##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936008_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3298496 Sequences flagged as poor quality 0 Sequence length 77 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.7113814902307 40.0 36.0 41.0 30.0 41.0 2 36.577078159258036 39.0 36.0 41.0 30.0 41.0 3 36.442855471099556 39.0 36.0 41.0 30.0 41.0 4 36.34551444052077 39.0 36.0 41.0 30.0 41.0 5 36.26001092619182 39.0 36.0 41.0 29.0 41.0 6 36.17785621083063 39.0 36.0 41.0 29.0 41.0 7 36.03635050641262 39.0 35.0 40.0 29.0 41.0 8 35.91095335571121 39.0 35.0 40.0 28.0 41.0 9 36.08684624750189 39.0 35.0 41.0 29.0 41.0 10-11 36.32828719513378 39.0 36.0 41.0 30.0 41.0 12-13 36.26358831418925 40.0 35.5 41.0 29.5 41.0 14-15 36.1109434421021 39.0 35.0 41.0 29.0 41.0 16-17 35.963223996633616 39.0 35.0 41.0 28.0 41.0 18-19 35.81036372334543 39.0 35.0 41.0 28.0 41.0 20-21 35.62830393003357 39.0 35.0 41.0 27.0 41.0 22-23 35.3902164804808 39.0 35.0 41.0 26.5 41.0 24-25 35.1684604134733 39.0 35.0 41.0 26.0 41.0 26-27 34.19328854726518 38.0 33.0 40.0 24.5 40.5 28-29 34.19297613215235 38.0 33.5 39.5 24.0 40.5 30-31 34.585677078280526 38.0 34.0 40.0 24.0 41.0 32-33 34.43705328125303 38.0 34.0 40.0 24.0 41.0 34-35 34.16075568986592 38.0 33.5 40.0 23.0 41.0 36-37 33.82983471861115 37.0 33.0 40.0 22.5 41.0 38-39 33.502300593967675 37.0 33.0 40.0 22.0 41.0 40-41 33.12374154766293 36.0 32.5 39.5 20.5 41.0 42-43 32.73814884116882 36.0 32.0 39.0 20.0 41.0 44-45 32.36524449324783 35.0 32.0 39.0 18.5 40.5 46-47 31.937443762248005 35.0 31.5 38.0 17.5 40.0 48-49 31.525613946477428 35.0 31.0 37.0 14.0 39.5 50-51 31.084217928413434 35.0 31.0 37.0 9.5 39.0 52-53 30.61981248423524 35.0 30.5 36.0 7.0 39.0 54-55 30.168640343962824 34.0 30.0 36.0 3.5 37.5 56-57 29.75354510055492 34.0 30.0 35.0 2.0 37.0 58-59 29.34520490550845 34.0 29.0 35.0 2.0 37.0 60-61 28.99786190433458 34.0 29.0 35.0 2.0 36.0 62-63 28.648854205068005 34.0 29.0 35.0 2.0 36.0 64-65 28.27970217335416 34.0 29.0 35.0 2.0 35.5 66-67 27.94090382404587 34.0 27.5 35.0 2.0 35.0 68-69 27.58089505035022 34.0 27.0 35.0 2.0 35.0 70-71 27.139747478850968 33.0 26.0 35.0 2.0 35.0 72-73 26.66402339126681 33.0 25.0 35.0 2.0 35.0 74-75 26.09257249364559 33.0 24.5 35.0 2.0 35.0 76-77 24.3078509114457 31.0 19.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8950172300610433 1101 2 0.7259640215549439 1101 3 0.8204145840343813 1101 4 0.7086455569107102 1101 5 0.8103805194049443 1101 6 0.7527626988314466 1101 7 0.7819730382900403 1101 8 0.7991685838848568 1101 9 0.8373473309147172 1101 10-11 0.7973900649397763 1101 12-13 0.916240881031051 1101 14-15 0.9140020421955057 1101 16-17 0.9294356556081809 1101 18-19 0.8496960776383773 1101 20-21 0.9061795890641662 1101 22-23 0.8784266078045277 1101 24-25 0.9174807648068324 1101 26-27 0.8055545045682422 1101 28-29 0.9335896076479813 1101 30-31 0.8680591291823916 1101 32-33 0.9706736499487576 1101 34-35 0.9985469455680516 1101 36-37 0.9914392479274383 1101 38-39 0.8371985224880873 1101 40-41 0.8227694365461815 1101 42-43 0.8491158088179915 1101 44-45 0.6869400523370359 1101 46-47 0.8347822180652713 1101 48-49 0.6611239387542653 1101 50-51 0.6138029181586298 1101 52-53 0.6997232130484257 1101 54-55 0.8222970265378002 1101 56-57 0.8099303132212619 1101 58-59 0.9058717550459612 1101 60-61 0.6933918449787093 1101 62-63 0.7343033616827768 1101 64-65 0.6813106856993265 1101 66-67 0.8011132303074362 1101 68-69 0.8434174446116423 1101 70-71 0.7054785451918129 1101 72-73 0.5735545915777323 1101 74-75 0.6355730463849092 1101 76-77 0.9214167342950716 1102 1 0.8317505666786147 1102 2 0.801606472005453 1102 3 0.8493280898345361 1102 4 0.6629201475434598 1102 5 0.7605150964610061 1102 6 0.9438036702076786 1102 7 0.7242407963243807 1102 8 0.8706110069481952 1102 9 0.8894458938396639 