##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936009_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3286990 Sequences flagged as poor quality 0 Sequence length 77 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.73009257709941 40.0 36.0 41.0 30.0 41.0 2 36.58545234393777 39.0 36.0 41.0 30.0 41.0 3 36.49817675137436 39.0 36.0 41.0 30.0 41.0 4 36.391119839123334 39.0 36.0 41.0 30.0 41.0 5 36.30860939643869 39.0 36.0 41.0 30.0 41.0 6 36.2143012300007 39.0 36.0 41.0 29.0 41.0 7 36.05481975911092 39.0 35.0 40.0 29.0 41.0 8 35.876211670859966 39.0 35.0 40.0 28.0 41.0 9 35.79049981898333 39.0 35.0 40.0 28.0 41.0 10-11 36.008571824070046 39.0 35.0 40.5 29.0 41.0 12-13 36.22103900529056 39.0 35.0 41.0 30.0 41.0 14-15 36.169159778399084 39.0 35.0 41.0 29.0 41.0 16-17 36.00725998557951 39.0 35.0 41.0 28.5 41.0 18-19 35.85752192735603 39.0 35.0 41.0 28.0 41.0 20-21 35.671993678106716 39.0 35.0 41.0 27.0 41.0 22-23 35.44019543716287 39.0 35.0 41.0 27.0 41.0 24-25 35.218834252614094 39.0 35.0 41.0 26.0 41.0 26-27 34.26347433366088 38.0 33.5 40.0 24.5 40.5 28-29 34.28032865935096 38.0 33.5 39.5 24.0 40.5 30-31 34.63818189285638 38.0 34.0 40.0 25.0 41.0 32-33 34.45826561687136 38.0 34.0 40.0 24.0 41.0 34-35 34.16410150319898 38.0 33.5 40.0 23.0 41.0 36-37 33.85395985384805 37.0 33.0 40.0 22.5 41.0 38-39 33.547438994338286 37.0 33.0 40.0 22.0 41.0 40-41 33.147847270603194 36.0 32.5 39.5 21.5 41.0 42-43 32.771133924958704 36.0 32.0 39.0 20.0 41.0 44-45 32.38879004803787 35.0 32.0 39.0 18.5 40.5 46-47 31.990171098786426 35.0 31.5 38.0 18.0 40.0 48-49 31.529693427725668 35.0 31.0 37.0 14.0 39.5 50-51 31.099198050496046 35.0 31.0 37.0 9.5 39.0 52-53 30.66636132145215 35.0 30.5 36.0 7.0 39.0 54-55 30.221276456575772 34.0 30.0 36.0 4.0 37.5 56-57 29.79582596843921 34.0 30.0 35.0 2.0 37.0 58-59 29.382319842774088 34.0 29.0 35.0 2.0 37.0 60-61 29.04737236803276 34.0 29.0 35.0 2.0 36.0 62-63 28.726271451997114 34.0 29.0 35.0 2.0 36.0 64-65 28.361461397813805 34.0 29.0 35.0 2.0 35.5 66-67 27.993310140888774 34.0 27.5 35.0 2.0 35.0 68-69 27.645214162501254 34.0 27.0 35.0 2.0 35.0 70-71 27.231718076416417 33.0 26.5 35.0 2.0 35.0 72-73 26.74648249614389 33.0 25.5 35.0 2.0 35.0 74-75 26.163229428747883 33.0 24.5 35.0 2.0 35.0 76-77 24.43222324984256 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8450227026408896 1101 2 0.8210616161248723 1101 3 0.9157994900586957 1101 4 0.9126622943313834 1101 5 1.038138503625568 1101 6 0.7772206282829188 1101 7 0.8673270438357363 1101 8 0.9610126069036724 1101 9 1.0000680417101435 1101 10-11 0.9174762762937263 1101 12-13 0.9778351960619176 1101 14-15 1.0472349162060581 1101 16-17 1.026468712750237 1101 18-19 1.0948304094376269 1101 20-21 0.9688759497112969 1101 22-23 1.0334365795329248 1101 24-25 1.0010756260385918 1101 26-27 0.81680745772168 1101 28-29 0.8882779709769437 1101 30-31 0.977221129191058 1101 32-33 1.0984792972977644 1101 34-35 1.021078686076045 1101 36-37 0.9494431523277385 1101 38-39 1.076242097572603 1101 40-41 1.0570152753384647 1101 42-43 0.9152177178252572 1101 44-45 0.8083613865935106 1101 46-47 0.8122191385965252 1101 48-49 0.9072537999634172 1101 50-51 0.719786835902152 1101 52-53 0.812131420439318 1101 54-55 0.7930052705967796 1101 56-57 0.7818956099123895 1101 58-59 0.896752910376879 1101 60-61 0.8516104448160888 1101 62-63 0.6779246198764746 1101 64-65 0.7271016963793464 1101 66-67 0.7422456655449636 1101 68-69 0.7839713004063498 1101 70-71 0.7787930728000987 1101 72-73 0.8474779828228947 1101 74-75 0.9152384630108017 1101 76-77 1.071849586094558 1102 1 0.7943192649108468 1102 2 0.9697307239453039 1102 3 0.80660440733012 1102 4 0.8202017524542242 1102 5 0.8736752994374797 1102 6 0.7717025465790357 1102 7 0.6000286048893244 1102 8 0.7774589062598949 1102 9 0.795542639704351 1102 10-11 0.7857862459530693 1102 12-13 0.8577277979337481 1102 14-15 0.7925350633282306 1102 16-17 