##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936007_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1676557 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.11355712928341 40.0 38.0 41.0 34.0 41.0 2 37.990028373625236 40.0 38.0 41.0 34.0 41.0 3 37.90796793667021 40.0 38.0 41.0 33.0 41.0 4 37.79686166351636 40.0 38.0 41.0 33.0 41.0 5 37.71991408583186 40.0 38.0 41.0 33.0 41.0 6 37.58431654873649 40.0 37.0 41.0 33.0 41.0 7 37.3971054965623 40.0 37.0 41.0 33.0 41.0 8 37.195314564312454 40.0 36.0 41.0 32.0 41.0 9 37.08427330535138 39.0 36.0 41.0 32.0 41.0 10-11 37.25318494987047 40.0 37.0 41.0 33.0 41.0 12-13 37.41128694103451 40.0 37.0 41.0 33.0 41.0 14-15 37.33673385396381 40.0 37.0 41.0 33.0 41.0 16-17 37.14154901980666 40.0 37.0 41.0 32.0 41.0 18-19 36.93191910564329 40.0 37.0 41.0 31.5 41.0 20-21 36.634820050854216 40.0 36.0 41.0 31.0 41.0 22-23 36.23011177073013 40.0 36.0 41.0 30.0 41.0 24-25 35.88667220977277 40.0 35.5 41.0 28.5 41.0 26-27 34.92039071740478 38.5 34.5 40.0 27.0 40.5 28-29 34.93390859958832 38.5 35.0 40.0 26.5 40.5 30-31 35.28066537552854 39.0 35.0 40.5 27.0 41.0 32-33 35.11675296455772 39.0 35.0 41.0 26.5 41.0 34-35 34.8523709602477 38.5 35.0 40.5 26.0 41.0 36-37 34.561439605095444 38.0 34.5 40.0 26.0 41.0 38-39 34.27886257371506 37.5 34.0 40.0 25.0 41.0 40-41 33.9116779208819 37.0 34.0 40.0 23.5 41.0 42-43 33.554007111001894 36.5 34.0 39.5 22.5 41.0 44-45 33.188080095099664 36.0 33.5 39.0 22.0 41.0 46-47 32.80184538909205 35.5 33.0 39.0 20.5 40.5 48-49 32.364711429435445 35.0 33.0 38.0 18.5 40.0 50-51 31.950783063146677 35.0 32.5 37.0 17.5 39.5 52-53 31.531576021572782 35.0 32.0 37.0 12.5 39.0 54-55 31.104137527086763 35.0 32.0 36.0 7.5 39.0 56-57 30.686947714870417 35.0 31.0 36.0 4.5 37.5 58-59 30.288579213232833 35.0 31.0 35.0 2.0 37.0 60-61 29.965932562984737 34.0 31.0 35.0 2.0 37.0 62-63 29.64474246923904 34.0 30.5 35.0 2.0 36.0 64-65 29.301834652803336 34.0 30.0 35.0 2.0 36.0 66-67 28.96673092534283 34.0 30.0 35.0 2.0 35.5 68-69 28.648280672831284 34.0 29.0 35.0 2.0 35.0 70-71 28.278793086068653 34.0 29.0 35.0 2.0 35.0 72-73 27.86111924616938 34.0 29.0 35.0 2.0 35.0 74-75 27.356592409324584 34.0 28.0 35.0 2.0 35.0 76-77 25.710048927653517 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.43182724697715 1101 2 0.40022207472635785 1101 3 0.4492819153379486 1101 4 0.4384018945248158 1101 5 0.5810502146678616 1101 6 0.5014835355744793 1101 7 0.451741235236895 1101 8 0.6552815755031034 1101 9 0.6313269448357346 1101 10-11 0.5851418801067965 1101 12-13 0.6953513455535472 1101 14-15 0.6592840350901312 1101 16-17 0.6078166140606598 1101 18-19 0.70478794245507 1101 20-21 0.5842871965486651 1101 22-23 0.6641671242159646 1101 24-25 0.6604048561665437 1101 26-27 0.43460046757395787 1101 28-29 0.6128431568788031 1101 30-31 0.723059384782907 1101 32-33 0.7560866444435064 1101 34-35 0.7480225956529694 1101 36-37 0.6850425116304351 1101 38-39 0.7331702241132589 1101 40-41 0.7661929836117309 1101 42-43 0.7566285683430323 1101 44-45 0.5595704357724074 1101 46-47 0.6023308730368555 1101 48-49 0.711078485098362 1101 50-51 0.6507723757166879 1101 52-53 0.6383429891967438 1101 54-55 0.627281068899034 1101 56-57 0.5564751284940641 1101 58-59 0.5436666416908764 1101 60-61 0.5411163046743575 1101 62-63 0.44333497029229463 1101 64-65 0.4537376391680361 1101 66-67 0.30422575264283935 1101 68-69 0.43913593368853654 1101 70-71 0.4807164729687514 1101 72-73 0.37648267649136 1101 74-75 0.5023019689524695 1101 76-77 0.4136275764760384 1102 1 0.591449788178636 1102 2 0.5339009689315688 1102 3 0.5082266255984464 1102 4 0.5540983325896747 1102 5 0.6815951375812048 1102 6 0.7746228231874497 1102 7 0.593142085848271 1102 8 0.7206457434988494 1102 9 0.8097426811462114 1102 10-11 0.5753997216804194 1102 12-13 0.7301599579937417 1102 14-15 0.6739770175462638 1102 16-17 0.746326715070758 1102 18-19 0.6628554597331089 1102 20-21 0.8236877813439847 1102 22-23 0.6956530359650301 1102 24-25 0.6162395185801515 1102 26-27 