1102 10-11 0.9656318940855755 1102 12-13 1.0303709187468613 1102 14-15 0.9580671042633568 1102 16-17 1.0203304855279995 1102 18-19 0.957734765776145 1102 20-21 0.9288640788236222 1102 22-23 0.9158329027626948 1102 24-25 0.9588529289194483 1102 26-27 0.8394674217111913 1102 28-29 0.8844919439908452 1102 30-31 1.062482426731485 1102 32-33 1.1026334776433657 1102 34-35 0.9822530916502288 1102 36-37 1.0361233915898538 1102 38-39 1.0790538134308605 1102 40-41 1.0431084195970328 1102 42-43 1.060586211885358 1102 44-45 0.9498870665090635 1102 46-47 0.9223437765790479 1102 48-49 0.9236333217830719 1102 50-51 0.9033642829582682 1102 52-53 0.834007140786369 1102 54-55 0.9139769414705761 1102 56-57 0.9783207156874418 1102 58-59 0.9842109652099431 1102 60-61 0.8505274661707034 1102 62-63 0.9313331894859331 1102 64-65 0.9571230952611245 1102 66-67 0.8835048860707246 1102 68-69 0.6932870006806127 1102 70-71 0.7356556287251905 1102 72-73 0.9655147031637625 1102 74-75 0.8992910553709059 1102 76-77 0.8923403040459412 1103 1 0.41989141057205615 1103 2 0.36308806020153384 1103 3 0.3671976277054938 1103 4 0.38368115723879725 1103 5 0.26160837967654516 1103 6 0.3670215801221133 1103 7 0.33687436095374323 1103 8 0.3052463487128847 1103 9 0.4807969324966592 1103 10-11 0.4475657847726353 1103 12-13 0.3903841293999264 1103 14-15 0.37896576455259634 1103 16-17 0.37200772149274286 1103 18-19 0.3971377996535068 1103 20-21 0.34691381898610985 1103 22-23 0.21277950989205863 1103 24-25 0.3701678461829019 1103 26-27 0.4232961882572681 1103 28-29 0.3891353499396075 1103 30-31 0.5456615565300424 1103 32-33 0.42030744564583244 1103 34-35 0.630948107162979 1103 36-37 0.6487083950806962 1103 38-39 0.6129566711223404 1103 40-41 0.6899000914022366 1103 42-43 0.6258591098026614 1103 44-45 0.514394822987498 1103 46-47 0.5453599655474335 1103 48-49 0.5933288545731159 1103 50-51 0.5489598838047272 1103 52-53 0.6347667078112131 1103 54-55 0.6888464915829893 1103 56-57 0.6786553423994839 1103 58-59 0.6834500324030763 1103 60-61 0.5970375643077048 1103 62-63 0.5324152227043797 1103 64-65 0.4130971118146043 1103 66-67 0.5520557923581499 1103 68-69 0.497442862915495 1103 70-71 0.5160758687244531 1103 72-73 0.4569019805703789 1103 74-75 0.5312644980935985 1103 76-77 0.6357303996172945 1104 1 0.2549370320404378 1104 2 0.25700388165331844 1104 3 0.23717124898641373 1104 4 0.443114554837706 1104 5 0.32877826144344624 1104 6 0.44297153548476587 1104 7 0.33162608778950897 1104 8 0.2558829638637121 1104 9 0.29409259066643045 1104 10-11 0.5157847697901019 1104 12-13 0.5901020776710055 1104 14-15 0.7427801011417401 1104 16-17 0.6406509656370432 1104 18-19 0.4065544244003547 1104 20-21 0.4118298764492039 1104 22-23 0.4328753165018142 1104 24-25 0.5224943397175039 1104 26-27 0.3462735482054242 1104 28-29 0.37384852235398824 1104 30-31 0.5257137271399301 1104 32-33 0.6154160842012573 1104 34-35 0.6270888250410493 1104 36-37 0.5275715787245687 1104 38-39 0.4818783337801662 1104 40-41 0.5202484281428212 1104 42-43 0.5622080808297838 1104 44-45 0.509224826414929 1104 46-47 0.36296605469826915 1104 48-49 0.2794584788366201 1104 50-51 0.2329681139729587 1104 52-53 0.2763487791437349 1104 54-55 0.22918709617168886 1104 56-57 0.2736390633299486 1104 58-59 0.10173301222652142 1104 60-61 0.1492018234522554 1104 62-63 0.15298554299742406 1104 64-65 0.1356323911729298 1104 66-67 0.19376000471200783 1104 68-69 -0.11887723537233441 1104 70-71 0.06325966895550295 1104 72-73 0.21271510057390586 1104 74-75 0.17461970653431536 1104 76-77 0.08823221302500173 1105 1 -0.0372742003984996 1105 2 0.10142401805637746 1105 3 -0.021460808744322435 1105 4 0.11181062438766531 1105 5 0.0031231027712053105 1105 6 0.07877307142861412 1105 7 0.18783977535993301 1105 8 0.18102722890360923 1105 9 0.21943678403718536 