##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936005_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 992800 Sequences flagged as poor quality 0 Sequence length 77 %GC 39 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 37.860056406124095 40.0 38.0 41.0 33.0 41.0 2 37.7123358178888 40.0 37.0 41.0 33.0 41.0 3 37.61349315068493 40.0 37.0 41.0 33.0 41.0 4 37.4984155922643 40.0 37.0 41.0 33.0 41.0 5 37.37150684931507 40.0 37.0 41.0 33.0 41.0 6 37.17452054794521 39.0 36.0 41.0 32.0 41.0 7 36.88724012892828 39.0 36.0 41.0 32.0 41.0 8 36.59329975825947 39.0 35.0 40.0 31.0 41.0 9 36.407585616438354 39.0 35.0 40.0 31.0 41.0 10-11 36.49305298146656 39.0 35.0 41.0 31.0 41.0 12-13 36.49014856970185 39.0 35.0 41.0 31.0 41.0 14-15 36.3006693190975 39.0 35.0 41.0 31.0 41.0 16-17 35.97809830781628 39.0 35.0 41.0 30.0 41.0 18-19 35.59338638195004 39.0 35.0 41.0 29.0 41.0 20-21 35.014140310233685 39.0 35.0 41.0 25.0 41.0 22-23 34.26699788477035 39.0 35.0 41.0 16.0 41.0 24-25 33.67342818291701 39.0 34.0 41.0 3.5 41.0 26-27 32.638237812248185 38.0 33.0 40.0 2.0 40.5 28-29 32.60108632151491 38.0 33.0 39.5 2.0 40.5 30-31 32.89883360193392 38.0 33.0 40.0 2.0 41.0 32-33 32.72730157131346 38.0 33.0 40.0 2.0 41.0 34-35 32.45877014504432 37.5 33.0 40.0 2.0 41.0 36-37 32.174776390008056 37.0 33.0 40.0 2.0 41.0 38-39 31.891663477034648 36.5 33.0 40.0 2.0 41.0 40-41 31.523828565672844 36.0 32.0 40.0 2.0 41.0 42-43 31.16286361804996 35.5 31.5 39.0 2.0 41.0 44-45 30.787905419016923 35.0 31.0 39.0 2.0 41.0 46-47 30.401810535858182 35.0 31.0 38.5 2.0 40.0 48-49 29.955013597904916 35.0 30.5 37.5 2.0 40.0 50-51 29.512850020145045 35.0 30.0 37.0 2.0 39.5 52-53 29.070371676067687 35.0 29.0 36.5 2.0 39.0 54-55 28.611514907332797 35.0 29.0 36.0 2.0 39.0 56-57 28.149655519742144 34.0 28.5 35.5 2.0 37.5 58-59 27.696425261885576 34.0 27.0 35.0 2.0 37.0 60-61 27.3081879532635 34.0 26.0 35.0 2.0 36.5 62-63 26.922449133763095 34.0 25.5 35.0 2.0 36.0 64-65 26.522392224012894 34.0 24.5 35.0 2.0 36.0 66-67 26.09494258662369 34.0 23.5 35.0 2.0 35.5 68-69 25.705953364222403 33.5 19.5 35.0 2.0 35.0 70-71 25.272620870265914 33.0 15.0 35.0 2.0 35.0 72-73 24.799882151490735 33.0 2.0 35.0 2.0 35.0 74-75 24.260564061240935 33.0 2.0 35.0 2.0 35.0 76-77 22.799344278807414 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24041789743687048 1101 2 0.16073683597335275 1101 3 0.25496513420545597 1101 4 0.3074424369160127 1101 5 0.4390787381578889 1101 6 0.2665612435012932 1101 7 0.3262827890790092 1101 8 0.5800813464274057 1101 9 0.5658622228555927 1101 10-11 0.41169855070204164 1101 12-13 0.43407073895862425 1101 14-15 0.44261998355793963 1101 16-17 0.46922557676821697 1101 18-19 0.5956250461634411 1101 20-21 0.7718334999866627 1101 22-23 0.6780887943431892 1101 24-25 0.44147365163529173 1101 26-27 0.3972876772336704 1101 28-29 0.4139458588550866 1101 30-31 0.32182210840640124 1101 32-33 0.2951254058695554 1101 34-35 0.31811933802094217 1101 36-37 0.29657608700758686 1101 38-39 0.3632438937320792 1101 40-41 0.37400797436319166 1101 42-43 0.30136116239454935 1101 44-45 0.25109877916924006 1101 46-47 0.29653823561668347 1101 48-49 0.3345077785705719 1101 50-51 0.35499474990885815 1101 52-53 0.4918914178895122 1101 54-55 0.4801942985893177 1101 56-57 0.6202188079897333 1101 58-59 0.5396414497230602 1101 60-61 0.4718253130987229 1101 62-63 0.3899913827847925 1101 64-65 0.4451144345664595 1101 66-67 0.5878387248839338 1101 68-69 0.4355297136231471 1101 70-71 0.6351933968447021 1101 72-73 0.7409775878532869 1101 74-75 0.688513123467029 1101 76-77 0.7656663485635136 1102 1 0.3924289582714593 1102 2 0.521049235991633 1102 3 0.4805341730097865 1102 4 0.5211644991674902 1102 5 0.3939841268868065 1102 6 0.6382087136781749 1102 7 0.51798373519172 1102 8 0.8220108221804097 1102 9 