0.505752780173637 1102 28-29 0.49609009999415576 1102 30-31 0.6712053177972663 1102 32-33 0.5726602754908257 1102 34-35 0.7150547593956631 1102 36-37 0.6388170996155509 1102 38-39 0.5036978689830178 1102 40-41 0.5553875609641992 1102 42-43 0.5668757772744542 1102 44-45 0.5818657574098367 1102 46-47 0.5642527231166028 1102 48-49 0.5559947530409417 1102 50-51 0.6868303236167428 1102 52-53 0.6764344299197589 1102 54-55 0.7496229083461365 1102 56-57 0.8390708222739676 1102 58-59 0.8182413360024121 1102 60-61 0.811690467353781 1102 62-63 0.8722225301009097 1102 64-65 0.9038838379984426 1102 66-67 0.8860705160665496 1102 68-69 0.9609793680319711 1102 70-71 0.9436683602627411 1102 72-73 0.8733526924403243 1102 74-75 0.7792690077616165 1102 76-77 1.1684122654712539 1103 1 0.2604071039715521 1103 2 0.214678791455313 1103 3 0.3201576204388985 1103 4 0.4010223918057889 1103 5 0.4628252385349825 1103 6 0.4078501430075079 1103 7 0.2646416607616189 1103 8 0.3433932144914138 1103 9 0.36757202004181266 1103 10-11 0.41945935083964514 1103 12-13 0.458844013630177 1103 14-15 0.5292597948249593 1103 16-17 0.6287114339800652 1103 18-19 0.6066726001906915 1103 20-21 0.6730023076047047 1103 22-23 0.7948818564958557 1103 24-25 0.7662161129993024 1103 26-27 0.5422427691248259 1103 28-29 0.595882891763658 1103 30-31 0.8914463264401107 1103 32-33 0.846227974412443 1103 34-35 0.8751310340532683 1103 36-37 0.7396531607316561 1103 38-39 0.670443873684782 1103 40-41 0.8319687284208044 1103 42-43 0.7293774552480841 1103 44-45 0.6947641255589687 1103 46-47 0.3993388935161093 1103 48-49 0.5123979004031725 1103 50-51 0.4986131404784011 1103 52-53 0.5887190899709083 1103 54-55 0.6193123369401796 1103 56-57 0.6739737063234088 1103 58-59 0.569178763935593 1103 60-61 0.4891028771304313 1103 62-63 0.29922138391584596 1103 64-65 0.4362467002256203 1103 66-67 0.4447305809826183 1103 68-69 0.3862334734741104 1103 70-71 0.19861537071609092 1103 72-73 0.29609465623739695 1103 74-75 0.19576822126525784 1103 76-77 0.14737822067710127 1104 1 0.4339010840712305 1104 2 0.5518047026247785 1104 3 0.49410156936812655 1104 4 0.39737318639084407 1104 5 0.4980662957527713 1104 6 0.2616085320355097 1104 7 0.3640165105688524 1104 8 0.3409232617520743 1104 9 0.34153616881014415 1104 10-11 0.20052861887409534 1104 12-13 0.3551833152033268 1104 14-15 0.28742557978025474 1104 16-17 0.28395085431433387 1104 18-19 0.35125817262975545 1104 20-21 0.3876311703885946 1104 22-23 0.25029506510099253 1104 24-25 -0.14136109043745648 1104 26-27 -0.302131575686424 1104 28-29 -0.35730102080587045 1104 30-31 -0.18676727641015134 1104 32-33 -0.24375551744318358 1104 34-35 -0.1530790860661213 1104 36-37 -0.23762672196996704 1104 38-39 -0.2137023870812058 1104 40-41 -0.28429893337816026 1104 42-43 -0.4029570889146896 1104 44-45 -0.4219111898893786 1104 46-47 -0.5446645404565302 1104 48-49 -0.43421079040346555 1104 50-51 -0.4571244845081033 1104 52-53 -0.5625686497715776 1104 54-55 -0.45330625875307007 1104 56-57 -0.4840061008029579 1104 58-59 -0.4622130049782456 1104 60-61 -0.5881226749694797 1104 62-63 -0.6570985536736167 1104 64-65 -0.7135077672474139 1104 66-67 -0.687502963984663 1104 68-69 -0.6802452418161202 1104 70-71 -0.8199895517757341 1104 72-73 -0.6893276771226375 1104 74-75 -0.718033667071424 1104 76-77 -0.5251722876274663 1105 1 0.044771467727649394 1105 2 0.23592142623729728 1105 3 0.06457899110605325 1105 4 0.23338759215314298 1105 5 0.18834169585363725 1105 6 -0.0996791783149007 1105 7 0.026221984474389615 1105 8 0.2951906491804195 1105 9 0.0768544381297076 1105 10-11 0.1658734550334131 1105 12-13 0.1110944370485214 1105 14-15 0.03202128788598202 1105 16-17 0.04986005823258921 1105 18-19 0.22568936522513638 1105 20-21 0.23031534465001613 1105 22-23 0.19706904096351963 1105 24-25 0.19921727108467024 1105 26-27 -0.036572571019469535 1105 28-29 0.22489671035760495 1105 30-31 0.11823886805947126 1105 32-33 0.1910072634087996 1105 34-35 0.18526986298095238 1105 36-37 0.18190345696550736 1105 38-39 0.22921158324064805 