1105 10-11 0.2982578224244463 1105 12-13 0.34091564406806896 1105 14-15 0.27939961522805135 1105 16-17 0.09683591411326375 1105 18-19 0.10304603455420391 1105 20-21 0.11123396259428375 1105 22-23 0.1673096747502143 1105 24-25 0.1215175580481187 1105 26-27 -0.03696188685638191 1105 28-29 0.05733393057848701 1105 30-31 0.2807027161184905 1105 32-33 0.24887799529599164 1105 34-35 0.21072721560543783 1105 36-37 0.2841982713095135 1105 38-39 0.23622240667262417 1105 40-41 0.3170312231466781 1105 42-43 0.36278005348430753 1105 44-45 0.21650576056968163 1105 46-47 0.20185456709247518 1105 48-49 -0.027130844237525764 1105 50-51 0.1057351626176164 1105 52-53 0.04760731012712327 1105 54-55 -0.0994318962281362 1105 56-57 0.11904836711211786 1105 58-59 0.09039149602257979 1105 60-61 0.1719238568479149 1105 62-63 0.19970158259771154 1105 64-65 0.1683275274022229 1105 66-67 0.1624246900512638 1105 68-69 0.004681851896432221 1105 70-71 -0.14853451935671202 1105 72-73 -0.501842317375349 1105 74-75 -0.5759880910374271 1105 76-77 -0.6125399968790859 1106 1 0.03215740630125907 1106 2 0.0030454510588597827 1106 3 -0.012749580456478782 1106 4 0.11523416657128394 1106 5 0.18039357423522517 1106 6 0.13030920666434298 1106 7 -0.3141872097517364 1106 8 -0.10731721898720537 1106 9 0.004262311332468016 1106 10-11 -0.19731212174428947 1106 12-13 0.14906235622687092 1106 14-15 0.10145063880125349 1106 16-17 0.1444258644854699 1106 18-19 0.07844770949216695 1106 20-21 0.1259939656960185 1106 22-23 0.032232906760135904 1106 24-25 -0.09430285138115835 1106 26-27 -0.07161953263802445 1106 28-29 0.168600214697598 1106 30-31 0.38345240594478724 1106 32-33 0.26070995869549307 1106 34-35 0.185073136821309 1106 36-37 0.18803356907626068 1106 38-39 0.2951860388066265 1106 40-41 0.3033944365461849 1106 42-43 0.3946065888441055 1106 44-45 0.27112657322476963 1106 46-47 0.1792588009634457 1106 48-49 0.04763381146967305 1106 50-51 0.19686264400719367 1106 52-53 0.2577127691841916 1106 54-55 0.23945923280411918 1106 56-57 0.19037784912204003 1106 58-59 -0.016992964275189593 1106 60-61 -0.14528186585679848 1106 62-63 -0.19589123688119514 1106 64-65 -0.11904995398735707 1106 66-67 -0.1918983558544447 1106 68-69 0.014457751400154706 1106 70-71 -0.19475970546092825 1106 72-73 -0.1285056238269675 1106 74-75 -0.03597762267514426 1106 76-77 -0.20953014886419652 1107 1 0.07967983453092131 1107 2 0.1591563960349376 1107 3 0.0806983860506989 1107 4 0.05685348475310548 1107 5 0.09213998571848236 1107 6 -0.15854097276149304 1107 7 -0.021062209751733008 1107 8 -0.0607457476953428 1107 9 -0.1723719590024544 1107 10-11 -0.08283694231845118 1107 12-13 0.05268376771012839 1107 14-15 -0.1489246602418106 1107 16-17 -0.048331312548022254 1107 18-19 0.10321744633427699 1107 20-21 0.08068370851898266 1107 22-23 0.04088272733429932 1107 24-25 -0.02886325807972412 1107 26-27 -0.2604898974705634 1107 28-29 -0.16164350539809647 1107 30-31 0.04834774087301952 1107 32-33 0.21708630438927656 1107 34-35 0.10321084615146248 1107 36-37 0.1619301001767397 1107 38-39 0.14444067397408134 1107 40-41 0.10835556094809817 1107 42-43 0.054100458461327605 1107 44-45 0.05967770958840646 1107 46-47 0.036194357183539694 1107 48-49 0.12456338682852319 1107 50-51 0.1534206583612594 1107 52-53 0.1558048744473517 1107 54-55 0.19220743854574707 1107 56-57 0.16307596515074962 1107 58-59 0.08385781323677222 1107 60-61 0.152872290841767 1107 62-63 0.18235263273603053 1107 64-65 0.15577959146719067 1107 66-67 0.21944285227952776 1107 68-69 0.14017395953412404 1107 70-71 0.05918168209887753 1107 72-73 0.14233020153188747 1107 74-75 -0.039106360713422106 1107 76-77 0.2226401561597271 1108 1 0.11178999386432764 1108 2 0.23830507928682465 1108 3 0.27752739342301425 1108 4 0.1372691732498268 1108 5 0.20974352971163057 1108 6 