FastQCFastQC Report
Thu 26 May 2016
SRR936008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298496
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52400.1588602805642329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37390.11335469256291353No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67850.036.841191
CGTATCA13700.035.191532100-101
TAAATCG3150.034.0081596-97
TACGCAG7800.032.05003100-101
GCGTACA5950.030.511011100-101
GTATCAA127150.030.0505811
CGTACAT20100.029.464615100-101
CCGTACA9350.029.283298100-101
TCGAGTA5250.028.910433100-101
TCGTACA8550.028.890543100-101
CCTGTCG11250.028.3023496-97
CGGTATC12450.026.772713100-101
TTATACG7900.026.7437596-97
ACGTATC5400.025.902868100-101
ACCGTAC3400.025.38243198-99
AGGTATC21350.025.091022100-101
CGAGTAC16550.024.276133100-101
TCGTATC6350.023.902327100-101
TCGTATA3200.023.25058100-101
ATCAACG165100.022.991273
Produced by FastQC (version 0.11.5)