1105 40-41 0.14341013841623607 1105 42-43 0.17183460290996777 1105 44-45 0.1558497369665659 1105 46-47 0.02418406407403495 1105 48-49 -0.011702852316993528 1105 50-51 0.026191682195261023 1105 52-53 -0.13455803724254167 1105 54-55 -0.011235821110560096 1105 56-57 0.04465305516712448 1105 58-59 0.11108092797955393 1105 60-61 -0.08915302793907287 1105 62-63 -0.11464168927105334 1105 64-65 -0.1528760589427911 1105 66-67 0.034722257464864015 1105 68-69 -0.3418256136098101 1105 70-71 -0.6246870520776326 1105 72-73 -0.6128691647235947 1105 74-75 -0.8233548487985551 1105 76-77 -0.6325765511080093 1106 1 -0.012964126510041751 1106 2 0.15216292283594157 1106 3 -0.2297012209787752 1106 4 -0.0195385783150428 1106 5 -0.24514419850410007 1106 6 -0.018684280355721228 1106 7 0.08280711852800948 1106 8 -0.040231019487045216 1106 9 -0.06822859508358192 1106 10-11 -0.26637444412624944 1106 12-13 -0.1761642164128645 1106 14-15 -0.15819855004919248 1106 16-17 -0.04100128630522448 1106 18-19 -0.10037230944473663 1106 20-21 0.018142975702424735 1106 22-23 0.12858539750614284 1106 24-25 0.10009637272532501 1106 26-27 0.01089016407456711 1106 28-29 0.19691676838081662 1106 30-31 0.11253658714710468 1106 32-33 -0.08806061374206564 1106 34-35 0.00937450483769453 1106 36-37 0.16173914123921662 1106 38-39 0.10165431033148309 1106 40-41 0.06171496034500734 1106 42-43 0.06916228398240065 1106 44-45 -0.10443562717908605 1106 46-47 0.1635335538699536 1106 48-49 -0.06109761022015192 1106 50-51 -0.11730946826492428 1106 52-53 -0.2040921008679959 1106 54-55 0.10749292038603642 1106 56-57 -0.03225270713779693 1106 58-59 -0.20413890995562056 1106 60-61 -0.16488332005591744 1106 62-63 -0.28813608703015703 1106 64-65 -0.14018973441298144 1106 66-67 -0.232816007841258 1106 68-69 -0.29873962921605113 1106 70-71 -0.41982385679952117 1106 72-73 -0.43585460890126626 1106 74-75 -0.5522088904151978 1106 76-77 -0.6026145663140916 1107 1 -0.09453396675915116 1107 2 -0.09634082273593947 1107 3 -0.04262499520653762 1107 4 0.03569309433100187 1107 5 -0.09801979926224647 1107 6 -0.16542791739626495 1107 7 -0.10230606656190844 1107 8 0.02698831646367239 1107 9 -0.12859068583472322 1107 10-11 -0.03740520971211936 1107 12-13 -0.0312930127530322 1107 14-15 0.04001884824499058 1107 16-17 -0.054949874946665034 1107 18-19 -0.04811163964171783 1107 20-21 -0.10536288521500836 1107 22-23 0.052279594690190834 1107 24-25 -0.16167587788953597 1107 26-27 -0.14611466173377607 1107 28-29 -0.10147987019406202 1107 30-31 0.086373993225763 1107 32-33 0.19028960004886386 1107 34-35 0.2158054373948417 1107 36-37 0.18103003509963855 1107 38-39 0.13854769973557524 1107 40-41 0.025467103895174148 1107 42-43 0.14496816013033254 1107 44-45 0.07630549119000563 1107 46-47 0.12455090816495584 1107 48-49 0.3688407345078488 1107 50-51 0.34010827299728774 1107 52-53 0.20461698834881759 1107 54-55 0.09820197011640275 1107 56-57 0.12675781882172288 1107 58-59 0.23846859190588177 1107 60-61 0.22829454948085015 1107 62-63 0.2071325900609331 1107 64-65 0.19700727759090242 1107 66-67 0.3778281994964523 1107 68-69 0.27452219133495603 1107 70-71 0.3196836766511453 1107 72-73 0.4028703877141808 1107 74-75 0.5650101634894469 1107 76-77 0.3858582105987445 1108 1 0.142001425002654 1108 2 0.08861851882004146 1108 3 0.01244679802263704 1108 4 -0.06494067386116598 1108 5 -0.12373433730876826 1108 6 -0.13523429383189978 1108 7 -0.23366203786306983 1108 8 -0.1504219601244685 1108 9 -0.04988835520756396 1108 10-11 -0.2173506249765964 1108 12-13 -0.04820261510567292 1108 14-15 0.08382207693012589 1108 16-17 -0.02781568560446601 1108 18-19 -0.07736278346939685 1108 20-21 -0.03388139933968404 1108 22-23 -0.020281129345143256 1108 24-25 -0.05150329205468296 1108 26-27 -0.06739486557302854 1108 28-29 -0.14788711107474484 1108 30-31 -0.10247587832018468 1108 32-33 -0.008876470153360572 1108 34-35 0.06211269633422489 1108 36-37 0.07984923321415494 1108 38-39 -0.05682241798871246 1108 40-41 -0.10015292254698238 1108 42-43 -0.13877623936508598 1108 44-45 -0.07804863337813117 1108 46-47 -0.05428890586996005 1108 48-49 0.0408202187671165 1108 50-51 0.01444230890642828 1108 52-53 -0.021132942118583742 1108 54-55 0.06016731058885583 1108 56-57 0.07847517508038138 1108 58-59 -0.05103580874949998 1108 60-61 -0.09126968216089537 1108 62-63 -0.1091092357398118 1108 64-65 -0.26965266649302677 1108 66-67 -0.39943319165636737 1108 68-69 -0.4930840551307476 1108 70-71 -0.39685433823608207 1108 72-73 -0.3212786453543366 1108 74-75 -0.49118074204244877 1108 76-77 -0.562295517732462 1109 1 -0.1178939491112061 1109 2 0.01752875063988313 1109 3 -0.31609040113944786 1109 4 0.011516961960680305 1109 5 -0.23856372440914697 1109 6 -0.16246092456103156 1109 7 -0.2519712331984181 1109 8 -0.03441764065802033 1109 9 -0.07213139816857961 1109 10-11 -0.22081500176368962 1109 12-13 -0.2766427735575192 1109 14-15 -0.1729028208574519 1109 16-17 0.016414827422558176 1109 18-19 -0.23813520609959937 1109 20-21 -0.15086114698697628 1109 22-23 -0.09331087488358492 1109 24-25 -0.09093917723399159 1109 26-27 -0.16251651961001556 1109 28-29 -0.1879621795455222 1109 30-31 -0.12653069376876402 1109 32-33 -0.18605560147999967 1109 34-35 -0.07982699984366803 1109 36-37 -0.018497626904625974 1109 38-39 -0.022166934268049943 1109 40-41 -0.02260571269335543 1109 42-43 0.06506448399156284 1109 44-45 -0.13277822152249286 1109 46-47 -0.17791354878759336 1109 48-49 0.06890963814968387 1109 50-51 0.076303836934418 1109 52-53 0.046580987625148396 1109 54-55 0.03990491572186983 1109 56-57 0.05882535178887238 1109 58-59 0.06016032595733023 1109 60-61 -0.018973348820200187 1109 62-63 -0.02871105752098302 1109 64-65 0.026805866428176728 1109 66-67 0.11176716075639703 1109 68-69 0.14532096916122939 1109 70-71 0.06658133137159794 1109 72-73 -0.07591879135391011 1109 74-75 -0.2228367338962478 1109 76-77 -0.16463396787951012 1110 1 -0.35833394726381584 1110 2 -0.396247075046098 1110 3 -0.4490873054296145 1110 4 -0.4836956026938637 1110 5 -0.44074643740989927 1110 6 -0.4983529172817782 1110 7 -0.6379298615966107 1110 8 -0.2490865272956313 1110 9 -0.273509698428839 1110 10-11 -0.39978288140679297 1110 12-13 -0.39339559091263965 1110 14-15 -0.46363456399428316 1110 16-17 -0.5283081618695533 1110 18-19 -0.42620447929391503 1110 20-21 -0.5493694115719805 1110 22-23 -0.5874312856025767 1110 24-25 -0.6118188545651364 1110 26-27 -0.5053500194633145 1110 28-29 -0.40513733850924183 1110 30-31 -0.5157699372818811 1110 32-33 -0.33324103738963373 1110 34-35 -0.09852785167056766 1110 36-37 -0.20405348408265667 1110 38-39 -0.08412432531265068 1110 40-41 -0.13499224015884437 1110 42-43 -0.08227545498470334 1110 44-45 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48-49 0.010938931669336682 2313 50-51 0.12559838702595627 2313 52-53 -0.07140037748442296 2313 54-55 0.008363423545979742 2313 56-57 0.14843721345854277 2313 58-59 0.12345133068609115 2313 60-61 0.2125676603362372 2313 62-63 0.189179177918799 2313 64-65 0.21824032149368122 2313 66-67 0.23402131593122633 2313 68-69 0.3060980186530138 2313 70-71 0.1433542140155808 2313 72-73 0.2051056284994779 2313 74-75 0.10499516889447236 2313 76-77 -0.05716407206680785 2314 1 -0.6762586661802175 2314 2 -0.7836002897338545 2314 3 -0.6691774253678204 2314 4 -0.48474601890717395 2314 5 -0.544654814008581 2314 6 -0.6192678084689547 2314 7 -0.7688152357116991 2314 8 -0.7684116129164735 2314 9 -0.5668732516216934 2314 10-11 -0.6343055569687337 2314 12-13 -0.5759803928834515 2314 14-15 -0.6542035331682641 2314 16-17 -1.1806503578543754 2314 18-19 -0.8717433164568149 2314 20-21 -0.8811842220640926 2314 22-23 -0.6299869469945705 2314 24-25 -0.7828997479083668 2314 26-27 -0.6753701430284096 2314 28-29 -0.7377244308140973 2314 30-31 -0.8282340919854718 2314 32-33 -1.0008012480752697 2314 34-35 -0.9705712814238296 2314 36-37 -1.0076402413085006 2314 38-39 -0.8492144891750755 2314 40-41 -0.6551765147880886 2314 42-43 -0.6453816875797287 2314 44-45 -0.7644899220758532 2314 46-47 -0.6313003151080991 