0.19697373990863554 1108 7 0.28657729752045213 1108 8 0.14839914714517732 1108 9 0.21468704790118665 1108 10-11 0.18035011111412302 1108 12-13 0.32145420098792954 1108 14-15 0.32842906340795963 1108 16-17 0.2620650007276808 1108 18-19 0.2902878326741174 1108 20-21 0.17989131097651523 1108 22-23 0.1264545786212281 1108 24-25 0.2452643421480829 1108 26-27 0.0990471618558928 1108 28-29 0.22158821187776567 1108 30-31 0.3054212765295361 1108 32-33 0.2661181380513824 1108 34-35 0.20408568691036066 1108 36-37 0.22028739068505132 1108 38-39 0.2899769170345863 1108 40-41 0.1675304932395676 1108 42-43 0.27010279506257007 1108 44-45 0.30188523677477974 1108 46-47 0.23499293015608202 1108 48-49 0.26374676227109717 1108 50-51 0.15622428692794088 1108 52-53 0.34183491225335416 1108 54-55 0.32003006242715415 1108 56-57 0.3682459572401804 1108 58-59 0.4459234241189911 1108 60-61 0.4536463676694673 1108 62-63 0.5064638295331712 1108 64-65 0.5272328821658085 1108 66-67 0.5117419120292013 1108 68-69 0.4196910364995894 1108 70-71 0.42714924378217134 1108 72-73 0.3358601931810483 1108 74-75 0.2246159789128619 1108 76-77 0.31502790805180325 1109 1 -0.27262783186531436 1109 2 -0.2151265456457523 1109 3 -0.35569108734683397 1109 4 -0.22215817165519525 1109 5 -0.3325602903543441 1109 6 -0.5176467175417656 1109 7 -0.4796077460009869 1109 8 -0.3934677956798396 1109 9 -0.31264770214865223 1109 10-11 -0.37065675176226875 1109 12-13 -0.2943315173501233 1109 14-15 -0.18560204993452345 1109 16-17 -0.16881484851632678 1109 18-19 -0.24719526534305913 1109 20-21 -0.14306563211704315 1109 22-23 -0.26950372445616466 1109 24-25 -0.36628345353813785 1109 26-27 -0.406405800612859 1109 28-29 -0.2528435764947332 1109 30-31 -0.23575033521758115 1109 32-33 -0.3020045222811376 1109 34-35 -0.22439351611458136 1109 36-37 -0.23401518766429774 1109 38-39 -0.3109981470831329 1109 40-41 -0.18412091695891064 1109 42-43 -0.17699326511036162 1109 44-45 -0.21776965954935434 1109 46-47 -0.297934035315766 1109 48-49 -0.26677217639731765 1109 50-51 -0.3099367118945402 1109 52-53 -0.3391071828829091 1109 54-55 -0.16864105179744016 1109 56-57 -0.2330211734962937 1109 58-59 -0.25888387949388303 1109 60-61 -0.20670047355601895 1109 62-63 -0.290446453328169 1109 64-65 -0.3074026352336112 1109 66-67 -0.3143322608873973 1109 68-69 -0.3936386012061952 1109 70-71 -0.43257501402893084 1109 72-73 -0.47790365408460644 1109 74-75 -0.5073194628489013 1109 76-77 -0.28801923289655207 1110 1 -0.2581565226784548 1110 2 -0.12062586574594292 1110 3 -0.35001754987631273 1110 4 -0.40833462093943496 1110 5 -0.3427616129611124 1110 6 -0.3251256497231907 1110 7 -0.34420810857997 1110 8 -0.5522642574267635 1110 9 -0.3895165361953943 1110 10-11 -0.42462072777458104 1110 12-13 -0.3729741184801867 1110 14-15 -0.38923071658600605 1110 16-17 -0.4195813518620568 1110 18-19 -0.36874916818717196 1110 20-21 -0.5023318506086412 1110 22-23 -0.38157862867856096 1110 24-25 -0.5173678342334043 1110 26-27 -0.5430740107031795 1110 28-29 -0.39566252108405564 1110 30-31 -0.2627409707342636 1110 32-33 -0.1988705017932162 1110 34-35 -0.34021484724556217 1110 36-37 -0.24094060211550783 1110 38-39 -0.24543302199646888 1110 40-41 -0.1229293345312712 1110 42-43 -0.10251182891811084 1110 44-45 -0.13835533231967645 1110 46-47 -0.13760820568131038 1110 48-49 -0.09151706199417475 1110 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-0.8858516531014828 2314 1 -0.27342713136550145 2314 2 -0.17636788598011321 2314 3 -0.321864967587004 2314 4 -0.20924227535913786 2314 5 -0.27658785396252483 2314 6 -0.17485043563227975 2314 7 -0.2797432194299958 2314 8 -0.23626462612819665 2314 9 -0.10091122711900624 2314 10-11 -0.31650295682145213 2314 12-13 -0.2994422540268502 2314 14-15 -0.2348932828571364 2314 16-17 -0.36669530998484134 2314 18-19 -0.40471325702627325 2314 20-21 -0.356461280511148 2314 22-23 -0.33863952653831575 2314 24-25 -0.28432034418785435 2314 26-27 -0.23253500314547892 2314 28-29 -0.3823699821373552 2314 30-31 -0.4338005694671949 2314 32-33 -0.5386744007735089 2314 34-35 -0.5293303502961351 2314 36-37 -1.2086465577874534 2314 38-39 -0.7795482585383908 2314 40-41 -0.5523286539586536 2314 42-43 -0.4337050692123938 2314 44-45 -0.40055625112783844 2314 46-47 -0.2953055545819403 2314 48-49 -0.2166732199846848 2314 50-51 -0.16066400391328628 2314 52-53 -0.0589096477658444 2314 54-55 -0.11360070049433446 2314 56-57 -0.16330352839561257 2314 58-59 -0.1101705244373683 2314 60-61 -0.07443121682462461 2314 62-63 -0.1150183540012577 2314 64-65 -0.03499603690653075 2314 66-67 0.017511384532685526 2314 68-69 -0.024335980616587705 2314 70-71 0.1195951859855775 2314 72-73 0.08245803364099302 2314 74-75 0.10704262483989524 2314 76-77 -0.013809469750931669 2315 1 -1.0911341877754026 2315 2 -0.8843998551593089 2315 3 -0.7112903537076178 2315 4 -0.6350552163019358 2315 5 -0.851201172481467 2315 6 -0.8325356238100596 2315 7 -1.3714206927321655 2315 8 -1.272380791085844 2315 9 -0.8524824454351503 2315 10-11 -1.035894355059753 2315 12-13 -0.917032692017564 2315 14-15 -0.8991447947286346 2315 16-17 -0.8024921869752646 2315 18-19 -0.82831371505889 2315 20-21 -0.697604474735499 2315 22-23 -0.7792691201763375 2315 24-25 -0.8196283338255412 2315 26-27 -0.7243494894190476 2315 28-29 -0.7773051223434138 2315 30-31 -0.7916817738997608 2315 32-33 -0.8511865251560238 2315 34-35 -0.9804673097747738 2315 36-37 -0.9877840736468713 2315 38-39 -0.9234543642354609 2315 40-41 -1.0828858837432875 2315 42-43 -1.1936991184059593 2315 44-45 -1.0012453612432068 2315 46-47 -0.9769584078947702 2315 48-49 -0.9594119286134166 2315 50-51 -0.8661303538486074 2315 52-53 -0.9564811466560634 2315 54-55 -1.0900115860112116 2315 56-57 -1.2200357883076016 2315 58-59 -1.111095500458518 2315 60-61 -1.2456466725440158 2315 62-63 -1.08879021671234 2315 64-65 -0.9648056160082596 2315 66-67 -1.0163342477064923 2315 68-69 -1.196564678913436 2315 70-71 -1.094742618888965 2315 72-73 -0.9724595738403679 2315 74-75 -0.9517305625706136 2315 76-77 -1.1696200627612754 2316 1 -1.8174722743154845 2316 2 -1.5871605878545054 2316 3 -1.7233764159924547 2316 4 -2.458281343707718 2316 5 -1.8937327106399522 2316 6 -1.9538716854061349 2316 7 -1.820126086403718 2316 8 -1.4932372813954302 2316 9 -1.7616687642916133 2316 10-11 -1.6811828082347446 2316 12-13 -2.1581797509684293 2316 14-15 -3.2578000036363406 2316 16-17 -2.3925987316966157 2316 18-19 -1.947059816205332 2316 20-21 -1.7884845126446578 2316 22-23 -2.076382640229582 2316 24-25 -2.0081914343473883 2316 26-27 -2.0585268839596083 2316 28-29 -2.5654136435990296 2316 30-31 -2.601579715934797 2316 32-33 -2.6503030277215416 2316 34-35 -2.561808975880595 2316 36-37 -2.6502290961219757 2316 38-39 -2.653697266993671 2316 40-41 -2.4610778468605723 2316 42-43 -2.548379139863677 2316 44-45 -2.487836807832501 2316 46-47 -2.4194074527076275 2316 48-49 -2.3063977138607257 2316 50-51 -2.420421680515563 2316 52-53 -2.371997216131515 2316 54-55 -2.4429737701327383 2316 56-57 -2.0963216277502035 2316 58-59 -2.1736371456261416 2316 60-61 -2.0529041584412333 2316 62-63 -1.9933740287314166 2316 64-65 -1.9748988893764903 2316 66-67 -1.886664783901434 2316 68-69 -1.9274057764999952 2316 70-71 -1.892628648192204 2316 72-73 -1.7889337625934836 2316 74-75 -1.9602950327338853 2316 76-77 -2.27317758829151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78499.0 3 6733.0 4 8103.0 5 10192.0 6 11503.0 7 12128.0 8 13866.0 9 14334.0 10 16109.0 11 17559.0 12 15167.0 13 14132.0 14 14309.0 15 15002.0 16 15502.0 17 16107.0 18 17033.0 19 18603.0 20 19861.0 21 21927.0 22 24275.0 23 27665.0 24 32330.0 25 38059.0 26 44676.0 27 52873.0 28 62453.0 29 73430.0 30 88331.0 31 106913.0 32 132907.0 33 168039.0 34 225247.0 35 304234.0 36 432640.0 37 557435.0 38 457864.0 39 111745.0 40 711.