0.7391333961267605 1102 10-11 0.4902493299881172 1102 12-13 0.6260253529720643 1102 14-15 0.4712776004508541 1102 16-17 0.4334018416797747 1102 18-19 0.5413384014356168 1102 20-21 0.47755645350373044 1102 22-23 0.5524426267852576 1102 24-25 0.49834896586354205 1102 26-27 0.45189083551330356 1102 28-29 0.5198733464002245 1102 30-31 0.588964688048982 1102 32-33 0.4780942344266137 1102 34-35 0.5136464416362898 1102 36-37 0.46023701758028324 1102 38-39 0.7405634695810654 1102 40-41 0.7429422263856829 1102 42-43 0.9388103145495883 1102 44-45 0.8597554819435906 1102 46-47 0.8208497900752967 1102 48-49 0.995272667276641 1102 50-51 1.137231712292877 1102 52-53 1.120317648521624 1102 54-55 1.2493176588891473 1102 56-57 1.3734867818459406 1102 58-59 1.1917981982033368 1102 60-61 1.021872732999082 1102 62-63 0.8112023613487125 1102 64-65 0.9927005902408474 1102 66-67 1.0501061959749336 1102 68-69 1.1260925821271925 1102 70-71 1.2725534224400228 1102 72-73 1.4586846357073924 1102 74-75 1.3980936573122698 1102 76-77 1.1561240934330215 1103 1 0.34486790610245777 1103 2 0.19073460116322138 1103 3 0.17395440711681687 1103 4 0.10057997351673009 1103 5 0.08706535666211579 1103 6 0.05472518734826792 1103 7 0.060284513075401946 1103 8 0.12201938289585712 1103 9 0.14536217268638296 1103 10-11 0.3455886482127326 1103 12-13 0.2969548754553557 1103 14-15 0.42949845549511423 1103 16-17 0.5204477670645815 1103 18-19 0.29440627649483275 1103 20-21 0.31308113975350693 1103 22-23 0.2746514055135947 1103 24-25 0.38365250029759324 1103 26-27 0.21419914682657293 1103 28-29 0.16310039369532348 1103 30-31 0.4965517647929474 1103 32-33 0.5805300433286149 1103 34-35 0.49494127838344326 1103 36-37 0.5265409001500956 1103 38-39 0.6446806029969601 1103 40-41 0.48611733867372564 1103 42-43 0.4507582197425144 1103 44-45 0.36786076732930795 1103 46-47 0.25520279973748927 1103 48-49 0.40194089389590104 1103 50-51 0.5045235014253358 1103 52-53 0.5750520095212863 1103 54-55 0.6929687469560832 1103 56-57 0.6876621851983167 1103 58-59 0.6567529120186215 1103 60-61 0.6193233519388066 1103 62-63 0.7503624980918744 1103 64-65 0.7663598395231759 1103 66-67 0.6596422622691129 1103 68-69 0.7549787189045958 1103 70-71 0.6454051108984622 1103 72-73 0.7008963821504182 1103 74-75 0.5220693677089727 1103 76-77 0.6791370086990582 1104 1 0.3939876890192764 1104 2 0.3874651112032055 1104 3 0.32057420967257144 1104 4 0.31327637689821586 1104 5 0.3848746614774754 1104 6 0.3019389835320325 1104 7 0.4286952294252089 1104 8 0.4727617907640038 1104 9 0.5815002826576858 1104 10-11 0.5187703914095252 1104 12-13 0.6142353430191321 1104 14-15 0.5820278942592267 1104 16-17 0.6068994827780827 1104 18-19 0.8783486973807797 1104 20-21 0.7478961465934759 1104 22-23 0.7881702875082865 1104 24-25 0.9712567409609676 1104 26-27 0.9637342651707996 1104 28-29 0.9327334373824172 1104 30-31 1.051396577925665 1104 32-33 0.9968732286741613 1104 34-35 1.0586370791301718 1104 36-37 1.0859088406542199 1104 38-39 1.0768011479044723 1104 40-41 1.0945096729800277 1104 42-43 1.252755106690703 1104 44-45 1.0466715905149222 1104 46-47 0.9274850796944385 1104 48-49 1.1654987732051048 1104 50-51 1.0359901258963475 1104 52-53 0.9832921701341135 1104 54-55 1.1321868031054798 1104 56-57 1.1989955342428011 1104 58-59 1.0654054902741699 1104 60-61 1.1059840259246272 1104 62-63 1.2832784759930185 1104 64-65 1.1113648727017527 1104 66-67 1.081738533452512 1104 68-69 0.9548312585556076 1104 70-71 1.117870201288067 1104 72-73 1.1591254888481899 1104 74-75 1.054057826748327 1104 76-77 0.9910397746467154 1105 1 -0.33530213416551646 1105 2 -0.022323567675286426 1105 3 -0.3222542150727179 1105 4 -0.3661731631701741 1105 5 -0.25985468136376255 1105 6 -0.41394942230348875 1105 7 -0.46242115403669715 1105 8 -0.2290204994391445 1105 9 -0.29667638233007665 