2314 48-49 -0.721084949526734 2314 50-51 -0.7024100233876318 2314 52-53 -0.6244940778839663 2314 54-55 -0.6330501097399193 2314 56-57 -0.4807415397569379 2314 58-59 -0.48187678490692676 2314 60-61 -0.5079069533502967 2314 62-63 -0.4041194008854987 2314 64-65 -0.2345829138701916 2314 66-67 -0.3153479030496733 2314 68-69 -0.028443969561461557 2314 70-71 0.013214466145551995 2314 72-73 0.07519712139350077 2314 74-75 0.17689690753311638 2314 76-77 0.27691406633102034 2315 1 -0.6984641009642161 2315 2 -0.6836547423888035 2315 3 -0.6723809926558317 2315 4 -0.6323894077029664 2315 5 -0.70616445634667 2315 6 -0.5027106862278572 2315 7 -0.7744381753625618 2315 8 -0.5355955158936041 2315 9 -0.5880982829703143 2315 10-11 -0.702827065128325 2315 12-13 -0.7639749885213618 2315 14-15 -0.8264247541092757 2315 16-17 -0.9004289745779843 2315 18-19 -0.732115876153415 2315 20-21 -0.7606808801282625 2315 22-23 -0.8356690687563386 2315 24-25 -0.8233706291593919 2315 26-27 -0.7403092480875202 2315 28-29 -0.8070533018418331 2315 30-31 -0.7276061288854621 2315 32-33 -0.7387286726564284 2315 34-35 -0.6032577657891451 2315 36-37 -0.8570216092972416 2315 38-39 -0.8045415821830204 2315 40-41 -0.7897718778760989 2315 42-43 -0.9262829301484174 2315 44-45 -0.7095930817358962 2315 46-47 -0.6421111164185405 2315 48-49 -0.6610548919168444 2315 50-51 -0.5560092352301886 2315 52-53 -0.5384162220914703 2315 54-55 -0.7058300455982724 2315 56-57 -0.6247392530010742 2315 58-59 -0.4288937764360874 2315 60-61 -0.48536710129279115 2315 62-63 -0.542053823783224 2315 64-65 -0.42041665943313333 2315 66-67 -0.42525646477099244 2315 68-69 -0.44495916581471207 2315 70-71 -0.5125160111076816 2315 72-73 -1.7980194263337417 2315 74-75 -2.5682988599872765 2315 76-77 -2.281973252463711 2316 1 -2.16873436013541 2316 2 -1.9732907692792026 2316 3 -1.4457238534360144 2316 4 -1.2827686575807888 2316 5 -1.9116361676321816 2316 6 -1.6238965248279769 2316 7 -1.8780163065867157 2316 8 -1.901315187196687 2316 9 -1.3065942604986844 2316 10-11 -1.4118218784191185 2316 12-13 -1.4586045063586326 2316 14-15 -1.4576392571831107 2316 16-17 -2.443551348883453 2316 18-19 -2.235365269394677 2316 20-21 -1.750095968811543 2316 22-23 -1.7393289224771706 2316 24-25 -1.8594168208499795 2316 26-27 -1.6441539014239694 2316 28-29 -1.9237431711269082 2316 30-31 -1.8859615355062118 2316 32-33 -2.1341768527908798 2316 34-35 -1.929408348729261 2316 36-37 -1.9944104728617376 2316 38-39 -1.9743847489092374 2316 40-41 -1.9382667876733493 2316 42-43 -2.2099066042111737 2316 44-45 -1.9562516993526344 2316 46-47 -1.658479639705348 2316 48-49 -1.5867077478922376 2316 50-51 -2.052711794088868 2316 52-53 -3.033656399991898 2316 54-55 -2.3016213370190535 2316 56-57 -2.1024185394281574 2316 58-59 -2.194891673827126 2316 60-61 -2.1019713467559598 2316 62-63 -1.9815288696751274 2316 64-65 -2.0939014052515255 2316 66-67 -2.0260484337903613 2316 68-69 -2.075221950868116 2316 70-71 -1.6391175051099083 2316 72-73 -1.6677447319717693 2316 74-75 -1.8584067418962178 2316 76-77 -1.911990870027065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21169.0 3 2267.0 4 3654.0 5 4840.0 6 5463.0 7 5892.0 8 7672.0 9 8804.0 10 13777.0 11 17205.0 12 11250.0 13 7148.0 14 6271.0 15 5919.0 16 5641.0 17 5925.0 18 6076.0 19 6355.0 20 6689.0 21 7159.0 22 7784.0 23 9073.0 24 10285.0 25 12059.0 26 14348.0 27 17327.0 28 21022.0 29 26004.0 30 32691.0 31 40941.0 32 54414.0 33 75415.0 34 111085.0 35 159413.0 36 234796.0 37 319336.0 38 287707.0 39 82866.0 40 815.