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 23.608273458397896 25.740396185669244 27.36603137942817 23.28529897650469 2 23.1649326126266 26.11037873486619 27.439358640537208 23.285330011970007 3 23.274948023844903 26.034618832938616 26.876303572885018 23.814129570331463 4 23.272071543210682 26.25567667892792 27.158244749801618 23.314007028059784 5 23.39527262627696 26.420195096038047 26.997715025887885 23.186817251797113 6 23.473479179451854 26.085750289258502 26.92663143008919 23.514139101200453 7 23.422028352003416 26.000434206760044 26.909236232734823 23.668301208501717 8 23.24486276707259 26.05092341172486 26.704605581738623 23.99960823946393 9 23.074406377256313 25.961879580469198 27.055188494740705 23.908525547533785 10-11 23.76389373001252 26.082589375396754 26.814370169857867 23.33914672473286 12-13 23.863176871935423 25.765105196136833 26.855614248436904 23.51610368349084 14-15 23.542181426956073 25.961821973626385 26.856551722674254 23.639444876743287 16-17 23.788547791461998 25.92287387126403 26.951365761616238 23.33721257565773 18-19 23.664776452699297 25.890205881973937 26.71826665282043 23.726751012506337 20-21 23.480688571079025 26.02833364689167 26.90206231437586 23.588915467653436 22-23 23.848916805941982 26.186831872660722 26.454158611338187 23.510092710059112 24-25 23.6681666171636 26.268571041297385 26.347017860979317 23.7162444805597 26-27 23.731152624927535 26.337417739818903 26.45102957121615 23.48040006403741 28-29 24.018997300999498 26.093819828527664 26.375347228749234 23.5118356417236 30-31 23.629937368348845 26.12489492541884 26.55124190764655 23.693925798585767 32-33 23.536662588186214 26.32273822840936 26.446987611899864 23.693611571504565 34-35 23.562277603809147 26.02981412711542 26.581817542344723 23.826090726730715 36-37 23.348530982359357 26.21305663758269 26.74919634377158 23.689216036286375 38-39 23.486543498978318 26.175236059947903 26.82778365171345 23.51043678936033 40-41 23.326508753830772 26.182068461574033 26.61557201775856 23.87585076683664 42-43 23.285505762837673 26.26167819072488 26.557445843394902 23.895370203042546 44-45 23.54431865146639 25.945072095376393 26.759524100471722 23.751085152685494 46-47 23.48627647595785 25.886056825588238 26.552512554319424 24.075154144134487 48-49 23.491237802107573 26.058580810928365 26.494807022129223 23.955374364834835 50-51 23.375225864714825 26.36450186794687 26.407290504364607 23.852981762973698 52-53 23.55835460087852 25.998571330444353 26.548886594539727 23.894187474137397 54-55 23.49266675288873 26.133715257651087 26.570764079605507 23.802853909854683 56-57 23.610280017204214 26.154374907804794 26.398626816406583 23.836718258584405 58-59 23.615034378536247 26.141923500024866 26.501218318562714 23.741823802876176 60-61 23.430972810123887 26.2009479773799 26.468363532935797 23.89971567956042 62-63 23.492982415973895 26.20946753331622 26.450879641008278 23.846670409701606 64-65 23.445643833464757 26.099186542449875 26.284232101864358 24.17093752222101 66-67 23.289129639136416 26.17256330584968 26.449532557532372 24.088774497481534 68-69 23.427128619672384 25.951240562650412 26.625683540680072 23.99594727699713 70-71 23.238977232138456 25.77731737669462 26.80645535571419 24.177250035452737 72-73 23.382166297836942 25.53058617001541 26.841535793927406 24.24571173822024 74-75 23.189289651607837 25.69249027675945 26.637985060260043 24.480235011372667 76-77 23.18957873331002 25.5115023654012 26.89136145014226 24.40755745114652 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4290.0 1 2627.0 2 922.0 3 910.0 4 940.0 5 972.0 6 1071.5 7 1208.5 8 1329.0 9 1380.0 10 1455.5 