0.7681601542841889 1102 18-19 0.8163662721769569 1102 20-21 0.8006187418309381 1102 22-23 0.9059902391046606 1102 24-25 0.7899749584599078 1102 26-27 0.7042154010549027 1102 28-29 0.662146304536698 1102 30-31 0.7928661551033045 1102 32-33 0.7447824974418396 1102 34-35 0.7583039114720478 1102 36-37 0.8030187369988084 1102 38-39 0.8425446447764884 1102 40-41 0.9000328699700333 1102 42-43 0.8139398507070013 1102 44-45 0.8681300035503696 1102 46-47 0.7081218336597672 1102 48-49 0.7492963021565551 1102 50-51 0.8652112522842117 1102 52-53 0.7834657142265904 1102 54-55 0.8707670759131858 1102 56-57 0.8847831325415072 1102 58-59 0.8849554397828818 1102 60-61 0.8268461614863867 1102 62-63 0.8522449038843902 1102 64-65 0.8781607758811312 1102 66-67 0.8438928124596607 1102 68-69 0.823468494343274 1102 70-71 0.8710046022492826 1102 72-73 0.9002056930775311 1102 74-75 0.7891158008262558 1102 76-77 1.1320103357541313 1103 1 0.47510626410175405 1103 2 0.4011325073014831 1103 3 0.40634889355669657 1103 4 0.4314330395812789 1103 5 0.4325138960610957 1103 6 0.4647885828246814 1103 7 0.4532496714654428 1103 8 0.6200502644864301 1103 9 0.4354577060037812 1103 10-11 0.5431732979655308 1103 12-13 0.5135137490780082 1103 14-15 0.6021552931438947 1103 16-17 0.6009064766884933 1103 18-19 0.6683751679969347 1103 20-21 0.628383593700029 1103 22-23 0.5388000731359952 1103 24-25 0.47838083758239947 1103 26-27 0.31985519161325726 1103 28-29 0.45156994176164034 1103 30-31 0.7280331508566249 1103 32-33 0.6039243987661465 1103 34-35 0.6693675111350004 1103 36-37 0.5707828133133717 1103 38-39 0.581876473114832 1103 40-41 0.6642216399405569 1103 42-43 0.6702096849898709 1103 44-45 0.6398433292539281 1103 46-47 0.4599540288144439 1103 48-49 0.5133047032985054 1103 50-51 0.5276418191936081 1103 52-53 0.5040562675533025 1103 54-55 0.5602268707475737 1103 56-57 0.6192372469630492 1103 58-59 0.5389913626298792 1103 60-61 0.5684127396979193 1103 62-63 0.41318776857318085 1103 64-65 0.4096002889167387 1103 66-67 0.46555646075197643 1103 68-69 0.47476440402680353 1103 70-71 0.5732877718777267 1103 72-73 0.5322030813452763 1103 74-75 0.4810916412548636 1103 76-77 0.5886519121839839 1104 1 0.5725970239603484 1104 2 0.45917096641331767 1104 3 0.48488143580279086 1104 4 0.40042274150913926 1104 5 0.5300134187448506 1104 6 0.34336211447415366 1104 7 0.6084213671690222 1104 8 0.6506226274842959 1104 9 0.6626622987963273 1104 10-11 0.6086576842605567 1104 12-13 0.6100661134531862 1104 14-15 0.6237572035634926 1104 16-17 0.7409164745565491 1104 18-19 0.6432370967999077 1104 20-21 0.6330219116994513 1104 22-23 0.6189273980166021 1104 24-25 0.6670162162209792 1104 26-27 0.5478707065560329 1104 28-29 0.6328453204497109 1104 30-31 0.7753022009292607 1104 32-33 0.813311178322742 1104 34-35 0.8287297960179458 1104 36-37 0.6315634696964025 1104 38-39 0.633047093774401 1104 40-41 0.7279012450732196 1104 42-43 0.7125352645631295 1104 44-45 0.6729642826328117 1104 46-47 0.5054153596009883 1104 48-49 0.5891287078312608 1104 50-51 0.5825930319067538 1104 52-53 0.6450389905610159 1104 54-55 0.6530221996050649 1104 56-57 0.595295559131582 1104 58-59 0.6390611250274212 1104 60-61 0.45349024637106794 1104 62-63 0.5166737441354172 1104 64-65 0.5019016006188828 1104 66-67 0.48932242816580995 1104 68-69 0.4050559751786764 1104 70-71 0.46933257280077356 1104 72-73 0.27939119384098277 1104 74-75 0.29639422470067345 1104 76-77 0.2685964754592085 1105 1 0.22440228098088966 1105 2 0.49678863109060245 1105 3 0.3611738317575899 1105 4 0.5648763111319681 1105 5 0.4897615894980447 1105 6 0.27399475648098814 1105 7 0.21625422698917873 1105 8 0.4761472273608902 1105 9 0.3074356393627866 1105 10-11 0.33186932866923513 1105 12-13 0.4641653963409098 1105 14-15 0.14067563665145144 1105 16-17 0.2935688824263494 1105 18-19 0.29458954916655244 1105 20-21 0.2991986588565254 1105 22-23 0.2634225308147151 1105 24-25 