FastQCFastQC Report
Thu 26 May 2016
SRR936007_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936007_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676557
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114770.6845576977102479No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85220.5083036246307164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64700.38590993327396567No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17900.10676642667084985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13350.051.05254100-101
GGTATCA82650.045.650851
CGGTATC9300.044.16307100-101
CCGTACA4900.041.302467100-101
CGTACAT12400.040.322803100-101
TCGTATC4800.039.68276100-101
AGGTATC18950.038.949833100-101
AGTATCA25250.038.89696100-101
CCGTATC5400.036.927013100-101
ACGTATC3900.036.63024100-101
CGAGTAC10400.036.344067100-101
TACGCAG8100.035.640995100-101
TCGGTAT3200.035.342457100-101
GGGTATC11050.034.74486100-101
GCGTATC3800.034.461346100-101
ACCGTAT2050.033.3915998-99
GCGTACA3600.032.24224100-101
TCGAGTA3250.032.051456100-101
GTATCAA168500.031.786671
TGTATCA23100.031.56583100-101
Produced by FastQC (version 0.11.5)