##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936006_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1691678 Sequences flagged as poor quality 0 Sequence length 77 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 38.072062177317434 40.0 38.0 41.0 34.0 41.0 2 37.949463786843594 40.0 38.0 41.0 33.0 41.0 3 37.828862230282596 40.0 38.0 41.0 33.0 41.0 4 37.73683998964342 40.0 38.0 41.0 33.0 41.0 5 37.653178087082765 40.0 37.0 41.0 33.0 41.0 6 37.52959724013672 40.0 37.0 41.0 33.0 41.0 7 37.351023658166625 40.0 37.0 41.0 32.0 41.0 8 37.20845751969346 40.0 36.0 41.0 32.0 41.0 9 37.32597220038329 40.0 37.0 41.0 33.0 41.0 10-11 37.52047966575199 40.0 37.0 41.0 33.0 41.0 12-13 37.43351187400912 40.0 37.0 41.0 33.0 41.0 14-15 37.27028045526394 40.0 37.0 41.0 32.5 41.0 16-17 37.08304624166065 40.0 37.0 41.0 32.0 41.0 18-19 36.86580365767008 40.0 36.5 41.0 31.0 41.0 20-21 36.5643053819935 40.0 36.0 41.0 30.5 41.0 22-23 36.15299690603058 40.0 36.0 41.0 29.5 41.0 24-25 35.81605660178829 40.0 35.0 41.0 28.5 41.0 26-27 34.821874198281236 38.0 34.0 40.0 26.0 40.5 28-29 34.824759794712705 38.5 35.0 40.0 26.0 40.5 30-31 35.19968486910629 39.0 35.0 40.5 27.0 41.0 32-33 35.06091673474503 39.0 35.0 41.0 26.0 41.0 34-35 34.80870177421471 38.5 35.0 40.5 26.0 41.0 36-37 34.506052570288205 38.0 34.5 40.0 25.5 41.0 38-39 34.20352898128367 37.5 34.0 40.0 24.5 41.0 40-41 33.85977709705984 37.0 34.0 40.0 23.0 41.0 42-43 33.496356871697806 36.5 34.0 39.5 22.0 41.0 44-45 33.134254864105344 36.0 33.5 39.0 21.5 41.0 46-47 32.72027803163486 35.5 33.0 39.0 20.0 40.5 48-49 32.321480506337494 35.0 33.0 38.0 18.5 40.0 50-51 31.895600699423888 35.0 32.5 37.0 16.0 39.5 52-53 31.446368930730316 35.0 32.0 37.0 10.0 39.0 54-55 31.013587692220387 35.0 32.0 36.0 7.0 39.0 56-57 30.613491456411918 35.0 31.0 36.0 3.5 37.5 58-59 30.21131681088245 35.0 31.0 35.0 2.0 37.0 60-61 29.880121098696087 34.0 31.0 35.0 2.0 36.5 62-63 29.547902437697957 34.0 30.5 35.0 2.0 36.0 64-65 29.201447024788408 34.0 30.0 35.0 2.0 36.0 66-67 28.88544214679153 34.0 30.0 35.0 2.0 35.5 68-69 28.56741944980073 34.0 29.0 35.0 2.0 35.0 70-71 28.164850225633955 34.0 29.0 35.0 2.0 35.0 72-73 27.744505455529954 34.0 29.0 35.0 2.0 35.0 74-75 27.255858679961555 34.0 27.5 35.0 2.0 35.0 76-77 25.557665524999436 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6381883393110925 1101 2 0.6503039600732663 1101 3 0.758854195963643 1101 4 0.6453341836944517 1101 5 0.5591184281818471 1101 6 0.5858310543436644 1101 7 0.646361229304155 1101 8 0.6746772642733845 1101 9 0.6630735198713111 1101 10-11 0.6116610702899905 1101 12-13 0.6452484454730154 1101 14-15 0.7143191748210995 1101 16-17 0.6935719493609369 1101 18-19 0.6882207941938177 1101 20-21 0.7798799851220295 1101 22-23 0.6916622178298582 1101 24-25 0.7053310933703756 1101 26-27 0.6781367924019577 1101 28-29 0.6486337832071811 1101 30-31 0.6491468027160749 1101 32-33 0.7370784889237925 1101 34-35 0.6574086621143138 1101 36-37 0.6278407503010115 1101 38-39 0.687368813501898 1101 40-41 0.46980159823184664 1101 42-43 0.4975402897793586 1101 44-45 0.39160148873889256 1101 46-47 0.4299639923909382 1101 48-49 0.4415871046000106 1101 50-51 0.3119351030064337 1101 52-53 0.3744566977721 1101 54-55 0.38593813734039983 1101 56-57 0.47723080731862666 1101 58-59 0.5313564901981422 1101 60-61 0.36059504855874636 1101 62-63 0.4272049065666934 1101 64-65 0.35355487980439193 1101 66-67 0.5067368637614429 1101 68-69 0.3962079874512874 1101 70-71 0.16366214482153296 1101 72-73 0.07974148249251911 1101 74-75 0.16478634236829137 1101 76-77 0.2588567112900293 1102 1 0.4530974675994699 1102 2 0.6088263035408374 1102 3 0.5443945535766019 1102 4 0.569397498863232 1102 5 0.531754789148259 1102 6 0.603008838232661 1102 7 0.4147705271342872 1102 8 0.5884492613150769 1102 9 0.608258133489656 1102 10-11 0.7524134351972478 1102 12-13 0.8457432537573268 1102 14-15 0.8070778728472092 1102 16-17 0.8100548122937639 1102 18-19 0.7751750232594148 1102 20-21 0.8039058542571524 1102 22-23 0.7042086415667725 1102 24-25 0.8747199954606728 