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.830028555050042 25.955269918892203 30.68615035334414 21.528551172713613 2 21.345585305269285 26.39534800483907 30.549895129674127 21.70917156021752 3 21.4771122055511 26.487098175084945 30.020783224525637 22.015006394838316 4 21.333035061816773 26.53715717417565 30.427214293171073 21.702593470836504 5 21.535059515216734 26.570141101827872 30.226834282005612 21.667965100949786 6 21.584056005125213 26.256100805407723 30.297813552467222 21.862029636999843 7 21.517733838807146 26.137390645552895 30.34405824680922 22.000817268830737 8 21.452749056842396 26.4406866616874 29.88944594114124 22.217118340328966 9 21.322670109187484 26.33158562564614 30.33530574345385 22.01043852171252 10-11 22.002337386672842 26.508040956168927 29.886065822670027 21.603555834488205 12-13 22.117769947751327 26.08514353396939 29.794599508861875 22.002487009417408 14-15 21.648694015160732 26.46736887062793 29.78461450362555 22.09932261058579 16-17 21.92900228904613 26.368916692141937 29.881223111164136 21.8208579076478 18-19 21.86522108348941 26.47185416981725 29.436278040910267 22.22664670578308 20-21 21.77100659353247 26.671888833472142 29.48929356372913 22.067811009266265 22-23 22.08350584223519 26.884542923993077 28.78015226918309 22.251798964588644 24-25 22.062333596417286 27.115812419352242 28.351203355543348 22.470650628687128 26-27 22.13790963923707 27.375862830142527 28.147950386893932 22.338277143726472 28-29 22.405418399509436 27.226658220176613 28.138294572533635 22.229628807780315 30-31 21.99837452932185 27.292666741229542 28.176117511091224 22.53284121835738 32-33 22.064672877572615 27.62749596458137 27.953162897293378 22.354668260552636 34-35 22.140856227958405 27.12264432323059 28.13342591399543 22.60307353481558 36-37 21.963481637173224 27.306273392907794 28.44854969564054 22.281695274278444 38-39 21.99324542986787 27.249139721412014 28.51892493586499 22.23868991285513 40-41 21.780102213532928 27.303629669066165 28.342079793365816 22.574188324035095 42-43 21.82624807318784 27.50837536925595 28.118989897061702 22.546386660494505 44-45 22.113929143776136 27.213679108953798 28.310008581059094 22.362383166210968 46-47 21.906104804473006 27.381236472281152 28.140280207232127 22.572378516013718 48-49 21.9851659177041 27.423901873723867 28.03101644635285 22.559915762219177 50-51 21.84092669253011 27.779624124841433 27.897823484977764 22.48162569765069 52-53 21.815158832588384 27.568115220827387 28.014161545681336 22.602564400902892 54-55 21.9004756245406 27.607970111782503 27.961287789837435 22.530266473839458 56-57 21.93956445169881 27.840418271456457 27.72879887748235 22.491218399362378 58-59 22.01354284920024 27.76352689152179 27.720217453941963 22.50271280533601 60-61 21.824197412139423 27.707808413962244 27.716548163027042 22.751446010871295 62-63 21.931160632945605 27.64725149045767 27.6441789969114 22.77740887968533 64-65 21.88043835570672 27.66376143751271 27.542115282849682 22.913684923930884 66-67 21.73377904552123 27.760439443738782 27.707937204935927 22.797844305804063 68-69 21.894666683374115 27.415405104961575 27.84207162842152 22.847856583242784 70-71 21.689301182027453 27.237952584631405 27.987121972761003 23.085624260580136 72-73 21.86275914352059 27.04473415590695 27.925956435832695 23.166550264739765 74-75 21.664291866962664 27.173628202472617 27.739550831557953 23.422529099006763 76-77 21.521012850464153 27.03665664071979 28.156195835187642 23.286134673628418 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11053.0 1 6662.0 2 2163.0 3 2099.0 4 2143.0 5 2209.0 6 2344.0 7 2548.0 8 2721.5 9 2760.0 10 2833.5 11 2968.5 12 3194.0 13 3358.0 14 3542.0 15 3975.5 16 4545.5 17 4866.0 18 5201.5 19 5907.5 20 6856.0 21 8530.0 22 9626.0 23 10870.0 24 13300.0 25 16293.0 26 18100.0 27 19477.0 28 23775.5 29 27608.5 30 28520.0 31 31669.0 32 37597.0 33 42844.0 34 47601.5 35 49891.0 36 52762.5 37 57359.5 38 59177.5 39 59270.0 40 61054.0 41 63936.0 42 66881.0 43 68728.0 44 68464.0 45 69238.0 46 70801.0 47 71748.5 48 72171.0 49 72646.5 50 72996.0 51 70899.5 52 68929.0 53 68376.5 54 64875.0 55 58378.0 56 51954.0 57 49078.0 58 47831.5 59 41890.0 60 34562.5 61 31930.0 62 28123.5 63 20601.0 64 14735.5 65 12586.0 66 10866.5 67 8304.0 68 6416.0 69 4523.0 70 3675.0 71 3187.0 72 2379.5 73 1894.5 74 1729.0 75 1534.0 76 1055.0 77 619.0 78 467.0 79 385.5 80 271.0 81 194.0 82 135.0 83 120.0 84 108.0 85 79.5 86 65.5 87 68.0 88 65.0 89 50.0 90 38.0 91 38.0 92 33.5 93 25.5 94 19.0 95 20.0 96 24.0 97 27.0 98 27.5 99 46.5 100 68.