FastQCFastQC Report
Thu 26 May 2016
SRR936008_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936008_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298496
Sequences flagged as poor quality0
Sequence length77
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45550.13809324007062612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA2052.7584974E-819.0495357
AATTCGC1953.4089499E-718.20473955
ATTCGCG2852.1827873E-1017.43743156
CGGTTAC2451.1192242E-817.3847484
TCGAACT6600.017.21149324
CGCGTTG1350.00268415915.77600710
TAAGGCG14700.015.21257717
CGAACTC7600.014.94546325
CTCGAAC8250.014.19948223
CCTCGAA8300.014.11394222
ACGACGG1800.001132497913.80505224
CGATCGT2851.1725697E-613.6985531
TACGACG1600.00828880913.31201523
CGCTCCG4001.8353603E-913.31080225
TAGCGCG1600.00829545713.3103995
CCGGGGT12300.013.27835548
CAATTCG2951.7236143E-613.23740754
GCCTCGA8400.013.10071321
GACGCGA11250.012.94417669
GGCGGAA17600.012.90861920
Produced by FastQC (version 0.11.5)