11 1593.0 12 1746.5 13 1838.0 14 1981.0 15 2291.0 16 2717.0 17 2976.0 18 3312.0 19 4051.0 20 5007.5 21 6350.0 22 7139.0 23 9024.0 24 12396.0 25 16315.5 26 18748.0 27 20566.0 28 27936.5 29 35071.5 30 36654.0 31 41847.5 32 51764.0 33 62163.5 34 75591.5 35 83343.0 36 87944.5 37 97471.0 38 105729.0 39 109062.0 40 114671.5 41 124141.5 42 133343.0 43 138684.0 44 140780.0 45 147938.0 46 155728.0 47 161546.5 48 164637.0 49 165221.5 50 165183.5 51 162082.5 52 159604.0 53 158646.5 54 154012.5 55 142159.0 56 129241.5 57 124501.0 58 118925.0 59 103181.5 60 84144.5 61 75275.0 62 66711.5 63 50966.0 64 37725.0 65 31666.0 66 26764.5 67 19016.5 68 14195.0 69 10343.5 70 8467.0 71 7162.0 72 5049.0 73 3824.5 74 3408.0 75 3039.5 76 2141.5 77 1372.0 78 1132.0 79 1015.0 80 762.5 81 512.5 82 344.5 83 291.0 84 237.0 85 157.5 86 121.0 87 110.0 88 96.0 89 79.5 90 71.5 91 66.0 92 54.0 93 39.0 94 31.5 95 29.5 96 32.0 97 32.5 98 33.0 99 64.5 100 96.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002819466811540775 2 0.007639845553852423 3 0.010580579755136887 4 0.017371553580783485 5 0.01297561070257475 6 0.012369273753856302 7 0.01600729544616698 8 0.01706838510642426 9 0.015946661751295135 10-11 0.01972110925706746 12-13 0.019175406003220864 14-15 0.007200251266031549 16-17 0.005199339335260676 18-19 0.011171758280137372 20-21 0.010080351772444169 22-23 0.01430955198975533 24-25 0.021843288577582023 26-27 0.012854343312831059 28-29 0.00721540968974951 30-31 0.007382152350647082 32-33 0.008807044180135433 34-35 0.009943925958982518 36-37 0.008746410485263586 38-39 0.015128106870525234 40-41 0.011171758280137372 42-43 0.014188284600011642 44-45 0.017371553580783485 46-47 0.01599213702244902 48-49 0.013233303905780088 50-51 0.02702746948912474 52-53 0.030998976503230562 54-55 0.02454148799937911 56-57 0.014142809328857758 58-59 0.015400958497448533 60-61 0.019175406003220864 62-63 0.024723389083994643 64-65 0.019993960883990764 66-67 0.01991816876540096 68-69 0.02910417353848542 70-71 0.056192276722482 72-73 0.06850091678146646 74-75 0.0805063883660917 76-77 0.07711090145326839 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 3298496.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.529847838827955 #Duplication Level Percentage of deduplicated Percentage of total 1 71.12389545730088 34.51631824247519 2 14.024606556640649 13.61224044386399 3 5.6281768166000985 8.194036935588658 4 2.8786798503181013 5.5880758045054995 5 1.7090758626817841 4.14705957804803 6 1.097633163718771 3.196078225087398 7 0.7112162411193602 2.4160651173418013 8 0.4963831574923911 1.9271519282290168 9 0.38372795863833475 1.6760033499800204 >10 1.7946712921045636 15.313473513088969 >50 0.10207696538463515 3.3823582751173413 >100 0.04578431203881826 3.73335546914117 >500 0.0021928124424035324 0.717614454658453 >1k 0.0018795535193574335 1.5801686628745033 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4555 0.13809324007062612 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.031684743592231E-5 3.031684743592231E-5 2 0.0 0.0 0.0 1.8190108461553386E-4 3.031684743592231E-5 3 0.0 0.0 0.0 4.547527115388347E-4 6.063369487184462E-5 4 0.0 0.0 0.0 0.0010914065076932033 6.063369487184462E-5 5 0.0 0.0 0.0 0.002546615184617474 6.063369487184462E-5 6 0.0 0.0 0.0 0.0051841809115427155 6.063369487184462E-5 7 0.0 0.0 0.0 0.01103533246667572 6.063369487184462E-5 8 0.0 0.0 0.0 0.017371553580783485 9.095054230776693E-5 9 0.0 0.0 0.0 0.024435379033353383 9.095054230776693E-5 10 0.0 0.0 0.0 0.03225712567182134 9.095054230776693E-5 11 0.0 0.0 0.0 0.04168566522439318 9.095054230776693E-5 12 0.0 0.0 0.0 0.05387303789363395 9.095054230776693E-5 13 0.0 0.0 0.0 0.07154775994877666 9.095054230776693E-5 14 0.0 0.0 0.0 0.0907686412231514 1.5158423717961155E-4 15 0.0 0.0 0.0 0.11417324744368343 