FastQCFastQC Report
Thu 26 May 2016
SRR936007_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936007_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676557
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106000.6322481132463734No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37680.22474631044455987No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGA351.92164E-550.70633725
CGCGTCA501.5762227E-435.491262
TCGCGAT552.7579875E-432.26670526
CGCGGAA1655.8591468E-821.51434759
CGGTTAC1858.4928615E-921.1035424
CGCTATT1054.9726595E-420.28374517
CGGTATA1257.027263E-519.87747810
GCACGAC2052.7614078E-819.04636424
GGGCGTA2305.113179E-918.51883734
CGTGCGA1602.3058157E-517.75304452
ACGCACG1602.3064073E-517.75251449
GCCGTAA1401.6764448E-417.74721739
ACGTTAT1000.008944686517.745632
CGTAGGG2651.5570549E-917.4175649
CGGGTTA2651.5643309E-917.41132528
TCCGCGA2902.7830538E-1017.1413851
TACGCAC1452.1872943E-417.14035848
TACCGCG1250.001605409917.0368215
AATTCGC1905.3508793E-616.81616455
CGGCGTA2752.5484042E-916.78369546
Produced by FastQC (version 0.11.5)