1105 10-11 -0.17928848513793127 1105 12-13 -0.22521605268110534 1105 14-15 -0.49405853400293154 1105 16-17 -0.4048048046151038 1105 18-19 -0.3827066711388696 1105 20-21 -0.3343801393152148 1105 22-23 -0.44358487512459277 1105 24-25 -0.29051658035494654 1105 26-27 -0.42977455791679375 1105 28-29 -0.4118684412239162 1105 30-31 -0.3997671683299373 1105 32-33 -0.29333433700195144 1105 34-35 -0.4135880885394201 1105 36-37 -0.33038559510115206 1105 38-39 -0.35913095364277225 1105 40-41 -0.3510512801078285 1105 42-43 -0.5024616487812033 1105 44-45 -0.6049981467806269 1105 46-47 -0.6894920739430397 1105 48-49 -0.49221778344910305 1105 50-51 -0.56440121360475 1105 52-53 -0.4306468281781477 1105 54-55 -0.4264080859542432 1105 56-57 -0.366009016473388 1105 58-59 -0.46011165664769393 1105 60-61 -0.6134786802935075 1105 62-63 -0.8114962635557923 1105 64-65 -0.696575574770609 1105 66-67 -0.5828769973023782 1105 68-69 -0.6761514973227705 1105 70-71 -0.747585996964105 1105 72-73 -0.8193138364087247 1105 74-75 -0.7797902235127907 1105 76-77 -0.45833350778928406 1106 1 -0.07479793248484157 1106 2 0.044903323081015856 1106 3 -0.24977522347607817 1106 4 0.015129357838226554 1106 5 -0.2756109838847749 1106 6 -0.21331917020264513 1106 7 0.04178052663556997 1106 8 -0.006331423808894954 1106 9 -0.10760075207797115 1106 10-11 -0.06846915740684523 1106 12-13 -0.12227487621051836 1106 14-15 0.09575239036681893 1106 16-17 0.08416578362018612 1106 18-19 0.00857484146617793 1106 20-21 -0.044989383012691064 1106 22-23 0.06166722571574468 1106 24-25 0.1165212347710991 1106 26-27 -0.014333381446213878 1106 28-29 -0.0085070966861025 1106 30-31 -0.29472515152321677 1106 32-33 -0.1573049252372556 1106 34-35 -0.03963850870748331 1106 36-37 -0.016309964849057224 1106 38-39 -0.04925700406294098 1106 40-41 -0.0354000196036246 1106 42-43 -0.09069694289884822 1106 44-45 -0.3634015081251647 1106 46-47 -0.26722316638001686 1106 48-49 -0.2595497162222138 1106 50-51 -0.32332978503332654 1106 52-53 -0.33453338279999656 1106 54-55 -0.08817279183659821 1106 56-57 -0.5403787643725444 1106 58-59 -0.6418343457233249 1106 60-61 -0.5740879239909837 1106 62-63 -0.814122313975961 1106 64-65 -0.7727310369554843 1106 66-67 -0.561868593941032 1106 68-69 -0.3620758670706685 1106 70-71 -0.6346658288968747 1106 72-73 -0.6958894666608302 1106 74-75 -0.8449162739329594 1106 76-77 -0.8075982136716391 1107 1 -0.34519237256704116 1107 2 -0.3228084733671821 1107 3 -0.12524718164304716 1107 4 -0.2741768218901228 1107 5 -0.3407996836191387 1107 6 -0.67014543947775 1107 7 -0.49958890384039734 1107 8 -0.2541971705051864 1107 9 -0.37509035229181364 1107 10-11 -0.2688665628899116 1107 12-13 -0.2746066836850005 1107 14-15 -0.2294883918307562 1107 16-17 -0.099200405797923 1107 18-19 -0.3108750557889479 1107 20-21 -0.5393666070633429 1107 22-23 -0.41033897238974504 1107 24-25 -0.776209565302409 1107 26-27 -0.7441404633246833 1107 28-29 -0.4160283556867199 1107 30-31 -0.3386966168488996 1107 32-33 -0.25538138246029973 1107 34-35 -0.286998884269245 1107 36-37 -0.3307677559177655 1107 38-39 -0.2551122089679616 1107 40-41 -0.33498486348153733 1107 42-43 -0.31200564530625385 1107 44-45 -0.5203537025218097 1107 46-47 -0.46756836410125047 1107 48-49 -0.33851604430414284 1107 50-51 -0.36111776304759147 1107 52-53 -0.33812527681406124 1107 54-55 -0.42748356584799296 1107 56-57 -0.48901467997139036 1107 58-59 -0.48238557615585265 1107 60-61 -0.48106100149236397 1107 62-63 -0.4949574711004452 1107 64-65 -0.5688158724801511 1107 66-67 -0.6233004231339443 1107 68-69 -0.7437344450147663 1107 70-71 -0.4127327216579566 1107 72-73 -0.5243566885396405 1107 74-75 -0.6276359224285386 1107 76-77 -0.29890562132655063 1108 1 -0.2926016348102607 1108 2 -0.23393184641417264 1108 3 -0.23499037029691294 1108 4 -0.1550235642987019 1108 5 -0.25636806529612244 1108 6 -0.2825257405753874 1108 7 -0.3929895708988056 1108 8 -0.19452431167464823 1108 9 -0.4251770616955355 1108 10-11 -0.5008392990783435 1108 12-13 -0.5548218908969318 1108 14-15 -0.4619325528174656 1108 16-17 -0.3980949888659424 1108 18-19 -0.45171743713424206 1108 20-21 -0.8262959066520139 1108 22-23 -0.7567507181868436 1108 24-25 -0.6880265112067931 1108 26-27 -0.8600638724067551 1108 28-29 -1.0129099906964356 1108 30-31 -0.9302282714117425 1108 32-33 -0.8281031363857707 1108 34-35 -0.8138317081293778 1108 36-37 -0.8515227317271723 1108 38-39 -1.0021352202309721 1108 40-41 -0.7592293041495743 1108 42-43 -0.828426174653341 1108 44-45 -1.0622797978550622 1108 46-47 -1.260202876286634 1108 48-49 -1.3478104324551516 1108 50-51 -1.0932077156082336 1108 52-53 -1.1504859224152213 1108 54-55 -1.100199075058434 1108 56-57 -1.10191759205604 1108 58-59 -1.136290161005153 1108 60-61 -1.0068583287543333 1108 62-63 -0.864518682616108 1108 64-65 -0.9367155803093858 1108 66-67 -0.8207122446866073 1108 68-69 -0.5244804530039104 1108 70-71 -0.4272235316896307 1108 72-73 -0.6127434636664262 1108 74-75 -0.5082149610700171 1108 76-77 -0.6084116131818043 1109 1 -0.3532903195386723 1109 2 -0.15808985438798828 1109 3 -0.4580267419052362 1109 4 -0.3830818078161542 1109 5 -0.23904388179641245 1109 6 -0.46045703524965376 1109 7 -0.6596463807610462 1109 8 -0.3972458093650957 1109 9 -0.28921943915676707 1109 10-11 -0.5466464184102549 1109 12-13 -0.3835923735064739 1109 14-15 -0.2656181479475208 1109 16-17 -0.27716627395531646 1109 18-19 -0.4077482719375993 1109 20-21 -0.4207121968907188 1109 22-23 -0.4239326578020197 1109 24-25 -0.5384303002983657 1109 26-27 -0.6764672944173356 1109 28-29 -0.6277599463408166 1109 30-31 -0.6436185702768498 1109 32-33 -0.5341946415198038 1109 34-35 -0.5724948195486519 1109 36-37 -0.48976033509616457 1109 38-39 -0.5820446735861928 1109 40-41 -0.4844681617119555 1109 42-43 -0.4586456075132084 1109 44-45 -0.6067797009864684 1109 46-47 -0.6540721138798062 1109 48-49 -0.4247225675409574 1109 50-51 -0.45141656429948895 1109 52-53 -0.46683224293811065 1109 54-55 -0.3372762946238481 1109 56-57 -0.14226744063513053 1109 58-59 -0.19919560705953998 1109 60-61 -0.20172021453096178 1109 62-63 -0.20762010913363227 1109 64-65 -0.029624289305932194 1109 66-67 0.10488196766975122 1109 68-69 -0.24322758518365362 1109 70-71 -0.29247013873962757 1109 72-73 -0.15323096761764532 1109 74-75 -0.12225631886181887 1109 76-77 0.027773905473047478 1110 1 0.07003788554112589 1110 2 -0.1374582297383924 1110 3 -0.1315904376456345 1110 4 0.15319602397084253 1110 5 0.1554478076013197 1110 6 0.06978959284499098 1110 7 0.05729950829878305 1110 8 -0.02054664278495011 1110 9 -0.004171916291994648 1110 10-11 -0.02756034869747026 1110 12-13 0.027050011037033528 1110 14-15 -0.014787559645597526 1110 16-17 -0.12709784480544073 1110 18-19 0.12723176562763427 1110 20-21 -0.02574386306972798 1110 22-23 -0.11681508236478777 1110 24-25 -0.1716448657968641 1110 26-27 -0.19050706827079722 1110 28-29 -0.14827406162270051 1110 30-31 -0.06993123014150626 1110 32-33 0.13967108633038805 1110 34-35 0.2262069143044272 1110 36-37 0.27646586807706086 1110 38-39 0.14379700495725345 1110 40-41 0.10494372513926464 1110 42-43 0.11957834929619793 1110 44-45 0.1289596641797921 1110 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-0.34774985406140146 2313 48-49 -0.335938184143199 2313 50-51 -0.17452720719425585 2313 52-53 -0.2448848994210735 2313 54-55 0.008553951159971263 2313 56-57 0.29888327046844054 2313 58-59 0.0273647586253567 2313 60-61 0.11617120490854305 2313 62-63 0.02526474833318204 2313 64-65 0.20498944709025224 2313 66-67 0.3407018571429141 2313 68-69 0.1149735415115174 2313 70-71 -0.015546855789150271 2313 72-73 0.016267465543975845 2313 74-75 0.06254445607590853 