0.30806115271668233 1105 26-27 0.27746458672265106 1105 28-29 0.3855584607885234 1105 30-31 0.3179784121573803 1105 32-33 0.34719664891179747 1105 34-35 0.39535103027707663 1105 36-37 0.4526790500689444 1105 38-39 0.4451233288489007 1105 40-41 0.4608816082757201 1105 42-43 0.3068529733275298 1105 44-45 0.13457847360543695 1105 46-47 0.013851733607282313 1105 48-49 -0.03666740070172736 1105 50-51 0.20281038838391297 1105 52-53 0.0960206972734845 1105 54-55 -0.011537234424455534 1105 56-57 0.12921068404646263 1105 58-59 0.1274689981024011 1105 60-61 -0.1905623250635955 1105 62-63 -0.10698745780408814 1105 64-65 0.1330521843635175 1105 66-67 0.13731702749335994 1105 68-69 -0.14693292355287468 1105 70-71 -0.3750297454934959 1105 72-73 -0.33934221715015056 1105 74-75 -0.4203645052460381 1105 76-77 -0.16514408329999242 1106 1 0.1414077983808184 1106 2 0.07370853437189595 1106 3 -0.00598101259343764 1106 4 0.10725766034971684 1106 5 -0.10463705906738596 1106 6 0.1479888778227334 1106 7 0.14326167383699584 1106 8 0.17107914248160228 1106 9 0.08343248656283464 1106 10-11 -0.07459093496724023 1106 12-13 0.036241974896121576 1106 14-15 0.04086221836767123 1106 16-17 0.2174961401882669 1106 18-19 0.1247971598121822 1106 20-21 -0.00778548067129492 1106 22-23 0.17139771893588573 1106 24-25 0.20727618557886984 1106 26-27 0.08414596713998179 1106 28-29 0.06802439782283898 1106 30-31 0.02027137624091324 1106 32-33 0.04163209040642357 1106 34-35 0.007411413785639809 1106 36-37 0.10637671379491564 1106 38-39 -0.06578559505285142 1106 40-41 0.038766851283526194 1106 42-43 0.08363571133724435 1106 44-45 -0.16026548742792812 1106 46-47 0.10913772294195923 1106 48-49 0.058743079073963145 1106 50-51 -0.00721386313398753 1106 52-53 -0.06170371393701757 1106 54-55 0.10401745105501092 1106 56-57 0.0609447650750603 1106 58-59 -0.09136487643422342 1106 60-61 -0.08629059927508465 1106 62-63 -0.16773930617411636 1106 64-65 -0.036504001569579 1106 66-67 -0.03601866975574097 1106 68-69 -0.26170726025384994 1106 70-71 -0.4990695372773324 1106 72-73 -0.24666298375674245 1106 74-75 -0.3523960599294682 1106 76-77 -0.3200096787809805 1107 1 0.241448700144403 1107 2 0.2514566001309291 1107 3 0.17497045179281656 1107 4 0.1688007223451038 1107 5 0.2192437277588084 1107 6 -8.323608476530353E-4 1107 7 0.12162223491295521 1107 8 0.03193890442646108 1107 9 0.01978109332502953 1107 10-11 0.1550673118938093 1107 12-13 0.20145004113635423 1107 14-15 0.11000407187061967 1107 16-17 0.2046282391361629 1107 18-19 0.26239584820604733 1107 20-21 0.13623512831815532 1107 22-23 0.05255300471548452 1107 24-25 -0.1622715921492599 1107 26-27 -0.1608384111360195 1107 28-29 0.12574537905270944 1107 30-31 0.2969862428289858 1107 32-33 0.40051894375469743 1107 34-35 0.47547194839575013 1107 36-37 0.3935497145063991 1107 38-39 0.371491378629095 1107 40-41 0.38655007881074965 1107 42-43 0.3694778580267837 1107 44-45 0.44795095508103344 1107 46-47 0.3609224490548044 1107 48-49 0.5737000634797376 1107 50-51 0.43610454162849166 1107 52-53 0.4791636784208819 1107 54-55 0.3573746609360491 1107 56-57 0.4132522775947791 1107 58-59 0.38118385337697447 1107 60-61 0.3066443944177948 1107 62-63 0.378155565982091 1107 64-65 0.2858187374049912 1107 66-67 0.4334823630997171 1107 68-69 0.3029955489067717 1107 70-71 0.19808452488647177 1107 72-73 0.0980154905731041 1107 74-75 0.13305666072523437 1107 76-77 0.27661162931929084 1108 1 0.12685142842697417 1108 2 0.2083345924084199 1108 3 0.1439121985948404 1108 4 -0.0018819248407311306 1108 5 0.0706216155960746 1108 6 0.07930019939485788 1108 7 0.14057783088875198 1108 8 0.21730540748723115 1108 9 0.17925967029979972 1108 10-11 5.694930103956608E-4 1108 12-13 0.11321881618929552 1108 14-15 0.11661197960424374 1108 16-17 0.17513351054498827 1108 18-19 0.10021502641501456 1108 20-21 0.06954596898866328 1108 22-23 0.10483174655558969 1108 24-25 0.11945348780418641 1108 26-27 