1102 26-27 0.6779866077749901 1102 28-29 0.8376201103987384 1102 30-31 0.890650024009453 1102 32-33 0.8619229643989783 1102 34-35 0.7656367104617559 1102 36-37 0.7627762977339856 1102 38-39 0.878889923453066 1102 40-41 0.879140812572679 1102 42-43 1.0128831512704224 1102 44-45 0.951274179700313 1102 46-47 0.9215940362581989 1102 48-49 0.8008213899481262 1102 50-51 0.942331403525209 1102 52-53 1.033545604401315 1102 54-55 0.9888446437389327 1102 56-57 0.9957555804397167 1102 58-59 1.020592257367916 1102 60-61 0.9403657800446545 1102 62-63 0.8591512459332229 1102 64-65 0.8312628087517133 1102 66-67 0.8861666427697692 1102 68-69 0.6607742275491155 1102 70-71 0.5694323049383705 1102 72-73 0.48586941576515486 1102 74-75 0.46129718636584727 1102 76-77 0.288472452240903 1103 1 0.25219759861996494 1103 2 0.09855926622524436 1103 3 0.18817426905216905 1103 4 0.3169019968793165 1103 5 0.09118441147436585 1103 6 0.3031207238913609 1103 7 0.30183643868349463 1103 8 0.2533264661402441 1103 9 0.31920379320310843 1103 10-11 0.27951082621503787 1103 12-13 0.32694094854069533 1103 14-15 0.4374186855995177 1103 16-17 0.3664760126328801 1103 18-19 0.39912526470389054 1103 20-21 0.47247548514077664 1103 22-23 0.4662599362246951 1103 24-25 0.486062835974451 1103 26-27 0.39200646548619744 1103 28-29 0.5857282195842615 1103 30-31 0.7721615168724227 1103 32-33 0.6669216754549296 1103 34-35 0.7348502154062473 1103 36-37 0.6949037596835623 1103 38-39 0.7708358834771971 1103 40-41 0.7995794123276525 1103 42-43 0.8219516699415053 1103 44-45 0.6641884689074615 1103 46-47 0.5991995635691936 1103 48-49 0.5029032339088317 1103 50-51 0.4298017378799557 1103 52-53 0.7469811186634168 1103 54-55 0.7843549991419998 1103 56-57 0.6801988230508798 1103 58-59 0.5604860722968112 1103 60-61 0.5438321093711878 1103 62-63 0.42917545682041336 1103 64-65 0.4016846655221258 1103 66-67 0.32175631474015276 1103 68-69 0.36414468335693684 1103 70-71 0.3911789835820265 1103 72-73 0.2544694099183964 1103 74-75 0.29022707046718565 1103 76-77 0.551985103824709 1104 1 0.04814028781520108 1104 2 0.05596834907776582 1104 3 0.15223993944626102 1104 4 0.03776115631732324 1104 5 0.22749201261613905 1104 6 0.058454591603506856 1104 7 -0.004395688064811054 1104 8 0.06663382125613282 1104 9 0.07610243847346254 1104 10-11 0.13218992909353489 1104 12-13 0.19287238139018115 1104 14-15 0.23500816098237465 1104 16-17 0.15486464888228113 1104 18-19 0.2102213629449281 1104 20-21 0.3605399888959795 1104 22-23 0.315035031526655 1104 24-25 0.2403398505428811 1104 26-27 0.01614438283737485 1104 28-29 0.09880892665736951 1104 30-31 0.18310675891692085 1104 32-33 0.25624692961554274 1104 34-35 0.2520416050292482 1104 36-37 0.2307451682214534 1104 38-39 0.3442580771286714 1104 40-41 0.25479972454243693 1104 42-43 0.39447408342901724 1104 44-45 0.24803435617469916 1104 46-47 0.3366472830679328 1104 48-49 0.24487961542354952 1104 50-51 0.15888457717743165 1104 52-53 0.21993711971100538 1104 54-55 0.1516365982573653 1104 56-57 0.1798291228585569 1104 58-59 0.21248146129548928 1104 60-61 0.24318847390219034 1104 62-63 0.11771792538597836 1104 64-65 0.1875280581397618 1104 66-67 0.08266048633395329 1104 68-69 -0.05637936501227969 1104 70-71 -0.011144439934344774 1104 72-73 0.006132730103896478 1104 74-75 -0.22003657404921384 1104 76-77 -0.1362346211024743 1105 1 -0.07513266873266389 1105 2 0.12733278520348534 1105 3 0.11788921271310926 1105 4 0.1272297151640558 1105 5 0.058824560292009664 1105 6 -0.027367592326164925 1105 7 0.02814175445510614 1105 8 0.13212860389640468 1105 9 0.11643043395758212 1105 10-11 0.1266015277744259 1105 12-13 0.374398702565621 1105 14-15 0.28640072232472846 1105 16-17 0.31300049499905214 1105 18-19 0.14424759334063708 1105 20-21 0.04942821756851856 1105 22-23 0.06786075751706733 1105 24-25 0.10176489452979354 1105 26-27 -0.10366111742506234 1105 28-29 0.06203603117952383 1105 30-31 0.3034102273635355 1105 32-33 0.18591655735254875 1105 34-35 0.0129501947741133 1105 36-37 -0.03882463480299947 1105 38-39 -0.02994281350319028 