC12900.013.7682670
Produced by FastQC (version 0.11.5)