0.039301274737290726 1108 28-29 0.10691838587953129 1108 30-31 0.2108273095428146 1108 32-33 0.2275277433209979 1108 34-35 0.2614973157533669 1108 36-37 0.18870312874055628 1108 38-39 0.1316993485324005 1108 40-41 0.1761209559341097 1108 42-43 0.29642736129903824 1108 44-45 0.19495756975242529 1108 46-47 0.2109640245636868 1108 48-49 0.3002141457431762 1108 50-51 0.18446654680973396 1108 52-53 0.12351600961139653 1108 54-55 0.07849069070398329 1108 56-57 0.05450214197176706 1108 58-59 -0.03351358578998287 1108 60-61 -0.015914104464243906 1108 62-63 0.09660517500065424 1108 64-65 -0.09487154012051136 1108 66-67 -0.11486949192924101 1108 68-69 -0.1942191298926872 1108 70-71 -0.1548177516303575 1108 72-73 -0.05546269513568447 1108 74-75 -0.0968601014955972 1108 76-77 -0.013131138020842315 1109 1 0.03192756984114453 1109 2 0.03586155076313702 1109 3 -0.1890925956251337 1109 4 -0.027405595918871484 1109 5 0.1166559316714455 1109 6 0.10539148424675204 1109 7 -0.04923660814603181 1109 8 0.08332159499114766 1109 9 -0.04685348573553938 1109 10-11 -0.1350611630355445 1109 12-13 -0.07531823260559634 1109 14-15 0.04420224183689925 1109 16-17 0.19613517303774586 1109 18-19 0.08647285278704686 1109 20-21 0.17759239623186573 1109 22-23 0.18122849736503355 1109 24-25 0.16213637497008193 1109 26-27 0.06689658054026637 1109 28-29 0.2966556158658662 1109 30-31 0.33279813885190634 1109 32-33 0.28080014843941825 1109 34-35 0.3798050517889209 1109 36-37 0.35526614296671966 1109 38-39 0.34615766200962383 1109 40-41 0.402528477358679 1109 42-43 0.529202979454567 1109 44-45 0.40579393983359324 1109 46-47 0.11505737680805339 1109 48-49 0.22015604569853764 1109 50-51 0.34618532958409887 1109 52-53 0.4051833385412671 1109 54-55 0.531181718286657 1109 56-57 0.46779996291370907 1109 58-59 0.42244996475154295 1109 60-61 0.3253385769946959 1109 62-63 0.17880245590347954 1109 64-65 0.4000578805335273 1109 66-67 0.39297401409688604 1109 68-69 0.3328402376435946 1109 70-71 0.33591028067488793 1109 72-73 0.24554776296661984 1109 74-75 0.2514647025475547 1109 76-77 0.5131576002522671 1110 1 -0.4565641680106296 1110 2 -0.5867765441019657 1110 3 -0.5647172774210887 1110 4 -0.44871695636129516 1110 5 -0.38230277261532564 1110 6 -0.6092786446014102 1110 7 -0.6535862241152941 1110 8 -0.6241572817246492 1110 9 -0.49078809531567913 1110 10-11 -0.5218564805291876 1110 12-13 -0.42564993898078995 1110 14-15 -0.5034044083203426 1110 16-17 -0.5579425293172662 1110 18-19 -0.5278623819530495 1110 20-21 -0.6846758669728672 1110 22-23 -0.5667678759057324 1110 24-25 -0.6220663138370597 1110 26-27 -0.38550683343056136 1110 28-29 -0.39294094466144003 1110 30-31 -0.37447494693727634 1110 32-33 -0.1197305635452679 1110 34-35 -0.00899630600312662 1110 36-37 -0.08955265263493573 1110 38-39 -0.13630939732501446 1110 40-41 -0.15432018456767338 1110 42-43 -0.12423376089729743 1110 44-45 -0.21853884490609943 1110 46-47 -0.3345462969174733 1110 48-49 -0.29172540889129905 1110 50-51 -0.297914996566508 1110 52-53 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2314 5 -0.5085287727752714 2314 6 -0.4795987304738034 2314 7 -0.45460653438503584 2314 8 -0.49617895919264754 2314 9 -0.50248395686625 2314 10-11 -0.4727279063310874 2314 12-13 -0.5506157666990745 2314 14-15 -0.5421090359341889 2314 16-17 -1.0473001947989857 2314 18-19 -0.7536453435651254 2314 20-21 -0.6019529274674369 2314 22-23 -0.44378622786005195 2314 24-25 -0.4772591882581949 2314 26-27 -0.47518909913303986 2314 28-29 -0.5836376647210315 2314 30-31 -0.7098809382036038 2314 32-33 -0.7219281348227753 2314 34-35 -0.6890242133025737 2314 36-37 -0.804924216046949 2314 38-39 -0.6607695750469773 2314 40-41 -0.4921512374208561 2314 42-43 -0.4378566014431797 2314 44-45 -0.4580721493922155 2314 46-47 -0.41992657742719786 2314 48-49 -0.39395778562587225 2314 50-51 -0.4065357672046126 2314 52-53 -0.42826436104240173 2314 54-55 -0.479236614990981 