1105 40-41 0.0734346612111807 1105 42-43 0.07219343205956363 1105 44-45 -0.03683315490868466 1105 46-47 0.022058812426109853 1105 48-49 -0.026698565746414715 1105 50-51 -0.08683325955766463 1105 52-53 -0.12101626571339708 1105 54-55 -0.15299283880916548 1105 56-57 -0.047052576274253965 1105 58-59 -0.141916074777086 1105 60-61 -0.010728259611379798 1105 62-63 -0.038750214223369994 1105 64-65 0.059208242736254135 1105 66-67 0.06640909575325793 1105 68-69 0.0040703743673127235 1105 70-71 -0.2097419753466987 1105 72-73 -0.626172316259801 1105 74-75 -0.7452715285438032 1105 76-77 -0.8104025009166911 1106 1 -0.08962616300704696 1106 2 -0.14468591040233747 1106 3 -0.331811518832005 1106 4 -0.32620607622628484 1106 5 -0.2149056428924041 1106 6 -0.18650158370601844 1106 7 -0.5226012131169568 1106 8 -0.19214810054617715 1106 9 -0.31584385400010007 1106 10-11 -0.41061809313527675 1106 12-13 -0.15316022463692036 1106 14-15 -0.22054396420065814 1106 16-17 -0.2020069253694814 1106 18-19 -0.13059287296649558 1106 20-21 -0.22871393198617795 1106 22-23 -0.1273093186834373 1106 24-25 -0.2116371202229459 1106 26-27 -0.2426550271385608 1106 28-29 -0.2192096430925261 1106 30-31 -0.06347732993913979 1106 32-33 -0.1664561837501921 1106 34-35 -0.17973198500357057 1106 36-37 -0.20379575713162268 1106 38-39 -0.11080824676979262 1106 40-41 -0.17869947739148273 1106 42-43 -0.10492526506925515 1106 44-45 -0.23923627129424574 1106 46-47 -0.21958358503827924 1106 48-49 -0.27818610849602976 1106 50-51 -0.168066749872267 1106 52-53 -0.29083677232911853 1106 54-55 -0.24546366076939208 1106 56-57 -0.2625190949284004 1106 58-59 -0.391977417618417 1106 60-61 -0.6398232115608984 1106 62-63 -0.6083700239844134 1106 64-65 -0.636835355524827 1106 66-67 -0.6362011479089702 1106 68-69 -0.460059885675566 1106 70-71 -0.45236416605609264 1106 72-73 -0.5440257060229037 1106 74-75 -0.633512994273886 1106 76-77 -0.545581478174995 1107 1 -0.09226855877921736 1107 2 0.05956869220206329 1107 3 0.027916987955329375 1107 4 -0.10672747445087083 1107 5 -0.02404867767531016 1107 6 -0.2929011899372327 1107 7 -0.1514007364494816 1107 8 -0.19765740937833698 1107 9 -0.20666800515204642 1107 10-11 -0.18885545972019457 1107 12-13 -0.10231785304353735 1107 14-15 -0.22445574617710662 1107 16-17 -0.14617120294192176 1107 18-19 -0.15067853494708316 1107 20-21 -0.07381643276335126 1107 22-23 -0.07286387638437475 1107 24-25 -0.08723079574365045 1107 26-27 -0.27247265001859233 1107 28-29 -0.1601831845936772 1107 30-31 0.037205720732679026 1107 32-33 0.1544632145434761 1107 34-35 0.04511128465896519 1107 36-37 0.10427866171401234 1107 38-39 0.012514350031700872 1107 40-41 -0.016503007494073074 1107 42-43 -0.02870812231080322 1107 44-45 -0.11481051743427173 1107 46-47 -0.08183575249363173 1107 48-49 -0.14546185026660652 1107 50-51 -0.15636378624406788 1107 52-53 -0.09391568152051022 1107 54-55 -0.09125698397066273 1107 56-57 -0.05672093597015859 1107 58-59 -0.1326772207340241 1107 60-61 -0.07633015432626067 1107 62-63 -0.13655557888752412 1107 64-65 -0.1949551095920441 1107 66-67 -0.24828276857837395 1107 68-69 -0.3967416928670282 1107 70-71 -0.34025448072570796 1107 72-73 -0.24465996736102724 1107 74-75 -0.25874398802194065 1107 76-77 0.015836952967280382 1108 1 0.221938618399264 1108 2 0.3258135326342355 1108 3 0.3417091079806909 1108 4 0.2959559464349084 1108 5 0.24134472266131723 1108 6 0.3847423167100459 1108 7 0.2715020790639784 1108 8 0.3100657093050927 1108 9 0.26226700402759207 1108 10-11 0.32061128096075464 1108 12-13 0.43363090267623505 1108 14-15 0.43700084979403897 1108 16-17 0.44768009455729185 1108 18-19 0.3593439494373527 1108 20-21 0.3451231280399796 1108 22-23 0.3656191788436871 1108 24-25 0.4678557869609392 1108 26-27 0.3485349712455772 1108 28-29 0.33581870735395114 1108 30-31 0.4214286471657118 1108 32-33 0.4861911191159791 1108 34-35 0.48811524807282325 1108 36-37 0.4409468286128231 1108 38-39 0.5861056702444714 1108 40-41 0.38769581761408034 1108 42-43 0.41314011064066136 1108 44-45 0.5313309788594935 1108 46-47 