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008708323069242501 2 0.011988855732313307 3 0.012227439925991183 4 0.01383788323331685 5 0.010676642667084985 6 0.008290800730306217 7 0.014434343717511542 8 0.01628337121851509 9 0.0158658488795788 10-11 0.019444611784746955 12-13 0.014702750935399153 14-15 0.007217171858755771 16-17 0.005636551575639838 18-19 0.016611424484822168 20-21 0.02200939186678413 22-23 0.018788505252132794 24-25 0.017058769847968187 26-27 0.023083020738334577 28-29 0.007217171858755771 30-31 0.007127702786126568 32-33 0.009006553311339847 34-35 0.010795934763923922 36-37 0.009990713110261088 38-39 0.012555493192298263 40-41 0.00981177496500268 42-43 0.012704608313346936 44-45 0.01136257222390888 46-47 0.02200939186678413 48-49 0.02084629392260448 50-51 0.03429647784119478 52-53 0.036384089535876206 54-55 0.02654249154666379 56-57 0.019384965736327486 58-59 0.014792220008028358 60-61 0.018341159888986772 62-63 0.02350054307727086 64-65 0.022904082593076167 66-67 0.0261249692077275 68-69 0.03924709986001072 70-71 0.04977462740604704 72-73 0.07915030625263561 74-75 0.07920995230105507 76-77 0.083713228956725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1676557.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.61207766800085 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14605245745206 43.67398313500943 2 12.654155886889079 14.327561117831092 3 4.431049240965797 7.525527113408757 4 1.9063050385281968 4.3167955560023845 5 1.0567874091345064 2.991346544224403 6 0.6340119029044277 2.153563865379748 7 0.416365245421827 1.6499911128453695 8 0.2928080277013579 1.3261176644834731 9 0.2214227156061232 1.1281679976018157 >10 1.112592089178923 11.57359573483545 >50 0.08916151184431875 3.4943512551801876 >100 0.03569792132031757 3.473190743633102 >500 0.002745717040616632 1.043844396694684 >1k 7.39231510935247E-4 0.6882437969501263 >5k 0.0 0.0 >10k+ 1.0560450156217816E-4 0.6337199659200259 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10600 0.6322481132463734 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3768 0.22474631044455987 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.9823024209734594E-4 0.0 3 0.0 0.0 0.0 5.368144357752227E-4 0.0 4 0.0 0.0 0.0 0.0011929209683893837 0.0 5 5.964604841946918E-5 0.0 0.0 0.0019086735494230139 0.0 6 5.964604841946918E-5 0.0 0.0 0.004115577340943374 0.0 7 5.964604841946918E-5 0.0 0.0 0.007455756052433649 0.0 8 5.964604841946918E-5 0.0 0.0 0.011392395248118614 0.0 9 5.964604841946918E-5 0.0 0.0 0.015686910734320395 0.0 10 5.964604841946918E-5 0.0 0.0 0.0205182406562974 0.0 11 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.026303907352985913 0.0 12 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.03316320292122487 0.0 13 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.04461524421776295 0.0 14 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.05863206559633821 0.0 15 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.07366286979804444 0.0 16 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.08917084238710643 0.0 17 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.1050963373151047 0.0 18 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.12149900063045874 0.0 19 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.1383788323331685 0.0 20 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.15788309016633492 0.0 21 1.1929209683893837E-4 5.964604841946918E-5 0.0 0.1780434545321155 0.0 22 2.3858419367787673E-4 5.964604841946918E-5 0.0 0.20273691857777576 0.0 23 2.9823024209734594E-4 5.964604841946918E-5 0.0 0.22832507334972804 0.0 24 3.578762905168151E-4 5.964604841946918E-5 0.0 0.25623942401003963 0.0 25 3.578762905168151E-4 5.964604841946918E-5 0.0 0.283616960234576 0.0 26 3.578762905168151E-4 5.964604841946918E-5 0.0 0.31451361331586103 0.0 27 3.578762905168151E-4 5.964604841946918E-5 0.0 0.3447541598645319 0.0 28 3.578762905168151E-4 5.964604841946918E-5 0.0 0.3742193077837497 0.0 29 