2314 56-57 -0.3181821059290826 2314 58-59 -0.44798923144240277 2314 60-61 -0.3595840808490465 2314 62-63 -0.26592172643889 2314 64-65 -0.26749070264417085 2314 66-67 -0.36050220592273874 2314 68-69 -0.19294482922684253 2314 70-71 -0.10772276550816784 2314 72-73 -0.09084445824953846 2314 74-75 -0.08539413619079639 2314 76-77 0.06450106819072943 2315 1 -0.9048943804384706 2315 2 -0.8343455856079487 2315 3 -0.7774744982634658 2315 4 -0.8573823162777003 2315 5 -0.6857831454992791 2315 6 -0.7606378013254442 2315 7 -0.7688152274976829 2315 8 -0.7598365459334957 2315 9 -0.6364126873528804 2315 10-11 -0.6765499447787136 2315 12-13 -0.7078938747932639 2315 14-15 -0.9651315875517099 2315 16-17 -0.8060039821201954 2315 18-19 -0.691278277278478 2315 20-21 -0.7321633714511364 2315 22-23 -0.7728296676132942 2315 24-25 -0.9108429696543325 2315 26-27 -0.8426552301863452 2315 28-29 -0.839494287711311 2315 30-31 -0.9253949714729615 2315 32-33 -0.9248304118949662 2315 34-35 -0.9162123567550466 2315 36-37 -1.0816231409908 2315 38-39 -0.9105156131787098 2315 40-41 -0.8291528504048458 2315 42-43 -0.9046739339163636 2315 44-45 -0.9477561846379992 2315 46-47 -0.7778116621014917 2315 48-49 -0.7997744732701655 2315 50-51 -0.7435222308476668 2315 52-53 -0.8095432193095142 2315 54-55 -0.8263205351859391 2315 56-57 -0.9038466493034001 2315 58-59 -0.7523067725697103 2315 60-61 -0.9498942818401233 2315 62-63 -0.9327130595570488 2315 64-65 -0.8891576196957764 2315 66-67 -0.7704177721931771 2315 68-69 -0.8351515874320015 2315 70-71 -0.8786958006631949 2315 72-73 -2.440109497792829 2315 74-75 -2.8838171459075603 2315 76-77 -2.5641828692856947 2316 1 -3.1410740440675013 2316 2 -2.960257098563261 2316 3 -2.488772944935313 2316 4 -2.553042359258761 2316 5 -3.28478785715226 2316 6 -2.7566734079743256 2316 7 -2.92348855057665 2316 8 -2.950227590321809 2316 9 -2.4848607971876504 2316 10-11 -2.5462039640636576 2316 12-13 -2.6024967957999436 2316 14-15 -2.7136251676641905 2316 16-17 -3.720806218642984 2316 18-19 -3.5666649233967505 2316 20-21 -2.915486440501496 2316 22-23 -2.9380590867342207 2316 24-25 -2.9715730626477423 2316 26-27 -2.730247199649355 2316 28-29 -2.8571714160128643 2316 30-31 -3.125675925228144 2316 32-33 -3.1939832168210245 2316 34-35 -3.223949444336654 2316 36-37 -3.0854370411109002 2316 38-39 -3.0398941728270827 2316 40-41 -3.0213545242267656 2316 42-43 -3.237432566575034 2316 44-45 -3.112999983672264 2316 46-47 -2.804490368226645 2316 48-49 -2.987568703652247 2316 50-51 -3.4069945733157176 2316 52-53 -4.459959840109214 2316 54-55 -3.693406004432262 2316 56-57 -3.5818134485627837 2316 58-59 -3.5357675912727196 2316 60-61 -3.3474267382962566 2316 62-63 -3.2928622040513744 2316 64-65 -3.3667405744407013 2316 66-67 -3.4488072913189605 2316 68-69 -3.5689032770718185 2316 70-71 -3.2242195815688497 2316 72-73 -3.168058644468932 2316 74-75 -3.3369443307866327 2316 76-77 -3.3103057471327944 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78576.0 3 6687.0 4 8030.0 5 9263.0 6 10908.0 7 11934.0 8 13561.0 9 13848.0 10 15917.0 11 17298.0 12 15158.0 13 13978.0 14 14012.0 15 14386.0 16 15118.0 17 16060.0 18 16951.0 19 18160.0 20 19568.0 21 21355.0 22 23754.0 23 27379.0 24 32128.0 25 37523.0 26 44367.0 27 52270.0 28 62166.0 29 74321.0 30 89064.0 31 107189.0 32 132689.0 33 168588.0 34 226250.0 35 305164.0 36 431226.0 37 553084.0 38 453816.0 39 114424.0 40 820.