0.5268371277229136 1108 48-49 0.4672325327114635 1108 50-51 0.30575795154675944 1108 52-53 0.47594458044930477 1108 54-55 0.4757013791763356 1108 56-57 0.5098456055852125 1108 58-59 0.5627281220446179 1108 60-61 0.6823050397193242 1108 62-63 0.6043214394233694 1108 64-65 0.745551349257461 1108 66-67 0.6695833411138459 1108 68-69 0.5684587568206574 1108 70-71 0.5518610566714521 1108 72-73 0.6063540898353743 1108 74-75 0.6867655964880228 1108 76-77 0.8141475048405127 1109 1 -0.060609741406111084 1109 2 -0.1615492421511604 1109 3 -0.20604839306887612 1109 4 -0.19322745906009686 1109 5 -0.14060299170861867 1109 6 -0.34459494436207905 1109 7 -0.27290446614649255 1109 8 -0.13138731159563832 1109 9 -0.1736111926834667 1109 10-11 -0.28511960640451406 1109 12-13 -0.1736534385597963 1109 14-15 -0.17624671897647914 1109 16-17 -0.15090130010899827 1109 18-19 -0.25881281429985137 1109 20-21 -0.17597203859542532 1109 22-23 -0.24426961816826775 1109 24-25 -0.445951848267228 1109 26-27 -0.45161866739990586 1109 28-29 -0.3424445506785787 1109 30-31 -0.24466985000843522 1109 32-33 -0.3431698130265346 1109 34-35 -0.17017418850466015 1109 36-37 -0.26288166395471535 1109 38-39 -0.31613185203887184 1109 40-41 -0.267513668168597 1109 42-43 -0.3160070162273456 1109 44-45 -0.2631018852634597 1109 46-47 -0.23949176361603008 1109 48-49 -0.2641965236231272 1109 50-51 -0.20891236978004457 1109 52-53 -0.1810640023873198 1109 54-55 -0.18957772252249327 1109 56-57 -0.20859514719310823 1109 58-59 -0.12183955233333421 1109 60-61 -0.19278763475432825 1109 62-63 -0.23233876395642739 1109 64-65 -0.23612542387159863 1109 66-67 -0.1499703666945429 1109 68-69 -0.17786696348809627 1109 70-71 -0.3081089301978004 1109 72-73 -0.38729715043938384 1109 74-75 -0.3596939834047106 1109 76-77 -0.24624993306918697 1110 1 -0.3722888685024941 1110 2 -0.3229118069790786 1110 3 -0.40171081645407014 1110 4 -0.4121044487998873 1110 5 -0.33426725609423613 1110 6 -0.4662525182827366 1110 7 -0.4216662811034979 1110 8 -0.5286561201091402 1110 9 -0.4777916107252764 1110 10-11 -0.47371550681087626 1110 12-13 -0.49701000891071345 1110 14-15 -0.5748457625543111 1110 16-17 -0.6152038528132451 1110 18-19 -0.7045229465375584 1110 20-21 -0.8037223136229272 1110 22-23 -0.6563365901715983 1110 24-25 -0.6614611542998716 1110 26-27 -0.6873958165433933 1110 28-29 -0.49139158823948037 1110 30-31 -0.523104326109106 1110 32-33 -0.45194038396117975 1110 34-35 -0.3580544561232628 1110 36-37 -0.3721321298176221 1110 38-39 -0.4828658723796835 1110 40-41 -0.40303487335034305 1110 42-43 -0.44086950247596235 1110 44-45 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-0.43757380199881624 2313 76-77 -0.39162408272905225 2314 1 -0.3525439780332178 2314 2 -0.34202961661836184 2314 3 -0.3161396563726626 2314 4 -0.27585754569113163 2314 5 -0.24303605719180865 2314 6 -0.21753703717944006 2314 7 -0.3220999818047048 2314 8 -0.285895188332276 2314 9 -0.2512242258154487 2314 10-11 -0.3345271233926326 2314 12-13 -0.21951903905589631 2314 14-15 -0.3510562913501829 2314 16-17 -0.3408133989408455 2314 18-19 -0.23659423439122662 2314 20-21 -0.32110749530419014 2314 22-23 -0.14225355089567415 2314 24-25 -0.22917908754294558 2314 26-27 -0.1153173154478111 2314 28-29 -0.2919816966737159 2314 30-31 -0.35034083888724155 2314 32-33 -0.4888362069866403 2314 34-35 -0.4902443637466405 2314 36-37 -1.1781824630222815 2314 38-39 -0.7558422227330084 2314 40-41 -0.6033387175733154 2314 42-43 -0.5567630111019497 2314 44-45 -0.5817851795586577 2314 46-47 -0.4487940457589801 2314 48-49 -0.3018673854879168 2314 50-51 -0.2962083535222533 2314 52-53 -0.2379751523497724 2314 54-55 -0.2676432430744846 2314 56-57 -0.20375227099815163 2314 58-59 -0.16208898543990813 2314 60-61 -0.23896843546560476 2314 62-63 -0.2065086900268014 2314 64-65 -0.11909088353157671 2314 66-67 -0.15745672202745453 2314 68-69 -0.12759509746546982 2314 70-71 -0.0049787819276225775 2314 72-73 -0.017321208387659937 2314 74-75 -0.10388169143685033 2314 76-77 -0.09748990736752816 2315 1 -0.624903944287496 2315 2 -0.6813398436306812 