FastQCFastQC Report
Thu 26 May 2016
SRR936006_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936006_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691678
Sequences flagged as poor quality0
Sequence length77
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106170.6276017067077777No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39360.23266839197530498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGT500.00673605728.39374526
CGGTTAC1951.8189894E-1225.4830724
CTTACCG800.002456947222.18720423
TCGCGCT1355.341126E-621.03240437
TCGTTAC1407.3107713E-620.28304510
TAGCGCG1054.9748365E-420.2824465
TATACCG1106.788123E-419.3679646
CGCGTAA1159.1638457E-418.52258752
CAATTCG1551.7593286E-518.3201754
CGCGCTT1803.2022253E-617.7471438
CTCGCGC1602.3136154E-517.7460936
AATTCGC1653.0120687E-517.20934955
GCGTAAC1250.0016032417.04027753
TCGCGGT1956.8641E-616.3814933
CTTCGCG2008.729745E-615.97195231
TTGCGTA2905.176844E-915.91028836
CGCGCGT1350.002684308415.7752368
ATTCGCG1806.346341E-515.77523656
ATCGTAC1350.002684837715.77476933
CGACCGC1350.002685367115.774302537
Produced by FastQC (version 0.11.5)