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.60924244720023 25.782054862958226 27.3258400164057 23.282862673435844 2 23.152379758482446 26.078120778250273 27.514580630399276 23.254918832868 3 23.29754676828574 26.061745516993344 26.81631219873167 23.824395515989245 4 23.33490340413257 26.26786259811421 27.133866183717757 23.26336781403546 5 23.416767886743862 26.400397009889954 26.97859895185494 23.204236151511243 6 23.503062834895356 26.086140310789585 26.88321065056603 23.52758620374903 7 23.465634876707654 25.988868863946795 26.903622959314543 23.641873300031005 8 23.30480663462548 26.074467615730917 26.64250645464103 23.978219295002578 9 23.057036913970858 25.962101973041605 27.08323824442444 23.89762286856309 10-11 23.775704671396785 26.03957497735308 26.82397843909858 23.360741912151557 12-13 23.880415354964427 25.740664219846266 26.82285279947128 23.556067625718025 14-15 23.51509831647629 25.97856669630626 26.83913916890992 23.667195818307533 16-17 23.785218608699807 25.945375930110202 26.91162718951361 23.35777827167638 18-19 23.677699705942494 25.917530014313396 26.688776764588468 23.715993515155645 20-21 23.532754594480114 25.999855152993128 26.884912967032697 23.582477285494058 22-23 23.88379456141872 26.1730316598886 26.442267754200348 23.50090602449233 24-25 23.648717421676846 26.259015080019175 26.349723106218914 23.742544392085062 26-27 23.720520062880794 26.299442094225036 26.487408930265733 23.492628912628437 28-29 23.99111144511734 26.08368245520543 26.40053537006817 23.52467072960906 30-31 23.645519537360084 26.142368203028994 26.51663384248592 23.695478417125003 32-33 23.512514420172526 26.385565025070523 26.41841023407422 23.68351032068273 34-35 23.54420985997195 26.035970700952642 26.595243126503554 23.824576312571853 36-37 23.357694215777506 26.211932222800833 26.72432770247502 23.70604585894664 38-39 23.498850904868675 26.115784608484592 26.832869871556326 23.552494615090406 40-41 23.335636112424098 26.151639296253265 26.653554872751045 23.85916971857159 42-43 23.286622110722487 26.27902589528998 26.5282876490375 23.906064344950032 44-45 23.567510776083385 25.939580299830173 26.73845498964647 23.75445393443997 46-47 23.48720888727407 25.872482333082896 26.57226661457045 24.06804216507259 48-49 23.508795526321915 26.086053548358922 26.467705004907575 23.937445920411584 50-51 23.358361758125106 26.339099853189296 26.425074196619214 23.877464192066377 52-53 23.541371580012893 26.001717107589783 26.585159345271318 23.871751967126002 54-55 23.452731905155698 26.18628794270451 26.54576936800134 23.81521078413845 56-57 23.606920332676467 26.18089678533653 26.348774737426346 23.86340814456066 58-59 23.61356372737628 26.145956899170887 26.51054410730828 23.72993526614455 60-61 23.42965114651457 26.225645922415065 26.41538772385303 23.92931520721734 62-63 23.52865636328364 26.23727704638044 26.341107150872418 23.892959439463503 64-65 23.480995911811746 26.107347616506672 26.28312951731565 24.128526954365938 66-67 23.27109471607514 26.20232954701934 26.45503112438051 24.07154461252501 68-69 23.415651725058336 25.95444381443346 26.634383929911127 23.995520530597076 70-71 23.242585675865016 25.80656453223968 26.785958614051825 24.16489117784348 72-73 23.370058840992307 25.515296374390083 26.83001575690221 24.2846290277154 74-75 23.170831406845714 25.71070456238448 26.637717463470068 24.48074656729974 76-77 23.180002137564777 25.536286684585264 26.861439823440804 24.422271354409155 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4154.0 1 2544.0 2 926.5 3 920.0 4 921.0 5 972.5 6 1087.5 7 1210.5 8 1315.0 9 1360.0 10 1415.5 11 1562.5 12 1754.0 13 1854.0 14 2025.5 15 2391.0 16 2883.0 17 3181.0 18 3417.0 19 4084.0 20 5031.5 21 6426.0 22 7304.0 23 9069.0 24 12401.0 25 16405.0 26 18842.0 27 20553.5 28 27776.5 29 35011.5 30 36735.0 31 41970.0 32 51631.5 33 61260.0 34 74854.0 35 83246.0 36 87855.5 37 97119.5 38 105202.5 39 108631.0 40 113962.5 41 123447.0 42 132893.0 43 138186.0 44 140589.0 45 147320.0 46 154590.5 47 160868.5 48 164204.0 49 165270.0 50 165268.5 51 161504.0 52 158807.0 53 157668.5 54 152896.5 55 141293.0 56 128826.0 57 124329.0 58 118334.5 59 102230.0 60 83615.5 61 75111.0 62 66926.5 63 51173.5 64 37844.5 65 32084.0 66 27028.5 67 19020.5 68 14194.5 69 10361.5 70 8402.0 71 7119.5 72 5053.5 73 3857.5 74 3445.0 75 3086.5 76 2173.0 77 1407.5 78 1197.0 79 1037.0 80 776.5 81 539.5 82 330.5 83 258.0 84 215.5 85 159.5 86 147.5 87 149.0 88 119.0 89 81.5 90 71.0 91 68.0 92 60.5 93 58.5 94 54.0 95 49.5 96 55.0 97 48.0 98 50.5 99 122.5 100 185.