2315 3 -0.4143598468823626 2315 4 -0.4287484811659823 2315 5 -0.5440837107518917 2315 6 -0.5209119598508067 2315 7 -0.8080992366451341 2315 8 -0.8866416865125615 2315 9 -0.6410774982229057 2315 10-11 -0.6947242878979196 2315 12-13 -0.5033072288822709 2315 14-15 -0.5149617561617887 2315 16-17 -0.5596179692103789 2315 18-19 -0.6248803769410713 2315 20-21 -0.6270828272715079 2315 22-23 -0.643044924382842 2315 24-25 -0.7517370565273538 2315 26-27 -0.7777847306524492 2315 28-29 -0.7274941596109485 2315 30-31 -0.755493467518896 2315 32-33 -0.7067686090936149 2315 34-35 -0.8855852997473761 2315 36-37 -0.8596195756022595 2315 38-39 -0.6441071706244301 2315 40-41 -0.6942781445143709 2315 42-43 -0.9267473270547129 2315 44-45 -0.8367879954204227 2315 46-47 -0.759757134225481 2315 48-49 -0.7056995709631728 2315 50-51 -0.7198876633604314 2315 52-53 -0.6262479087995629 2315 54-55 -0.7287642715859199 2315 56-57 -0.7975472399280541 2315 58-59 -0.5924430191021273 2315 60-61 -0.6685957951243005 2315 62-63 -0.580660226416434 2315 64-65 -0.3957095419065091 2315 66-67 -0.6039069035844058 2315 68-69 -0.8069599142687203 2315 70-71 -0.691645397599622 2315 72-73 -0.45781138824904133 2315 74-75 -0.49545475898597857 2315 76-77 -0.5302972625833107 2316 1 -1.3647257952843859 2316 2 -1.0625753871852979 2316 3 -1.0510064384263913 2316 4 -1.9292755400767945 2316 5 -1.3133431929822024 2316 6 -1.3521472329206432 2316 7 -0.9960189227591414 2316 8 -0.8132498924024816 2316 9 -1.1282642478691471 2316 10-11 -1.0615997945738727 2316 12-13 -1.4878652528905008 2316 14-15 -2.599483612491099 2316 16-17 -1.7179879661113944 2316 18-19 -1.4719500221345996 2316 20-21 -1.181316764146544 2316 22-23 -1.616253986704855 2316 24-25 -1.4426780576146996 2316 26-27 -1.3399844442477118 2316 28-29 -1.924398770449045 2316 30-31 -2.034162921982009 2316 32-33 -2.049325022558186 2316 34-35 -1.922117553511562 2316 36-37 -2.0622387569916683 2316 38-39 -2.2157954902416037 2316 40-41 -1.9113475887763443 2316 42-43 -2.143644240668827 2316 44-45 -2.002438476535559 2316 46-47 -1.937192637130103 2316 48-49 -1.7738171051002851 2316 50-51 -1.8414597518043152 2316 52-53 -1.903849721202139 2316 54-55 -1.9398672080459534 2316 56-57 -1.6867184204704628 2316 58-59 -1.776602966798098 2316 60-61 -1.5529687689035079 2316 62-63 -1.7848194322113002 2316 64-65 -1.6728345282856694 2316 66-67 -1.6040519993476074 2316 68-69 -1.6833135939822839 2316 70-71 -1.6401107056083681 2316 72-73 -1.3624678223477424 2316 74-75 -1.5257480881358134 2316 76-77 -1.9303110186663162 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 21504.0 3 2373.0 4 3749.0 5 5094.0 6 5844.0 7 6109.0 8 8037.0 9 9077.0 10 13910.0 11 17462.0 12 11471.0 13 7291.0 14 6477.0 15 6197.0 16 5840.0 17 5978.0 18 6303.0 19 6591.0 20 7094.0 21 7459.0 22 8007.0 23 9293.0 24 10600.0 25 12545.0 26 14927.0 27 17770.0 28 21487.0 29 26427.0 30 32694.0 31 41714.0 32 55159.0 33 74860.0 34 111442.0 35 160034.0 36 236619.0 37 321147.0 38 290730.0 39 81641.0 40 722.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 21.803742787269297 26.01956577898083 30.64889600366984 21.527795430080037 2 21.443920987648482 26.316246860096733 30.64019442718528 21.59963772506951 3 21.532153889356923 26.416648191667036 30.01090763978185 22.040290279194195 4 21.291551596613616 26.528221946704463 30.480246381464394 21.699980075217532 5 21.535479995648657 26.60526701635064 30.21102828845617 21.648224699544528 6 21.6289366441869 26.218616368115583 30.325277706011477 21.827169281686036 7 21.51224423794481 26.130756955764184 30.3121676135339 22.0448311927571 8 21.426861044054185 26.46083096983532 29.929186702448757 22.183121283661738 9 21.332246245496993 26.384366746700454 30.286945072305333 21.99644193549722 10-11 22.03243819799877 26.446476204666386 29.908558964848815 21.61252663248603 12-13 22.12270476217505 26.034276024520373 29.79339312067204 22.049626092632536 14-15 21.741460428488285 26.45846135929057 29.732290149014084 22.06778806320706 16-17 21.983076117273026 26.3682227322009 29.8241460320388 21.82455511848728 18-19 