3.578762905168151E-4 5.964604841946918E-5 0.0 0.4020143663472223 0.0 30 4.175223389362843E-4 5.964604841946918E-5 0.0 0.4361319060431587 0.0 31 4.175223389362843E-4 5.964604841946918E-5 0.0 0.47299316396639063 0.0 32 5.368144357752227E-4 5.964604841946918E-5 0.0 0.5115245112453677 0.0 33 5.368144357752227E-4 5.964604841946918E-5 0.0 0.5567362159473254 5.964604841946918E-5 34 5.368144357752227E-4 5.964604841946918E-5 0.0 0.6021865048429609 5.964604841946918E-5 35 5.368144357752227E-4 5.964604841946918E-5 0.0 0.6527663539026708 5.964604841946918E-5 36 5.368144357752227E-4 5.964604841946918E-5 0.0 0.7082371789327772 5.964604841946918E-5 37 5.368144357752227E-4 5.964604841946918E-5 0.0 0.7631711895271083 5.964604841946918E-5 38 5.368144357752227E-4 5.964604841946918E-5 0.0 0.8173894475404058 5.964604841946918E-5 39 5.368144357752227E-4 5.964604841946918E-5 0.0 0.871070891117928 5.964604841946918E-5 40 5.368144357752227E-4 5.964604841946918E-5 0.0 0.9305976474405583 5.964604841946918E-5 41 5.368144357752227E-4 5.964604841946918E-5 0.0 0.9942996271525514 5.964604841946918E-5 42 5.368144357752227E-4 5.964604841946918E-5 0.0 1.0690361258221461 5.964604841946918E-5 43 5.368144357752227E-4 5.964604841946918E-5 0.0 1.1474706794937481 5.964604841946918E-5 44 5.368144357752227E-4 5.964604841946918E-5 0.0 1.232764528733589 5.964604841946918E-5 45 5.368144357752227E-4 5.964604841946918E-5 0.0 1.31692510305346 5.964604841946918E-5 46 5.368144357752227E-4 5.964604841946918E-5 0.0 1.4118219660888356 5.964604841946918E-5 47 5.964604841946919E-4 5.964604841946918E-5 0.0 1.510774760416735 5.964604841946918E-5 48 5.964604841946919E-4 5.964604841946918E-5 0.0 1.6109204757130238 5.964604841946918E-5 49 5.964604841946919E-4 5.964604841946918E-5 0.0 1.710111854234601 5.964604841946918E-5 50 5.964604841946919E-4 5.964604841946918E-5 0.0 1.814969607356028 5.964604841946918E-5 51 5.964604841946919E-4 5.964604841946918E-5 0.0 1.9267463020941131 5.964604841946918E-5 52 5.964604841946919E-4 5.964604841946918E-5 0.0 2.0425192820763027 5.964604841946918E-5 53 5.964604841946919E-4 5.964604841946918E-5 0.0 2.1715336848076148 5.964604841946918E-5 54 5.964604841946919E-4 5.964604841946918E-5 0.0 2.3124772972228205 5.964604841946918E-5 55 5.964604841946919E-4 5.964604841946918E-5 0.0 2.4583715316568417 5.964604841946918E-5 56 7.157525810336302E-4 5.964604841946918E-5 0.0 2.623412147633513 5.964604841946918E-5 57 7.157525810336302E-4 5.964604841946918E-5 0.0 2.78332320344611 5.964604841946918E-5 58 7.753986294530994E-4 5.964604841946918E-5 0.0 2.9521811665216275 5.964604841946918E-5 59 7.753986294530994E-4 5.964604841946918E-5 0.0 3.120144438870853 5.964604841946918E-5 60 7.753986294530994E-4 5.964604841946918E-5 0.0 3.2856025771864603 5.964604841946918E-5 61 8.350446778725686E-4 5.964604841946918E-5 0.0 3.468119485350036 5.964604841946918E-5 62 8.350446778725686E-4 5.964604841946918E-5 0.0 3.6502188711746753 5.964604841946918E-5 63 8.350446778725686E-4 5.964604841946918E-5 0.0 3.847229769104182 5.964604841946918E-5 64 8.350446778725686E-4 5.964604841946918E-5 0.0 4.049310581149344 5.964604841946918E-5 65 8.350446778725686E-4 5.964604841946918E-5 0.0 4.270895651027671 5.964604841946918E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCGCGA 35 1.92164E-5 50.706337 25 CGCGTCA 50 1.5762227E-4 35.49126 2 TCGCGAT 55 2.7579875E-4 32.266705 26 CGCGGAA 165 5.8591468E-8 21.514347 59 CGGTTAC 185 8.4928615E-9 21.103542 4 CGCTATT 105 4.9726595E-4 20.283745 17 CGGTATA 125 7.027263E-5 19.877478 10 GCACGAC 205 2.7614078E-8 19.046364 24 GGGCGTA 230 5.113179E-9 18.518837 34 CGTGCGA 160 2.3058157E-5 17.753044 52 ACGCACG 160 2.3064073E-5 17.752514 49 GCCGTAA 140 1.6764448E-4 17.747217 39 ACGTTAT 100 0.0089446865 17.74563 2 CGTAGGG 265 1.5570549E-9 17.41756 49 CGGGTTA 265 1.5643309E-9 17.411325 28 TCCGCGA 290 2.7830538E-10 17.14138 51 TACGCAC 145 2.1872943E-4 17.140358 48 TACCGCG 125 0.0016054099 17.036821 5 AATTCGC 190 5.3508793E-6 16.816164 55 CGGCGTA 275 2.5484042E-9 16.783695 46 >>END_MODULE