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009978734343578776 2 0.013325261105144828 3 0.014237950221935572 4 0.015637406867681374 5 0.012838493576189765 6 0.010009157314138466 7 0.01621544330831551 8 0.01810166748301638 9 0.0180103985713373 10-11 0.02041381324555292 12-13 0.01667178786671088 14-15 0.008594489183112817 16-17 0.006556150155613494 18-19 0.016976017572307798 20-21 0.02324314950760422 22-23 0.019927045716597862 24-25 0.018907876202848197 26-27 0.02348653327208175 28-29 0.00886829591815004 30-31 0.008731392550631429 32-33 0.010997903857328437 34-35 0.012123553768037018 36-37 0.011210864651246277 38-39 0.014937678544808472 40-41 0.011606363268522265 42-43 0.014755140721450325 44-45 0.01306666585538745 46-47 0.02321272653704453 48-49 0.02268032455224993 50-51 0.0342562648502125 52-53 0.037481099729539794 54-55 0.026391926960532282 56-57 0.020505082157231996 58-59 0.016230654793595357 60-61 0.020748465921709525 62-63 0.025935582402136913 64-65 0.02480993249142833 66-67 0.027395884989002098 68-69 0.04055381975606862 70-71 0.053072872141381636 72-73 0.08259836506956213 74-75 0.08310034408379703 76-77 0.08758773224135151 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3286990.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.63876721351328 #Duplication Level Percentage of deduplicated Percentage of total 1 71.28863698188238 34.67391419130432 2 13.870571794428646 13.492950252550761 3 5.690812892455361 8.303823705953894 4 2.873541408154555 5.590620465184823 5 1.6598612186699593 4.036680171081331 6 1.0511589701309278 3.067624587155674 7 0.7429111376018946 2.5293997317501424 8 0.5068404334630557 1.972167508608459 9 0.3622976214815857 1.5859538705927156 >10 1.801166376630384 15.336393919895864 >50 0.1032505558379493 3.421406262599254 >100 0.044868427773420545 3.675167665304683 >500 0.0022609005185527304 0.7548652040207903 >1k 0.0018212809713001514 1.5590324639973425 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4415 0.1343174150210375 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2169188223876556E-4 3.042297055969139E-5 2 0.0 0.0 0.0 3.3465267615660527E-4 6.084594111938278E-5 3 0.0 0.0 0.0 3.9549861727598807E-4 6.084594111938278E-5 4 0.0 0.0 0.0 8.822661462310503E-4 6.084594111938278E-5 5 0.0 0.0 0.0 0.0023121457625365455 6.084594111938278E-5 6 0.0 0.0 0.0 0.00438090776059556 6.084594111938278E-5 7 0.0 0.0 0.0 0.010100426225817541 9.126891167907417E-5 8 0.0 0.0 0.0 0.016032905484957363 1.2169188223876556E-4 9 0.0 0.0 0.0 0.022543421184731318 1.2169188223876556E-4 10 0.0 0.0 0.0 0.030027471942415403 1.2169188223876556E-4 11 0.0 0.0 0.0 0.0385154807285693 1.2169188223876556E-4 12 0.0 0.0 0.0 0.05126270539307999 1.2169188223876556E-4 13 0.0 0.0 0.0 0.06787364731867149 1.2169188223876556E-4 14 0.0 0.0 0.0 0.08764857818247089 1.2169188223876556E-4 15 0.0 0.0 3.042297055969139E-5 0.10943142510320993 1.2169188223876556E-4 16 0.0 0.0 3.042297055969139E-5 0.1310317342005908 1.2169188223876556E-4 17 0.0 0.0 3.042297055969139E-5 0.15311881082692677 1.2169188223876556E-4 18 0.0 0.0 3.042297055969139E-5 0.1742323523953526 1.2169188223876556E-4 19 0.0 0.0 3.042297055969139E-5 0.2013392191640376 1.8253782335814835E-4 20 0.0 0.0 3.042297055969139E-5 0.22993681149014752 1.8253782335814835E-4 21 0.0 0.0 3.042297055969139E-5 0.26197219948950257 2.1296079391783972E-4 22 0.0 0.0 3.042297055969139E-5 0.2947985847234096 2.1296079391783972E-4 23 0.0 0.0 3.042297055969139E-5 0.3278683537217941 2.738067350372225E-4 24 0.0 0.0 3.042297055969139E-5 0.36245927124816324 3.042297055969139E-4 25 0.0 0.0 3.042297055969139E-5 0.3965329982750176 3.042297055969139E-4 26 0.0 0.0 3.042297055969139E-5 0.43267548729993094 3.650756467162967E-4 27 0.0 0.0 3.042297055969139E-5 0.47033912485282886 3.650756467162967E-4 