2313 76-77 -0.3006028321105383 2314 1 0.07866240452954543 2314 2 -0.0673083155011156 2314 3 -0.10060272725245767 2314 4 -0.012214564023707908 2314 5 0.01528024171541631 2314 6 0.11823596494041766 2314 7 0.09375157683575708 2314 8 -0.05678825575633084 2314 9 0.24754209039932817 2314 10-11 -0.14235512540660267 2314 12-13 -0.09672198489693073 2314 14-15 -0.11272301550233266 2314 16-17 -0.5011144017523961 2314 18-19 -0.23231326770419258 2314 20-21 0.020348401315700926 2314 22-23 0.06912282575680706 2314 24-25 -0.14095911338910128 2314 26-27 -0.10038259170622865 2314 28-29 -0.20766162760841667 2314 30-31 -0.24662947346141095 2314 32-33 -0.37017083076176505 2314 34-35 -0.49448694022668604 2314 36-37 -0.5937587454083975 2314 38-39 -0.5317811655599982 2314 40-41 -0.39365379231690056 2314 42-43 -0.401641477780867 2314 44-45 -0.36167922235221894 2314 46-47 -0.38022766137173036 2314 48-49 -0.2446247671850088 2314 50-51 -0.2193748522748784 2314 52-53 -0.2498104884066379 2314 54-55 -0.307215644579788 2314 56-57 -0.16613953988694163 2314 58-59 -0.36391663413607134 2314 60-61 -0.37268681380869495 2314 62-63 -0.3964620561555563 2314 64-65 -0.6923564787552401 2314 66-67 -0.5218628010479627 2314 68-69 -0.4301066134006142 2314 70-71 -0.34398725380191664 2314 72-73 -0.5921627665567009 2314 74-75 -0.5192860218321158 2314 76-77 -0.44146995608588213 2315 1 -0.4663945711403059 2315 2 -0.4363345566862762 2315 3 -0.411362165945377 2315 4 -0.5027762659497483 2315 5 -0.372120356864734 2315 6 -0.3505202044625051 2315 7 -0.553015853454923 2315 8 -0.47827712516635756 2315 9 -0.23092007980906715 2315 10-11 -0.2916511225005678 2315 12-13 -0.4208689292163399 2315 14-15 -0.6395821280559417 2315 16-17 -0.3437269119195676 2315 18-19 -0.2888960699753227 2315 20-21 -0.05062125114456251 2315 22-23 0.07128261033113148 2315 24-25 -0.09079130562966498 2315 26-27 -0.06254766716049431 2315 28-29 0.045048171575047036 2315 30-31 0.025410595083123155 2315 32-33 0.2066010217569314 2315 34-35 0.29665754817680323 2315 36-37 0.09577794723885802 2315 38-39 0.19568481882005884 2315 40-41 0.05713391704795967 2315 42-43 0.17056679336488756 2315 44-45 0.06748892497115122 2315 46-47 0.2082212421526286 2315 48-49 0.3777078791236157 2315 50-51 0.3435254185865837 2315 52-53 0.2016010709815177 2315 54-55 0.19826870784019945 2315 56-57 0.06850940628033086 2315 58-59 0.17715401885327609 2315 60-61 0.20192209540138606 2315 62-63 0.029672126877301253 2315 64-65 0.0031901007303609674 2315 66-67 0.04105642221927397 2315 68-69 -0.21179306164080458 2315 70-71 -0.14982419993760132 2315 72-73 -1.3843429249672283 2315 74-75 -2.0434941666284985 2315 76-77 -1.935402027504864 2316 1 -2.2469132302168404 2316 2 -2.1023249230561447 2316 3 -1.4456345349426059 2316 4 -1.4108578112229466 2316 5 -1.9684274653160543 2316 6 -1.481887888012352 2316 7 -2.124854514477647 2316 8 -1.7089247191915646 2316 9 -1.2674159684871213 2316 10-11 -1.4133876086583044 2316 12-13 -1.4553213205947273 2316 14-15 -1.6060152521740676 2316 16-17 -2.6587003951093706 2316 18-19 -2.6366847139689042 2316 20-21 -1.914074275034018 2316 22-23 -2.1185509906030404 2316 24-25 -2.2364075173749143 2316 26-27 -1.8394610131440459 2316 28-29 -1.7976098345554448 2316 30-31 -2.1167049111690055 2316 32-33 -2.271051875630313 2316 34-35 -2.2249913595252337 2316 36-37 -2.2608026006130366 2316 38-39 -2.1536778046412337 2316 40-41 -2.120915516124814 2316 42-43 -2.1711229842831443 2316 44-45 -1.9321656266758112 2316 46-47 -1.7245501294232959 2316 48-49 -1.7844266024605808 2316 50-51 -2.287102713559573 2316 52-53 -3.101687986576991 2316 54-55 -2.3065991946557887 2316 56-57 -2.0299604034798016 2316 58-59 -1.946810852455048 2316 60-61 -1.9149854120184564 2316 62-63 -1.9229187357704873 2316 64-65 -1.9081561747525377 2316 66-67 -1.9946123365993884 2316 68-69 -1.909569541959982 2316 70-71 -1.5604090294028872 2316 72-73 -1.5022089299300063 2316 74-75 -1.8526193551654515 2316 76-77 -1.9994218786214901 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15075.0 3 2106.0 4 4132.0 5 5556.0 6 6106.0 7 6456.0 8 9085.0 9 10603.0 10 17447.0 11 21497.0 12 13169.0 13 7179.0 14 5877.0 15 5487.0 16 5301.0 17 5235.0 18 5475.0 19 5551.0 20 5903.0 21 6069.0 22 6711.0 23 7665.0 24 8571.0 25 9727.0 26 11216.0 27 12905.0 28 15482.0 29 18283.0 30 21719.0 31 26983.0 32 33949.0 33 44234.0 34 65184.0 35 88308.0 36 112334.0 37 150527.0 38 146544.0 39 48558.0 40 591.0 >>END_MODULE >>Per base sequence content pass #Base G A T C 1 19.229603035733533 26.180582627940314 35.86408504405622 18.72572929226994 2 19.12860395301513 26.65635766526303 35.7489976426974 18.46604073902444 3 18.985404754133373 26.914467507243224 35.45613165540391 18.6439960832195 4 19.149977887094224 26.670582178912046 35.39163013923575 18.787809794757983 5 18.924769961981017 27.00355206505336 35.46060995585607 18.61106801710955 6 19.133210704564732 26.76393021845516 35.624328849257274 18.478530227722832 7 19.148198818084555 26.907012308764834 35.28076032664871 18.664028546501903 8 18.643079139444797 27.185565400482563 35.14152012613148 19.029835333941154 9 18.777874603950174 27.036162056789394 35.17899689204777 19.006966447212665 10-11 19.00803257762827 27.20754838638179 35.09857613922463 18.68584289676531 12-13 19.065424232700984 27.127359220004806 34.64089786489614 19.166318682398074 14-15 19.06052498504101 27.510793642479104 34.52071795099092 18.90796342148897 16-17 19.226720578443548 27.657184761663007 34.175150821016324 18.940943838877118 18-19 19.048312254565165 27.814353039311886 33.715719717194595 19.421614988928358 20-21 19.163621947367705 27.98827885917856 33.300321238245736 19.547777955207994 22-23 19.554652199537575 28.274220603569432 32.29988061596119 19.8712465809318 24-25 19.51266566561676 28.821623886978408 31.320957723691084 20.344752723713754 26-27 19.739228950694496 29.425881549312304 30.59417776217273 20.240711737820465 28-29 19.781212743634907 29.69478679096746 30.224000314294507 20.300000151103127 30-31 19.712141656605827 29.558819011182013 30.138897493277245 20.590141838934915 32-33 19.88770757024447 29.826197067321058 29.773964516116035 20.512130846318435 34-35 19.723946249958445 29.50243233142165 30.19989543342242 20.573725985197484 36-37 19.88103914707617 29.58250875534349 30.315675905465966 20.22077619211437 38-39 20.123528988058627 29.481691023395655 30.383325702179487 20.011454286366227 40-41 19.875739793990984 29.72362555720654 30.355806281008384 20.044828367794093 42-43 19.81610830408921 29.893280558747072 29.997647709488596 20.292963427675126 44-45 20.017800674753364 29.743043683681908 30.011867116502245 20.227288525062484 46-47 19.71719593777706 29.961362537277342 30.019545417909242 20.301896107036352 48-49 19.74131078471178 30.017650952415977 29.703671539148946 20.537366723723302 50-51 19.391960824321565 30.231925457389647 29.77618576203899 20.599927956249793 52-53 19.054981960862552 30.237886874128083 29.91085980761276 20.796271357396602 54-55 18.99396479632447 30.126144824737285 29.88352762188794 20.996362757050306 56-57 18.920322572357893 30.297551792871836 29.72490475612379 21.057220878646483 58-59 19.061012951910246 30.164305355400558 29.373127141084804 21.40155455160439 60-61 19.114683162356783 29.68564325887269 29.510695584498016 21.688977994272516 62-63 18.923106994101893 29.724278152020812 29.424738192201282 21.927876661676013 64-65 18.962400022971355 29.498695519225667 29.582722317124592 21.95618214067839 66-67 19.030557909298558 29.525223448855375 29.450362666736524 21.993855975109547 68-69 19.088273585438014 29.249026594329656 29.585435997065673 22.07726382316665 70-71 19.017131593148974 29.03910602702135 