FastQCFastQC Report
Thu 26 May 2016
SRR936009_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936009_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3286990
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51650.15713464294080604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38370.11673293803753586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65450.037.1867521
CGTATCA12700.037.0263100-101
CGTACAT18750.031.269571100-101
GTATCAA128300.031.1222131
CCGTACA9000.028.438906100-101
GCGTACA6450.027.685255100-101
CGGTATC12600.027.635815100-101
CCTGTCG11550.027.05316296-97
TACGCAG8300.026.175907100-101
TTATACG8500.025.20717896-97
TCGAGTA4850.025.15933100-101
AACGTAT3450.025.01665598-99
AGTATCA27500.024.675112100-101
TCGTACA10750.024.363024100-101
CCCGTAC4600.023.93835398-99
ATCAACG164500.023.8707873
GGGTATC13300.023.719809100-101
TCGGTAT3900.023.656694100-101
ACGTATC4800.023.561302100-101
TCAACGC167100.023.499014
Produced by FastQC (version 0.11.5)