FastQCFastQC Report
Thu 26 May 2016
SRR936005_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936005_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences992800
Sequences flagged as poor quality0
Sequence length77
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238542.402699435938759No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA135001.3597904915390815No Hit
CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG12620.12711522965350525No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTAT601.0983324E-535.5253971
TCGTTAG500.00673878728.3902528
CGGTATA807.890524E-526.61585810
TTCGCGA958.425917E-626.15418425
CGCGTCA958.43911E-626.1489142
TCGCGAT1001.25432325E-524.84647426
GCGGTAT901.7559227E-423.6585399
CGTCAAC1102.6276115E-522.5820124
CGGATTC1153.684598E-521.6143454
GTGCTAC850.00349522620.87833833
GTCGGAT1256.9999296E-519.88619652
GCGTCAA1257.040733E-519.871173
CCCGAGT1501.3209878E-518.9382849
GTGGTCG2305.0604285E-918.53218368
CGAGTGC1351.2632644E-418.41407451
GGTCGCC2501.4184479E-817.05132770
TATACCG2301.02443664E-716.9818446
CGAACTC2551.8291757E-816.7035125
CCCGTCA4900.016.6718267
GTAGCCG6550.016.26051123
Produced by FastQC (version 0.11.5)