29.676974310420494 22.26678806940918 72-73 19.244060388070775 28.9174522976177 29.631929547250763 22.206557767060758 74-75 19.25994512063581 28.655516061589083 29.596751593255604 22.487787224519504 76-77 19.324217448483086 28.439564627194517 30.034079974272416 22.202137950049977 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 29856.0 1 17377.5 2 4537.5 3 4111.5 4 4047.0 5 4023.0 6 4069.5 7 4223.0 8 4357.0 9 4408.0 10 4430.5 11 4446.0 12 4708.5 13 4978.0 14 5127.5 15 5548.5 16 6113.0 17 6406.0 18 6812.5 19 7593.0 20 8402.0 21 9464.0 22 10091.0 23 10672.5 24 12090.5 25 14126.5 26 15326.0 27 16333.5 28 18387.5 29 20638.0 30 21842.0 31 23320.0 32 26635.5 33 29600.0 34 31478.0 35 32229.0 36 33059.5 37 34168.5 38 34483.0 39 34519.0 40 34535.5 41 35061.0 42 35812.5 43 36055.0 44 36020.0 45 36451.0 46 36465.0 47 35867.5 48 35722.0 49 35683.5 50 34985.0 51 33249.5 52 32174.0 53 31126.0 54 28701.5 55 26036.5 56 23234.5 57 21721.0 58 21453.0 59 19154.5 60 15984.0 61 14844.0 62 13604.5 63 9824.0 64 6086.5 65 4890.0 66 4365.0 67 3392.0 68 2615.5 69 1907.0 70 1527.0 71 1338.5 72 1080.5 73 911.5 74 812.0 75 809.0 76 611.0 77 332.5 78 249.0 79 200.5 80 148.5 81 119.0 82 75.0 83 57.0 84 51.5 85 34.5 86 23.5 87 24.0 88 24.0 89 22.0 90 18.5 91 17.0 92 15.0 93 12.0 94 11.0 95 8.5 96 6.0 97 10.0 98 14.5 99 28.0 100 41.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009165995165189364 2 0.014101531023368252 3 0.014907332796132152 4 0.016821112006446415 5 0.013900080580177276 6 0.010273972602739727 7 0.017526188557614827 8 0.018634165995165188 9 0.018634165995165188 10-11 0.02185737308622079 12-13 0.01797945205479452 14-15 0.008662369057211926 16-17 0.006748589846897663 18-19 0.017526188557614827 20-21 0.023620064464141822 22-23 0.02064867042707494 24-25 0.017677276390008056 26-27 0.02452659145850121 28-29 0.010122884770346495 30-31 0.009014907332796132 32-33 0.012489927477840453 34-35 0.013195004029008863 36-37 0.012137389202256245 38-39 0.01596494762288477 40-41 0.012590652699435937 42-43 0.01515914585012087 44-45 0.014202256244963738 46-47 0.024174053182917002 48-49 0.022058823529411766 50-51 0.03500201450443191 52-53 0.03772159548751007 54-55 0.02921031426269138 56-57 0.021807010475423046 58-59 0.01677074939564867 60-61 0.02190773569701853 62-63 0.02800161160354553 64-65 0.026138195004029006 66-67 0.029311039484286865 68-69 0.04109589041095891 70-71 0.05142022562449638 72-73 0.08219178082191782 74-75 0.08118452860596294 76-77 0.0874798549556809 >>END_MODULE >>Sequence Length Distribution pass #Length Count 77 992800.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.65034670950289 #Duplication Level Percentage of deduplicated Percentage of total 1 81.23609597888756 50.894695784037594 2 10.578195915537222 13.254552833389086 3 3.557530717437446 6.686415986314875 4 1.6598196445750706 4.159531048314881 5 0.8604703919086399 2.695438419316906 6 0.550705085942157 2.070111874137763 7 0.35645152166660826 1.563226799228002 8 0.21457554088955463 1.075458562568777 9 0.15513469484268633 0.8747318176710505 >10 0.7667745716723374 8.272265368448924 >50 0.04096245247504406 1.7427638327122248 >100 0.022159454107859404 2.5181501077844195 >500 6.423028901849357E-4 0.30783981553390516 >1k 1.6057572254623393E-4 0.12695877359600924 >5k 0.0 0.0 >10k+ 3.2115144509246786E-4 3.7578589769455855 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23854 2.402699435938759 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13500 1.3597904915390815 No Hit CCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG 1262 0.12711522965350525 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.01450443190975E-4 0.0 2 0.0 0.0 0.0 0.002115229653505238 0.0 3 0.0 0.0 0.0 0.0031224818694601127 0.0 4 0.0 0.0 0.0 0.006244963738920225 0.0 5 0.0 0.0 0.0 0.012792103142626915 0.0 6 0.0 0.0 