21.91174679598977 26.45810695287069 29.429243369983816 22.200902881155727 20-21 21.755438283160515 26.683214075525118 29.464142403488967 22.097205237825403 22-23 22.160722467741696 26.891881106167876 28.691901798779135 22.25549462731129 24-25 22.095523697636594 27.11042617964926 28.352940202645378 22.441109920068776 26-27 22.237472010168716 27.30175809395715 28.126015208732085 22.334754687142045 28-29 22.454712193160976 27.202420276168056 28.12495104320465 22.217916487466322 30-31 21.994321177997904 27.29632857004353 28.181848815404585 22.527501436553973 32-33 22.086633991883627 27.643889203694787 27.903655798029998 22.365821006391595 34-35 22.11948887606652 27.10530609735334 28.09886916135379 22.676335865226356 36-37 21.986354799452915 27.337370947446082 28.42690009905348 22.249374154047523 38-39 21.972247759985102 27.28295282887794 28.521900561370938 22.222898849766022 40-41 21.848187042814416 27.303612316674975 28.28376973070058 22.564430909810028 42-43 21.827926156938677 27.517123135817435 28.09453567258377 22.560415034660124 44-45 22.109171532037543 27.183474983371518 28.34699578745104 22.3603576971399 46-47 21.939663250772877 27.37197492974784 28.15951453434921 22.52884728513007 48-49 22.01188161660222 27.4195631937705 27.981659376512212 22.586895813115067 50-51 21.864329312458132 27.740639063134697 27.914237753339677 22.480793871067494 52-53 21.90055742573028 27.550088193537363 27.982777573119627 22.56657680761273 54-55 21.949138537339017 27.551136550087346 27.971971532811974 22.527753379761666 56-57 21.957128127268817 27.790627002764555 27.74105303734395 22.511191832622686 58-59 22.027922931550123 27.744825235580706 27.730399363133206 22.496852469735966 60-61 21.85918707310548 27.72199254149404 27.690952385879392 22.727867999521095 62-63 21.944764474292217 27.64726768419898 27.61483602202751 22.79313181948129 64-65 21.90883618740056 27.60642666211009 27.577928362843657 22.90680878764569 66-67 21.68503628347911 27.724054585299363 27.77513850749216 22.81577062372937 68-69 21.88769992449153 27.38275935267304 27.816800013718062 22.912740709117365 70-71 21.661281708708373 27.241144982230526 27.996813382374597 23.100759926686504 72-73 21.91095009242144 27.021426987060998 27.930292051756005 23.137330868761552 74-75 21.703275657522873 27.100551952152514 27.779955035761095 23.41621735456352 76-77 21.530170431371225 26.979372134176515 28.16481707088032 23.32564036357194 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 11140.0 1 6681.5 2 2136.5 3 2070.5 4 2091.0 5 2160.5 6 2347.5 7 2569.0 8 2714.5 9 2756.0 10 2849.5 11 2987.5 12 3291.5 13 3551.0 14 3663.5 15 4003.0 16 4502.5 17 4775.0 18 5109.0 19 5793.5 20 6836.5 21 8521.0 22 9513.0 23 10809.5 24 13250.0 25 16343.0 26 18292.0 27 19731.5 28 24082.0 29 27835.0 30 28677.0 31 31864.5 32 37955.5 33 43208.5 34 48023.5 35 50489.0 36 53207.5 37 57579.0 38 59741.5 39 60251.0 40 61951.0 41 64620.0 42 67577.0 43 69565.0 44 69089.0 45 69611.0 46 71402.0 47 72590.0 48 72985.0 49 73441.0 50 73838.5 51 71586.0 52 69392.0 53 68894.0 54 65370.0 55 58690.0 56 52575.0 57 50114.0 58 48522.0 59 42171.0 60 34828.5 61 32245.0 62 28523.0 63 21043.0 64 14969.5 65 12654.0 66 10952.0 67 8427.0 68 6571.5 69 4593.0 70 3647.0 71 3191.0 72 2419.5 73 1910.5 74 1717.0 75 1496.5 76 1058.5 77 670.5 78 500.0 79 434.0 80 305.0 81 201.5 82 144.0 83 127.0 84 103.5 85 81.0 86 72.0 87 62.0 88 58.0 89 45.0 90 31.5 91 27.0 92 25.0 93 20.5 94 20.5 95 18.5 96 14.0 97 15.0 98 16.0 99 37.5 100 59.