28 0.0 0.0 3.042297055969139E-5 0.5070900732889361 4.2592158783567944E-4 29 0.0 0.0 3.042297055969139E-5 0.5459097837231023 4.563445583953708E-4 30 0.0 0.0 3.042297055969139E-5 0.5879543290365958 4.8676752895506224E-4 31 0.0 0.0 3.042297055969139E-5 0.6333454011116554 4.8676752895506224E-4 32 0.0 0.0 3.042297055969139E-5 0.6828131512417135 4.8676752895506224E-4 33 0.0 0.0 3.042297055969139E-5 0.7355665821922184 4.8676752895506224E-4 34 0.0 0.0 3.042297055969139E-5 0.7915144250514908 4.8676752895506224E-4 35 0.0 0.0 3.042297055969139E-5 0.8484662259392332 4.8676752895506224E-4 36 0.0 0.0 3.042297055969139E-5 0.906543676737684 5.171904995147536E-4 37 0.0 0.0 3.042297055969139E-5 0.9679980772682606 5.171904995147536E-4 38 0.0 0.0 3.042297055969139E-5 1.031947161384732 5.780364406341364E-4 39 0.0 0.0 3.042297055969139E-5 1.096109206295121 5.780364406341364E-4 40 0.0 0.0 3.042297055969139E-5 1.1688505289033433 5.780364406341364E-4 41 0.0 0.0 3.042297055969139E-5 1.2419265041877219 5.780364406341364E-4 42 0.0 0.0 3.042297055969139E-5 1.3282060486950067 5.780364406341364E-4 43 0.0 0.0 3.042297055969139E-5 1.4194141144329615 6.388823817535192E-4 44 0.0 0.0 3.042297055969139E-5 1.5122954435516993 6.388823817535192E-4 45 0.0 0.0 3.042297055969139E-5 1.6080669548736077 6.388823817535192E-4 46 0.0 0.0 3.042297055969139E-5 1.7107749034831259 6.388823817535192E-4 47 3.042297055969139E-5 0.0 3.042297055969139E-5 1.8127527007992115 6.693053523132105E-4 48 3.042297055969139E-5 0.0 3.042297055969139E-5 1.9227317393724959 6.99728322872902E-4 49 3.042297055969139E-5 0.0 3.042297055969139E-5 2.032528240122422 7.301512934325934E-4 50 3.042297055969139E-5 0.0 3.042297055969139E-5 2.1512690942168975 7.301512934325934E-4 51 3.042297055969139E-5 0.0 3.042297055969139E-5 2.276033696482192 7.909972345519761E-4 52 3.042297055969139E-5 0.0 3.042297055969139E-5 2.4077651590056557 7.909972345519761E-4 53 3.042297055969139E-5 0.0 3.042297055969139E-5 2.555103605426241 7.909972345519761E-4 54 3.042297055969139E-5 0.0 3.042297055969139E-5 2.7076443798125336 8.214202051116676E-4 55 3.042297055969139E-5 0.0 3.042297055969139E-5 2.8617671486679304 8.518431756713589E-4 56 6.084594111938278E-5 0.0 3.042297055969139E-5 3.023769466898287 8.518431756713589E-4 57 6.084594111938278E-5 0.0 3.042297055969139E-5 3.1879622390089413 8.518431756713589E-4 58 6.084594111938278E-5 0.0 3.042297055969139E-5 3.3689180678979858 9.431120873504331E-4 59 6.084594111938278E-5 3.042297055969139E-5 3.042297055969139E-5 3.5496305130225525 9.431120873504331E-4 60 6.084594111938278E-5 6.084594111938278E-5 3.042297055969139E-5 3.737583625140326 9.735350579101245E-4 61 6.084594111938278E-5 6.084594111938278E-5 3.042297055969139E-5 3.938192693010931 0.0010039580284698158 62 6.084594111938278E-5 6.084594111938278E-5 3.042297055969139E-5 4.144551702317318 0.0010039580284698158 63 6.084594111938278E-5 6.084594111938278E-5 3.042297055969139E-5 4.359064067733701 0.0010648039695891987 64 6.084594111938278E-5 6.084594111938278E-5 3.042297055969139E-5 4.582916285111911 0.0010648039695891987 65 6.084594111938278E-5 6.084594111938278E-5 3.042297055969139E-5 4.822710139063399 0.0010648039695891987 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACGTA 95 0.006653569 18.68077 32 TAAGGCG 1440 0.0 18.242195 17 CGGTCGA 120 0.0012158629 17.759163 67 CGCGGAA 245 1.1174052E-8 17.3872 59 GACGCGA 1025 0.0 16.633986 69 CTCGACG 150 2.8419925E-4 16.563112 30 CCGGGGT 1185 0.0 16.478273 48 GTAACGT 260 2.3295797E-8 16.382347 2 GGTAACG 285 4.110916E-9 16.190989 1 CGGGGTG 1165 0.0 16.15191 49 GGACGCG 1160 0.0 15.6165285 68 ATTACCG 310 1.2503733E-8 14.8845825 3 GGCGGAA 1755 0.0 14.766135 20 CGCGACC 1280 0.0 14.708374 71 AGGCGGA 1915 0.0 14.459289 19 TCGCGGA 300 1.3470344E-7 14.199546 58 TACCGCG 230 3.272904E-5 13.888958 5 TGGACGC 1310 0.0 13.827745 67 GCGGAAG 1930 0.0 13.795106 21 ACGCGAC 1290 0.0 13.76826 70 >>END_MODULE