0.0 0.02145447219983884 0.0 7 0.0 0.0 0.0 0.03767123287671233 0.0 8 0.0 0.0 0.0 0.051974214343271555 0.0 9 0.0 0.0 0.0 0.06637792103142627 0.0 10 0.0 0.0 0.0 0.0871273166800967 0.0 11 0.0 0.0 0.0 0.11059629331184528 0.0 12 0.0 0.0 0.0 0.14071313456889606 0.0 13 0.0 0.0 0.0 0.18201047542304594 0.0 14 0.0 0.0 0.0 0.23056003223207092 0.0 15 0.0 0.0 0.0 0.2876712328767123 0.0 16 0.0 0.0 0.0 0.34649476228847703 0.0 17 0.0 0.0 0.0 0.39867042707493955 0.0 18 0.0 0.0 0.0 0.4502417405318292 0.0 19 0.0 0.0 0.0 0.5078565672844481 0.0 20 0.0 1.007252215954875E-4 0.0 0.5699033037872683 0.0 21 0.0 1.007252215954875E-4 0.0 0.6383964544721998 0.0 22 0.0 1.007252215954875E-4 0.0 0.7111200644641418 0.0 23 0.0 1.007252215954875E-4 0.0 0.7917002417405318 0.0 24 0.0 1.007252215954875E-4 0.0 0.8690572119258663 0.0 25 0.0 1.007252215954875E-4 0.0 0.9549758259468171 0.0 26 0.0 1.007252215954875E-4 0.0 1.0494560838033844 0.0 27 0.0 1.007252215954875E-4 0.0 1.1428283642224013 0.0 28 0.0 1.007252215954875E-4 0.0 1.234085414987913 0.0 29 0.0 1.007252215954875E-4 0.0 1.3209105560032233 0.0 30 0.0 1.007252215954875E-4 0.0 1.4097502014504433 0.0 31 0.0 1.007252215954875E-4 0.0 1.5057413376309428 0.0 32 0.0 1.007252215954875E-4 0.0 1.613215149073328 0.0 33 0.0 1.007252215954875E-4 0.0 1.7319701853344078 0.0 34 0.0 1.007252215954875E-4 0.0 1.8471998388396456 0.0 35 0.0 1.007252215954875E-4 0.0 1.968372280419017 0.0 36 0.0 1.007252215954875E-4 0.0 2.103948428686543 0.0 37 0.0 1.007252215954875E-4 0.0 2.242546333601934 0.0 38 0.0 1.007252215954875E-4 0.0 2.3794319097502012 0.0 39 0.0 1.007252215954875E-4 0.0 2.5117848509266723 0.0 40 0.0 1.007252215954875E-4 0.0 2.654613215149073 0.0 41 0.0 1.007252215954875E-4 0.0 2.8027800161160354 0.0 42 0.0 1.007252215954875E-4 0.0 2.9627316680096696 0.0 43 0.0 1.007252215954875E-4 0.0 3.135777598710717 0.0 44 0.0 1.007252215954875E-4 0.0 3.319802578565673 0.0 45 0.0 1.007252215954875E-4 0.0 3.5122884770346494 0.0 46 0.0 1.007252215954875E-4 0.0 3.7226027397260273 0.0 47 0.0 1.007252215954875E-4 0.0 3.9452054794520546 0.0 48 0.0 1.007252215954875E-4 0.0 4.160656728444803 0.0 49 0.0 1.007252215954875E-4 0.0 4.382453666398066 0.0 50 0.0 1.007252215954875E-4 0.0 4.595588235294118 0.0 51 0.0 1.007252215954875E-4 0.0 4.821716357775987 0.0 52 0.0 1.007252215954875E-4 0.0 5.061946011281225 0.0 53 0.0 1.007252215954875E-4 0.0 5.31607574536664 0.0 54 0.0 1.007252215954875E-4 0.0 5.590149073327962 0.0 55 0.0 1.007252215954875E-4 0.0 5.863920225624496 0.0 56 0.0 1.007252215954875E-4 0.0 6.158138597904915 0.0 57 0.0 1.007252215954875E-4 0.0 6.466055600322321 0.0 58 0.0 1.007252215954875E-4 0.0 6.775382755842063 0.0 59 0.0 1.007252215954875E-4 0.0 7.0917606768734895 0.0 60 0.0 1.007252215954875E-4 0.0 7.402699435938759 0.0 61 0.0 1.007252215954875E-4 0.0 7.709004834810637 0.0 62 0.0 1.007252215954875E-4 0.0 8.035455278001612 0.0 63 0.0 1.007252215954875E-4 0.0 8.37076954069299 0.0 64 0.0 1.007252215954875E-4 0.0 8.728243352135374 0.0 65 0.0 1.007252215954875E-4 0.0 9.088940370668816 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCGTAT 60 1.0983324E-5 35.52539 71 TCGTTAG 50 0.006738787 28.39025 28 CGGTATA 80 7.890524E-5 26.615858 10 TTCGCGA 95 8.425917E-6 26.154184 25 CGCGTCA 95 8.43911E-6 26.148914 2 TCGCGAT 100 1.25432325E-5 24.846474 26 GCGGTAT 90 1.7559227E-4 23.658539 9 CGTCAAC 110 2.6276115E-5 22.582012 4 CGGATTC 115 3.684598E-5 21.61434 54 GTGCTAC 85 0.003495226 20.878338 33 GTCGGAT 125 6.9999296E-5 19.886196 52 GCGTCAA 125 7.040733E-5 19.87117 3 CCCGAGT 150 1.3209878E-5 18.93828 49 GTGGTCG 230 5.0604285E-9 18.532183 68 CGAGTGC 135 1.2632644E-4 18.414074 51 GGTCGCC 250 1.4184479E-8 17.051327 70 TATACCG 230 1.02443664E-7 16.98184 46 CGAACTC 255 1.8291757E-8 16.70351 25 CCCGTCA 490 0.0 16.67182 67 GTAGCCG 655 0.0 16.260511 23 >>END_MODULE