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030147581277287995 2 0.008571371147464235 3 0.011999919606449928 4 0.018738790715490772 5 0.014541774498456561 6 0.013655080931477504 7 0.01838411328869915 8 0.01944814556907402 9 0.01826588747976861 10-11 0.02184221819991748 12-13 0.0206895165628447 14-15 0.0074777824148567285 16-17 0.00614774206438814 18-19 0.012059032510915198 20-21 0.010315201829189716 22-23 0.014955564829713457 24-25 0.020630403658379433 26-27 0.01273883091226581 28-29 0.008009798555044164 30-31 0.007684677580485175 32-33 0.009428508262210656 34-35 0.01123145184840141 36-37 0.009901411497932822 38-39 0.016728951963671572 40-41 0.012709274460033173 42-43 0.014955564829713457 44-45 0.017911210052976984 46-47 0.016019597110088327 48-49 0.014275766428362844 50-51 0.02488653277987891 52-53 0.030206694181753264 54-55 0.023497379524945055 56-57 0.015724032587761973 58-59 0.016108266466786234 60-61 0.01894568588111922 62-63 0.025388992467833715 64-65 0.020630403658379433 66-67 0.020305282683820442 68-69 0.028196855429934064 70-71 0.050659759126736884 72-73 0.06224588840192992 74-75 0.07232463861325855 76-77 0.06936899338999503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 1691678.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.60338497395044 #Duplication Level Percentage of deduplicated Percentage of total 1 77.37740967869136 43.79823308330047 2 12.355512964950432 13.987277138114504 3 4.359960459661867 7.403655611083279 4 2.0314563351189707 4.599492199780383 5 1.0847828931746302 3.070119185775968 6 0.6348087745274633 2.1559395269651827 7 0.39898740957516543 1.5808826560759632 8 0.292351606500145 1.3238472424384464 9 0.21263205014566727 1.0832124412975985 >10 1.1259409353171643 11.707199190197413 >50 0.08548004808688695 3.3361526504295913 >100 0.03713104727062412 3.5811942319152217 >500 0.0026072036614861395 0.9939575967791661 >1k 8.343051716755647E-4 0.7521091424494212 >5k 0.0 0.0 >10k+ 1.0428814645944559E-4 0.6267281033973398 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10617 0.6276017067077777 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3936 0.23266839197530498 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.13790331256894E-4 0.0 3 0.0 0.0 0.0 5.320161401874352E-4 0.0 4 0.0 0.0 0.0 0.001536935516097035 0.0 5 1.1822580893054115E-4 0.0 0.0 0.002955645223263529 0.0 6 1.1822580893054115E-4 5.9112904465270575E-5 0.0 0.0043743549304300225 0.0 7 1.1822580893054115E-4 5.9112904465270575E-5 0.0 0.0075073388670893635 5.9112904465270575E-5 8 1.1822580893054115E-4 5.9112904465270575E-5 0.0 0.010640322803748704 5.9112904465270575E-5 9 1.1822580893054115E-4 5.9112904465270575E-5 0.0 0.014600887402921833 5.9112904465270575E-5 10 1.1822580893054115E-4 5.9112904465270575E-5 0.0 0.01962548428246983 5.9112904465270575E-5 11 1.1822580893054115E-4 5.9112904465270575E-5 0.0 0.026423468295975947 5.9112904465270575E-5 12 1.1822580893054115E-4 5.9112904465270575E-5 0.0 0.03464016201664856 5.9112904465270575E-5 13 1.1822580893054115E-4 5.9112904465270575E-5 0.0 0.04598983967398051 5.9112904465270575E-5 14 1.7733871339581175E-4 5.9112904465270575E-5 0.0 0.0604725012679718 5.9112904465270575E-5 15 1.7733871339581175E-4 5.9112904465270575E-5 0.0 0.07619653385573377 5.9112904465270575E-5 16 1.7733871339581175E-4 5.9112904465270575E-5 0.0 0.09156588901670412 5.9112904465270575E-5 17 1.7733871339581175E-4 5.9112904465270575E-5 0.0 0.10746726031786191 5.9112904465270575E-5 18 1.7733871339581175E-4 5.9112904465270575E-5 0.0 0.1235459703324155 5.9112904465270575E-5 19 1.7733871339581175E-4 5.9112904465270575E-5 0.0 0.13891532549338587 5.9112904465270575E-5 20 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.15877726139371678 5.9112904465270575E-5 21 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.18064903604586688 1.1822580893054115E-4 22 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.20364395588285714 1.1822580893054115E-4 23 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.2286487144716666 1.1822580893054115E-4 24 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.2530032311113581 1.1822580893054115E-4 25 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.28001782845198675 1.1822580893054115E-4 26 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.3075644419328028 1.1822580893054115E-4 27 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.3372391199743686 1.1822580893054115E-4 28 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.36815516900970513 1.1822580893054115E-4 29 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.3977707341468057 1.1822580893054115E-4 30 