FastQCFastQC Report
Thu 26 May 2016
SRR936006_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936006_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1691678
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115460.682517594956014No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87210.5155236398416246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62150.3673867012516566No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18240.10782193774465353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA13100.052.928295100-101
ACGTATC4250.044.111816100-101
GGTATCA88150.044.090991
CGTACAT13300.043.182617100-101
CGGTATC10050.042.342186100-101
CCGTACA5050.040.6594100-101
GCGTATC2950.038.3323100-101
AGGTATC19400.037.58094100-101
TCGAGTA3750.037.296654100-101
CCGTATC5850.036.11651100-101
TCGGTAT3300.036.070263100-101
AGTATCA27150.036.0603100-101
TCGTATC4450.035.442074100-101
GGGTATC12500.034.995373100-101
TACGCAG7700.034.78204100-101
CCCGTAC2850.034.45659698-99
GCGTACA4400.033.815876100-101
CGAGTAC9850.033.23225100-101
GTATCAA177450.032.9713331
ACCGTAT2600.032.04704398-99
Produced by FastQC (version 0.11.5)