1.5158423717961155E-4 16 0.0 0.0 0.0 0.13390951512446886 1.5158423717961155E-4 17 0.0 0.0 0.0 0.15573764527833292 1.5158423717961155E-4 18 0.0 0.0 0.0 0.17792957760142805 1.5158423717961155E-4 19 0.0 0.0 0.0 0.20172830283862705 2.1221793205145617E-4 20 0.0 0.0 0.0 0.22943790139506004 2.1221793205145617E-4 21 0.0 0.0 0.0 0.26033076893226487 3.031684743592231E-4 22 0.0 0.0 0.0 0.2933761326374202 3.031684743592231E-4 23 0.0 0.0 0.0 0.32630022895283184 3.6380216923106773E-4 24 0.0 0.0 0.0 0.3591030578784998 3.9411901666699007E-4 25 0.0 0.0 0.0 0.3940280661246823 4.2443586410291235E-4 26 0.0 0.0 0.0 0.4316512737926619 4.547527115388347E-4 27 0.0 0.0 0.0 0.4700324026465395 4.547527115388347E-4 28 0.0 0.0 0.0 0.5066854711965696 4.8506955897475697E-4 29 0.0 0.0 0.0 0.5444602631017288 5.153864064106793E-4 30 0.0 0.0 0.0 0.586145928326122 5.457032538466016E-4 31 0.0 0.0 0.0 0.6321365858864161 5.457032538466016E-4 32 0.0 0.0 0.0 0.6828566716467142 5.457032538466016E-4 33 0.0 0.0 0.0 0.7373360464890665 5.457032538466016E-4 34 0.0 0.0 0.0 0.7920276392634704 5.457032538466016E-4 35 0.0 0.0 0.0 0.8471436679019771 5.457032538466016E-4 36 0.0 0.0 0.0 0.9048366285725373 5.457032538466016E-4 37 0.0 0.0 0.0 0.9677137701546402 5.457032538466016E-4 38 0.0 0.0 0.0 1.0280442965521255 6.063369487184462E-4 39 0.0 0.0 0.0 1.09261918159064 6.063369487184462E-4 40 0.0 0.0 0.0 1.1612868410330042 6.366537961543685E-4 41 0.0 0.0 0.0 1.2364726226740914 6.366537961543685E-4 42 0.0 0.0 0.0 1.3168729020741574 6.366537961543685E-4 43 0.0 0.0 0.0 1.4043066900793575 6.366537961543685E-4 44 0.0 0.0 0.0 1.499259056248666 6.366537961543685E-4 45 0.0 0.0 0.0 1.595242195230796 6.366537961543685E-4 46 0.0 0.0 0.0 1.6973796542424184 6.669706435902909E-4 47 0.0 0.0 0.0 1.8017908768117348 6.972874910262132E-4 48 0.0 0.0 0.0 1.913690360697724 7.276043384621355E-4 49 3.031684743592231E-5 0.0 0.0 2.0282577271580746 7.882380333339801E-4 50 3.031684743592231E-5 0.0 0.0 2.1425219251440657 7.882380333339801E-4 51 3.031684743592231E-5 0.0 0.0 2.2619399871941637 8.488717282058247E-4 52 3.031684743592231E-5 0.0 0.0 2.3978504142494033 8.79188575641747E-4 53 3.031684743592231E-5 0.0 0.0 2.5433409650943943 8.79188575641747E-4 54 3.031684743592231E-5 0.0 0.0 2.694591716952211 8.79188575641747E-4 55 3.031684743592231E-5 0.0 0.0 2.8512995013484934 8.79188575641747E-4 56 3.031684743592231E-5 0.0 0.0 3.0153136459768333 9.095054230776694E-4 57 3.031684743592231E-5 0.0 0.0 3.1817228215526105 9.701391179495139E-4 58 6.063369487184462E-5 0.0 0.0 3.3654429170143 0.0010004559653854362 59 6.063369487184462E-5 0.0 0.0 3.544433584275985 0.0010004559653854362 60 6.063369487184462E-5 0.0 0.0 3.731912968819729 0.0010004559653854362 61 6.063369487184462E-5 0.0 0.0 3.9318222608122007 0.0010004559653854362 62 6.063369487184462E-5 0.0 0.0 4.132155988668775 0.0010004559653854362 63 6.063369487184462E-5 0.0 0.0 4.346283882108694 0.0010307728128213585 64 6.063369487184462E-5 0.0 0.0 4.5683244727293895 0.0010307728128213585 65 6.063369487184462E-5 0.0 0.0 4.801946099070607 0.0010307728128213585 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGTA 205 2.7584974E-8 19.04953 57 AATTCGC 195 3.4089499E-7 18.204739 55 ATTCGCG 285 2.1827873E-10 17.437431 56 CGGTTAC 245 1.1192242E-8 17.384748 4 TCGAACT 660 0.0 17.211493 24 CGCGTTG 135 0.002684159 15.776007 10 TAAGGCG 1470 0.0 15.212577 17 CGAACTC 760 0.0 14.945463 25 CTCGAAC 825 0.0 14.199482 23 CCTCGAA 830 0.0 14.113942 22 ACGACGG 180 0.0011324979 13.805052 24 CGATCGT 285 1.1725697E-6 13.698553 1 TACGACG 160 0.008288809 13.312015 23 CGCTCCG 400 1.8353603E-9 13.310802 25 TAGCGCG 160 0.008295457 13.310399 5 CCGGGGT 1230 0.0 13.278355 48 CAATTCG 295 1.7236143E-6 13.237407 54 GCCTCGA 840 0.0 13.100713 21 GACGCGA 1125 0.0 12.944176 69 GGCGGAA 1760 0.0 12.908619 20 >>END_MODULE