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.4332975897304333 1.1822580893054115E-4 31 2.955645223263529E-4 5.9112904465270575E-5 0.0 0.47118896149267175 1.1822580893054115E-4 32 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.5113857365290557 1.1822580893054115E-4 33 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.5539470277440506 1.1822580893054115E-4 34 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.5990501738510521 1.1822580893054115E-4 35 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.6481729974616919 1.1822580893054115E-4 36 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.7026159824742061 1.1822580893054115E-4 37 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.7580047739581646 1.1822580893054115E-4 38 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.8126842105885399 1.7733871339581175E-4 39 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.868191227881429 1.7733871339581175E-4 40 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.9296686485253104 1.7733871339581175E-4 41 3.546774267916235E-4 5.9112904465270575E-5 0.0 0.9959342144308787 1.7733871339581175E-4 42 3.546774267916235E-4 5.9112904465270575E-5 0.0 1.0668105868847382 1.7733871339581175E-4 43 3.546774267916235E-4 5.9112904465270575E-5 0.0 1.1451942982056869 2.364516178610823E-4 44 4.13790331256894E-4 5.9112904465270575E-5 0.0 1.2270065579856213 2.364516178610823E-4 45 4.13790331256894E-4 5.9112904465270575E-5 0.0 1.3159123663013883 2.364516178610823E-4 46 4.13790331256894E-4 5.9112904465270575E-5 0.0 1.4086014005029326 2.364516178610823E-4 47 4.13790331256894E-4 5.9112904465270575E-5 0.0 1.5073790638643998 2.364516178610823E-4 48 4.13790331256894E-4 5.9112904465270575E-5 0.0 1.6114768886277413 2.364516178610823E-4 49 4.13790331256894E-4 5.9112904465270575E-5 0.0 1.7128555197856803 3.546774267916235E-4 50 4.13790331256894E-4 5.9112904465270575E-5 0.0 1.813938586421293 3.546774267916235E-4 51 4.13790331256894E-4 5.9112904465270575E-5 0.0 1.9276127017080082 3.546774267916235E-4 52 4.13790331256894E-4 5.9112904465270575E-5 0.0 2.0460158493519454 3.546774267916235E-4 53 4.13790331256894E-4 5.9112904465270575E-5 0.0 2.1723992390986937 3.546774267916235E-4 54 4.13790331256894E-4 5.9112904465270575E-5 0.0 2.313147064630503 3.546774267916235E-4 55 4.13790331256894E-4 5.9112904465270575E-5 0.0 2.455372712773944 3.546774267916235E-4 56 4.729032357221646E-4 5.9112904465270575E-5 0.0 2.6070564256318285 3.546774267916235E-4 57 4.729032357221646E-4 5.9112904465270575E-5 0.0 2.766661267688059 3.546774267916235E-4 58 4.729032357221646E-4 5.9112904465270575E-5 0.0 2.9368473196435727 4.13790331256894E-4 59 4.729032357221646E-4 5.9112904465270575E-5 0.0 3.103604823140101 4.13790331256894E-4 60 4.729032357221646E-4 5.9112904465270575E-5 0.0 3.270953455681282 4.13790331256894E-4 61 4.729032357221646E-4 5.9112904465270575E-5 0.0 3.4505384594467743 4.13790331256894E-4 62 4.729032357221646E-4 5.9112904465270575E-5 0.0 3.6369214472257725 4.13790331256894E-4 63 4.729032357221646E-4 5.9112904465270575E-5 0.0 3.8333536287638665 4.729032357221646E-4 64 4.729032357221646E-4 5.9112904465270575E-5 0.0 4.039066536303008 4.729032357221646E-4 65 4.729032357221646E-4 5.9112904465270575E-5 0.0 4.263695573271036 4.729032357221646E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACGGT 50 0.006736057 28.393745 26 CGGTTAC 195 1.8189894E-12 25.483072 4 CTTACCG 80 0.0024569472 22.187204 23 TCGCGCT 135 5.341126E-6 21.032404 37 TCGTTAC 140 7.3107713E-6 20.283045 10 TAGCGCG 105 4.9748365E-4 20.282446 5 TATACCG 110 6.788123E-4 19.36796 46 CGCGTAA 115 9.1638457E-4 18.522587 52 CAATTCG 155 1.7593286E-5 18.32017 54 CGCGCTT 180 3.2022253E-6 17.74714 38 CTCGCGC 160 2.3136154E-5 17.74609 36 AATTCGC 165 3.0120687E-5 17.209349 55 GCGTAAC 125 0.00160324 17.040277 53 TCGCGGT 195 6.8641E-6 16.38149 33 CTTCGCG 200 8.729745E-6 15.971952 31 TTGCGTA 290 5.176844E-9 15.910288 36 CGCGCGT 135 0.0026843084 15.775236 8 ATTCGCG 180 6.346341E-5 15.775236 56 ATCGTAC 135 0.0026848377 15.774769 33 CGACCGC 135 0.0026853671 15.7743025 37 >>END_MODULE